IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ N-Myc Induces an EZH2-Mediated Transcriptional Program Driving Neuroendocrine Prostate Cancer. N-Myc Induces an EZH2-Mediated Transcriptional Program Driving Neuroendocrine Prostate Cancer. Cancer cell 2016 imex curation Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu Accepted 2017-JAN-27 AT 13:43 GMT AT 13:43 GMT by ORCHARD Cancer cell 2016 imex curation N-Myc Induces an EZH2-Mediated Transcriptional Program Driving Neuroendocrine Prostate Cancer. N-Myc Induces an EZH2-Mediated Transcriptional Program Driving Neuroendocrine Prostate Cancer. Cancer cell 2016 imex curation Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu Accepted 2017-JAN-27 AT 13:43 GMT AT 13:43 GMT by ORCHARD Cancer cell 2016 imex curation N-Myc Induces an EZH2-Mediated Transcriptional Program Driving Neuroendocrine Prostate Cancer. N-Myc Induces an EZH2-Mediated Transcriptional Program Driving Neuroendocrine Prostate Cancer. Cancer cell 2016 imex curation Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu Accepted 2017-JAN-27 AT 13:43 GMT AT 13:43 GMT by ORCHARD Cancer cell 2016 imex curation max_human Protein max MAX BHLHD4 Class D basic helix-loop-helix protein 4 Myc-associated factor X protein protein human Homo sapiens Human MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS DIP protein P61244 original sequence version: 132 DIP protein P61244 original sequence version: 128 DIP protein P61244 original sequence version: 150 DIP protein P61244 original sequence version: 120 EB10F3137727A56F fkbp5_human_gene Human FKBP5 gene gene gene human Homo sapiens Human aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS DIP protein O14965 original sequence version: 181 DIP protein O14965 original sequence version: 146 DIP protein O14965 original sequence version: 124 DIP protein O14965 original sequence version: 160 DIP protein O14965 original sequence version: 138 DIP protein O14965 original sequence version: 98 125F3594834CD157 ezh2_human Histone-lysine N-methyltransferase EZH2 KMT6 Enhancer of zeste homolog 2 ENX-1 Lysine N-methyltransferase 6 EZH2 protein protein human Homo sapiens Human MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP DIP protein Q15910 original sequence version: 97 DIP protein Q15910 original sequence version: 94 DIP protein Q15910 original sequence version: 92 DIP protein Q15910 original sequence version: 96 DIP protein Q15910 original sequence version: 122 DIP protein Q15910 original sequence version: 100 1B5029EB9D509BE5 klk3_human_gene Human kallikrein-related peptidase 3 (KLK3) (PSA) gene gene human Homo sapiens Human cadh5_human_gene Cadherin-5 CDH5 gene gene human Homo sapiens Human suz12_human Polycomb protein SUZ12 CHET9 JJAZ1 KIAA0160 SUZ12 Suppressor of zeste 12 protein homolog Joined to JAZF1 protein Chromatin precipitated E2F target 9 protein protein protein human Homo sapiens Human MAPQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL DIP protein Q15022 original sequence version: 92 DIP protein Q15022 original sequence version: 88 DIP protein Q15022 original sequence version: 91 DIP protein Q15022 original sequence version: 106 A8830EBC3FD38D56 mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC DIP protein P04198 original sequence version: 157 560E885602E30DAD tmps2_human_gene Human TMPS2/TMPRSS2 gene gene gene human Homo sapiens Human andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ DIP protein P10275 original sequence version: 220 DIP protein P10275-1 original sequence version: 213 DIP protein P10275 original sequence version: 216 DIP protein P10275 original sequence version: 143 DIP protein P10275 original sequence version: 174 DIP protein P10275 original sequence version: 169 DIP protein P10275 original sequence version: 188 DIP protein P10275-1 original sequence version: 188 DIP protein P10275 original sequence version: 196 DIP protein P10275 original sequence version: 217 DIP protein P10275-1 original sequence version: 217 DIP protein P10275-1 original sequence version: 207 A73432C55D39AE06 sox17_human_gene Transcription factor SOX-17 SOX17 gene gene human Homo sapiens Human ndka_human_gene Nucleoside diphosphate kinase A NME1 NDPKA NM23 gene gene human Homo sapiens Human ndrg1_human_gene Human NDRG1 gene gene gene human Homo sapiens Human d8ct6yud5-1 3 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association figure 4D- small inset image under "KLK3 Locus" mycn-klk3_human_gene-1 1 8 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association figure 4D CHEBI:16330 increases the binding of P04198 to ENSG00000142515 DHT: CHEBI:16330 mycn-tmps2_human_gene-1 1 12 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association figure 4Di CHEBI:16330 increases the binding of P04198 to ENSG00000184012 DHT: CHEBI:16330 mycn-tmps2_human_gene-2 1 16 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association figure 4Dii CHEBI:16330 increases the binding of P04198 to ENSG00000104419 DHT: CHEBI:16330 mycn-ar-1 3 7 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey association association 7B-1 An EZH2 inhibitor GSK126 reduces the interaction with N-Myc and AR (but not SUZ12) An EZH2 inhibitor GSK343 reduces the interaction with N-Myc and AR (but not SUZ12) ezh2-ar-1 3 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 7B-2 An EZH2 inhibitor GSK126 reduces the interaction with N-Myc and AR (but not SUZ12 or MAX) An EZH2 inhibitor GSK343 reduces the interaction with N-Myc and AR (but not SUZ12 or MAX) mycn-ar-2 3 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 7B-3 (Supp7C) An EZH2 mutant of unknown origin, lacking the SET domain sequence (aas 612-727 in the Human protein) still interacted. the figure legends appear to be muddled for these Figs - I have no idea what the minus sign over Supp Fig 7C refers to-probably without the mutant, but why is WT not detected - it is elsewhere -7B. mycn-ar-3 3 4 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 7B-4 In the presence of EZH2 siRNA, only MAX was detected. asn235lys-1 1 8 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait association association 7D-1 N-myc over-expression ezh2-klk3_human_gene-1 1 14 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association 7D-2 N-myc over-expression ezh2-klk3_human_gene-2 1 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait association association 7D-3 ezh2-klk3_human_gene-3 1 5 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait association association 7D-4 N-myc over-expression ezh2-fkbp5_human_gene-1 1 12 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait association association 7D-5 N-myc over-expression ezh2-tmps2_human_gene-1 1 16 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association 7D-6 N-myc over-expression r9rd9-1 2 6 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 8A MLN8237;an Allosteric Aurora-A Inhibitor mycn-max-3 3 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 8B MLN8237:An allosteric Aurora-A Inhibitor reduced the interaction of N-Myc with Aurora A but not MAX. mycn-tmps2_human_gene-3 1 9 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association Supp fig 4E CHEBI:16330 increases the binding of P04198 to ENSG00000184012 DHT: CHEBI:16330 ezh2-klk3_human_gene-4 1 8 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait association association Supp 4D N-myc over-expression (small effect) ezh2-tmps2_human_gene-2 1 12 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait association association Supp 4D-2 N-myc over-expression (slight effect) ezh2-ndrg1_human_gene-1 1 16 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait association association Supp 4D-3 N-myc over-expression