IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer The cell line used in these experiments is not known and the authors have not supplied the missing info when requested. corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-vaco481 HUMAN VACO481- colon carcinoma with q546p mutation vaco481 VACO481 PMID: 10822375 useful anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase autoradiography autoradiography Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Gain of Interaction with IRS1 by p110a-Helical Domain Mutants Is Crucial for Their Oncogenic Functions. Cancer Cell (1535-6108) 2013 imex curation Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hao Y., Wang C., Cao B., Hirsch B.M., Song J., Markowitz S.D., Ewing R.M., Sedwick D., Liu L., Zheng W., Wang Z. Cancer - Interactions investigated in the context of cancer corrections done: I have not added a comment for Fig 3F as only mutant cell line was used. All IRS1 primers were found to be of Human origin by blasting, except the His/GST IRS1 M-C2 sequence, whose species of origin could not be determined.(-these sequences were not murine or rattine in origin). Primers for the Myc-3YF construct (Y608F, Y628F, Y658F) which judging by the amino acid numbering given would have been assumed to have been Murine in origin, were in fact determined to be Human on blasting. The authors have not replied to any requests for further info. wzheng@ujs.edu.cn,zxw22@case.edu Accepted 2013-AUG-05 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation pk3ca_human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Phosphoinositide-3-kinase catalytic alpha polypeptide Serine/threonine protein kinase PIK3CA PIK3CA Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha Phosphoinositide 3-kinase alpha protein protein human Homo sapiens Human MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN DIP protein P42336 original sequence version: 110 DIP protein P42336 original sequence version: 119 DIP protein P42336 original sequence version: 166 DIP protein P42336 original sequence version: 139 041487231A9A1207 p85a_human Phosphatidylinositol 3-kinase regulatory subunit alpha PIK3R1 GRB1 Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha protein protein human Homo sapiens Human MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGSSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR DIP protein P27986 original sequence version: 181 DIP protein P27986 original sequence version: 184 DIP protein P27986-1 original sequence version: 135 DIP protein P27986 original sequence version: 135 DIP protein P27986-1 original sequence version: 145 DIP protein P27986-1 original sequence version: 147 DIP protein P27986 original sequence version: 191 B9DAD8416C33140F irs1_human Insulin receptor substrate 1 IRS1 protein protein human Homo sapiens Human MASPPESDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKGHHDGAAALGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSNCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSARLHPPLNHSRSIPMPASRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGPSTLTAPNGHYILSRGGNGHRCTPGTGLGTSPALAGDEAASAADLDNRFRKRTHSAGTSPTITHQKTPSQSSVASIEEYTEMMPAYPPGGGSGGRLPGHRHSAFVPTRSYPEEGLEMHPLERRGGHHRPDSSTLHTDDGYMPMSPGVAPVPSGRKGSGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGGCSPDIGGGPSSSSSSSNAVPSGTSYGKLWTNGVGGHHSHVLPHPKPPVESSGGKLLPCTGDYMNMSPVGDSNTSSPSDCYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSTSSGRLLYAATADDSSSSTSSDSLGGGYCGARLEPSLPHPHHQVLQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRAREQQQQQQPLLHPPEPKSPGEYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ DIP protein P35568 original sequence version: 151 3C0EFD9E32B3E64A pi Phosphatidylinositol phosphatidylinositols PI PtdIns small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pik3ca-irs1 1 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:13:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 18:41:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' physical association physical association 1B A protein of ~170 kDa was detected using Flag tagged E545K knock-in (KI) cells, but not using Wild Type cells. pik3ca-irs1-2 4 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 19:50:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 17:04:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role prey prey association association 1Ci, supp 1C, 6Bi A protein of ~170 kDa was detected using Flag tagged E545K knock-in (KI) cells, but not using Wild Type cells. pik3ca-irs1-3 4 10 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role prey prey physical association physical association 1Cii, supp 1C. A protein of ~170 kDa was detected using Flag tagged E545K knock-in (KI) cells, but not using Wild Type cells. irs1-pik3ca 7 10 unspecified role unspecified role prey prey P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:38:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 19:07:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' 12 unspecified role unspecified role bait bait physical association physical association 1Di, supp1D. irs1-pik3ca-1 7 12 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:13:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 16:02:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1Dii, supp 1D irs1-pik3ca-3 9 12 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 19:28:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 17:10:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P42336:p.Glu542Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 18:46:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu542lys' Tue Jun 30 16:21:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu542Lys' P42336:p.Asn345Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain N K Wed May 02 14:54:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn345lys' Tue Jun 30 18:51:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn345Lys' P42336:p.Met1043Ile mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain M I Wed May 02 15:28:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met1043ile' Tue Jun 30 18:15:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met1043Ile' P42336:p.Gln546Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Q K Wed May 02 18:33:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln546lys' Tue Jun 30 19:19:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln546Lys' P42336:p.Glu545Gly mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E G Wed May 02 14:52:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545gly' Tue Jun 30 16:35:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Gly' P42336:p.Glu545Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:51:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545ala' Tue Jun 30 18:30:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Ala' P42336:p.Lys111Asn mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain K N Wed May 02 15:45:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys111asn' Tue Jun 30 17:26:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys111Asn' physical association physical association 1E, Supp1E. The presence of the following p110 mutations did not result in a statistically significant interaction with IRS1 :- H1047R, H1047L, G1049R, R88Q, C420R, P539R. The authors state that p110 molecules containing these mutations have "failed to interact with IRS1". from Fig 1E I would have entered these interactions as "decreasing" - but figure Supp1E does not support this. irs1-pik3ca-5 7 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 17:41:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 17:51:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' 12 unspecified role unspecified role bait bait association association 2A, supp 2A Insulin and pervanadate p110α E545K, but not the WT p110α, robustly associated with IRS1 under serum-starvation conditions in which tyrosine phosphorylation of IRS1 was undetectable. pik3ca-irs1-6 3 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 14:57:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 17:28:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association 2B, supp 2B. The p110α E545K-IRS1 interaction also did not require p85 because a truncated p110α E545K lacking the ABD domain required for p85 binding still associated with IRS1. pik3ca-irs1-7 4 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:10:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 17:28:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range abd_domain required to bind necessary binding region required to bind range range range range physical association physical association 2B, supp 2B.i The p110α E545K-IRS1 interaction also did not require p85 because a truncated p110α E545K lacking the ABD domain required for p85 binding still associated with IRS1. pik3ca-irs1-16 4 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:00:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 19:27:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' 12 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 2B, supp 2B.ii, The p110α E545K-IRS1 interaction also did not require p85 because a truncated p110α E545K lacking the ABD domain required for p85 binding still associated with IRS1. pik3ca-irs1-9 4 12 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range abd_domain required to bind necessary binding region required to bind range range range range association association 2B, supp 2B. iv The p110α E545K-IRS1 interaction also did not require p85 because a truncated p110α E545K lacking the ABD domain required for p85 binding still associated with IRS1. pik3ca-irs1-10 7 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:37:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 16:20:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' association association 2E, supp 2D Ablation of p85α and p85β enhanced rather than inhibited the interaction between p110α E545K and IRS1. pik3ca-irs1-11 2 10 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined glu545lys required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 12 unspecified role unspecified role prey prey purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction 2G, supp 2F recombinant IRS1 bound directly in vitro with p110α E545K but not WT p110α. irs1-pik3ca-6 5 10 unspecified role unspecified role prey prey purified purified P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 17:18:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Wed May 02 18:03:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 17:31:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma 12 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 3B,3D No binding was observed using the WT cells. irs1-pik3ca-7 5 12 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey purified purified P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 20:02:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 19:10:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma physical association physical association 3F irs1-pik3ca-9 9 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey purified purified P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:27:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 18:08:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3G/H, Supp 3D–S3G A deletion construct of IRS1 (IRS1Δ) devoid of both AA 607–623 and AA 936–962 regions greatly reduced its interaction with p110α E545K. Single region deletions could not interrupt the IRS1 mutant p110α interaction. The IRS1 deletion construct did not affect the IRS1-p85 interaction. I dont think the 2 deletions can be "required to bind" as there is still binding when they have been deleted...(so what is this showing?) pik3ca-irs1-13 4 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 19:27:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 18:15:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 inhibitor inhibitor unspecified role unspecified role P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 19:03:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 19:36:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain asp549,ser553 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain MOD:01067 -linker attached to peptide in Fmoc peptide synthesis association association 6Bii The stapled mutant peptide effectively disrupted IRS1-p110α E545K interaction.The stapled mutant peptide did not interfere with cellular p110α-p85 interaction. pik3ca-irs1-14 6 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P42336:p.Gln546Pro mutation mutation certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 15:02:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln546pro' Tue Jun 30 20:05:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln546Pro' 11 unspecified role unspecified role prey prey association association 6C pik3ca-irs1-15 6 10 inhibitor inhibitor unspecified role unspecified role P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 17:28:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 19:41:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' asp549,ser553 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain MOD:01067 -linker attached to peptide in Fmoc peptide synthesis region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P42336:p.Gln546Pro mutation mutation certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 19:09:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln546pro' Tue Jun 30 18:09:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln546Pro' 12 unspecified role unspecified role prey prey association association 6Ci The p110α E545K stapled peptide also disrupted protein interaction between p110α Q546P and IRS1 in Vaco481 CRC cells. irs1-pik3ca-10 4 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait purified purified n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey purified purified P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 17:40:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 16:55:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Supp 3H A deletion construct of IRS1 (IRS1Δ) devoid of both AA 607–623 and AA 936–962 regions greatly reduced its interaction with p110α E545K. The IRS1 deletion construct did not affect the IRS1-p85 interaction. Not sure how to annotate these deletions -I dont think the 2 deletions can be "required to bind" as there is still binding when they have been deleted... irs1-pik3ca-11 4 12 unspecified role unspecified role bait bait purified purified region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey purified purified P42336:p.Glu545Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 18:16:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 20:08:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' association association Supp 5D A deletion construct of IRS1 (IRS1Δ) devoid of both AA 607–623 and AA 936–962 regions greatly reduced its interaction with p110α. The deletions -I have entered as "required to bind" here as binding really is very much reduced.. pik3ca-irs1-17 3 12 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P42336:p.Glu545Lys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 14:37:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu545lys' Tue Jun 30 17:38:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu545Lys' region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range 10 inhibitor inhibitor unspecified role unspecified role asp549,ser553 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain association association supp 6D. the stapled mutant peptide also inhibited the interaction between IRS1 and ΔABD p110α E545K. pi-pik3ca-1 8 11 ancillary ancillary unspecified role unspecified role ni_sh2_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 10 enzyme enzyme unspecified role unspecified role purified purified glu545lys required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 13 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation Supp 6 J/K The stapled mutant peptide has no effect on the kinase activity of recombinant p110α, either alone or in the presence of IRS1, in vitro. Full-length IRS1 proteins also had no effect on the lipid kinase activity of p110α E545K, in vitro.