IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. Cell research 2014 imex curation Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Accepted 2017-MAR-01 AT 13:27 GMT AT 13:27 GMT by ORCHARD Cell research 2014 imex curation EBI-12739702:- I have made this into 3 interactions:- (EBI-13918613) is Full length protein as in Fig 2A and last 2 lanes in fig 2D. EBI-13637126:- I have split into 2 - although not sure if this is what you wanted.; altered The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. Cell research 2014 imex curation Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Only protein-protein interactions human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Accepted 2017-MAR-01 AT 13:27 GMT AT 13:27 GMT by ORCHARD Cell research 2014 imex curation EBI-12739702:- I have made this into 3 interactions:- (EBI-13918613) is Full length protein as in Fig 2A and last 2 lanes in fig 2D. EBI-13637126:- I have split into 2 - although not sure if this is what you wanted.; altered The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. Cell research 2014 imex curation Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? Cells were treated with EGF The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Accepted 2017-MAR-01 AT 13:27 GMT AT 13:27 GMT by ORCHARD Cell research 2014 imex curation EBI-12739702:- I have made this into 3 interactions:- (EBI-13918613) is Full length protein as in Fig 2A and last 2 lanes in fig 2D. EBI-13637126:- I have split into 2 - although not sure if this is what you wanted.; altered The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. Cell research 2014 imex curation Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Accepted 2017-MAR-01 AT 13:27 GMT AT 13:27 GMT by ORCHARD Cell research 2014 imex curation EBI-12739702:- I have made this into 3 interactions:- (EBI-13918613) is Full length protein as in Fig 2A and last 2 lanes in fig 2D. EBI-13637126:- I have split into 2 - although not sure if this is what you wanted.; altered The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. Cell research 2014 imex curation Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Wang HM., Xu YF., Ning SL., Yang DX., Li Y., Du YJ., Yang F., Zhang Y., Liang N., Yao W., Zhang LL., Gu LC., Gao CJ., Pang Q., Chen YX., Xiao KH., Ma R., Yu X., Sun JP. Cancer - Interactions investigated in the context of cancer DSP= no 66? Two different method were used to assay the PTPN18-catalysed hydrolysis of HER2 pY -containing peptides. The first, showed the increase in tyrosine fluorescence after dephosphorylation, the second an assay for free inorganic phosphate. The dephosphorylation of pNPP was monitored by measuring the increase in absorbance at 405 nm - at which wavelength the product, p-nitrophenol, absorbs. The authors have stated that HA-CBL and HA-TRCP -as used in figs 8/9, are of Human origin.They have also confirmed that the N term amino acids in the HER2 peptides areacetylated. The amino acid range for the HER2 peptides was taken from Table S1 for Phosphatase assays, and the main text of the publication, under Figure 3 . yuxiao@sdu.edu.cn,sunjinpeng@sdu.edu.cn Accepted 2017-MAR-01 AT 13:27 GMT AT 13:27 GMT by ORCHARD Cell research 2014 imex curation EBI-12739702:- I have made this into 3 interactions:- (EBI-13918613) is Full length protein as in Fig 2A and last 2 lanes in fig 2D. EBI-13637126:- I have split into 2 - although not sure if this is what you wanted.; altered q99952-1 Tyrosine-protein phosphatase non-receptor type 18 Brain-derived phosphatase PTPN18 BDP1 protein protein human Homo sapiens Human MSRSLDSARSFLERLEARGGREGAVLAGEFSDIQACSAAWKADGVCSTVAGSRPENVRKNRYKDVLPYDQTRVILSLLQEEGHSDYINGNFIRGVDGSLAYIATQGPLPHTLLDFWRLVWEFGVKVILMACREIENGRKRCERYWAQEQEPLQTGLFCITLIKEKWLNEDIMLRTLKVTFQKESRSVYQLQYMSWPDRGVPSSPDHMLAMVEEARRLQGSGPEPLCVHCSAGCGRTGVLCTVDYVRQLLLTQMIPPDFSLFDVVLKMRKQRPAAVQTEEQYRFLYHTVAQMFCSTLQNASPHYQNIKENCAPLYDDALFLRTPQALLAIPRPPGGVLRSISVPGSPGHAMADTYAVVQKRGAPAGAGSGTQTGTGTGTGARSAEEAPLYSKVTPRAQRPGAHAEDARGTLPGRVPADQSPAGSGAYEDVAGGAQTGGLGFNLRIGRPKGPRDPPAEWTRV 67ED24A0504D1883 cbl_human E3 ubiquitin-protein ligase CBL Signal transduction protein CBL Proto-oncogene c-Cbl Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 CBL CBL2 RNF55 RING-type E3 ubiquitin transferase CBL protein protein human Homo sapiens Human MAGNVKKSSGAGGGSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT DIP protein P22681 original sequence version: 173 DIP protein P22681 original sequence version: 128 DIP protein P22681 original sequence version: 145 DIP protein P22681 original sequence version: 150 DIP protein P22681 original sequence version: 191 DIP protein P22681 original sequence version: 91 DIP protein P22681 original sequence version: 139 1AA6BF67377322CA ptn18_human Tyrosine-protein phosphatase non-receptor type 18 Brain-derived phosphatase PTPN18 BDP1 protein protein human Homo sapiens Human MSRSLDSARSFLERLEARGGREGAVLAGEFSDIQACSAAWKADGVCSTVAGSRPENVRKNRYKDVLPYDQTRVILSLLQEEGHSDYINGNFIRGVDGSLAYIATQGPLPHTLLDFWRLVWEFGVKVILMACREIENGRKRCERYWAQEQEPLQTGLFCITLIKEKWLNEDIMLRTLKVTFQKESRSVYQLQYMSWPDRGVPSSPDHMLAMVEEARRLQGSGPEPLCVHCSAGCGRTGVLCTVDYVRQLLLTQMIPPDFSLFDVVLKMRKQRPAAVQTEEQYRFLYHTVAQMFCSTLQNASPHYQNIKENCAPLYDDALFLRTPQALLAIPRPPGGVLRSISVPGSPGHAMADTYAVVQKRGAPAGAGSGTQTGTGTGTGARSAEEAPLYSKVTPRAQRPGAHAEDARGTLPGRVPADQSPAGSGAYEDVAGGAQTGGLGFNLRIGRPKGPRDPPAEWTRV 67ED24A0504D1883 erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV DIP protein P04626 original sequence version: 198 DIP protein P04626 original sequence version: 122 DIP protein P04626 original sequence version: 206 DIP protein P04626 original sequence version: 195 DIP protein P04626 original sequence version: 150 DIP protein P04626 original sequence version: 179 DIP protein P04626 original sequence version: 146 DIP protein P04626 original sequence version: 147 39E9DFDA04DCF962 4-nitrophenyl phosphate(2−) 4-nitrophenyl phosphate(2−) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ptn1_human Tyrosine-protein phosphatase non-receptor type 1 PTPN1 PTP1B Protein-tyrosine phosphatase 1B protein protein human Homo sapiens Human MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT DIP protein P18031 original sequence version: 141 DIP protein P18031 original sequence version: 191 802377DCD33F41FD fbw1a_human F-box/WD repeat-containing protein 1A BTRC BTRCP FBW1A FBXW1A F-box and WD repeats protein beta-TrCP E3RSIkappaB pIkappaBalpha-E3 receptor subunit Epididymis tissue protein Li 2a protein protein human Homo sapiens Human MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR DIP protein Q9Y297 original sequence version: 142 DIP protein Q9Y297 original sequence version: 153 DIP protein Q9Y297 original sequence version: 144 DIP protein Q9Y297 original sequence version: 145 DIP protein Q9Y297 original sequence version: 106 DIP protein Q9Y297 original sequence version: 82 DIP protein Q9Y297 original sequence version: 111 DIP protein Q9Y297 original sequence version: 125 4C67F3B7E400FD37 ptpn18-erbb2-1 2 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range pest_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2 D EGF ptpn18-erbb2-2 2 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait Q99952-1:p.Cys229Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:49:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys229ser' Tue Jun 30 19:23:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys229Ser' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2D ptpn18-erbb2-3 3 9 unspecified role unspecified role prey prey human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 6 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99952-1:p.Cys229Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:42:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys229ser' Tue Jun 30 19:57:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys229Ser' ecoli Escherichia coli (strain K12) physical association physical association 2E ptpn18-erbb2-4 3 9 unspecified role unspecified role prey prey human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 6 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pest_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 2 E godknows-1 1 9 unspecified role unspecified role neutral component neutral component glu1244 acglu N-acetyl-L-glutamic acid (2S)-2-(acetamido)pentanedioic acid 2-(acetylamino)pentanedioic acid 2-(acetylazanyl)pentanedioic acid MOD_RES N-acetylglutamate N-acetyl-L-glutamic acid N-acetylated L-glutamic acid acetylglutamic acid AcGlu certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tyr1248 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 8 unspecified role unspecified role neutral component neutral component possible_catalytic_domain sufficient to bind sufficient binding region sufficient to bind range range range range Q99952:p.Cys229Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:33:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys229ser' Tue Jun 30 16:53:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys229Ser' ecoli Escherichia coli (strain K12) 41 38 direct interaction direct interaction Supp Table 2 2.0A R work =0.19 R free= 0.23 erbb2-ptpn18-1 1 9 unspecified role unspecified role neutral component neutral component glu1192 acglu N-acetyl-L-glutamic acid (2S)-2-(acetamido)pentanedioic acid 2-(acetylamino)pentanedioic acid 2-(acetylazanyl)pentanedioic acid MOD_RES N-acetylglutamate N-acetyl-L-glutamic acid N-acetylated L-glutamic acid acetylglutamic acid AcGlu certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tyr1196 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 8 unspecified role unspecified role neutral component neutral component possible_catalytic_domain sufficient to bind sufficient binding region sufficient to bind range range range range Q99952:p.Cys229Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:34:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys229ser' Tue Jun 30 19:25:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys229Ser' ecoli Escherichia coli (strain K12) 46 49 direct interaction direct interaction Supp Table 2-2 2.1A R work =0.25 R free= 0.29 erbb2-ptpn18-2 1 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tyr1196 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm pro1108 nacpro N-acetyl-L-proline (2S)-1-acetyl-2-pyrrolidinecarboxylic acid 1-acetylproline MOD_RES N-acetylproline N-acetyl-L-proline N-acetylated L-proline N-acetylproline acetylproline NAcPro certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 8 unspecified role unspecified role neutral component neutral component possible_catalytic_domain sufficient to bind sufficient binding region sufficient to bind range range range range Q99952:p.Cys229Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:09:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys229ser' Tue Jun 30 18:04:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys229Ser' ecoli Escherichia coli (strain K12) 53 57 direct interaction direct interaction Supp Table 2-3 2.5A R work =0.26 R free= 0.29 The amino acid range for the HER2-pY1112 peptide was taken from Table S1 for Phosphatase assays. erbb2-ptpn18-3 5 9 enzyme target enzyme target substrate substrate neutral component neutral component pro1108 nacpro N-acetyl-L-proline (2S)-1-acetyl-2-pyrrolidinecarboxylic acid 1-acetylproline MOD_RES N-acetylproline N-acetyl-L-proline N-acetylated L-proline N-acetylproline acetylproline NAcPro certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm tyr1112 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm P04626:p.Pro1116del mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain P . Wed May 02 15:20:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro1116del' Tue Jun 30 17:51:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro1116del' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 enzyme enzyme neutral component neutral component Q99952:p.Gln34Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:18:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln34ala' Tue Jun 30 16:23:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln34Ala' Q99952:p.Asp64Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:15:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp64ala' Tue Jun 30 19:51:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp64Ala' Q99952:p.Val65Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V A Wed May 02 14:57:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ala' Tue Jun 30 18:25:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ala' Q99952:p.Val65Ile mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V I Wed May 02 14:34:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ile' Tue Jun 30 17:08:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ile' Q99952:p.Pro109Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P A Wed May 02 16:30:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro109ala' Tue Jun 30 19:09:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro109Ala' Q99952:p.Arg138Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:37:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138ala' Tue Jun 30 19:50:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Ala' Q99952:p.Arg138Leu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L Wed May 02 19:23:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138leu' Tue Jun 30 17:53:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Leu' Q99952:p.Arg140Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:32:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg140ala' Tue Jun 30 20:23:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg140Ala' Q99952:p.Arg198Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:24:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198ala' Tue Jun 30 17:46:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Ala' Q99952:p.Arg198Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R E Wed May 02 19:13:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198glu' Tue Jun 30 18:01:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Glu' Q99952:p.Arg268Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:44:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg268ala' Tue Jun 30 16:42:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg268Ala' Q99952:p.Pro272Met mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P M Wed May 02 16:56:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro272met' Tue Jun 30 15:56:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro272Met' ecoli Escherichia coli (strain K12) direct interaction direct interaction 2F, Supp table 1, Fig 6B erbb2-ptpn18-6 5 9 enzyme target enzyme target substrate substrate neutral component neutral component glu1192 acglu N-acetyl-L-glutamic acid (2S)-2-(acetamido)pentanedioic acid 2-(acetylamino)pentanedioic acid 2-(acetylazanyl)pentanedioic acid MOD_RES N-acetylglutamate N-acetyl-L-glutamic acid N-acetylated L-glutamic acid acetylglutamic acid AcGlu certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm tyr1196 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 enzyme enzyme neutral component neutral component Q99952:p.Gln34Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 15:58:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln34ala' Tue Jun 30 15:53:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln34Ala' Q99952:p.Asp64Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:22:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp64ala' Tue Jun 30 16:05:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp64Ala' Q99952:p.Val65Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V A Wed May 02 15:11:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ala' Tue Jun 30 18:43:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ala' Q99952:p.Val65Ile mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V I Wed May 02 18:59:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ile' Tue Jun 30 17:28:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ile' Q99952:p.Pro109Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P A Wed May 02 20:21:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro109ala' Tue Jun 30 19:01:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro109Ala' Q99952:p.Arg138Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:53:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138ala' Tue Jun 30 19:05:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Ala' Q99952:p.Arg138Leu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L Wed May 02 19:49:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138leu' Tue Jun 30 16:01:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Leu' Q99952:p.Arg140Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:28:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg140ala' Tue Jun 30 19:51:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg140Ala' Q99952:p.Arg198Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:23:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198ala' Tue Jun 30 16:30:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Ala' Q99952:p.Arg198Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R E Wed May 02 17:45:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198glu' Tue Jun 30 19:22:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Glu' Q99952:p.Arg268Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:31:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg268ala' Tue Jun 30 19:41:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg268Ala' Q99952:p.Pro272Met mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P M Wed May 02 16:12:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro272met' Tue Jun 30 17:12:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro272Met' ecoli Escherichia coli (strain K12) direct interaction direct interaction 2F, Supp table 1, Fig 6B-2 erbb2-ptpn18-7 5 9 enzyme target enzyme target substrate substrate neutral component neutral component glu1244 acglu N-acetyl-L-glutamic acid (2S)-2-(acetamido)pentanedioic acid 2-(acetylamino)pentanedioic acid 2-(acetylazanyl)pentanedioic acid MOD_RES N-acetylglutamate N-acetyl-L-glutamic acid N-acetylated L-glutamic acid acetylglutamic acid AcGlu certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm tyr1248 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 enzyme enzyme neutral component neutral component Q99952:p.Gln34Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 17:41:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln34ala' Tue Jun 30 20:24:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln34Ala' Q99952:p.Asp64Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:21:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp64ala' Tue Jun 30 18:27:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp64Ala' Q99952:p.Val65Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V A Wed May 02 20:30:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ala' Tue Jun 30 18:50:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ala' Q99952:p.Val65Ile mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V I Wed May 02 19:10:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ile' Tue Jun 30 18:22:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ile' Q99952:p.Pro109Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P A Wed May 02 16:32:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro109ala' Tue Jun 30 16:49:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro109Ala' Q99952:p.Arg138Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:49:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138ala' Tue Jun 30 19:57:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Ala' Q99952:p.Arg138Leu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L Wed May 02 16:18:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138leu' Tue Jun 30 17:47:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Leu' Q99952:p.Arg140Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:18:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg140ala' Tue Jun 30 17:00:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg140Ala' Q99952:p.Arg198Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:38:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198ala' Tue Jun 30 20:17:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Ala' Q99952:p.Arg198Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R E Wed May 02 16:25:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198glu' Tue Jun 30 17:48:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Glu' Q99952:p.Arg268Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:05:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg268ala' Tue Jun 30 18:47:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg268Ala' Q99952:p.Pro272Met mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P M Wed May 02 17:21:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro272met' Tue Jun 30 19:56:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro272Met' ecoli Escherichia coli (strain K12) direct interaction direct interaction 2F, Supp table 1, Fig 6B-3 4_nitrophenyl phosphate(2)-ptpn18-1 5 10 enzyme target enzyme target substrate substrate neutral component neutral component 8 enzyme enzyme neutral component neutral component Q99952:p.Gln34Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:12:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln34ala' Tue Jun 30 20:22:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln34Ala' Q99952:p.Asp64Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:08:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp64ala' Tue Jun 30 16:57:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp64Ala' Q99952:p.Val65Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V A Wed May 02 16:38:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ala' Tue Jun 30 17:25:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ala' Q99952:p.Val65Ile mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V I Wed May 02 17:58:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65ile' Tue Jun 30 18:09:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val65Ile' Q99952:p.Pro109Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P A Wed May 02 15:38:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro109ala' Tue Jun 30 19:51:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro109Ala' Q99952:p.Arg138Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:26:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138ala' Tue Jun 30 19:19:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Ala' Q99952:p.Arg138Leu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L Wed May 02 18:14:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg138leu' Tue Jun 30 19:00:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg138Leu' Q99952:p.Arg140Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:02:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg140ala' Tue Jun 30 20:00:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg140Ala' Q99952:p.Arg198Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:37:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198ala' Tue Jun 30 18:38:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Ala' Q99952:p.Arg198Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R E Wed May 02 18:54:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg198glu' Tue Jun 30 17:09:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg198Glu' Q99952:p.Arg268Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:09:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg268ala' Tue Jun 30 18:50:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg268Ala' Q99952:p.Pro272Met mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P M Wed May 02 16:03:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro272met' Tue Jun 30 17:32:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro272Met' ecoli Escherichia coli (strain K12) direct interaction direct interaction 6B, Supp Fig 2 q46a5eta-1 4 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 10B ptpn18-btrc-1 4 12 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 10Bi 4_nitrophenyl phosphate(2)-ptpn18-2 5 10 enzyme target enzyme target substrate substrate neutral component neutral component 11 enzyme enzyme neutral component neutral component catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Fig 2 ptpn18-btrc-2 2 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait Q99952-1:p.Ser419Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:56:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser419ala' Tue Jun 30 18:33:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser419Ala' Q99952-1:p.Ser423Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:44:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser423ala' Tue Jun 30 17:39:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser423Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 8 I cbl-erbb2-1 2 9 unspecified role unspecified role prey prey tyr-1112 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 9 D-F PTPN18 and R140A (PTPN18) mutant PTPN18-D197A mutant ptpn18-erbb2-5 2 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait Q99952-1:p.Asp197Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:49:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp197ala' Tue Jun 30 16:10:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp197Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99952-1:p.Cys229Ser mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:55:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys229ser' Tue Jun 30 17:14:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys229Ser' physical association physical association 2 A+D EGF ptpn18-btrc-3 2 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range pest_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 8 H