IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mammary gland human mammary gland Human mammary_gland mammary gland fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS Cancer cell 2015 Only protein-protein interactions imex curation imex curation TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS Cancer cell 2015 Only protein-protein interactions imex curation imex curation TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS human placental cDNA library Cancer cell 2015 Only protein-protein interactions imex curation imex curation TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hmle Homo sapiens hmle Human immortalized mammary epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS Cancer cell 2015 Only protein-protein interactions imex curation imex curation TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS Cancer cell 2015 Only protein-protein interactions imex curation imex curation TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS Cancer cell 2015 Only protein-protein interactions imex curation imex curation TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS Cancer cell 2015 Only protein-protein interactions imex curation imex curation TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. TGIF Governs a Feed-Forward Network that Empowers Wnt Signaling to Drive Mammary Tumorigenesis. Cancer cell 2015 imex curation Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hmle Homo sapiens hmle Human immortalized mammary epithelial cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer I could have added more causality statements if the origin of Wnt3a was given here. Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Accepted 2015-NOV-23 AT 16:58 GMT AT 16:58 GMT by PPORRAS Cancer cell 2015 Only protein-protein interactions imex curation imex curation axin2_human Axin-2 AXIN2 Axin-like protein Axis inhibition protein 2 Conductin protein protein human Homo sapiens Human MSSAMLVTCLPDPSSSFREDAPRPPVPGEEGETPPCQPGVGKGQVTKPMSVSSNTRRNEDGLGEPEGRASPDSPLTRWTKSLHSLLGDQDGAYLFRTFLEREKCVDTLDFWFACNGFRQMNLKDTKTLRVAKAIYKRYIENNSIVSKQLKPATKTYIRDGIKKQQIDSIMFDQAQTEIQSVMEENAYQMFLTSDIYLEYVRSGGENTAYMSNGGLGSLKVVCGYLPTLNEEEEWTCADFKCKLSPTVVGLSSKTLRATASVRSTETVDSGYRSFKRSDPVNPYHIGSGYVFAPATSANDSEISSDALTDDSMSMTDSSVDGIPPYRVGSKKQLQREMHRSVKANGQVSLPHFPRTHRLPKEMTPVEPATFAAELISRLEKLKLELESRHSLEERLQQIREDEEREGSELTLNSREGAPTQHPLSLLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHHHHSQYHSLLPPGGKLPPAAASPGACPLLGGKGFVTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVHCFCPGGSEYYCYSKCKSHSKAPETMPSEQFGGSRGSTLPKRNGKGTEPGLALPAREGGAPGGAGALQLPREEGDRSQDVWQWMLESERQSKPKPHSAQSTKKAYPLESARSSPGERASRHHLWGGNSGHPRTTPRAHLFTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPPKQRCCVASQQRDRNHSATVQTGATPFSNPSLAPEDHKEPKKLAGVHALQASELVVTYFFCGEEIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWEDETVLPMYEGRILGKVERID DIP protein Q9Y2T1 original sequence version: 142 DIP protein Q9Y2T1 original sequence version: 110 F7B62BED6AB4664D xpo1_mouse Exportin-1 Xpo1 Crm1 Chromosome region maintenance 1 protein homolog protein protein mouse Mus musculus Mouse MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKAKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFETLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHGQLLEKRLNLREALMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDIPPRRQLYLTVLSKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTEIIMTKKLQNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPAQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTPLNPGNPVNNQMFIQDYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEERETALRQAQEEKHKLQMSVPGILNPHEIPEEMCD DIP protein Q6P5F9 original sequence version: 66 DIP protein Q6P5F9 original sequence version: 90 DIP protein Q6P5F9 original sequence version: 70 B451A19780024562 tgif1_human Homeobox protein TGIF1 5'-TG-3'-interacting factor 1 TGIF1 TGIF protein protein human Homo sapiens Human MVLAQSRVSAGVGSPHCSGSGGGGSDSFPWPASHPGNPQCSFSTAFLASPRLSRGTLAYLPPAPWSSLATPSALLGSSCAPPPPPARCPQPRALSPELGTKAGPRRPHRWELPRSPSQGAQGPAPRRRLLETMKGIVAASGSETEDEDSMDIPLDLSSSAGSGKRRRRGNLPKESVQILRDWLYEHRYNAYPSEQEKALLSQQTHLSTLQVCNWFINARRRLLPDMLRKDGKDPNQFTISRRGAKISETSSVESVMGIKNFMPALEETPFHSCTAGPNPTLGRPLSPKPSSPGSVLARPSVICHTTVTALKDVPFSLCQSVGVGQNTDIQQIAAKNFTDTSLMYPEDTCKSGPSTNTQSGLFNTPPPTPPDLNQDFSGFQLLVDVALKRAAEMELQAKLTA 4D9C76AFB37A29F0 axin2_mouse Axin-2 Axin2 Axis inhibition protein 2 Conductin Axin-like protein protein protein mouse Mus musculus Mouse MSSAVLVTLLPDPSSSFREDAPRPPVPGEEGETPPCQPSVGKVQSTKPMPVSSNARRNEDGLGEPEGRASPDSPLTRWTKSLHSLLGDQDGAYLFRTFLEREKCVDTLDFWFACNGFRQMNLKDTKTLRVAKAIYKRYIENNSVVSKQLKPATKTYIRDGIKKQQIGSVMFDQAQTEIQAVMEENAYQVFLTSDIYLEYVRSGGENTAYMSNGGLGSLKVLCGYLPTLNEEEEWTCADLKCKLSPTVVGLSSKTLRATASVRSTETAENGFRSFKRSDPVNPYHVGSGYVFAPATSANDSELSSDALTDDSMSMTDSSVDGVPPYRMGSKKQLQREMHRSVKANGQVSLPHFPRTHRLPKEMTPVEPAAFAAELISRLEKLKLELESRHSLEERLQQIREDEEKEGSEQALSSRDGAPVQHPLALLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHQHHHHQQCHTLLPTGGKLPPVAACPLLGGKSFLTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVRCLCPGGTDYYCYSKCKSHPKAPEPLPGEQFCGSRGGTLPKRNAKGTEPGLALSARDGGMSSAAGAPQLPGEEGDRSQDVWQWMLESERQSKSKPHSAQSIRKSYPLESACAAPGERVSRHHLLGASGHSRSVARAHPFTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPQKQRCCVASQQRDRNHSAAGQAGASPFANPSLAPEDHKEPKKLASVHALQASELVVTYFFCGEEIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWDDETVLPMYEGRILGKVERID DIP protein O88566 original sequence version: 130 mint homomint Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B 53D7D33128879298 ctnb1_mouse Catenin beta-1 Beta-catenin Ctnnb1 Catnb protein protein mouse Mus musculus Mouse MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein Q02248 original sequence version: 128 DIP protein Q02248 original sequence version: 135 DIP protein Q02248 original sequence version: 147 DIP protein Q02248 original sequence version: 187 DIP protein Q02248 original sequence version: 140 D708F170A3FBED6E fbw1a_mouse F-box/WD repeat-containing protein 1A Btrc Fbw1 Fbxw1 Fwd1 Kiaa4123 Beta-TrCP protein E3RS-IkappaB Beta-transducin repeat-containing protein E3RSIkappaB F-box and WD repeats protein beta-TrCP HOS Ubiquitin ligase FWD1 pIkappaB-E3 receptor subunit protein protein mouse Mus musculus Mouse MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCINQETVCLTSTAMKTENCVAKAKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDENAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKSTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNAGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLMAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAHAEPPRSPSRTYTYISR 9E66D94D1EEEFF1B tf7l2_human Transcription factor 7-like 2 TCF7L2 TCF4 HMG box transcription factor 4 T-cell-specific transcription factor 4 protein protein human Homo sapiens Human MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTLHFQSGSTHYSAYKTIEHQIAVQYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWLVPQQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKRDKQPGETNEHSECFLNPCLSLPPITDLSAPKKCRARFGLDQQNNWCGPCRRKKKCVRYIQGEGSCLSPPSSDGSLLDSPPPSPNLLGSPPRDAKSQTEQTQPLSLSLKPDPLAHLSMMPPPPALLLAEATHKASALCPNGALDLPPAALQPAAPSSSIAQPSTSSLHSHSSLAGTQPQPLSLVTKSLE DIP protein Q9NQB0 original sequence version: 101 DIP protein Q9NQB0 original sequence version: 99 DIP protein Q9NQB0 original sequence version: 116 4DD2D3CC814AE16E axin1_mouse Axin-1 Axin1 Axin Fu Axis inhibition protein 1 Protein Fused protein protein mouse Mus musculus Mouse MNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSETETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD 2216D66E92387B81 cadh1_mouse Cadherin-1 Epithelial cadherin Uvomorulin ARC-1 Cdh1 protein protein mouse Mus musculus Mouse MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVPERHLERGHVLGRVRFEGCTGRPRTAFFSEDSRFKVATDGTITVKRHLKLHKLETSFLVRARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPELLMFPSVYPGLRRQKRDWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVNARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQQYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEITSYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVALIIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTTLDVHVCDCEGTVNNCMKAGIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVKEPLLPPDDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPQYRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDQDQDYDYLNEWGNRFKKLADMYGGGEDD DIP protein P09803 original sequence version: 92 DIP protein P09803 original sequence version: 116 DIP protein P09803 original sequence version: 124 DIP protein P09803 original sequence version: 128 DIP protein P09803 original sequence version: 141 DIP protein P09803 original sequence version: 174 7A6444148D3D5983 fbw1a_human F-box/WD repeat-containing protein 1A BTRC BTRCP FBW1A FBXW1A F-box and WD repeats protein beta-TrCP E3RSIkappaB pIkappaBalpha-E3 receptor subunit Epididymis tissue protein Li 2a protein protein human Homo sapiens Human MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR DIP protein Q9Y297 original sequence version: 142 DIP protein Q9Y297 original sequence version: 153 DIP protein Q9Y297 original sequence version: 144 DIP protein Q9Y297 original sequence version: 145 DIP protein Q9Y297 original sequence version: 106 DIP protein Q9Y297 original sequence version: 82 DIP protein Q9Y297 original sequence version: 111 DIP protein Q9Y297 original sequence version: 125 4C67F3B7E400FD37 tgif1_human_gene Homeobox protein TGIF1 TGIF1 TGIF gene gene human Homo sapiens Human axin1_human Axin-1 Axis inhibition protein 1 AXIN1 AXIN protein protein human Homo sapiens Human MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD DIP protein O15169 original sequence version: 118 DIP protein O15169 original sequence version: 125 DIP protein O15169-1 original sequence version: 140 DIP protein O15169 original sequence version: 132 10779173F5092F3F ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E tgif1-axin2-2 3 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait N/A sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure tgif1-axin2-1 2 12 unspecified role unspecified role prey prey N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1A, Supp 1A Wnt3a tgif1-axin2-3 2 16 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1D Wnt3a tgif1-axin1-1 7 9 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 1E Wnt3a tgif1-axin1-2 4 9 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 1 E/F Wnt3a tgif1-axin2-4 6 12 unspecified role unspecified role neutral component neutral component N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component N/A gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 2A tgif1-axin1-3 5 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey association association 2D TGIF deficiency increases the interaction of Axin1 and 2 with beta-catenin. ctnnb1-axin1-8 5 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey association association 2E TGIF deficiency increases the interaction of Axin1 and 2 with beta-catenin. ctnnb1-axin1-9 5 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait putative phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm physical association physical association 3A TGIF deficiency increases the interaction of Axin1 and 2 with beta-catenin. btrc-1 7 18 unspecified role unspecified role bait bait 21 unspecified role unspecified role prey prey physical association physical association 3D Induction of TGIF expression resulted in decreased interaction of beta-catenin with beta-TRCP. ctnnb1-1 8 21 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey N/A sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5B Wnt3a P35222 increases expression at protein level of ENSG00000177426 P35222 increases expression at RNA level of ENSG00000177426 tcf7l2-tgif1-1 8 15 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey N/A sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5Bi Wnt3a Q9NQB0 increases expression at RNA level of ENSG00000177426 Q9NQB0 increases expression at protein level of ENSG00000177426 tgif1-axin2-5 2 12 unspecified role unspecified role prey prey N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range N/A sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N/A required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supp 1B/C Wnt3a axin1-tgif1-1 2 16 unspecified role unspecified role prey prey N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 1D Wnt3a tgif1-axin1-4 7 20 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Supp 1E Wnt3a axin1-axin2-1 7 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Supp 1Ei Wnt3a axin2-tgif1-1 4 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Supp 1Ei Wnt3a axin1-tgif1-2 4 20 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Supp 1E Wnt3a ctnnb1-tgif1-1 1 21 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization S3C the co-expression of TGIF and beta-catenin is mainly confined to the whole epithelial duct structure.