IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). The Journal of biological chemistry 2013 imex curation Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. Accepted 2020-FEB-19 AT 10:08 GMT AT 10:08 GMT by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). The Journal of biological chemistry 2013 imex curation Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. Accepted 2020-FEB-19 AT 10:08 GMT AT 10:08 GMT by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). The Journal of biological chemistry 2013 imex curation Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. Accepted 2020-FEB-19 AT 10:08 GMT AT 10:08 GMT by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). The Journal of biological chemistry 2013 imex curation Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. Accepted 2020-FEB-19 AT 10:08 GMT AT 10:08 GMT by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). The Journal of biological chemistry 2013 imex curation Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. Accepted 2020-FEB-19 AT 10:08 GMT AT 10:08 GMT by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). Leucine-rich repeat and immunoglobulin domain-containing protein-1 (Lrig1) negative regulatory action toward ErbB receptor tyrosine kinases is opposed by leucine-rich repeat and immunoglobulin domain-containing protein 3 (Lrig3). The Journal of biological chemistry 2013 imex curation Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. Accepted 2020-FEB-19 AT 10:08 GMT AT 10:08 GMT by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV DIP protein P04626 original sequence version: 198 DIP protein P04626 original sequence version: 122 DIP protein P04626 original sequence version: 206 DIP protein P04626 original sequence version: 195 DIP protein P04626 original sequence version: 150 DIP protein P04626 original sequence version: 179 DIP protein P04626 original sequence version: 146 DIP protein P04626 original sequence version: 147 39E9DFDA04DCF962 lrig3_human Leucine-rich repeats and immunoglobulin-like domains protein 3 LRIG3 LIG3 UNQ287/PRO326/PRO335 protein protein human Homo sapiens Human MSAPSLRARAAGLGLLLCAVLGRAGRSDSGGRGELGQPSGVAAERPCPTTCRCLGDLLDCSRKRLARLPEPLPSWVARLDLSHNRLSFIKASSMSHLQSLREVKLNNNELETIPNLGPVSANITLLSLAGNRIVEILPEHLKEFQSLETLDLSSNNISELQTAFPALQLKYLYLNSNRVTSMEPGYFDNLANTLLVLKLNRNRISAIPPKMFKLPQLQHLELNRNKIKNVDGLTFQGLGALKSLKMQRNGVTKLMDGAFWGLSNMEILQLDHNNLTEITKGWLYGLLMLQELHLSQNAINRISPDAWEFCQKLSELDLTFNHLSRLDDSSFLGLSLLNTLHIGNNRVSYIADCAFRGLSSLKTLDLKNNEISWTIEDMNGAFSGLDKLRRLILQGNRIRSITKKAFTGLDALEHLDLSDNAIMSLQGNAFSQMKKLQQLHLNTSSLLCDCQLKWLPQWVAENNFQSFVNASCAHPQLLKGRSIFAVSPDGFVCDDFPKPQITVQPETQSAIKGSNLSFICSAASSSDSPMTFAWKKDNELLHDAEMENYAHLRAQGGEVMEYTTILRLREVEFASEGKYQCVISNHFGSSYSVKAKLTVNMLPSFTKTPMDLTIRAGAMARLECAAVGHPAPQIAWQKDGGTDFPAARERRMHVMPEDDVFFIVDVKIEDIGVYSCTAQNSAGSISANATLTVLETPSFLRPLLDRTVTKGETAVLQCIAGGSPPPKLNWTKDDSPLVVTERHFFAAGNQLLIIVDSDVSDAGKYTCEMSNTLGTERGNVRLSVIPTPTCDSPQMTAPSLDDDGWATVGVVIIAVVCCVVGTSLVWVVIIYHTRRRNEDCSITNTDETNLPADIPSYLSSQGTLADRQDGYVSSESGSHHQFVTSSGAGFFLPQHDSSGTCHIDNSSEADVEAATDLFLCPFLGSTGPMYLKGNVYGSDPFETYHTGCSPDPRTVLMDHYEPSYIKKKECYPCSHPSEESCERSFSNISWPSHVRKLLNTSYSHNEGPGMKNLCLNKSSLDFSANPEPASVASSNSFMGTFGKALRRPHLDAYSSFGQPSDCQPRAFYLKAHSSPDLDSGSEEDGKERTDFQEENHICTFKQTLENYRTPNFQSYDLDT EB3B6A391F3C8713 lrig1_human Leucine-rich repeats and immunoglobulin-like domains protein 1 LRIG1 LIG1 protein protein human Homo sapiens Human MARPVRGGLGAPRRSPCLLLLWLLLLRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATGHPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKS 7B4F13CA343AF943 lrig3-lrig1-1 4 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd_region required to bind necessary binding region required to bind range range range range myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 9C lrig3-lrig1-2 5 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd_region required to bind necessary binding region required to bind range range range range 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 11C lrig1-lrig3-1 5 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain association association Figure 4 erbb2-lrig1-1 5 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4 Lrig3 (Q6UXM1) The amount of Lrig1 precipitating with ErbB2 reproducibly decreased when Lrig3 was co-expressed. lrig3-lrig1-3 2 8 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 6B lrig3-lrig1-4 6 8 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 6C lrig3-lrig1-5 1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6D lrig3-lrig1-6 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6D lrig1-lrig3-2 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6E lrig3-lrig1-7 4 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain ecd_region required to bind necessary binding region required to bind range range range range physical association physical association Figure 10C