IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Inhibiting Drivers of Non-mutational Drug Tolerance Is a Salvage Strategy for Targeted Melanoma Therapy. Inhibiting Drivers of Non-mutational Drug Tolerance Is a Salvage Strategy for Targeted Melanoma Therapy. Cancer cell 2016 imex curation Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C., Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C. Cancer - Interactions investigated in the context of cancer claudia.wellbrock@manchester.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-melanoma Human melanoma melanoma Melanoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C., Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C. Cancer - Interactions investigated in the context of cancer mutant BRAFV600-positive metastatic melanoma claudia.wellbrock@manchester.ac.uk Accepted 2017-AUG-09 AT 13:28 BST AT 13:28 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation Inhibiting Drivers of Non-mutational Drug Tolerance Is a Salvage Strategy for Targeted Melanoma Therapy. Inhibiting Drivers of Non-mutational Drug Tolerance Is a Salvage Strategy for Targeted Melanoma Therapy. Cancer cell 2016 imex curation Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C., Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C. Cancer - Interactions investigated in the context of cancer claudia.wellbrock@manchester.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C., Smith MP., Brunton H., Rowling EJ., Ferguson J., Arozarena I., Miskolczi Z., Lee JL., Girotti MR., Marais R., Levesque MP., Dummer R., Frederick DT., Flaherty KT., Cooper ZA., Wargo JA., Wellbrock C. Cancer - Interactions investigated in the context of cancer claudia.wellbrock@manchester.ac.uk Accepted 2017-AUG-09 AT 13:28 BST AT 13:28 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation smad2_human Mothers against decapentaplegic homolog 2 SMAD2 MADH2 MADR2 Mad-related protein 2 SMAD family member 2 JV18-1 protein protein human Homo sapiens Human MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS DIP protein Q15796 original sequence version: 174 DIP protein Q15796 original sequence version: 100 DIP protein Q15796-1 original sequence version: 141 95406DB5FC0AA4C9 ski_human Ski oncogene SKI Proto-oncogene c-Ski protein protein human Homo sapiens Human MEAAAGGRGCFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAAAVPAPVPAATEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINAVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYGNKYKRRVPRVSSEPPASIRPKTDDTSSQSPAPSEKDKPSSWLRTLAGSSNKSLGCVHPRQRLSAFRPWSPAVSASEKELSPHLPALIRDSFYSYKSFETAVAPNVALAPPAQQKVVSSPPCAAAVSRAPEPLATCTQPRKRKLTVDTPGAPETLAPVAAPEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLGSPGARALPSAVPDAAAPADAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLSAALQAKRSLHQELEFLRVAKKEKLREATEAKRNLRKEIERLRAENEKKMKEANESRLRLKRELEQARQARVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLLREREAREHLEKVVKELQEQLWPRARPEAAGSEGAAELEP DIP protein P12755 original sequence version: 126 DIP protein P12755 original sequence version: 130 9B78C4840A28C2DA pax3_human_gene Paired box protein Pax-3 PAX3 HUP2 gene gene human Homo sapiens Human smad4_human Mothers against decapentaplegic homolog 4 SMAD4 DPC4 MADH4 SMAD family member 4 Deletion target in pancreatic carcinoma 4 protein protein human Homo sapiens Human MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD DIP protein Q13485 original sequence version: 149 DIP protein Q13485 original sequence version: 177 7EE3C4647712DA90 ski-smad2-4 1 3 unspecified role unspecified role prey prey region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 4 unspecified role unspecified role bait bait physical association physical association 4C ski-smad2-5 1 6 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait association association 4D Nelfinavir (CHEBI:7496) -an HIV1-protease inhibitor;drug repositioning identified this drug as an anti-tumour agent. ski-pax3-1 2 5 unspecified role unspecified role unspecified role unspecified role promoter_smad_binding_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 4 unspecified role unspecified role unspecified role unspecified role association association 4E Nelfinavir (CHEBI:7496) -an HIV1-protease inhibitor;drug repositioning identified this drug as an anti-tumour agent. Selumetinib (CHEBI:90227)- a MEK inhibitor. CHEBI:7496 increases the binding of P12755 to ENSG00000135903 CHEBI:90227 decreases the binding of P12755 to ENSG00000135903 from Fig 5E CHEBI:90227 increases expession at RNA level of ENSG00000135903 CHEBI:90227 decreases expession at RNA level of P12755