IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Cancer cell 2016 imex curation Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer The phosphorylation of ACC1 by AMPK has not been entered as the origins and identity of AMPK has not been stated. email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Accepted 2017-FEB-07 AT 08:50 GMT AT 08:50 GMT by ORCHARD Cancer cell 2016 Only protein-protein interactions imex curation imex curation I have moved Fig 6A to accepted Expt-2 - no idea why it was here.; This must be In Vitro - fig 4A states -" Equal amounts of lysate (by protein mass) from each (SILAC) cell line are separately incubated with a “bulky” ATPγS analog..." I dont understand what you meant about the auto-phosp. validity -presumably you mean interaction EBI-12562539 - but if one result is OK - why not the other? the text states that "SIK2 S358 was indeed an autophosphorylation site".; I have moved figure 7B results to accepted Expt-2 - but I cannot see these results entered anywhere else.; altered as suggested Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Cancer cell 2016 imex curation Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Only protein-protein interactions imex curation in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. Lipid Kinase assay using PI3 Kinase Activity/ Inhibitor Assay Kit (Millipore) -ELISA. Phosphatidylinositol 3,4-bisphosphate (PIP2) is phosphorylated by p110alpha to PIP3. GST tagged p85a/GRP1 is bound to the glutathione-plate and binds PIP3 formed. A PIP3-biotin tracer is included in the kit. The amount of PIP3-Biotin is detected at the end of the assay using a streptavidin-HRP conjugate and a colorimetric read out. The colour shown is inversely proportional to kinase activity. ahmed.ahmed@obs-gyn.ox.ac.uk Accepted 2017-FEB-07 AT 08:50 GMT AT 08:50 GMT by ORCHARD Cancer cell 2016 Only protein-protein interactions imex curation imex curation I have moved Fig 6A to accepted Expt-2 - no idea why it was here.; This must be In Vitro - fig 4A states -" Equal amounts of lysate (by protein mass) from each (SILAC) cell line are separately incubated with a “bulky” ATPγS analog..." I dont understand what you meant about the auto-phosp. validity -presumably you mean interaction EBI-12562539 - but if one result is OK - why not the other? the text states that "SIK2 S358 was indeed an autophosphorylation site".; I have moved figure 7B results to accepted Expt-2 - but I cannot see these results entered anywhere else.; altered as suggested Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Cancer cell 2016 imex curation Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Accepted 2017-FEB-07 AT 08:50 GMT AT 08:50 GMT by ORCHARD Cancer cell 2016 Only protein-protein interactions imex curation imex curation I have moved Fig 6A to accepted Expt-2 - no idea why it was here.; This must be In Vitro - fig 4A states -" Equal amounts of lysate (by protein mass) from each (SILAC) cell line are separately incubated with a “bulky” ATPγS analog..." I dont understand what you meant about the auto-phosp. validity -presumably you mean interaction EBI-12562539 - but if one result is OK - why not the other? the text states that "SIK2 S358 was indeed an autophosphorylation site".; I have moved figure 7B results to accepted Expt-2 - but I cannot see these results entered anywhere else.; altered as suggested Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Cancer cell 2016 imex curation Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Accepted 2017-FEB-07 AT 08:50 GMT AT 08:50 GMT by ORCHARD Cancer cell 2016 Only protein-protein interactions imex curation imex curation I have moved Fig 6A to accepted Expt-2 - no idea why it was here.; This must be In Vitro - fig 4A states -" Equal amounts of lysate (by protein mass) from each (SILAC) cell line are separately incubated with a “bulky” ATPγS analog..." I dont understand what you meant about the auto-phosp. validity -presumably you mean interaction EBI-12562539 - but if one result is OK - why not the other? the text states that "SIK2 S358 was indeed an autophosphorylation site".; I have moved figure 7B results to accepted Expt-2 - but I cannot see these results entered anywhere else.; altered as suggested Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Cancer cell 2016 imex curation Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. SILAC-labelled SKOv3-SIK2 cells were used. A mutation (T96G) was introduced into SIK2 which allows the kinase to utilize “bulky” N6-substituted ATP analogs (ATPγS in this case), which cannot be accepted by wild-type kinases. Substrate proteins of the mutant kinase are thus specifically thiophosphorylated and can be isolated by "Covalent Capture" - affinity purification using an Iodoacetyl resin that binds free SH groups. ahmed.ahmed@obs-gyn.ox.ac.uk Accepted 2017-FEB-07 AT 08:50 GMT AT 08:50 GMT by ORCHARD Cancer cell 2016 Only protein-protein interactions imex curation imex curation I have moved Fig 6A to accepted Expt-2 - no idea why it was here.; This must be In Vitro - fig 4A states -" Equal amounts of lysate (by protein mass) from each (SILAC) cell line are separately incubated with a “bulky” ATPγS analog..." I dont understand what you meant about the auto-phosp. validity -presumably you mean interaction EBI-12562539 - but if one result is OK - why not the other? the text states that "SIK2 S358 was indeed an autophosphorylation site".; I have moved figure 7B results to accepted Expt-2 - but I cannot see these results entered anywhere else.; altered as suggested Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Cancer cell 2016 imex curation Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ahmed.ahmed@obs-gyn.ox.ac.uk Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay antibody detection identification by antibody antibody detection Miranda F., Mannion D., Liu S., Zheng Y., Mangala LS., Redondo C., Herrero-Gonzalez S., Xu R., Taylor C., Chedom DF., Karaminejadranjbar M., Albukhari A., Jiang D., Pradeep S., Rodriguez-Aguayo C., Lopez-Berestein G., Salah E., Abdul Azeez KR., Elkins JM., Campo L., Myers KA., Klotz D., Bivona S., Dhar S., Bast RC., Saya H., Choi HG., Gray NS., Fischer R., Kessler BM., Yau C., Sood AK., Motohara T., Knapp S., Ahmed AA. Cancer - Interactions investigated in the context of cancer email sent 26/10:no reply.. ELISA format using antibodies for detecting phosphorylation of S154. ahmed.ahmed@obs-gyn.ox.ac.uk Accepted 2017-FEB-07 AT 08:50 GMT AT 08:50 GMT by ORCHARD Cancer cell 2016 Only protein-protein interactions imex curation imex curation I have moved Fig 6A to accepted Expt-2 - no idea why it was here.; This must be In Vitro - fig 4A states -" Equal amounts of lysate (by protein mass) from each (SILAC) cell line are separately incubated with a “bulky” ATPγS analog..." I dont understand what you meant about the auto-phosp. validity -presumably you mean interaction EBI-12562539 - but if one result is OK - why not the other? the text states that "SIK2 S358 was indeed an autophosphorylation site".; I have moved figure 7B results to accepted Expt-2 - but I cannot see these results entered anywhere else.; altered as suggested pk3ca_human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Phosphoinositide-3-kinase catalytic alpha polypeptide Serine/threonine protein kinase PIK3CA PIK3CA Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha Phosphoinositide 3-kinase alpha protein protein human Homo sapiens Human MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN DIP protein P42336 original sequence version: 110 DIP protein P42336 original sequence version: 119 DIP protein P42336 original sequence version: 166 DIP protein P42336 original sequence version: 139 041487231A9A1207 hg-9-91-01 HG-9-91-01 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 3,4-bisphosphate 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q13085-4 Acetyl-CoA carboxylase 1 ACACA ACAC ACC1 ACCA Acetyl-Coenzyme A carboxylase alpha protein protein human Homo sapiens Human MWWSTLMSILRARSFWKWISTQTVRIIRAVRAHFGGIMDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST 1F2B8F96208B9983 p85a_human Phosphatidylinositol 3-kinase regulatory subunit alpha PIK3R1 GRB1 Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha protein protein human Homo sapiens Human MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGSSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR DIP protein P27986 original sequence version: 181 DIP protein P27986 original sequence version: 184 DIP protein P27986-1 original sequence version: 135 DIP protein P27986 original sequence version: 135 DIP protein P27986-1 original sequence version: 145 DIP protein P27986-1 original sequence version: 147 DIP protein P27986 original sequence version: 191 B9DAD8416C33140F sik2_human Serine/threonine-protein kinase SIK2 SIK2 Qin-induced kinase SNF1LK2 KIAA0781 QIK Serine/threonine-protein kinase SNF1-like kinase 2 Salt-inducible kinase 2 protein protein human Homo sapiens Human MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN 9D7F9BF81FD65CCF bosutinib Bosutinib small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) Protein kinase inhibitor Protein kinase inhibitor sik2-1 1 12 putative self putative self putative self putative self ser90 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ser343 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ser358 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true 3 A/B sik2-3 3 12 putative self putative self putative self putative self n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ser358 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true 3C, Supp 3I lambda phosphatase HG-9-91-01 (CHEBI:133818) - a pan-SIK inhibitor CHEBI:133818 decreases the autophosphorylation of Q9H0K1 For Supplementary Figure 3I it is stated that PKA (P17612) also phosphorylates SIK2, but from the evidence shown it is not possible to distinguish this effect from SIK2 autophosphorylation . atp-sik2-1 5 12 putative self putative self putative self putative self thio-ser358 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain Q9H0K1:p.Thr96Gly mutation mutation certain certain sequence position certain certain certain sequence position certain T G Wed May 02 14:26:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr96gly' Tue Jun 30 17:00:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr96Gly' human-skov_3 Homo sapiens ovarian adenocarcinoma cell line skov_3 Human ovarian adenocarcinoma cell line Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells. phosphorylation phosphorylation reaction phosphorylation true 4E, Supp 4C pik3r1-sik2-1 5 12 enzyme enzyme neutral component neutral component ser358 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain Q9H0K1:p.Thr96Gly mutation mutation certain certain sequence position certain certain certain sequence position certain T G Wed May 02 16:53:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr96gly' Tue Jun 30 20:01:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr96Gly' human-skov_3 Homo sapiens ovarian adenocarcinoma cell line skov_3 Human ovarian adenocarcinoma cell line Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells. 11 enzyme target enzyme target substrate substrate neutral component neutral component possible_tags tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined thio-ser154 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 4E, Supp 4B acaca-sik2-1 1 10 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range thio-phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 6A "AMPK" alone phosphorylated ACC1 and augmented the phosphorylation of ACC1 in combination with SIK2. The identity and origin of "AMPK" is not stated. It has been assumed that details for the protein ACC1 (- supplied from Amsbio) are as entered, due to the fact this is the only protein with any details given on the Amsbio website. sik2-4 4 12 putative self putative self putative self putative self n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true 7A sik2-5 4 11 enzyme target enzyme target substrate substrate neutral component neutral component possible_tags tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 7Ai pik3r1-sik2-2 3 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 enzyme target enzyme target substrate substrate neutral component neutral component possible_tags tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined thio-phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 7B pik3r1-sik2-3 1 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 enzyme target enzyme target substrate substrate neutral component neutral component possible_tags tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser541 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ser154 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 7E pik3r1-sik2-4 3 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 enzyme target enzyme target substrate substrate neutral component neutral component possible_tags tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P27986:p.Ser541Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:41:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser541ala' Tue Jun 30 20:19:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser541Ala' ser154 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm P27986:p.Ser154Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:46:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser154ala' Tue Jun 30 18:23:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser154Ala' P27986:p.[Ser154Ala;Ser541Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:44:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser154ala,ser541ala' Tue Jun 30 17:43:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser154Ala;Ser541Ala]' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 7 F/G, Supp 7B Supplementary figure 7B also shows some increase in phosphorylation of p85a at Serine 541 in the presence of SIK2. Q9H0K1 activates P27986 q45aeyrdgf-1 2 7 enzyme enzyme neutral component neutral component 9 enzyme target enzyme target substrate substrate neutral component neutral component phosphorylation phosphorylation reaction phosphorylation 7M 1_phosphatidyl_1d_myo_inositol 3,4_bisphosphate-pik3ca-1 2 7 enzyme enzyme neutral component neutral component 9 enzyme target enzyme target substrate substrate neutral component neutral component 12 stimulator stimulator neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 7Mi Q9H0K1 activates P42336 Q9H0K1 increases activity of P42336 pik3r1-sik2-5 1 11 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain possible_tags tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosres_or_thio-phosres opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation Supp 7A I dont think Supp fig 7A shows which residue was phosph-ed pik3r1-sik2-6 6 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ser154 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm phosphorylation phosphorylation reaction phosphorylation Supp 7F pik3r1-sik2-7 6 13 inhibitor inhibitor neutral component neutral component 12 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ser154 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm phosphorylation phosphorylation reaction phosphorylation Supp 7Fi 1_phosphatidyl_1d_myo_inositol 3,4_bisphosphate-pik3ca-2 2 8 inhibitor inhibitor neutral component neutral component 7 enzyme enzyme neutral component neutral component 9 enzyme target enzyme target substrate substrate neutral component neutral component 12 stimulator stimulator neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation Supp 7G