IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. Cancer Cell (1535-6108) 2014 imex curation Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca Accepted 2014-APR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. Cancer Cell (1535-6108) 2014 imex curation Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip anti tag western anti tag western blot anti tag western Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer It is not stated which antibodies were used in this IP- both an anti-Pyo tag antibody and ant-KSR1 antibody are mentioned in this publication. It has been assumed an anti-tag antibody has been used to detect egfp-GEF-H1. The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca Accepted 2014-APR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. Cancer Cell (1535-6108) 2014 imex curation Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange tag visualisation tag visualisation Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer A real-time NMR-based assay to monitor hydrolysis or exchange of native nucleotides by recording nucleotide-dependent structural changes in a 15N- labeled GTPase protein over time. The unambiguous readout allows real-time assay of the kinetics of nucleotide exchange or hydrolysis performed in a complex mixture of proteins, including mammalian cell extracts. The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca Accepted 2014-APR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. Cancer Cell (1535-6108) 2014 imex curation Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca Accepted 2014-APR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. Cancer Cell (1535-6108) 2014 imex curation Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer Lysates derived from HRASV12-transformed NIH 3T3 cells. The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca Accepted 2014-APR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1. Cancer Cell (1535-6108) 2014 imex curation Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca 12-May-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Cullis J., Meiri D., Sandi M.J., Radulovich N., Kent O.A., Medrano M., Mokady D., Normand J., Larose J., Marcotte R., Marshall C.B., Ikura M., Ketela T., Moffat J., Neel B.G., Gingras A.C., Tsao M.S., Rottapel R. Cancer - Interactions investigated in the context of cancer The authors have stated that the GDF-H1 constructs used in these experiments is Murine in origin, and that the FLAG-PP2As are Human in origin. rottapel@uhnresearch.ca Accepted 2014-APR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation ksr1_human Kinase suppressor of Ras 1 KSR1 KSR protein protein human Homo sapiens Human MDRAALRAAAMGEKKEGGGGGDAAAAEGGAGAAASRALQQCGQLQKLIDISIGSLRGLRTKCAVSNDLTQQEIRTLEAKLVRYICKQRQCKLSVAPGERTPELNSYPRFSDWLYTFNVRPEVVQEIPRDLTLDALLEMNEAKVKETLRRCGASGDECGRLQYALTCLRKVTGLGGEHKEDSSWSSLDARRESGSGPSTDTLSAASLPWPPGSSQLGRAGNSAQGPRSISVSALPASDSPTPSFSEGLSDTCIPLHASGRLTPRALHSFITPPTTPQLRRHTKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVSSMRFDLSHGSPQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIVREMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIGSGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLNRRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM DIP protein Q8IVT5 original sequence version: 115 61BF0F1598349540 2aba_human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform PPP2R2A PP2A subunit B isoform alpha PP2A subunit B isoform B55-alpha PP2A subunit B isoform PR55-alpha PP2A subunit B isoform R2-alpha protein protein human Homo sapiens Human MAGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQENKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN DIP protein P63151 original sequence version: 33 DIP protein P63151 original sequence version: 66 F4D407FF7ADA4ED6 2a5b_human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform PPP2R5B PP2A B subunit isoform B'-beta PP2A B subunit isoform B56-beta PP2A B subunit isoform PR61-beta PP2A B subunit isoform R5-beta protein protein human Homo sapiens Human METKLPPASTPTSPSSPGLSPVPPPDKVDGFSRRSLRRARPRRSHSSSQFRYQSNQQELTPLPLLKDVPASELHELLSRKLAQCGVMFDFLDCVADLKGKEVKRAALNELVECVGSTRGVLIEPVYPDIIRMISVNIFRTLPPSENPEFDPEEDEPNLEPSWPHLQLVYEFFLRFLESPDFQPSVAKRYVDQKFVLMLLELFDSEDPREREYLKTILHRVYGKFLGLRAYIRKQCNHIFLRFIYEFEHFNGVAELLEILGSIINGFALPLKTEHKQFLVRVLIPLHSVKSLSVFHAQLAYCVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEMEEILDVIEPSQFVKIQEPLFKQVARCVSSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAVFGTLYQVSKEHWNQTIVSLIYNVLKTFMEMNGKLFDELTASYKLEKQQEQQKAQERQELWQGLEELRLRRLQGTQGAKEAPLQRLTPQVAASGGQS 8BEF84F20A77982D rhoa_mouse Transforming protein RhoA Rhoa Arha Arha2 protein protein mouse Mus musculus Mouse MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLIL DIP protein Q9QUI0 original sequence version: 76 DIP protein Q9QUI0 original sequence version: 77 DIP protein Q9QUI0 original sequence version: 100 C4C8BDC31FF858BC rtkn_human Rhotekin RTKN1 RTKN protein protein human Homo sapiens Human MFSRNHRSRVTVARGSALEMEFKRGRFRLSLFSDLPEDTELQRKLDHEIRMREGACKLLAACSQREQALEATKSLLVCNSRILSYMGELQRRKEAQVLGKTSRRPSDSGPPAERSPCRGRVCISDLRIPLMWKDTEYFKNKGDLHRWAVFLLLQLGEHIQDTEMILVDRTLTDISFQSNVLFAEAGPDFELRLELYGACVEEEGALTGGPKRLATKLSSSLGRSSGRRVRASLDSAGGSGSSPILLPTPVVGGPRYHLLAHTTLTLAAVQDGFRTHDLTLASHEENPAWLPLYGSVCCRLAAQPLCMTQPTASGTLRVQQAGEMQNWAQVHGVLKGTNLFCYRQPEDADTGEEPLLTIAVNKETRVRAGELDQALGRPFTLSISNQYGDDEVTHTLQTESREALQSWMEALWQLFFDMSQWKQCCDEIMKIETPAPRKPPQALAKQGSLYHEMAIEPLDDIAAVTDILTQREGARLETPPPWLAMFTDQPALPNPCSPASVAPAPDWTHPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKGQPRTWLQSPV DIP protein Q9BST9 original sequence version: 112 B3C64CB737E88501 2a5e_human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform PPP2R5E PP2A B subunit isoform B'-epsilon PP2A B subunit isoform B56-epsilon PP2A B subunit isoform PR61-epsilon PP2A B subunit isoform R5-epsilon protein protein human Homo sapiens Human MSSAPTTPPSVDKVDGFSRKSVRKARQKRSQSSSQFRSQGKPIELTPLPLLKDVPSSEQPELFLKKLQQCCVIFDFMDTLSDLKMKEYKRSTLNELVDYITISRGCLTEQTYPEVVRMVSCNIFRTLPPSDSNEFDPEEDEPTLEASWPHLQLVYEFFIRFLESQEFQPSIAKKYIDQKFVLQLLELFDSEDPRERDYLKTVLHRIYGKFLGLRAFIRKQINNIFLRFVYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLVKVLIPLHTVRSLSLFHAQLAYCIVQFLEKDPSLTEPVIRGLMKFWPKTCSQKEVMFLGELEEILDVIEPSQFVKIQEPLFKQIAKCVSSPHFQVAERALYYWNNEYIMSLIEENSNVILPIMFSSLYRISKEHWNPAIVALVYNVLKAFMEMNSTMFDELTATYKSDRQREKKKEKEREELWKKLEDLELKRGLRRDGIIPT DD9CE11433F499CF pp2ab_human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform PPP2CB protein protein human Homo sapiens Human MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL DIP protein P62714 original sequence version: 117 51DA9EB0633FC191 2a5a_human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform PP2A B subunit isoform B'-alpha PP2A B subunit isoform B56-alpha PP2A B subunit isoform PR61-alpha PP2A B subunit isoform R5-alpha PPP2R5A protein protein human Homo sapiens Human MSSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE D31407F7032A6D44 arhg2_human Rho guanine nucleotide exchange factor 2 ARHGEF2 KIAA0651 LFP40 Guanine nucleotide exchange factor H1 Microtubule-regulated Rho-GEF Proliferating cell nucleolar antigen p40 protein protein human Homo sapiens Human MSRIESLTRARIDRSRELASKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNMRNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIETEDEAYLRRIKMELQQKDRALVELLREKVGLFAEMTHFQAEEDGGSGMALPTLPRGLFRSESLESPRGERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHSPRDFTRMQDIPEETESRDGEAVASES 8986AF0A1174EA71 2a5e_mouse Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform Ppp2r5e Kiaa4006 PP2A B subunit isoform B'-epsilon PP2A B subunit isoform B56-epsilon PP2A B subunit isoform PR61-epsilon PP2A B subunit isoform R5-epsilon protein protein mouse Mus musculus Mouse MSSAPTTPPSVDKVDGFSRKSVRKARQKRSQSSSQFRSQGKPIELTPLPLLKDVPTSEQPELFLKKLQQCCVIFDFMDTLSDLKMKEYKRSTLNELVDYITISRGCLTEQTYPEVVRMVSCNIFRTLPPSDSNEFDPEEDEPTLEASWPHLQLVYEFFIRFLESQEFQPSIAKKYIDQKFVLQLLELFDSEDPRERDYLKTVLHRIYGKFLGLRAFIRKQINNIFLRFVYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLVKVLIPLHTVRSLSLFHAQLAYCIVQFLEKDPSLTEPVIRGLMKFWPKTCSQKEVMFLGELEEILDVIEPSQFVKIQEPLFKQIAKCVSSPHFQVAERALYYWNNEYIMSLIEENSNVILPIMFSSLYRISKEHWNPAIVALVYNVLKAFMEMNSTMFDELTATYKSDRQREKKKEKEREELWKKLEDLELKRGLRRDGIIPT mint The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Interacts with cyclin G in vitro 77DA129F9A4EC6AC arhg2_mouse Rho guanine nucleotide exchange factor 2 Kiaa0651 Lbcl1 Lfc Guanine nucleotide exchange factor H1 Lymphoid blast crisis-like 1 LBC'S first cousin Oncogene LFC Rhobin Arhgef2 protein protein mouse Mus musculus Mouse MSRIESLTRARIDRSKEQATKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLRNNTALQSVSLRSKTTTRERPTSAIYPSDSFRQSLLGSRRGLSSLSLAKSVSTTNIAGHFNDESPLGLRQILSQSTDSLNMRNRTLSVESLIDEGVEVFYNELMSDFEMDEKDFEADSWSLAVDSSFLQQHKKEVMKKQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQMEPEVVQGLFPCVDELSDIHTRFLNQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGSNAEQMRKTYSEFCSRHTKALKLYKELYARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLINRILQNSHGVEEEYQDLASALGLVKELLSNVDQDVHELEKEARLQEIYNRMDPRAQTPVPGKGPFGRDELLRRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPSVVSLQNLIVRDIANQAKGMFLISSGPPEMYEVHAASRDDRTTWIRVIQQSVRLCPSREDFPLIETEDKAYLRRIKTKLQQKNQALVELLQKNVELFAEMVHFQALKAGFVGMPPPALPRGLFRLESFESLRGERLLKDALREVEGLKDLLLGPCVDLPMTSREPALPLDSDSGSCPGVTANGEARTFNGSIELCRADSDSSQKDRNGNQLRSPQEEVLQPLINLYGLLHGLQAVVVQQERLMEALFPEGPERWEKLSRANSRDGEAGRAAVASVTPEKQATELALLQRQHTLLQEELRRCQRLGEERATEAGSLEARLRESEQARALLEREAEEIRRQLAALGQNEPLPAEAPWARRPLDPRRRSLPAGDALYLSFNPPQPSRGHDRLDLPVTVRSLHRPFDDREAQELGSPEDRLQDSSDPDTGSEEEVSSRLSPPHSPRDFTRMQDIPEETESRDGEPTASES 1A5EAB73F95005D1 rhoa_human Transforming protein RhoA Rho cDNA clone 12 ARHA RHO12 RHOA ARH12 protein protein human Homo sapiens Human MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL DIP protein P61586 original sequence version: 86 DIP protein P61586 original sequence version: 123 DIP protein P61586 original sequence version: 57 DIP protein P61586 original sequence version: 88 DIP protein P61586 original sequence version: 87 DIP protein P61586 original sequence version: 85 DIP protein P61586 original sequence version: 125 DIP protein P61586 original sequence version: 132 C4DA2DC31FF858BC q61210-5 Rho guanine nucleotide exchange factor 1 Arhgef1 Lbcl2 Lsc Lbc's second cousin Lymphoid blast crisis-like 2 protein protein mouse Mus musculus Mouse MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANSEDQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEVVQSQQAAVSRQLEDFRSKRLMGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEETQHTISTDEEKSAAVVTAISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGEPSAPDCRHLKVEADEKPGPADRKGGLGMSSRDRTVGTPGQDNPGVSLHPLSTDSVDSREPGVDTPQEPGDTPPQGPTSLEPLAPPESTEDNGETERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPGSATAVLGPTRRATPEPGDDGEPGRSGLELEPEEPPGWRELVPPDTLLSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMADGGFFPLDELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESTERGKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAIEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTSSERKNWCNLITETAGSLKVPAPASRLKPRPSPSSIREPLLSSSENGTGGAEMAPADARTERLLNDLLPFCRPGPEGQLAATALQKVLSLKQILLSTEEDSGAGPPRDGDGVPGGRAPGPVHTQEIEENLLSLEVAIRQLEELEEEFCRLRPLLSQLGGTLSPNLAAPERSAQTGLS Contains a phosphoserine at position 386. Contains a phosphoserine at position 390. Contains a phosphoserine at position 412. Contains a phosphothreonine at position 432. FEF62EF944FFABD6 gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 ksr1_mouse Kinase suppressor of Ras 1 Ksr1 Ksr Protein Hb protein protein mouse Mus musculus Mouse MDRAALRAAAMGEKKEGGGGGAAADGGAGAAVSRALQQCGQLQKLIDISIGSLRGLRTKCSVSNDLTQQEIRTLEAKLVKYICKQQQSKLSVTPSDRTAELNSYPRFSDWLYIFNVRPEVVQEIPQELTLDALLEMDEAKAKEMLRRWGASTEECSRLQQALTCLRKVTGLGGEHKMDSGWSSTDARDSSLGPPMDMLSSLGRAGASTQGPRSISVSALPASDSPVPGLSEGLSDSCIPLHTSGRLTPRALHSFITPPTTPQLRRHAKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVTPMKFELPHGSPQLVRRDIGLSVTHRFSTKSWLSQVCNVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRITFLPLARLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNLDSSSNPSSTTSSTPSSPAPFLTSSNPSSATTPPNPSPGQRDSRFSFPDISACSQAAPLSSTADSTRLDDQPKTDVLGVHEAEAEEPEAGKSEAEDDEEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREERRENQLKLSHDWLCYLAPEIVREMIPGRDEDQLPFSKAADVYAFGTVWYELQARDWPFKHQPAEALIWQIGSGEGVRRVLASVSLGKEVGEILSACWAFDLQERPSFSLLMDMLERLPKLNRRLSHPGHFWKSADINSSKVMPRFERFGLGTLESGNPKM EAEEB23FAE715D94 arhgef2-ksr1 4 15 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 5C PDGF arhgef2-ksr1-1 1 21 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association Supp 3D PDGF ksr1-arhgef2-1 1 16 unspecified role unspecified role prey prey 21 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey association association Supp 5 PDGF arhgef2-gtp-1 3 18 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component Q60875:p.Glu243Lys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:09:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu243lys' Tue Jun 30 18:32:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu243Lys' n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 4B arhgef2-rhoa 3 18 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 19 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 4Bi arhgef2-ksr1-2 6 17 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 21 unspecified role unspecified role prey prey region pyo tag pyo tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4D ppp2cb-arhgef2 6 7 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 6A ppp2r5a-ksr1 6 15 unspecified role unspecified role ancillary ancillary 7 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 6Ai/D The interaction between KSR-1 and PP2A is dependent on GEF-H1. ppp2r5b-ksr1 6 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 15 unspecified role unspecified role ancillary ancillary association association 6Aii/D ppp2r5e-ksr1 6 15 unspecified role unspecified role ancillary ancillary 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 6Aiii/D ppp2r2a-ksr1 6 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 15 unspecified role unspecified role ancillary ancillary association association 6D The interaction between KSR-1 and PP2A is dependent on GEF-H1. arhgef2-ksr1-3 6 21 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range dh_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 6C PP2Ac was also present in all complexes, but due to lack of antibody specificity this molecule(s) cannot be identified. rtkn-rhoa-13 5 20 unspecified role unspecified role ancillary ancillary whole_molecule binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role prey prey n_terminal_gtp_binding_region sufficient to bind sufficient binding region sufficient to bind range range range range mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells 11 unspecified role unspecified role bait bait rb_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 85 83 physical association physical association Supp 3A Rhotekin-RBD only binds to GTP bound RhoA. No change was observed in Rho-GTP binding when GEF-H1 was knocked down. ksr1-arhgef2-2 2 17 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range [1-86], [152-985] sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 21 unspecified role unspecified role bait bait region pyo tag pyo tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 3E