IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mcf12a human-mcf12a-Human Immortalised non-tumorigenic human mammary epithelial cells Human mcf12a MCF12A Human Immortalised non-tumorigenic human mammary epithelial cells http://www.lgcstandards-atcc.org/products/all/CRL-10782.aspx?geo_country=gb#generalinformation anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Accepted 2015-JUN-01 by ORCHARD Cancer cell 2015 imex curation 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mcf12a human-mcf12a-Human Immortalised non-tumorigenic human mammary epithelial cells Human mcf12a MCF12A Human Immortalised non-tumorigenic human mammary epithelial cells http://www.lgcstandards-atcc.org/products/all/CRL-10782.aspx?geo_country=gb#generalinformation confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Accepted 2015-JUN-01 by ORCHARD Cancer cell 2015 imex curation 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Accepted 2015-JUN-01 by ORCHARD Cancer cell 2015 imex curation 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Accepted 2015-JUN-01 by ORCHARD Cancer cell 2015 imex curation 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Accepted 2015-JUN-17 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation I have added an RNA and protein causality statement.; done 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Accepted 2015-JUN-01 by ORCHARD Cancer cell 2015 imex curation 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Accepted 2015-JUN-01 by ORCHARD Cancer cell 2015 imex curation 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Accepted 2015-JUN-17 by ORCHARD Cancer cell 2015 imex curation 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. 14-3-3ζ turns TGF-β's function from tumor suppressor to metastasis promoter in breast cancer by contextual changes of Smad partners from p53 to Gli2. Cancer cell 2015 imex curation Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Only protein-protein interactions imex curation human-mcf12a human-mcf12a-Human Immortalised non-tumorigenic human mammary epithelial cells Human mcf12a MCF12A Human Immortalised non-tumorigenic human mammary epithelial cells http://www.lgcstandards-atcc.org/products/all/CRL-10782.aspx?geo_country=gb#generalinformation anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Accepted 2015-JUN-01 by ORCHARD Cancer cell 2015 imex curation 1433s_human_gene 14-3-3 protein sigma SFN HME1 gene gene human Homo sapiens Human gli2_human Zinc finger protein GLI2 GLI2 THP GLI family zinc finger protein 2 Tax helper protein protein protein human Homo sapiens Human METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT 86556112E13DE106 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 yap1_human Transcriptional coactivator YAP1 YAP1 YAP65 Protein yorkie homolog Yes-associated protein YAP65 homolog protein protein human Homo sapiens Human MDPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL DIP protein P46937 original sequence version: 143 DIP protein P46937 original sequence version: 98 DIP protein P46937-1 original sequence version: 110 DIP protein P46937 original sequence version: 149 DIP protein P46937 original sequence version: 153 DIP protein P46937 original sequence version: 157 DIP protein P46937 original sequence version: 160 DIP protein P46937 original sequence version: 122 DIP protein P46937 original sequence version: 124 6145F7049ED338AE smad3_human Mothers against decapentaplegic homolog 3 SMAD3 MADH3 SMAD family member 3 JV15-2 protein protein human Homo sapiens Human MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS DIP protein P84022 original sequence version: 57 DIP protein P84022 original sequence version: 112 DIP protein P84022-1 original sequence version: 146 DIP protein P84022-1 original sequence version: 111 46DF5E8B371321AC smad2_human Mothers against decapentaplegic homolog 2 SMAD2 MADH2 MADR2 Mad-related protein 2 SMAD family member 2 JV18-1 protein protein human Homo sapiens Human MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS DIP protein Q15796 original sequence version: 174 DIP protein Q15796 original sequence version: 100 DIP protein Q15796-1 original sequence version: 141 95406DB5FC0AA4C9 fbw1a_human F-box/WD repeat-containing protein 1A BTRC BTRCP FBW1A FBXW1A F-box and WD repeats protein beta-TrCP E3RSIkappaB pIkappaBalpha-E3 receptor subunit Epididymis tissue protein Li 2a protein protein human Homo sapiens Human MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR DIP protein Q9Y297 original sequence version: 142 DIP protein Q9Y297 original sequence version: 153 DIP protein Q9Y297 original sequence version: 144 DIP protein Q9Y297 original sequence version: 145 DIP protein Q9Y297 original sequence version: 106 DIP protein Q9Y297 original sequence version: 82 DIP protein Q9Y297 original sequence version: 111 DIP protein Q9Y297 original sequence version: 125 4C67F3B7E400FD37 smad4_human Mothers against decapentaplegic homolog 4 SMAD4 DPC4 MADH4 SMAD family member 4 Deletion target in pancreatic carcinoma 4 protein protein human Homo sapiens Human MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD DIP protein Q13485 original sequence version: 149 DIP protein Q13485 original sequence version: 177 7EE3C4647712DA90 1433z_human 14-3-3 protein zeta/delta YWHAZ Protein kinase C inhibitor protein 1 protein protein human Homo sapiens Human MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN DIP protein P63104 original sequence version: 144 D464DF2286BBFE60 smad2-tp53-1 4 15 unspecified role unspecified role bait bait N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 12 unspecified role unspecified role prey prey physical association physical association 1G TGF-beta Transfected 14-3-3-zeta yap1-sfn-1 8 10 unspecified role unspecified role prey prey N/A sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm association association 2E 14-3-3-zeta overexpression P46937 increases expression at RNA level of ENSG00000175793 P46937 increases expression at protein level of ENSG00000175793 ywhaz-yap1-1 7 13 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 18 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2F ywhaz-yap1-2 1 13 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 18 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2G ywhaz-yap1-3 6 13 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 2H 245twt-1 3 14 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 5I you can see (just about) two bands for the Smads, in the correct M.Wt - so have entered them. TGF-beta TGF-beta treatment did not induce Gli2 binding to Smads in 14-3-3-zeta shRNA transfected cells. 14-3-3-zeta shRNA. TGF-beta treatment is shown here to increase the phosphorylation of both Smad 2 and 3 - only no antibody is listed for the dual target antibody in the supplementary "Antibody List", thus it is not possible to determine if one or both Smads were phosphorylated. smad2-tp53-2 4 15 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 12 unspecified role unspecified role bait bait physical association physical association Supp 1J TGF-beta Transfected 14-3-3-zeta smad2-tp53-3 9 15 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 12 unspecified role unspecified role bait bait physical association physical association Supp 1J TGF-beta Transfected 14-3-3-zeta ywhaz-yap1-4 4 13 unspecified role unspecified role bait bait N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 18 unspecified role unspecified role prey prey physical association physical association Supp 2F yap1-ywhaz-1 2 13 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Supp 2G w57w5-1 3 16 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Supp Fig 5R Cells were pre-treated with TGF-beta and MG132 - a proteasome inhibitor. Gli2 binding to beta-TrCP was only evident in cells transfected with shRNA to 14-3-3-zeta, but not in cells transfected with control shRNA. gli2-smad2-1 3 14 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Supp 5T gli2-1 3 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey physical association physical association 5F 2454r2rwq-1 5 16 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5F/G Gli2 binding to beta-Trcp was weaker in control shRNA cells than in 14-3-3-zeta knockout cells. gli2-btrc-1 5 16 competitor competitor competitor prey prey N/A gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5G gli2-smad2-2 5 17 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 15 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 11 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp 5S TGF-beta shCtrl cells were used gli2-btrc-2 5 16 competitor competitor competitor prey prey N/A gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5Gi