IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Glycosylation-dependent lectin-receptor interactions preserve angiogenesis in anti-VEGF refractory tumors. Glycosylation-dependent lectin-receptor interactions preserve angiogenesis in anti-VEGF refractory tumors. Cell (0092-8674) 2014 imex curation Croci D.O., Cerliani J.P., Dalotto-Moreno T., Méndez-Huergo S.P., Mascanfroni I.D., Dergan-Dylon S., Toscano M.A., Caramelo J.J., García-Vallejo J.J., Ouyang J., Mesri E.A., Junttila M.R., Bais C., Shipp M.A., Salatino M., Rabinovich G.A. Cancer - Interactions investigated in the context of cancer gabyrabi@gmail.com Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Croci D.O., Cerliani J.P., Dalotto-Moreno T., Méndez-Huergo S.P., Mascanfroni I.D., Dergan-Dylon S., Toscano M.A., Caramelo J.J., García-Vallejo J.J., Ouyang J., Mesri E.A., Junttila M.R., Bais C., Shipp M.A., Salatino M., Rabinovich G.A. Cancer - Interactions investigated in the context of cancer Origin of Galectin-1 traced to PMID:12223272. Additional details on VGFR2 obtained from manfacturer's website. Construct could have either a His or an Fc tag at the C-terminus (both products are available). gabyrabi@gmail.com Accepted 2015-MAR-31 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Glycosylation-dependent lectin-receptor interactions preserve angiogenesis in anti-VEGF refractory tumors. Glycosylation-dependent lectin-receptor interactions preserve angiogenesis in anti-VEGF refractory tumors. Cell (0092-8674) 2014 imex curation Croci D.O., Cerliani J.P., Dalotto-Moreno T., Méndez-Huergo S.P., Mascanfroni I.D., Dergan-Dylon S., Toscano M.A., Caramelo J.J., García-Vallejo J.J., Ouyang J., Mesri E.A., Junttila M.R., Bais C., Shipp M.A., Salatino M., Rabinovich G.A. Cancer - Interactions investigated in the context of cancer gabyrabi@gmail.com Only protein-protein interactions human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Croci D.O., Cerliani J.P., Dalotto-Moreno T., Méndez-Huergo S.P., Mascanfroni I.D., Dergan-Dylon S., Toscano M.A., Caramelo J.J., García-Vallejo J.J., Ouyang J., Mesri E.A., Junttila M.R., Bais C., Shipp M.A., Salatino M., Rabinovich G.A. Cancer - Interactions investigated in the context of cancer gabyrabi@gmail.com Accepted 2015-MAR-31 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Glycosylation-dependent lectin-receptor interactions preserve angiogenesis in anti-VEGF refractory tumors. Glycosylation-dependent lectin-receptor interactions preserve angiogenesis in anti-VEGF refractory tumors. Cell (0092-8674) 2014 imex curation Croci D.O., Cerliani J.P., Dalotto-Moreno T., Méndez-Huergo S.P., Mascanfroni I.D., Dergan-Dylon S., Toscano M.A., Caramelo J.J., García-Vallejo J.J., Ouyang J., Mesri E.A., Junttila M.R., Bais C., Shipp M.A., Salatino M., Rabinovich G.A. Cancer - Interactions investigated in the context of cancer gabyrabi@gmail.com Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip tag fluorescence tag visualisation by fluorescence tag fluorescence Croci D.O., Cerliani J.P., Dalotto-Moreno T., Méndez-Huergo S.P., Mascanfroni I.D., Dergan-Dylon S., Toscano M.A., Caramelo J.J., García-Vallejo J.J., Ouyang J., Mesri E.A., Junttila M.R., Bais C., Shipp M.A., Salatino M., Rabinovich G.A. Cancer - Interactions investigated in the context of cancer HA-tagged VEGFR2 was stably expressed in HEK293T cells. Using anti-HA-conjugated 633-fluorescent beads, VEGFR2 was immunoprecipitated and binding of 488-Gal1 to immunoprecipitates examined by flow cytometry. Origin of Galectin-1 traced to PMID:12223272 gabyrabi@gmail.com Accepted 2015-MAR-31 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation vgfr2_human Vascular endothelial growth factor receptor 2 Kinase insert domain receptor Protein-tyrosine kinase receptor flk-1 KDR FLK1 Fetal liver kinase 1 VEGFR2 protein protein human Homo sapiens Human MQSKVLLAVALWLCVETRAASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGAQEKTNLEIIILVGTAVIAMFFWLLLVIILRTVKRANGGELKTGYLSIVMDPDELPLDEHCERLPYDASKWEFPRDRLKLGKPLGRGAFGQVIEADAFGIDKTATCRTVAVKMLKEGATHSEHRALMSELKILIHIGHHLNVVNLLGACTKPGGPLMVIVEFCKFGNLSTYLRSKRNEFVPYKTKGARFRQGKDYVGAIPVDLKRRLDSITSSQSSASSGFVEEKSLSDVEEEEAPEDLYKDFLTLEHLICYSFQVAKGMEFLASRKCIHRDLAARNILLSEKNVVKICDFGLARDIYKDPDYVRKGDARLPLKWMAPETIFDRVYTIQSDVWSFGVLLWEIFSLGASPYPGVKIDEEFCRRLKEGTRMRAPDYTTPEMYQTMLDCWHGEPSQRPTFSELVEHLGNLLQANAQQDGKDYIVLPISETLSMEEDSGLSLPTSPVSCMEEEEVCDPKFHYDNTAGISQYLQNSKRKSRPVSVKTFEDIPLEEPEVKVIPDDNQTDSGMVLASEELKTLEDRTKLSPSFGGMVPSKSRESVASEGSNQTSGYQSGYHSDDTDTTVYSSEEAELLKLIEIGVQTGSTAQILQPDSGTTLSSPPV DIP protein P35968 original sequence version: 81 DIP protein P35968 original sequence version: 146 DIP protein P35968 original sequence version: 117 DIP protein P35968 original sequence version: 121 59E7C44B05CFEBB3 leg1_human Galectin-1 Lectin galactoside-binding soluble 1 Beta-galactoside-binding lectin L-14-I S-Lac lectin 1 Galaptin 14 kDa lectin HPL HBL Putative MAPK-activating protein PM12 LGALS1 Lactose-binding lectin 1 14 kDa laminin-binding protein protein protein human Homo sapiens Human MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD DIP protein P09382 original sequence version: null DIP protein P09382 original sequence version: 111 2FBB8D7A1FC0F1F9 vgfr1_human Vascular endothelial growth factor receptor 1 Vascular permeability factor receptor Tyrosine-protein kinase receptor FLT Tyrosine-protein kinase FRT Fms-like tyrosine kinase 1 FLT1 FLT FRT VEGFR1 protein protein human Homo sapiens Human MVSYWDTGVLLCALLSCLLLTGSSSGSKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKMPTEGEDLKLSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIMNVSLQDSGTYACRARNVYTGEEILQKKEITIRDQEAPYLLRNLSDHTVAISSSTTLDCHANGVPEPQITWFKNNHKIQQEPGIILGPGSSTLFIERVTEEDEGVYHCKATNQKGSVESSAYLTVQGTSDKSNLELITLTCTCVAATLFWLLLTLFIRKMKRSSSEIKTDYLSIIMDPDEVPLDEQCERLPYDASKWEFARERLKLGKSLGRGAFGKVVQASAFGIKKSPTCRTVAVKMLKEGATASEYKALMTELKILTHIGHHLNVVNLLGACTKQGGPLMVIVEYCKYGNLSNYLKSKRDLFFLNKDAALHMEPKKEKMEPGLEQGKKPRLDSVTSSESFASSGFQEDKSLSDVEEEEDSDGFYKEPITMEDLISYSFQVARGMEFLSSRKCIHRDLAARNILLSENNVVKICDFGLARDIYKNPDYVRKGDTRLPLKWMAPESIFDKIYSTKSDVWSYGVLLWEIFSLGGSPYPGVQMDEDFCSRLREGMRMRAPEYSTPEIYQIMLDCWHRDPKERPRFAELVEKLGDLLQANVQQDGKDYIPINAILTGNSGFTYSTPAFSEDFFKESISAPKFNSGSSDDVRYVNAFKFMSLERIKTFEELLPNATSMFDDYQGDSSTLLASPMLKRFTWTDSKPKASLKIDLRVTSKSKESGLSDVSRPSFCHSSCGHVSEGKRRFTYDHAELERKIACCSPPPDYNSVVLYSTPPI FF3381EEFAF0787C flt1-lgals1-1 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 2E, 2G Gal1 associated with VEGFR2 through an N-glycosylation-dependent mechanism. Low levels of alpha2-6-linked sialic acid were detected. flt1-kdr-1 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. 2G High levels of alpha2-6-linked sialic acid were detected. kdr-lgals1-1 1 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region alexa 594 label alexa 594 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role fluorescence donor fluorescence donor region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined extracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human direct interaction direct interaction Fig. 2F, Supp. Fig. 2I Titration of the Gal1-VEGFR2 complex with lactose confirmed the glycan dependence of the interaction showing a considerable increase of 488-VEGFR2 fluorescence, which displayed a hyperbolic behavior and revealed an apparent Kd of 250 uM kdr-lgals1-3 3 4 unspecified role unspecified role bait bait P35968:p.Asn245Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Wed May 02 18:17:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn245gln' Tue Jun 30 18:19:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn245Gln' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35968:p.Asn318Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Wed May 02 18:41:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn318gln' Tue Jun 30 18:38:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn318Gln' P35968:p.Asn374Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Wed May 02 18:59:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn374gln' Tue Jun 30 17:33:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn374Gln' P35968:p.Asn704Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Wed May 02 15:46:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn704gln' Tue Jun 30 19:34:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn704Gln' P35968:p.Asn721Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Wed May 02 15:56:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn721gln' Tue Jun 30 19:37:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn721Gln' P35968:p.Asn675Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Wed May 02 15:50:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn675gln' Tue Jun 30 18:15:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn675Gln' P35968:p.Asn395Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Q Wed May 02 18:30:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn395gln' Tue Jun 30 16:50:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn395Gln' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role prey prey region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 2H Titration of the Gal1-VEGFR2 complex with lactose confirmed the glycan dependence of the interaction showing a considerable increase of 488-VEGFR2 fluorescence, which displayed a hyperbolic behavior and revealed an apparent Kd of 250 uM