IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. Cancer Cell (1535-6108) 2014 imex curation Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Accepted 2014-AUG-05 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. Cancer Cell (1535-6108) 2014 imex curation Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro ubiquitinase assay ubiquitinase assay ubiquitinase assay anti tag western anti tag western blot anti tag western Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Accepted 2014-AUG-05 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. Cancer Cell (1535-6108) 2014 imex curation Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-caki_2 Human renal cell carcinoma line Caki2 caki_2 Renal cell carcinoma cell line caki-2 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Accepted 2014-AUG-05 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. Cancer Cell (1535-6108) 2014 imex curation Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro ubiquitinase assay ubiquitinase assay ubiquitinase assay western blot western blot Immuno blot Immuno blot Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Accepted 2014-AUG-05 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. Cancer Cell (1535-6108) 2014 imex curation Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-caki_2 Human renal cell carcinoma line Caki2 caki_2 Renal cell carcinoma cell line caki-2 ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Accepted 2014-AUG-05 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. Cancer Cell (1535-6108) 2014 imex curation Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer PTEN + DUSP constructs - my Data-Proc statement: not sure if this is good enough evidence to identify these constructs as Human -though they probably are.... All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Accepted 2014-AUG-05 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation hif1a_human Hypoxia-inducible factor 1-alpha ARNT-interacting protein Member of PAS protein 1 Basic-helix-loop-helix-PAS protein MOP1 HIF1A MOP1 BHLHE78 PASD8 PAS domain-containing protein 8 Class E basic helix-loop-helix protein 78 protein protein human Homo sapiens Human MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN DIP protein Q16665 original sequence version: 94 DIP protein Q16665 original sequence version: 98 DIP protein Q16665-1 original sequence version: 102 DIP protein Q16665 original sequence version: 102 DIP protein Q16665 original sequence version: 127 DIP protein Q16665 original sequence version: 120 DIP protein Q16665 original sequence version: 169 DIP protein Q16665 original sequence version: 174 DIP protein Q16665-1 original sequence version: 174 DIP protein Q16665-1 original sequence version: 191 DIP protein Q16665 original sequence version: 191 DIP protein Q16665 original sequence version: 179 DIP protein Q16665 original sequence version: 150 DIP protein Q16665 original sequence version: 156 DIP protein Q16665 original sequence version: 177 ABD4F7DAA135BE2D ub2d2_human Ubiquitin-conjugating enzyme E2 D2 UBC5B UBCH4 UBE2D2 UBC4 UBCH5B Ubiquitin-protein ligase D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-conjugating enzyme E2(17)KB 2 PUBC1 p53-regulated ubiquitin-conjugating enzyme 1 (E3-independent) E2 ubiquitin-conjugating enzyme D2 E2 ubiquitin-conjugating enzyme D2 protein protein human Homo sapiens Human MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM DIP protein P62837 original sequence version: 81 DIP protein P62837-1 original sequence version: 134 DIP protein P62837 original sequence version: 95 DIP protein P62837 original sequence version: 139 DIP protein P62837 original sequence version: 37 DIP protein P62837 original sequence version: 115 DIP protein P62837 original sequence version: 58 DIP protein P62837 original sequence version: 74 DIP protein P62837 original sequence version: 103 DIP protein P62837 original sequence version: 101 C942BE7853CBC355 pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 75F97C3DD6802BA9 spop_human_gene SPOP HUMAN GENE gene gene human Homo sapiens Human dus7_human Dual specificity protein phosphatase 7 DUSP7 PYST2 Dual specificity protein phosphatase PYST2 protein protein human Homo sapiens Human MKNQLRGPPARAHMSTSGAAAAGGTRAGSEPGAGSGSGAGTGAGAATGAGAMPCKSAEWLQEELEARGGASLLLLDCRPHELFESSHIETAINLAIPGLMLRRLRKGNLPIRSIIPNHADKERFATRCKAATVLLYDEATAEWQPEPGAPASVLGLLLQKLRDDGCQAYYLQGGFNKFQTEYSEHCETNVDSSSSPSSSPPTSVLGLGGLRISSDCSDGESDRELPSSATESDGSPVPSSQPAFPVQILPYLYLGCAKDSTNLDVLGKYGIKYILNVTPNLPNAFEHGGEFTYKQIPISDHWSQNLSQFFPEAISFIDEARSKKCGVLVHCLAGISRSVTVTVAYLMQKMNLSLNDAYDFVKRKKSNISPNFNFMGQLLDFERTLGLSSPCDNHASSEQLYFSTPTNHNLFPLNTLEST 434467AC16A3F8BA ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG uba1_human Ubiquitin-like modifier-activating enzyme 1 Ubiquitin-activating enzyme E1 Protein A1S9 UBA1 A1S9T UBE1 protein protein human Homo sapiens Human MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR DIP protein P22314 original sequence version: 157 DIP protein P22314 original sequence version: 152 4B413AAA75FAA562 spop_human Speckle-type POZ protein HIB homolog 1 Roadkill homolog 1 SPOP protein protein human Homo sapiens Human MSRVPSPPPPAEMSSGPVAESWCYTQIKVVKFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS DIP protein O43791 original sequence version: 150 EE5F4C5CF6FD09DC vegfa_human_gene Human VEGFA gene gene gene human Homo sapiens Human hif1a-spop 5 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey region_of_intron_1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1B hif1a-spop-1 3 10 unspecified role unspecified role prey prey region_of_intron_1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait association association Supp. 1A Q16665 increases expression at RNA level of ENSG00000121067 hypoxia Q16665 increases expression at protein level of ENSG00000121067 hif1a-spop-2 3 7 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. 1Ai hypoxic conditions Q16665 increases expression at protein level of ENSG00000112715 Q16665 increases expression at RNA level of ENSG00000112715 pten-spop 6 14 unspecified role unspecified role neutral component neutral component math_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component sbc_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 30 33 direct interaction direct interaction 4A, Supp 4B 2.0A Rwork =19.2 RFree =22.1 pten-spop-2 1 14 unspecified role unspecified role prey prey cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range sbcm1_motif required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4B spop-pten 1 9 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4C pten-spop-10 2 14 enzyme enzyme neutral component neutral component unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sbcm1_motif required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 13 unspecified role unspecified role ancillary ancillary 12 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination 4G, Supp 4C. P60484 ubiquitinylated lysine pten-spop-5 1 11 unspecified role unspecified role bait bait c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ser192-194gly required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 14 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5A spop-dusp7 1 11 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5B dusp7-spop-3 2 14 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 13 unspecified role unspecified role ancillary ancillary 12 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination 5F Q16829 ubiquitinylated lysine dusp7-spop-5 4 11 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser192-194gly required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 14 enzyme enzyme neutral component neutral component unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined possible tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role ancillary ancillary 12 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination Supp 5C Q16829 ubiquitinylated lysine Image text states that:- "Ubiquitination assay was performed as Figure 5H." It has been assumed that Figure 5F is intended here, as there is no Figure 5H. dusp7-spop-7 4 13 unspecified role unspecified role ancillary ancillary 12 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component region ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 14 enzyme enzyme neutral component neutral component possible tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination Q16829 ubiquitinylated lysine 5F, Supp. 5C.