IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Genomic Profiling of Hepatocellular Adenomas Reveals Recurrent FRK-Activating Mutations and the Mechanisms of Malignant Transformation. Genomic Profiling of Hepatocellular Adenomas Reveals Recurrent FRK-Activating Mutations and the Mechanisms of Malignant Transformation. Cancer Cell (1535-6108) 2014 imex curation Pilati C., Letouzé E., Nault J.C., Imbeaud S., Boulai A., Calderaro J., Poussin K., Franconi A., Couchy G., Morcrette G., Mallet M., Taouji S., Balabaud C., Terris B., Canal F., Paradis V., Scoazec J.Y., de Muret A., Guettier C., Bioulac-Sage P., Chevet E., Calvo F., Zucman-Rossi J. Cancer - Interactions investigated in the context of cancer Should the artificial peptides be curated at all? the Omnia 5 peptide "EAEAIYA" gives no results on Blasting. I have done them anyway. jessica.zucman-rossi@inserm.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay tag fluorescence tag visualisation by fluorescence tag fluorescence Pilati C., Letouzé E., Nault J.C., Imbeaud S., Boulai A., Calderaro J., Poussin K., Franconi A., Couchy G., Morcrette G., Mallet M., Taouji S., Balabaud C., Terris B., Canal F., Paradis V., Scoazec J.Y., de Muret A., Guettier C., Bioulac-Sage P., Chevet E., Calvo F., Zucman-Rossi J. Cancer - Interactions investigated in the context of cancer Should the artificial peptides be curated at all? the Omnia 5 peptide "EAEAIYA" gives no results on Blasting. I have done them anyway. Kinase assay using Omnia® Y Peptide 5 Kit, Life Technology. (see PMID:15782220). This assay measures the increase in fluorescence of a Sox (a synthetic amino acid) moiety, which accompanies the phosphorylation of a nearby Ser, Thr or Tyr residue. jessica.zucman-rossi@inserm.fr Accepted 2014-JUN-06 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Genomic Profiling of Hepatocellular Adenomas Reveals Recurrent FRK-Activating Mutations and the Mechanisms of Malignant Transformation. Genomic Profiling of Hepatocellular Adenomas Reveals Recurrent FRK-Activating Mutations and the Mechanisms of Malignant Transformation. Cancer Cell (1535-6108) 2014 imex curation Pilati C., Letouzé E., Nault J.C., Imbeaud S., Boulai A., Calderaro J., Poussin K., Franconi A., Couchy G., Morcrette G., Mallet M., Taouji S., Balabaud C., Terris B., Canal F., Paradis V., Scoazec J.Y., de Muret A., Guettier C., Bioulac-Sage P., Chevet E., Calvo F., Zucman-Rossi J. Cancer - Interactions investigated in the context of cancer Should the artificial peptides be curated at all? the Omnia 5 peptide "EAEAIYA" gives no results on Blasting. I have done them anyway. jessica.zucman-rossi@inserm.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay antibody detection identification by antibody antibody detection Pilati C., Letouzé E., Nault J.C., Imbeaud S., Boulai A., Calderaro J., Poussin K., Franconi A., Couchy G., Morcrette G., Mallet M., Taouji S., Balabaud C., Terris B., Canal F., Paradis V., Scoazec J.Y., de Muret A., Guettier C., Bioulac-Sage P., Chevet E., Calvo F., Zucman-Rossi J. Cancer - Interactions investigated in the context of cancer The Src Kinase inhibitor "SRC-Inhibitor-5" was also used in this assay system, but results have not been entered here as it has not been possible to further identify this molecule. Should the artificial peptides be curated at all? the Omnia 5 peptide "EAEAIYA" gives no results on Blasting. I have done them anyway. Kinase activity detected by AlphaScreen®-based Assay (Amplified Luminescent Proximity Homogeneous Assay, -MI:0905). In this experiment Anti-phosphotyrosine antibodies were conjugated to Donor beads and Streptavidin Acceptor beads were used. jessica.zucman-rossi@inserm.fr Accepted 2014-JUN-06 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Genomic Profiling of Hepatocellular Adenomas Reveals Recurrent FRK-Activating Mutations and the Mechanisms of Malignant Transformation. Genomic Profiling of Hepatocellular Adenomas Reveals Recurrent FRK-Activating Mutations and the Mechanisms of Malignant Transformation. Cancer Cell (1535-6108) 2014 imex curation Pilati C., Letouzé E., Nault J.C., Imbeaud S., Boulai A., Calderaro J., Poussin K., Franconi A., Couchy G., Morcrette G., Mallet M., Taouji S., Balabaud C., Terris B., Canal F., Paradis V., Scoazec J.Y., de Muret A., Guettier C., Bioulac-Sage P., Chevet E., Calvo F., Zucman-Rossi J. Cancer - Interactions investigated in the context of cancer Should the artificial peptides be curated at all? the Omnia 5 peptide "EAEAIYA" gives no results on Blasting. I have done them anyway. jessica.zucman-rossi@inserm.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hep_3b Homo sapiens hepatocellular carcinoma (HCC) cells (p53-null). hep_3b human hepatocellular carcinoma (HCC) cells (p53-null). confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Pilati C., Letouzé E., Nault J.C., Imbeaud S., Boulai A., Calderaro J., Poussin K., Franconi A., Couchy G., Morcrette G., Mallet M., Taouji S., Balabaud C., Terris B., Canal F., Paradis V., Scoazec J.Y., de Muret A., Guettier C., Bioulac-Sage P., Chevet E., Calvo F., Zucman-Rossi J. Cancer - Interactions investigated in the context of cancer Should the artificial peptides be curated at all? the Omnia 5 peptide "EAEAIYA" gives no results on Blasting. I have done them anyway. jessica.zucman-rossi@inserm.fr Accepted 2014-JUN-06 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation src_inhibitor_5 SRC Inhibitor 5 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) src_inhibitor_1 SRC Inhibitor 1 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pp2 PP2 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) frk_human Tyrosine-protein kinase FRK PTK5 RAK FYN-related kinase Nuclear tyrosine protein kinase RAK FRK Protein-tyrosine kinase 5 protein protein human Homo sapiens Human MSNICQRLWEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTAEDLSFRAGDKLQVLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAEDRSLQAEPWFFGAIGRSDAEKQLLYSENKTGSFLIRESESQKGEFSLSVLDGAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHYTKTSDGLCVKLGKPCLKIQVPAPFDLSYKTVDQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQIMKNLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNYIHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSNKFSIKSDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNCPQQFYNIMLECWNAEPKERPTFETLRWKLEDYFETDSSYSDANNFIR 06EC050DDBCD930B dasatinib Dasatinib small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) Protein kinase inhibitor Protein kinase inhibitor poly(glu:tyr) 4:1 Poly(Glu,Tyr) 4:1 random copolymer small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) omnia_y_peptide_5 Omnia Y Peptide 5 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EAEAIYA Ac-EAEAIYAAdP(Sox)G-NH2 stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D DIP protein P40763 original sequence version: 157 DIP protein P40763-1 original sequence version: 147 DIP protein P40763-1 original sequence version: 135 DIP protein P40763-1 original sequence version: 157 DIP protein P40763 original sequence version: 165 DIP protein P40763 original sequence version: 182 DIP protein P40763 original sequence version: 135 6C00632211C8012D frk-stat3 3 11 unspecified role unspecified role neutral component neutral component required_phosphotyr_705 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role neutral component neutral component undefined mutation mutation mutation substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:47:23 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'val378_lys380_del-ins_glu' This is a deletion of amino acids 378-380 and insertion at same site of a glutamine P42685:p.Glu346Gly mutation mutation deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain E G Wed May 02 17:36:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu346gly' Tue Jun 30 16:52:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu346Gly' P42685:p.Val378_Phe379del mutation mutation deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain VF .. Thu Jul 07 12:17:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val378_phe379-del Fri Jul 22 11:07:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val378del,phe379del' Wed May 02 16:03:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_phe378-379del_del' Tue Jun 30 19:00:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val378_Phe379del' c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2D frk-stat3-1 2 9 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme enzyme neutral component neutral component undefined mutation mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Wed Jul 06 15:06:14 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'val378_lys380_del-ins_glu' This is a deletion of amino acids 378-380 and insertion at same site of a glutamine P42685:p.Val378_Phe379del mutation increasing rate mutation increasing interaction rate mutation increasing rate deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain VF .. Thu Jul 07 12:17:19 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val378_phe379-del Fri Jul 22 10:54:39 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val378del,phe379del' Wed May 02 17:05:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_phe378-379del_del' Tue Jun 30 18:44:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val378_Phe379del' n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 2E poly(glu:tyr) 4:1 phosphotyr O-phospho-L-tyrosine FRK-WT Vmax =57 umol/mg/min, Km =8.2uM VF mutant Vmax =88 umol/mg/min, Km =3.1uM FK mutant Vmax =58 umol/mg/min, Km =4.5uM VF and FK FRK mutants exhibited an increased kinase activity compared with the wild-type FRK. frk-poly_glu_tyr__4_1-1 2 5 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme enzyme neutral component neutral component undefined mutation mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:45:51 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'val378_lys380_del-ins_glu' This is a deletion of amino acids 378-380 and insertion at same site of a glutamine n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P42685:p.Val378_Phe379del mutation increasing rate mutation increasing interaction rate mutation increasing rate deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain VF .. Thu Jul 07 12:18:18 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val378_phe379-del Fri Jul 22 11:12:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val378del,phe379del' Wed May 02 15:25:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_phe378-379del_del' Tue Jun 30 16:52:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val378_Phe379del' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 2F poly(glu:tyr) 4:1 phosphotyr O-phospho-L-tyrosine frk-poly_glu_tyr__4_1-2 2 7 enzyme enzyme neutral component neutral component undefined mutation mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:46:06 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'val378_lys380_del-ins_glu' This is a deletion of amino acids 378-380 and insertion at same site of a glutamine n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P42685:p.Val378_Phe379del mutation increasing rate mutation increasing interaction rate mutation increasing rate deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain VF .. Thu Jul 07 12:07:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val378_phe379-del Fri Jul 22 11:11:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val378del,phe379del' Wed May 02 17:21:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_phe378-379del_del' Tue Jun 30 18:24:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val378_Phe379del' ecoli Escherichia coli (strain K12) 6 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation 2F poly(glu:tyr) 4:1 phosphotyr O-phospho-L-tyrosine frk-poly_glu_tyr__4_1-3 2 8 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P42685:p.Val378_Phe379del mutation increasing rate mutation increasing interaction rate mutation increasing rate deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain VF .. Thu Jul 07 12:07:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val378_phe379-del Fri Jul 22 11:09:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val378del,phe379del' Wed May 02 15:52:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_phe378-379del_del' Tue Jun 30 18:33:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val378_Phe379del' undefined mutation mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:57:44 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'val378_lys380_del-ins_glu' This is a deletion of amino acids 378-380 and insertion at same site of a glutamine ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 2F poly(glu:tyr) 4:1 phosphotyr O-phospho-L-tyrosine frk-stat3-2 1 10 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus acglu N-acetyl-L-glutamic acid (2S)-2-(acetamido)pentanedioic acid 2-(acetylamino)pentanedioic acid 2-(acetylazanyl)pentanedioic acid MOD_RES N-acetylglutamate N-acetyl-L-glutamic acid N-acetylated L-glutamic acid acetylglutamic acid AcGlu n-term range n-terminal range n-term range n-term range n-terminal range n-term range adp_sox_glycine fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme enzyme neutral component neutral component P42685:p.Val378_Phe379del mutation increasing rate mutation increasing interaction rate mutation increasing rate deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain VF .. Thu Jul 07 12:07:19 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val378_phe379-del Fri Jul 22 10:49:46 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val378del,phe379del' Wed May 02 20:06:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_phe378-379del_del' Tue Jun 30 19:00:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val378_Phe379del' n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range mouse-ba_f3 Mouse pro B cells IL-3 dependent mouse-baf3 EBI-6922762 ba_f3 Mouse pro B cells IL-3 dependent BA/F3 baf3 phosphorylation phosphorylation reaction phosphorylation Supp 2D omnia_y_peptide_5 phosphotyr O-phospho-L-tyrosine VF FRK mutant exhibited an increased kinase activity compared with the wild-type FRK. frk-poly_glu_tyr__4_1-4 2 9 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme enzyme neutral component neutral component undefined mutation mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:02:08 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'val378_lys380_del-ins_glu' This is a deletion of amino acids 378-380 and insertion at same site of a glutamine n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P42685:p.Val378_Phe379del mutation increasing rate mutation increasing interaction rate mutation increasing rate deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain VF .. Thu Jul 07 12:07:31 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val378_phe379-del Fri Jul 22 10:52:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val378del,phe379del' Wed May 02 14:29:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_phe378-379del_del' Tue Jun 30 20:15:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val378_Phe379del' ecoli Escherichia coli (strain K12) 4 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation 2Fi poly(glu:tyr) 4:1 phosphotyr O-phospho-L-tyrosine