IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Drugging MYCN through an allosteric transition in Aurora kinase A. Drugging MYCN through an allosteric transition in Aurora kinase A. Cancer Cell (1535-6108) 2014 imex curation Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com,gustafsonc@peds.ucsf.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-imr_32 Homo sapiens:Neuroblastoma cell line imr_32 Human IMR-32 Neuroblastoma Human neuroblastoma cell line established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old Caucasian boy in 1967. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Accepted 2014-OCT-30 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Drugging MYCN through an allosteric transition in Aurora kinase A. Drugging MYCN through an allosteric transition in Aurora kinase A. Cancer Cell (1535-6108) 2014 imex curation Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com,gustafsonc@peds.ucsf.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer In Supplementary figure 1B and C legend it is stated that:- "Enzyme was either full-length or kinase domain-only Aurora A, and substrate was either full-length purified Histone H3 or target oligopeptide." The target oligopeptide has been entered here as the exact identity of the H3 molecule used is unknown and the origin of full length Aurora A is not stated. waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Accepted 2014-OCT-30 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Drugging MYCN through an allosteric transition in Aurora kinase A. Drugging MYCN through an allosteric transition in Aurora kinase A. Cancer Cell (1535-6108) 2014 imex curation Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com,gustafsonc@peds.ucsf.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Accepted 2014-OCT-30 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Drugging MYCN through an allosteric transition in Aurora kinase A. Drugging MYCN through an allosteric transition in Aurora kinase A. Cancer Cell (1535-6108) 2014 imex curation Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com,gustafsonc@peds.ucsf.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer PDBe entry 4J8N, describes what might be a homotetramer - but the 4-ry structure states it might be a monomer - so not entered. waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Accepted 2014-OCT-30 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK DIP protein P62753 original sequence version: 72 DIP protein P62753 original sequence version: 68 DIP protein P62753 original sequence version: 123 A61E435884E636AE aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS DIP protein O14965 original sequence version: 181 DIP protein O14965 original sequence version: 146 DIP protein O14965 original sequence version: 124 DIP protein O14965 original sequence version: 160 DIP protein O14965 original sequence version: 138 DIP protein O14965 original sequence version: 98 125F3594834CD157 max_human Protein max MAX BHLHD4 Class D basic helix-loop-helix protein 4 Myc-associated factor X protein protein human Homo sapiens Human MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS DIP protein P61244 original sequence version: 132 DIP protein P61244 original sequence version: 128 DIP protein P61244 original sequence version: 150 DIP protein P61244 original sequence version: 120 EB10F3137727A56F cd532 CD532 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC DIP protein P04198 original sequence version: 157 560E885602E30DAD mycn-aurka-2 1 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 8 A/B CD532 - an ATP-mimetic molecule, and Conformation-Disrupting Aurora A inhibitor, which causes a dose-dependent and complete dissociation of the MYCN-Aurora-A complex. CD532 MLN8237 -a small molecule specific Aurora A inhibitor, and a less effective disruptor of the this interaction than CD532. aurka-cd532 4 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component kinase_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14965:p.Thr287Asp mutation mutation certain certain sequence position certain certain certain sequence position certain T D Wed May 02 19:49:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr287asp' Tue Jun 30 18:15:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr287Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Table 1 (fig 3) Working: 17.82%, Free: 21.8% 1.853 A aurka-cd532-2 2 5 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 enzyme enzyme neutral component neutral component kinase_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14965:p.Thr287Asp mutation mutation certain certain sequence position certain certain certain sequence position certain T D Wed May 02 20:22:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr287asp' Tue Jun 30 17:22:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr287Asp' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Supplementary Fig 1B/C P62753 opser CHEBI:83432 decreases activity of O14965 mycn-aurka-4 3 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Supp 6A/B CD532 causes a dissociation of the MYCN-Aurora-A complex; the effect of CD532 on the MYCN-Aurora-A interaction was specific in that it did not affect MYCN-MAX binding.