IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers. Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers. Cancer cell 2016 imex curation Grandin M., Meier M., Delcros JG., Nikodemus D., Reuten R., Patel TR., Goldschneider D., Orriss G., Krahn N., Boussouar A., Abes R., Dean Y., Neves D., Bernet A., Depil S., Schneiders F., Poole K., Dante R., Koch M., Mehlen P., Stetefeld J. Cancer - Interactions investigated in the context of cancer jorg.stetefeld@ad.manitoba.ca,patrick.mehlen@lyon.unicancer.fr 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Grandin M., Meier M., Delcros JG., Nikodemus D., Reuten R., Patel TR., Goldschneider D., Orriss G., Krahn N., Boussouar A., Abes R., Dean Y., Neves D., Bernet A., Depil S., Schneiders F., Poole K., Dante R., Koch M., Mehlen P., Stetefeld J. Cancer - Interactions investigated in the context of cancer there is a crystal - but is a homomonomer. Their "Solid phase Binding Assay" protocol corresponds to our definition of ELISA - but their ELISA corresponds to our Solid phase Assay. jorg.stetefeld@ad.manitoba.ca,patrick.mehlen@lyon.unicancer.fr Accepted 2017-AUG-08 AT 13:52 BST AT 13:52 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers. Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers. Cancer cell 2016 imex curation Grandin M., Meier M., Delcros JG., Nikodemus D., Reuten R., Patel TR., Goldschneider D., Orriss G., Krahn N., Boussouar A., Abes R., Dean Y., Neves D., Bernet A., Depil S., Schneiders F., Poole K., Dante R., Koch M., Mehlen P., Stetefeld J. Cancer - Interactions investigated in the context of cancer jorg.stetefeld@ad.manitoba.ca,patrick.mehlen@lyon.unicancer.fr 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Grandin M., Meier M., Delcros JG., Nikodemus D., Reuten R., Patel TR., Goldschneider D., Orriss G., Krahn N., Boussouar A., Abes R., Dean Y., Neves D., Bernet A., Depil S., Schneiders F., Poole K., Dante R., Koch M., Mehlen P., Stetefeld J. Cancer - Interactions investigated in the context of cancer there is a crystal - but is a homomonomer jorg.stetefeld@ad.manitoba.ca,patrick.mehlen@lyon.unicancer.fr Accepted 2017-AUG-08 AT 13:52 BST AT 13:52 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers. Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers. Cancer cell 2016 imex curation Grandin M., Meier M., Delcros JG., Nikodemus D., Reuten R., Patel TR., Goldschneider D., Orriss G., Krahn N., Boussouar A., Abes R., Dean Y., Neves D., Bernet A., Depil S., Schneiders F., Poole K., Dante R., Koch M., Mehlen P., Stetefeld J. Cancer - Interactions investigated in the context of cancer jorg.stetefeld@ad.manitoba.ca,patrick.mehlen@lyon.unicancer.fr 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay tag alk phosphatase activity tag visualisation by alkaline phosphatase activity tag alk phosphatase activity Grandin M., Meier M., Delcros JG., Nikodemus D., Reuten R., Patel TR., Goldschneider D., Orriss G., Krahn N., Boussouar A., Abes R., Dean Y., Neves D., Bernet A., Depil S., Schneiders F., Poole K., Dante R., Koch M., Mehlen P., Stetefeld J. Cancer - Interactions investigated in the context of cancer there is a crystal - but is a homomonomer jorg.stetefeld@ad.manitoba.ca,patrick.mehlen@lyon.unicancer.fr Accepted 2017-AUG-08 AT 13:52 BST AT 13:52 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation unc5b_rat Netrin receptor UNC5B Unc5b Unc5h2 Protein unc-5 homolog 2 Protein unc-5 homolog B protein protein rat Rattus norvegicus (Rat) Rat MRARSGARGALLLALLLCWDPTPSLAGIDSGGQALPDSFPSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQKGHVTQESLDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQACQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSRPLEPSGDVALYAGLVVAVFVVLAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYDSSTIGSGAGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLTIPGTGVSLLVPNGAIPQGKFYDLYLRINKTESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFTGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPAALKEVLELERTLGGYLVEEPKTLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQKALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAATTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMTTDGDC DIP protein O08722 original sequence version: 100 6E9C2A262E560B9B net1_mouse Netrin-1 Ntn1 protein protein mouse Mus musculus Mouse MMRAVWEALAALAAVACLVGAVRGGPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA 135120DB6F0482F3 o95631-pro_0000017082 Netrin-1 Epididymis tissue protein Li 131P NTN1 NTN1L protein protein human Homo sapiens Human GPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA 25 604 C15249F3797EA8BA (arrsr)x3 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARRSRARRSRARRSR q8k1s3-1 Netrin receptor UNC5B Protein unc-5 homolog 2 Protein unc-5 homolog B Unc5b Unc5h2 protein protein mouse Mus musculus Mouse MRARSGVRSALLLALLLCWDPTPSLAGVDSAGQVLPDSYPSAPAEQLPYFLLEPQDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQNDHVTQESLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSHPLETSGDVALYAGLVVAVFVVVAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPNNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYNSSTIGSGSGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLSLPGTGVSLLVPNGAIPQGKFYDLYLHINKAESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFMGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQRALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAITTQLGPYAFKIPLSIRQKICSSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMATDGDC 80E896F0F0E06012 net1_human Netrin-1 NTN1 NTN1L Epididymis tissue protein Li 131P protein protein human Homo sapiens Human MMRAVWEALAALAAVACLVGAVRGGPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA DIP protein O95631 original sequence version: 69 DIP protein O95631 original sequence version: 86 9827C09D0D783B27 unc5b-ntn1-2 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O09118:p.[Arg351Ala;Asn353Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain N A Wed May 02 19:57:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg351ala,asn353ala' Tue Jun 30 18:21:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg351Ala;Asn353Ala]' O09118:p.Arg348_Arg349delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain RR AA Wed May 02 19:43:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg348-349ala_ala' Tue Jun 30 16:21:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg348_Arg349delinsAlaAla' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range IgG1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 2A-C/G-H Fig 2 g/H - netrin must be the ligand as its concentration is varying. ntn1-unc5b-2 1 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 2D unc5b-ntn1-3 1 7 competitor competitor competitor prey prey 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 3A unc5b-ntn1-4 1 6 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 4 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc direct interaction direct interaction 4B NET1-H-mAb antibody; a humanised netrin-1-specific IgG1 monoclonal antibody. I have added an IC50 for the antibody interaction - but not sure if I should have done as it is not an interactor (sequence not given) unc5b-ntn1-5 3 4 unspecified role unspecified role bait bait c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 9 unspecified role unspecified role prey prey region alk phosphatase tag alkaline phosphatase tag alk phosphatase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O95631:p.Asn353Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Wed May 02 18:53:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn353ala' Tue Jun 30 18:19:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn353Ala' O95631:p.[Arg348_Arg349delinsAlaAla;Arg351Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain RR AA certain certain sequence position certain certain certain sequence position certain R A Thu Dec 08 14:34:51 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg348ala,arg349ala,arg351ala' Wed May 02 15:01:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg348-349ala_ala,arg351ala' Tue Jun 30 18:08:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg348_Arg349delinsAlaAla;Arg351Ala]' O95631:p.Lys358Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K L Wed May 02 18:46:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys358leu' Tue Jun 30 17:00:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys358Leu' O95631:p.Ser360Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:16:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser360ala' Tue Jun 30 16:49:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser360Ala' O95631:p.Arg348Trp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R W Wed May 02 19:45:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg348trp' Tue Jun 30 17:15:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg348Trp' O95631:p.Tyr357Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 20:09:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr357phe' Tue Jun 30 18:18:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr357Phe' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 4E unc5b-(arrsr)x3-1 2 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp 2A