IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Oncogene (0950-9232) 1998 imex curation Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. A murine embryonic E11.5 cDNA library (Clonetech) was used. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. Accepted 2007-APR-7 by JYOTI. A murine embryonic E11.5 cDNA library (Clonetech) was used. Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Oncogene (0950-9232) 1998 imex curation Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. A murine embryonic E11.5 cDNA library (Clonetech) was used. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. Accepted 2007-APR-7 by JYOTI. Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Oncogene (0950-9232) 1998 imex curation Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. A murine embryonic E11.5 cDNA library (Clonetech) was used. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given The sequence of Pax-3 corresponding to the first two helices of the paired-like homoedomain was used to determine the range shown in EBI-1208574. A unique UniProtKb entry could not be associated with PHox since the organism and subtype of this interactor cannot be ascertained. It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. Accepted 2007-APR-7 by JYOTI. Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Oncogene (0950-9232) 1998 imex curation Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. A murine embryonic E11.5 cDNA library (Clonetech) was used. Only protein-protein interactions human-c33a Homo sapiens epihelial cervix carcinoma cells c33a C33A cells epithelial-like cells, grown as monolayer; derived from cervical carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given CMV-Pax-3-HA was donated by J Epstein and the papers with by this author (Cancer Res 58 4426, JBC 270 11729) confirm that this constuct contains murine Pax-3. It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. Accepted 2007-APR-7 by JYOTI. Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Interaction of the pRB-family proteins with factors containing paired-like homeodomains. Oncogene (0950-9232) 1998 imex curation Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given It was not possible to find a UniProtKb entry corresponding to the novel protein referred to as B4 by the authors. A murine embryonic E11.5 cDNA library (Clonetech) was used. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Wiggan O., Taniguchi-Sidle A., Hamel PA. Cancer - Interactions investigated in the context of cancer No contact email address given A unique UniProtKb entry could not be associated with PHox since the organism and subtype of this interactor cannot be ascertained. Accepted 2007-APR-7 by JYOTI. Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation rb1_cerae_protein Cercopithecus aethiops retinoblastoma protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt rb_mouse Retinoblastoma-associated protein Rb1 Rb-1 pRb pp105 p110-RB1 protein protein mouse Mus musculus Mouse MPPKAPRRAAAAEPPPPPPPPPREDDPAQDSGPEELPLARLEFEEIEEPEFIALCQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNAMSRLLKKYNVLCALYSKLERTCELIYLTQPSSALSTEINSMLVLKISWITFLLAKGEVLQMEDDLVISFQLMLCVVDYFIKFSPPALLREPYKTAAIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFINSLGIVSSNGLPEVESLSKRYEEVYLKNKDLDARLFLDHDKTLQTDPIDSFETERTPRKNNPDEEANVVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFNNCTVNPKENILKRVKDVGHIFKEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTLQHLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDNLEPACPLSLPLQGNHTAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLALFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGADEADGSKHLPAESKFQQKLAEMTSTRTRMQKQRMNESKDVSNKEEK DIP protein P13405 original sequence version: 115 DIP protein P13405 original sequence version: 114 DIP protein P13405 original sequence version: 110 AE81D35A07ADB493 rbl2_human Retinoblastoma-like protein 2 RBL2 RB2 Retinoblastoma-related protein 2 130 kDa retinoblastoma-associated protein pRb2 protein protein human Homo sapiens Human MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVSTATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFFRKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSH DIP protein Q08999 original sequence version: 144 DIP protein Q08999 original sequence version: 107 D129032FB1F383D4 rbl1_human Retinoblastoma-like protein 1 RBL1 pRb1 107 kDa retinoblastoma-associated protein protein protein human Homo sapiens Human MFEDKPHAEGAAVVAAAGEALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVTHWLACSLYVACRKSIIPTVGKGIMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKYEPIFLDIFQNPYEEPPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIFANAIMCPNRQDLLNPSFKGLPSDFHTADFTASEEPPCIIAVLCELHDGLLVEAKGIKEHYFKPYISKLFDRKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFTRDTPLGKLTAQANVEYNLQQHFEKKRSFAPSTPLTGRRYLREKEAVITPVASATQSVSRLQSIVAGLKNAPSDQLINIFESCVRNPVENIMKILKGIGETFCQHYTQSTDEQPGSHIDFAVNRLKLAEILYYKILETVMVQETRRLHGMDMSVLLEQDIFHRSLMACCLEIVLFAYSSPRTFPWIIEVLNLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEEQILESLAWSHDSALWEALQVSANKVPTCEEVIFPNNFETGNGGNVQGHLPLMPMSPLMHPRVKEVRTDSGSLRRDMQPLSPISVHERYSSPTAGSAKRRLFGEDPPKEMLMDKIITEGTKLKIAPSSSITAENVSILPGQTLLTMATAPVTGTTGHKVTIPLHGVANDAGEITLIPLSMNTNQESKVKSPVSLTAHSLIGASPKQTNLTKAQEVHSTGINRPKRTGSLALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHLDQLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIPREVVAYNKNINDDFEMIDCDLEDATKTPDCSSGPVKEERGDLIKFYNTIYVGRVKSFALKYDLANQDHMMDAPPLSPFPHIKQQPGSPRRISQQHSIYISPHKNGSGLTPRSALLYKFNGSPSKSLKDINNMIRQGEQRTKKRVIAIDSDAESPAKRVCQENDDVLLKRLQDVVSERANH DIP protein P28749 original sequence version: 154 2BC4876EA04BDB31 prrx1_mouse Paired mesoderm homeobox protein 1 Prrx1 Pmx Pmx1 Paired-related homeobox protein 1 Homeobox protein mHox Homeobox protein K-2 protein protein mouse Mus musculus Mouse MTSSYGHVLERQPALGGRLDSPGNLDTLQAKKNFSVSHLLDLEEAGDMVAAQADESVGEAGRSLLESPGLTSGSDTPQQDNDQLNSEEKKKRKQRRNRTTFNSSQLQALERVFERTHYPDAFVREDLARRVNLTEARVQVWFQNRRAKFRRNERAMLANKNASLLKSYSGDVTAVEQPIVPRPAPRPTDYLSWGTASPYSAMATYSATCANNSPAQGINMANSIANLRLKAKEYSLQRNQVPTVN FF867F0D856E1115 vsx2_mouse Visual system homeobox 2 Vsx2 Chx10 Homeobox protein CHX10 Ceh-10 homeodomain-containing homolog protein protein mouse Mus musculus Mouse MTGKAGEALSKPKSETVAKSTSGGAPARCTGFGIQEILGLNKEPPSSHPRAALDGLAPGHLLAARSVLSPAGVGSMGLLGPGGLPGFYTQPTFLEVLSDPQSVHLQPLGRASGPLDTSQTASSDSEDVSSSDRKMSKSALNQTKKRKKRRHRTIFTSYQLEELEKAFNEAHYPDVYAREMLAMKTELPEDRIQVWFQNRRAKWRKREKCWGRSSVMAEYGLYGAMVRHSIPLPESILKSAKDGIMDSCAPWLLGMHKKSLEAAAESGRKPEVERQALPKLDKMEQEERAPEAQAAISQEELRENSIAALRAKAQEHSTKVLGTVSGPDSLARNAEKPEEEDATEEDRPAEKLSPPQLEDMA 2DB7FF7F8C7BE0AA pax3_mouse Paired box protein Pax-3 Pax3 Pax-3 protein protein mouse Mus musculus Mouse MTTLAGAVPRMMRPGPGQNYPRSGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPKQVTTPDVEKKIEEYKRENPGMFSWEIRDKLLKDAVCDRNTVPSVSSISRILRSKFGKGEEEEADLERKEAEESEKKAKHSIDGILSERASAPQSDEGSDIDSEPDLPLKRKQRRSRTTFTAEQLEELERAFERTHYPDIYTREELAQRAKLTEARVQVWFSNRRARWRKQAGANQLMAFNHLIPGGFPPTAMPTLPTYQLSETSYQPTSIPQAVSDPSSTVHRPQPLPPSTVHQSTIPSNADSSSAYCLPSTRHGFSSYTDSFVPPSGPSNPMNPTIGNGLSPQVMGLLTNHGGVPHQPQTDYALSPLTGGLEPTTTVSASCSQRLEHMKNVDSLPTSQPYCPPTYSTAGYSMDPVTGYQYGQYGQSKPWTF 8806E551FFB755A0 rbl2-pax3 1 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Eight cDNAs encoding proteins that specifically interacted with the N-terminus of p130 were isolated in the two-hybrid assay. chx10-pax3 1 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Eight cDNAs encoding proteins that specifically interacted with the N-terminus of p130 were isolated in the two-hybrid assay. pax3-rb1 3 7 unspecified role unspecified role prey prey mouse-3t7 Mus musculus 3T7 T cell lymphoma cell line 3t7 mouse 3T7 T cell lymphoma cell line 12 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1 B pRB (Figure 1b), p107 (Figure 1c) and p130 (Figure 1d) specifically bound to Pax-3, B4 and Chx10 but did not bind to beads containing lysates from bacteria expressing GST alone or the empty His-tagged vector, pRSET. The range for the shortest construct of Pax-3 that bound to pRb was not given but from the diagram in 1 A, it appears the construct starts approximately at the start of the paired domain. The UniProtKb entry shows the paired domain to begin at residue 34. chx10-rb1 3 7 unspecified role unspecified role prey prey mouse-3t7 Mus musculus 3T7 T cell lymphoma cell line 3t7 mouse 3T7 T cell lymphoma cell line 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 B pRB (Figure 1b), p107 (Figure 1c) and p130 (Figure 1d) specifically bound to Pax-3, B4 and Chx10 but did not bind to beads containing lysates from bacteria expressing GST alone or the empty His-tagged vector, pRSET. chx10-rbl1 3 9 unspecified role unspecified role prey prey human-c33a Homo sapiens epihelial cervix carcinoma cells c33a C33A cells epithelial-like cells, grown as monolayer; derived from cervical carcinoma 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1 C pRB (Figure 1b), p107 (Figure 1c) and p130 (Figure 1d) specifically bound to Pax-3, B4 and Chx10 but did not bind to beads containing lysates from bacteria expressing GST alone or the empty His-tagged vector, pRSET. pax3-rbl1 3 9 unspecified role unspecified role prey prey human-c33a Homo sapiens epihelial cervix carcinoma cells c33a C33A cells epithelial-like cells, grown as monolayer; derived from cervical carcinoma 12 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1 C pRB (Figure 1b), p107 (Figure 1c) and p130 (Figure 1d) specifically bound to Pax-3, B4 and Chx10 but did not bind to beads containing lysates from bacteria expressing GST alone or the empty His-tagged vector, pRSET. pax3-rbl2 3 8 unspecified role unspecified role prey prey human-c33a Homo sapiens epihelial cervix carcinoma cells c33a C33A cells epithelial-like cells, grown as monolayer; derived from cervical carcinoma 12 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1 D pRB (Figure 1b), p107 (Figure 1c) and p130 (Figure 1d) specifically bound to Pax-3, B4 and Chx10 but did not bind to beads containing lysates from bacteria expressing GST alone or the empty His-tagged vector, pRSET. The range for the shortest construct of Pax-3 that bound to pRb was not given but from the diagram in 1 A, it appears the construct starts approximately at the start of the paired domain. The UniProtKb entry shows the paired domain to begin at residue 34. chx10-rbl2 3 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey physical association physical association 1 D pRB (Figure 1b), p107 (Figure 1c) and p130 (Figure 1d) specifically bound to Pax-3, B4 and Chx10 but did not bind to beads containing lysates from bacteria expressing GST alone or the empty His-tagged vector, pRSET. chx10-rbl2-1 3 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1 D pRB (Figure 1b), p107 (Figure 1c) and p130 (Figure 1d) specifically bound to Pax-3, B4 and Chx10 but did not bind to beads containing lysates from bacteria expressing GST alone or the empty His-tagged vector, pRSET. rb1-pax3 2 12 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 Pax-3 bound strongly to GST-pRB rb1-chx10 2 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 2 Chx10 bound strongly to GST-p130 although weaker binding to GST-pRB was seen for this paired-like homeodomain protein in this assay Binding by Chx10 to GST-pRb was weaker than to GST-p130. rbl2-chx10 2 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 Chx10 bound strongly to GST-p130 although weaker binding to GST-pRB was seen for this paired-like homeodomain protein in this assay Binding by Chx10 to GST-pRb was weaker than to GST-p130. rbl1-pax3 4 9 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 Endogenous p107 or p130 was immunoprecipitated from C33A cells programmed to stably express an HA-tagged version of Pax-3. As Figure 3 clearly demonstrates, Pax-3 forms stable complexes with both of these pRB-family proteins in vivo. rbl2-pax3-1 4 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 Endogenous p107 or p130 was immunoprecipitated from C33A cells programmed to stably express an HA-tagged version of Pax-3. As Figure 3 clearly demonstrates, Pax-3 forms stable complexes with both of these pRB-family proteins in vivo. rbl2-prrx1 1 8 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey physical association physical association one cDNA encoded the mouse paired-like homeodomain protein, MHox chx10-rb1-1 3 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 1 B When lysates were passed over columns containing GST-Chx10, this fusion protein clearly bound to the unphosphorylated pRb present in the l-phosphatase treated (P) lysates. However, GST-Chx10 also bound exclusively to the unphosphorylated form of pRB in untreated lysates (L) and does not pull down the hyperphosphorylated species The unphosphorylated form of pRb binds to Chx10 while the hyperphosphorylated form of pRB shows little affinity for Chx10. The authors specify COS cells were used but do not give a subtype of this cell line. rb1-pax3-1 5 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-c33a Homo sapiens epihelial cervix carcinoma cells c33a C33A cells epithelial-like cells, grown as monolayer; derived from cervical carcinoma physical association physical association 5 B The resutls indicate that the association between Pax-3 and pRb involves multiple contacts. pax3-rbl1-1 3 9 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range human-c33a Homo sapiens epihelial cervix carcinoma cells c33a C33A cells epithelial-like cells, grown as monolayer; derived from cervical carcinoma 12 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 6 Deletion of the helix III (Pax-3DH3) did not a€ect the ability of Pax-3 to bind to p107 The removal of all three helices of the paired-like homeodomain of Pax-3 abrogated binding to p107.