bhf-ucl Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. J. Biol. Chem. (0021-9258) 1999 imex curation Zhang S.H., Liu J., Kobayashi R., Tonks N.K. Cardiac - Interactions involving cardiac related proteins Species of PTPH1 construct confirmed as human from first line of abstract: The human band 4.1-related protein-tyrosine phosphatase PTPH1 was introduced into NIH3T3 cells. VCP construct confirmed as mouse in methods: Myc-tagged murine VCP constructs tonks@cshl.org 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang S.H., Liu J., Kobayashi R., Tonks N.K. Cardiac - Interactions involving cardiac related proteins Species of PTPH1 construct confirmed as human from first line of abstract: The human band 4.1-related protein-tyrosine phosphatase PTPH1 was introduced into NIH3T3 cells. VCP construct confirmed as mouse in methods: Myc-tagged murine VCP constructs tonks@cshl.org Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1999 imex curation Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. J. Biol. Chem. (0021-9258) 1999 imex curation Zhang S.H., Liu J., Kobayashi R., Tonks N.K. Cardiac - Interactions involving cardiac related proteins Species of PTPH1 construct confirmed as human from first line of abstract: The human band 4.1-related protein-tyrosine phosphatase PTPH1 was introduced into NIH3T3 cells. VCP construct confirmed as mouse in methods: Myc-tagged murine VCP constructs tonks@cshl.org 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip polyclonal western polyclonal antibody western blot polyclonal western Zhang S.H., Liu J., Kobayashi R., Tonks N.K. Cardiac - Interactions involving cardiac related proteins Species of PTPH1 construct confirmed as human from first line of abstract: The human band 4.1-related protein-tyrosine phosphatase PTPH1 was introduced into NIH3T3 cells. VCP construct confirmed as mouse in methods: Myc-tagged murine VCP constructs tonks@cshl.org Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1999 imex curation Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. J. Biol. Chem. (0021-9258) 1999 imex curation Zhang S.H., Liu J., Kobayashi R., Tonks N.K. Cardiac - Interactions involving cardiac related proteins Species of PTPH1 construct confirmed as human from first line of abstract: The human band 4.1-related protein-tyrosine phosphatase PTPH1 was introduced into NIH3T3 cells. VCP construct confirmed as mouse in methods: Myc-tagged murine VCP constructs tonks@cshl.org 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down edman degradation edman degradation Zhang S.H., Liu J., Kobayashi R., Tonks N.K. Cardiac - Interactions involving cardiac related proteins Species of PTPH1 construct confirmed as human from first line of abstract: The human band 4.1-related protein-tyrosine phosphatase PTPH1 was introduced into NIH3T3 cells. VCP construct confirmed as mouse in methods: Myc-tagged murine VCP constructs tonks@cshl.org Accepted 2013-SEP-03 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1999 imex curation tera_human Transitional endoplasmic reticulum ATPase VCP 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein human Homo sapiens Human MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein P55072 original sequence version: 89 DIP protein P55072 original sequence version: 114 DIP protein P55072 original sequence version: 123 DIP protein P55072 original sequence version: 150 DIP protein P55072 original sequence version: 174 DIP protein P55072 original sequence version: 175 DIP protein P55072 original sequence version: 176 DIP protein P55072 original sequence version: 160 DIP protein P55072 original sequence version: 128 DIP protein P55072 original sequence version: 132 501B721D3A77BA8A ptn3_human Tyrosine-protein phosphatase non-receptor type 3 Protein-tyrosine phosphatase H1 PTPN3 PTPH1 protein protein human Homo sapiens Human MTSRLRALGGRINNIRTSELPKEKTRSEVICSIHFLDGVVQTFKVTKQDTGQVLLDMVHNHLGVTEKEYFGLQHDDDSVDSPRWLEASKAIRKQLKGGFPCTLHFRVRFFIPDPNTLQQEQTRHLYFLQLKMDICEGRLTCPLNSAVVLASYAVQSHFGDYNSSIHHPGYLSDSHFIPDQNEDFLTKVESLHEQHSGLKQSEAESCYINIARTLDFYGVELHSGRDLHNLDLMIGIASAGVAVYRKYICTSFYPWVNILKISFKRKKFFIHQRQKQAESREHIVAFNMLNYRSCKNLWKSCVEHHTFFQAKKLLPQEKNVLSQYWTMGSRNTKKSVNNQYCKKVIGGMVWNPAMRRSLSVEHLETKSLPSRSPPITPNWRSPRLRHEIRKPRHSSADNLANEMTYITETEDVFYTYKGSLAPQDSDSEVSQNRSPHQESLSENNPAQSYLTQKSSSSVSPSSNAPGSCSPDGVDQQLLDDFHRVTKGGSTEDASQYYCDKNDNGDSYLVLIRITPDEDGKFGFNLKGGVDQKMPLVVSRINPESPADTCIPKLNEGDQIVLINGRDISEHTHDQVVMFIKASRESHSRELALVIRRRAVRSFADFKSEDELNQLFPEAIFPMCPEGGDTLEGSMAQLKKGLESGTVLIQFEQLYRKKPGLAITFAKLPQNLDKNRYKDVLPYDTTRVLLQGNEDYINASYVNMEIPAANLVNKYIATQGPLPHTCAQFWQVVWDQKLSLIVMLTTLTERGRTKCHQYWPDPPDVMNHGGFHIQCQSEDCTIAYVSREMLVTNTQTGEEHTVTHLQYVAWPDHGVPDDSSDFLEFVNYVRSLRVDSEPVLVHCSAGIGRTGVLVTMETAMCLTERNLPIYPLDIVRKMRDQRAMMVQTSSQYKFVCEAILRVYEEGLVQMLDPS DIP protein P26045 original sequence version: 155 DIP protein P26045-1 original sequence version: 152 44FBBFA35A5F2AFF tera_mouse Transitional endoplasmic reticulum ATPase Vcp 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein mouse Mus musculus Mouse MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein Q01853 original sequence version: 84 DIP protein Q01853 original sequence version: 112 DIP protein Q01853 original sequence version: 152 DIP protein Q01853 original sequence version: 136 DIP protein Q01853 original sequence version: 156 DIP protein Q01853 original sequence version: 149 501B721D3A77BA8A ptpn3-vcp 3 PTPH1 5 unspecified role unspecified role bait bait purified purified gst-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined catalytic region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P26045:p.Asp811Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:29:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp811ala' Tue Jun 30 17:40:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp811Ala' ecolx Escherichia coli VCP 4 unspecified role unspecified role prey prey cell lysate cell lysate tyrosine phosphorylation modtyr modified L-tyrosine residue ModTyr monoclonal western monoclonal antibody western blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Fig 3 ptpn3-vcp-1 2 PTPH1 5 unspecified role unspecified role bait bait catalytic region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ha-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P26045:p.[Tyr676Phe;Asp811Ala] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:32:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr676phe,asp811ala' Tue Jun 30 19:07:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr676Phe;Asp811Ala]' Tyr676Phe VCP 4 unspecified role unspecified role prey prey tyrosine phosphorylation modtyr modified L-tyrosine residue ModTyr monoclonal western monoclonal antibody western blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4B ptpn3-vcp-2 1 PTPH1 5 unspecified role unspecified role bait bait ha-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P26045:p.[Tyr676Phe;Asp811Ala] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:00:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr676phe,asp811ala' Tue Jun 30 19:33:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr676Phe;Asp811Ala]' Tyr676Phe catalytic region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain VCP 6 unspecified role unspecified role prey prey Q01853:p.[Tyr796Phe;Tyr805Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 17:29:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr796phe,tyr805phe' Tue Jun 30 18:13:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr796Phe;Tyr805Phe]' myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5