IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Cancer Cell (1535-6108) 2013 imex curation Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu Accepted 2014-JAN-28 by ORCHARD Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Cancer Cell (1535-6108) 2013 imex curation Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu Accepted 2014-JAN-28 by ORCHARD Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Cancer Cell (1535-6108) 2013 imex curation Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu Accepted 2014-JAN-28 by ORCHARD Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Direct Reversal of Glucocorticoid Resistance by AKT Inhibition in Acute Lymphoblastic Leukemia. Cancer Cell (1535-6108) 2013 imex curation Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Piovan E., Yu J., Tosello V., Herranz D., Ambesi-Impiombato A., Da Silva A.C., Sanchez-Martin M., Perez-Garcia A., Rigo I., Castillo M., Indraccolo S., Cross J.R., de Stanchina E., Paietta E., Racevskis J., Rowe J.M., Tallman M.S., Basso G., Meijerink J.P., Cordon-Cardo C., Califano A., Ferrando A.A. Cancer - Interactions investigated in the context of cancer Colocalisation results have not been included due to lack of specificity of the "AKT" antibody. I have deleted piovan-2013-5 (must have forgotten) The authors have stated that HA/Flag/ GST/ NR3C1 and the S134A mutant were of human origin. Flag AKT1 and MYR- AKT1 vectors were from Addgene and are also human, the MYR-AKT1 construct encodes full-length human AKT1. ac2248@columbia.edu,af2196@columbia.edu Accepted 2014-JAN-28 by ORCHARD Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation gcr_human Glucocorticoid receptor Nuclear receptor subfamily 3 group C member 1 NR3C1 GRL protein protein human Homo sapiens Human MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMRPDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK C5C90C9A5DD16AAB akt1_human RAC-alpha serine/threonine-protein kinase AKT1 PKB RAC RAC-PK-alpha Protein kinase B Proto-oncogene c-Akt Protein kinase B alpha protein protein human Homo sapiens Human MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA DIP protein P31749 original sequence version: 184 DIP protein P31749 original sequence version: 168 DIP protein P31749 original sequence version: 102 DIP protein P31749 original sequence version: 110 DIP protein P31749 original sequence version: 160 DIP protein P31749 original sequence version: 136 DIP protein P31749 original sequence version: 175 DIP protein P31749 original sequence version: 201 DIP protein P31749 original sequence version: 166 DIP protein P31749 original sequence version: 178 DIP protein P31749-1 original sequence version: 194 DIP protein P31749 original sequence version: 106 DIP protein P31749 original sequence version: 141 6EAFF4F8AD436714 akt1-nr3c1-1 2 5 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B nr3c1-akt1 2 5 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range possible_phospho_ser473 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain physical association physical association 1A nr3c1-akt1-2 4 5 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P04150:p.Ser134Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:18:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser134ala' Tue Jun 30 18:20:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser134Ala' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. direct interaction direct interaction 1E nr3c1-akt1-3 1 5 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey possible_phospho_308thr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain n_terminus myrres myristoylated residue C14:0 aliphatic acylated residue MyrRes Myristoyl Myristoylation n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1G The AKT phospho-motif in the NR3C1 protein was not phosphorylated using cells expressing the HA-NR3C1 S134A mutant. akt1-nr3c1-2 3 5 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P04150:p.Ser134Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:28:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser134ala' Tue Jun 30 19:58:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser134Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation 1H In figure 1H AKT1 is "activated"- from figure 1A, this may mean it has been phosphorylated at serine 473. M+M says NR3C1 has a Flag as well as a GST tag. P04150 ser-134 O-phospho-L-serine P31749 decreases activity of P04150 akt1-1 3 6 putative self putative self putative self putative self n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation true 1H In figure 1H AKT1 is "activated"- from figure 1A, this may mean it has been phosphorylated at serine 473.