IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Cancer Cell (1535-6108) 2013 imex curation Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu Accepted 2013-AUG-09 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Cancer Cell (1535-6108) 2013 imex curation Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu Accepted 2013-AUG-09 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Cancer Cell (1535-6108) 2013 imex curation Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Li M., Yu X. Cancer - Interactions investigated in the context of cancer Participant detection method found from Soutoglou et al., 2007. GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu Accepted 2013-AUG-09 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Cancer Cell (1535-6108) 2013 imex curation Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down antibody detection identification by antibody antibody detection Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu Accepted 2013-AUG-09 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Cancer Cell (1535-6108) 2013 imex curation Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu Accepted 2013-AUG-09 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Function of BRCA1 in the DNA Damage Response Is Mediated by ADP-Ribosylation. Cancer Cell (1535-6108) 2013 imex curation Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li M., Yu X. Cancer - Interactions investigated in the context of cancer GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. GST-BARD1 and GST-BRCA1 have been determined to be of human origin due to length of the molecules and mutation mapping. xiayu@umich.edu Accepted 2013-AUG-09 by PPORRAS 09-Sep-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation bard1_human BRCA1-associated RING domain protein 1 BARD1 RING-type E3 ubiquitin transferase BARD1 protein protein human Homo sapiens Human MPDNRQPRNRQPRIRSGNEPRSAPAMEPDGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGTGCPVCYTPAWIQDLKINRQLDSMIQLCSKLRNLLHDNELSDLKEDKPRKSLFNDAGNKKNSIKMWFSPRSKKVRYVVSKASVQTQPAIKKDASAQQDSYEFVSPSPPADVSERAKKASARSGKKQKKKTLAEINQKWNLEAEKEDGEFDSKEESKQKLVSFCSQPSVISSPQINGEIDLLASGSLTESECFGSLTEVSLPLAEQIESPDTKSRNEVVTPEKVCKNYLTSKKSLPLENNGKRGHHNRLSSPISKRCRTSILSTSGDFVKQTVPSENIPLPECSSPPSCKRKVGGTSGRKNSNMSDEFISLSPGTPPSTLSSSSYRRVMSSPSAMKLLPNMAVKRNHRGETLLHIASIKGDIPSVEYLLQNGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKNGHVDIVKLLLSYGASRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSASHCSVMNTGQRRDGPLVLIGSGLSSEQQKMLSELAVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCMLGILNGCWILKFEWVKACLRRKVCEQEEKYEIPEGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGKVWKAPSSWFIDCVMSFELLPLDS DIP protein Q99728 original sequence version: 118 95E2D904046B5646 brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY DIP protein P38398 original sequence version: 183 DIP protein P38398 original sequence version: 111 DIP protein P38398 original sequence version: 149 DIP protein P38398-1 original sequence version: 149 DIP protein P38398 original sequence version: 142 DIP protein P38398 original sequence version: 212 DIP protein P38398 original sequence version: 163 DIP protein P38398 original sequence version: 185 DIP protein P38398 original sequence version: 148 DIP protein P38398 original sequence version: 210 89C6D83FF56312AF adp-ribose ADP-D-ribose small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) par poly-ADP-D-ribose poly a poly adenine poly A poly a chemical synthesis Chemical synthesis (Chemical synthesis) bard1-par 4 7 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99728:p.Cys645Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C R Wed May 02 20:28:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys645arg' Tue Jun 30 17:58:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys645Arg' Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:42:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 17:14:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' Q99728:p.Val695Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V L Wed May 02 20:15:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val695leu' Tue Jun 30 16:20:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val695Leu' ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2A, S2A, S2C, S2D bard1-par-2 2 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:37:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 16:54:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction 2C bard1-par-3 2 7 unspecified role unspecified role neutral component neutral component Q99728:p.Val695Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V L Wed May 02 16:31:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val695leu' Tue Jun 30 18:52:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val695Leu' Q99728:p.Cys645Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C R Wed May 02 17:58:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys645arg' Tue Jun 30 16:51:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys645Arg' n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:59:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 19:52:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2D bard1-adp_ribose-1 1 7 unspecified role unspecified role bait bait Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:05:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 18:32:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' Q99728:p.Cys645Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C R Wed May 02 17:10:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys645arg' Tue Jun 30 20:03:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys645Arg' Q99728:p.Val695Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V L Wed May 02 16:56:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val695leu' Tue Jun 30 17:24:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val695Leu' 10 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2E, 2F, 2G, S2F. without DNA damage, PAR could not be detected, in vivo. IR, GLTN -gallotannin (GLTN), a cell-permeable PARG inhibitor that suppresses PAR degradation in vivo. PJ34- a PARP inhibitor After treatment with GLTN and subsequent covalent bond disruption, no PAR was detected (Fig S2F). brca1-par 1 10 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role ancillary ancillary Q99728:p.Cys645Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C R Wed May 02 18:32:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys645arg' Tue Jun 30 19:53:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys645Arg' Q99728:p.Val695Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V L Wed May 02 17:28:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val695leu' Tue Jun 30 17:55:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val695Leu' Q99728:p.Leu44Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L P Wed May 02 14:33:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu44pro' Tue Jun 30 17:33:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu44Pro' Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:13:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 19:56:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' 8 unspecified role unspecified role bait bait P38398:p.Cys61Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C G Wed May 02 19:06:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys61gly' Tue Jun 30 18:38:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys61Gly' association association 2Hi without DNA damage, PAR could not be detected, in vivo. IR PJ34 - a PARP inhibitor. bard1-par-4 3 7 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) colocalization colocalization 4B bard1-par-5 3 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 4Bi bard1-par-7 4 9 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction S2A par-bard1-5 6 7 unspecified role unspecified role prey prey Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:00:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 18:03:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99728:p.Cys645Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C R Wed May 02 19:31:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys645arg' Tue Jun 30 15:39:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys645Arg' Q99728:p.Val695Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V L Wed May 02 18:59:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val695leu' Tue Jun 30 18:23:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val695Leu' n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2B, S2A, S2C, S2D par-bard1-5 6 10 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction S2A par-bard1-6 5 7 unspecified role unspecified role prey prey Q99728:p.Cys645Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C R Wed May 02 18:48:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys645arg' Tue Jun 30 18:29:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys645Arg' Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:22:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 17:22:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' Q99728:p.Val695Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V L Wed May 02 19:56:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val695leu' Tue Jun 30 18:22:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val695Leu' 10 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2Ei, 2F, 2G. without DNA damage, PAR could not be detected, in vivo. IR, GLTN -gallotannin (GLTN), a cell-permeable PARG inhibitor that suppresses PAR degradation in vivo. PJ34 - a PARP inhibitor. par-brca1-1 5 7 unspecified role unspecified role ancillary ancillary Q99728:p.Leu44Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L P Wed May 02 17:10:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu44pro' Tue Jun 30 17:31:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu44Pro' Q99728:p.Val695Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V L Wed May 02 19:37:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val695leu' Tue Jun 30 16:00:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val695Leu' Q99728:p.Cys645Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C R Wed May 02 20:15:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys645arg' Tue Jun 30 17:01:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys645Arg' Q99728:p.Lys619Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:56:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys619ala' Tue Jun 30 18:58:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys619Ala' 10 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey P38398:p.Cys61Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C G Wed May 02 19:07:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys61gly' Tue Jun 30 15:57:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys61Gly' association association 2H without DNA damage, PAR could not be detected, in vivo. IR PJ34 - a PARP inhibitor. Very weak reaction with WT BRCA1.