IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural and functional consequences of the STAT5BN642H driver mutation. Structural and functional consequences of the STAT5BN642H driver mutation. Nature communications 2019 imex curation de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Only protein-protein interactions imex curation in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer Kd are provided for pH 7.25 no kd was provided for Fig. S4 as there's no agreement between the the kd provided in figure (expressed as microMolar) and the one mentioned in text (expressed as nanoMolar) patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Accepted 2020-MAY-12 AT 08:34 BST AT 08:34 BST by KALPANAP Nature communications 2019 Only protein-protein interactions imex curation imex curation Structural and functional consequences of the STAT5BN642H driver mutation. Structural and functional consequences of the STAT5BN642H driver mutation. Nature communications 2019 imex curation de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay weight by staining molecular weight estimation by staining weight by staining de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer from ref. 33 (PMID: 30223143) Abl is from human origin. patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Accepted 2020-MAY-12 AT 08:34 BST AT 08:34 BST by KALPANAP Nature communications 2019 Only protein-protein interactions imex curation imex curation Structural and functional consequences of the STAT5BN642H driver mutation. Structural and functional consequences of the STAT5BN642H driver mutation. Nature communications 2019 imex curation de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Only protein-protein interactions imex curation in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Accepted 2020-MAY-12 AT 08:34 BST AT 08:34 BST by KALPANAP Nature communications 2019 Only protein-protein interactions imex curation imex curation Structural and functional consequences of the STAT5BN642H driver mutation. Structural and functional consequences of the STAT5BN642H driver mutation. Nature communications 2019 imex curation de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Accepted 2020-MAY-12 AT 08:34 BST AT 08:34 BST by KALPANAP Nature communications 2019 Only protein-protein interactions imex curation imex curation Structural and functional consequences of the STAT5BN642H driver mutation. Structural and functional consequences of the STAT5BN642H driver mutation. Nature communications 2019 imex curation de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer from ref. 33 (PMID: 30223143) Abl is from human origin. patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Accepted 2020-MAY-12 AT 08:34 BST AT 08:34 BST by KALPANAP Nature communications 2019 Only protein-protein interactions imex curation imex curation Structural and functional consequences of the STAT5BN642H driver mutation. Structural and functional consequences of the STAT5BN642H driver mutation. Nature communications 2019 imex curation de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT., de Araujo ED., Erdogan F., Neubauer HA., Meneksedag-Erol D., Manaswiyoungkul P., Eram MS., Seo HS., Qadree AK., Israelian J., Orlova A., Suske T., Pham HTT., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown PJ., Audette GF., Rauscher S., Dhe-Paganon S., Moriggl R., Gunning PT. Cancer - Interactions investigated in the context of cancer patrick.gunning@utoronto.ca,richard.moriggl@vetmeduni.ac.at,dhepag@crystal.harvard.edu Accepted 2020-MAY-12 AT 08:34 BST AT 08:34 BST by KALPANAP Nature communications 2019 Only protein-protein interactions imex curation imex curation sta5b_human Signal transducer and activator of transcription 5B STAT5B protein protein human Homo sapiens Human MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLSQWIESQAWDSVDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFGPLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFESQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS AA2F1CAB20955ACA abl1_human Tyrosine-protein kinase ABL1 Abelson tyrosine-protein kinase 1 ABL1 ABL JTK7 Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 protein protein human Homo sapiens Human MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR DIP protein P00519 original sequence version: 114 DIP protein P00519 original sequence version: 137 DIP protein P00519 original sequence version: 161 DIP protein P00519 original sequence version: 220 DIP protein P00519 original sequence version: 219 85FE6C1C0E483EA2 stat5-bp STAT5 binding peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro GYLVLDKW stat5b-1 6 7 unspecified role unspecified role neutral component neutral component purified purified range sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5, Table S1 3.29A resolution R work: 0.266 R free: 0.305 stat5b-5 6 7 unspecified role unspecified role neutral component neutral component purified purified range sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51692:p.Asn642His mutation mutation certain certain sequence position certain certain certain sequence position certain N H Tue Jun 30 19:47:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642His' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5, Table S1 3.21A resolution R work: 0.265 R free: 0.287 stat5b-6 1 9 unspecified role unspecified role neutral component neutral component purified purified phosphorylated Tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm FAM label fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified P51692:p.Asn642His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N H Tue Jun 30 20:24:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642His' P51692:p.Asn642Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 17:49:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642Ala' P51692:p.Asn642Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N D Tue Jun 30 19:27:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642Asp' P51692:p.Asn642Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Tue Jun 30 18:23:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642Gln' P51692:p.Asn642Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N E Tue Jun 30 16:25:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 6A stat5_bp-stat5b-1 1 9 unspecified role unspecified role neutral component neutral component purified purified phosphorylated Tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm FAM label fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified range sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51692:p.Asn642His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N H Tue Jun 30 20:26:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642His' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. S4 stat5_bp-stat5b-2 5 8 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey purified purified P51692:p.Asn642His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N H Tue Jun 30 18:57:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642His' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 8A stat5b-7 3 7 unspecified role unspecified role neutral component neutral component purified purified P51692:p.Asn642His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N H Tue Jun 30 18:06:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642His' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 7E Measurements of the particle size distribution of STAT5B via DLS suggest the full-length protein exists predominantly as a dimer (d = 21.42 ± 0.29 nm), which is also supported by gel filtration experiments (Supplementary Fig. 5). Similar experiments with STAT5BN642H yielded a slightly smaller average particle size (d = 18.89 ± 0.12 nm), suggesting a more compact or stable anti-parallel dimer stat5b-8 4 7 unspecified role unspecified role neutral component neutral component purified purified P51692:p.Asn642His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N H Tue Jun 30 18:20:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642His' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. S5 Measurements of the particle size distribution of STAT5B via DLS suggest the full-length protein exists predominantly as a dimer (d = 21.42 ± 0.29 nm), which is also supported by gel filtration experiments (Supplementary Fig. 5) abl1-stat5b-1 2 8 enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 enzyme target enzyme target substrate substrate neutral component neutral component purified purified P51692:p.Asn642His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N H Tue Jun 30 19:13:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn642His' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. S7