IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-a375p A375P Human Melanoma cell line a375p A375P Human Melanoma cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-sk_mel_23 Homo sapiens sk_mel_23 Human SK_MEL_23 - Melanoma cell line with WT p53 I cannot find a primary reference - as far back as 1991 they refer to it - but I dont think it would be possible to find any pmid reference before this... Houghton, A.N. et al. J. Exp. Med. 164 , 812-829 (1987). unpublished subline. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-wm278 WM278 Non-metastatic Human Melanoma cell line with mutation BRAF V600E wm278 WM278 Non-metastatic Melanoma cell line with mutation BRAF V600E Coriell Institute Catalog ID: WC00054 Wistar Collection anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-b16 Mus musculus melanoma tumor maintained in mouse. b16 mouse skin melanoma tumor maintained in C57/B1 mouse fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-wm115 Human WM115 Melanoma cell line wm115 WM115 Human melanoma cell line http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=CRL-1675&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-igr-37 Human IGR-37 Melanoma cell line from a lymph node metastasis igr-37 Human IGR-37 Melanoma cell line from a lymph node metastasis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. Cancer Cell (1535-6108) 2013 imex curation Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Not sure in Fig 1E if cell lines SK-MEL37 and IGR39 are meant to be positive or not - I am not convinced so have not entered (no reply from authors). Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Accepted 2013-JUL-08 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 ccnb_margl G2/mitotic-specific cyclin-B protein protein margl Marthasterias glacialis MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGMTKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFDNPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQETLFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQIRSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPSEIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKKYSSAKFMNVSTISALTSSTVMDLADQMC 3DAAEECA7FBDA917 iaspp_human RelA-associated inhibitor PPP1R13L IASPP NKIP1 PPP1R13BL RAI Inhibitor of ASPP protein NFkB-interacting protein 1 PPP1R13B-like protein protein protein human Homo sapiens Human MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQPQPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV DIP protein Q8WUF5 original sequence version: 129 DIP protein Q8WUF5 original sequence version: 52 7BEEF239B7CA9C70 cdk1_margl protein protein margl Marthasterias glacialis iaspp_mouse RelA-associated inhibitor Ppp1r13l Nkip1 Inhibitor of ASPP protein NFkB-interacting protein 1 PPP1R13B-like protein protein protein mouse Mus musculus Mouse MDSEAFQHARDLLDLNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGAQAGVPSRMARYSTSPVPEHFGSRGSPQKIATDGIEARFGRSESAPSLHPYSPLSPKGRPSSPRTPIYLQPDTYSSLDRAPSPRPRAFDGAGSPHGRAPSPRPGIGPVRQPGPSTPFDYLGRAGSPRGSPLAEGPQAFFPERGPSPRPPAAAYDTAGTFGSPLLGAGGSAFTPPLRAQDDSTLRRRPPKAWNESDLDVAYEKKSSQTASYERLDVFTRPASPGLQLLPWRESSLDGLGASGKDHLTSATLPRNYKVSPLASDRRSDVGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRSTPRQRPIPLSMIFKLQNAFWEHGAGRAVLPGSPIFSRAPPPKLPPQPPPQPQMQPQPQPQPQMQPQSQAQPQTPAPQQTWSPMNEGLLKSPAELEPEPELEVLLAPVEEAGDADEGTVTRPLSPTRLQPALPPEAQTVPELEEVARVLAEIPRPLKRRGSMEQSPAVALPPTHKKQYQQIINRLFHRHGGPGPGGPEPELSTITEGSEARAGPPAPAPPAPIPPPAPPQSSPPEQPQSMEMRSVLRKVGSPRKARRARLNPLVLLLDAALTGELDVVQQAVKEMNDPSQPNEEGITALHNAICGANYPIVDFLIAAGANVNSPDSHGWTPLHCAASCNDTAICTALVQHGAAIFATTLSDGATAIEKCDPYREGYADCATYLADVEQSMGLMHNGVVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWASLHGQEGYVPRNYFGLFPRVKSQRSKI 57AC88589BC8AA8B cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM DIP protein P06493 original sequence version: 185 DIP protein P06493 original sequence version: 162 DIP protein P06493 original sequence version: 184 DIP protein P06493 original sequence version: 97 DIP protein P06493 original sequence version: 192 942D79448EFE490A tp53-ppp1r13l 8 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association 1E p53 selectively coimmunoprecipitated slow-migrating (nuclear) iASPP in the panel of melanoma cell lines tested. tp53-ppp1r13l-1 5 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association 1E p53 selectively coimmunoprecipitated slow-migrating (nuclear) iASPP in the panel of melanoma cell lines tested. tp53-ppp1r13l-2 6 12 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association 1E, 7D. p53 selectively coimmunoprecipitated slow-migrating iASPP in the panel of melanoma cell lines tested. (CIP) -calf intestine alkaline phosphatase. JNJ-7706621 (JNJ), a CDK1 inhibitor. Nocodazole (an anti-neoplastic agent interfering with microtubule polymerization.) It increases amounts of the slow-migrating form of iASPP. tp53-ppp1r13l-3 9 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association 1E p53 selectively coimmunoprecipitated slow-migrating (nuclear) iASPP in the panel of melanoma cell lines tested. ppp1r13l-cdk1-4 4 13 unspecified role unspecified role ancillary ancillary 15 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 3B ccnb1-cdk1 10 13 ancillary ancillary unspecified role unspecified role 14 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8WUF5:p.[Ser84Ala;Ser113Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:12:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84ala,ser113ala' Tue Jun 30 16:42:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser84Ala;Ser113Ala]' Q8WUF5:p.Ser84Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:23:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84ala' Tue Jun 30 20:24:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser84Ala' Q8WUF5:p.Ser113Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:19:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser113ala' Tue Jun 30 17:38:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser113Ala' ecoli Escherichia coli (strain K12) 15 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation 3C/D ppp1r13l-cdk1-1 4 14 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8WUF5:p.Ser84Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:58:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84ala' Tue Jun 30 18:47:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser84Ala' Q8WUF5:p.Ser113Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:24:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser113ala' Tue Jun 30 18:19:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser113Ala' Q8WUF5:p.Ser113Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S D Wed May 02 19:57:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser113asp' Tue Jun 30 20:09:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser113Asp' Q8WUF5:p.[Ser84Asp;Ser113Asp] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:07:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84asp,ser113asp' Tue Jun 30 15:38:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser84Asp;Ser113Asp]' Q8WUF5:p.Ser84Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:02:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84asp' Tue Jun 30 18:03:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser84Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction 4A/B ppp1r13l-cdk1-2 4 14 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. physical association physical association 4C ppp1r13l-cdk1-3 4 14 unspecified role unspecified role prey prey human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. 14 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 4D ppp1r13l-ppp1r13l 4 14 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8WUF5:p.Thr722Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 16:25:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr722ala' Tue Jun 30 20:09:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr722Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WUF5:p.Tyr814Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:08:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr814ala' Tue Jun 30 18:26:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr814Ala' Q8WUF5:p.[Leu724Ala;Tyr814Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 18:36:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu724ala,tyr814ala' Tue Jun 30 18:02:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu724Ala;Tyr814Ala]' Q8WUF5:p.Leu724Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:45:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu724ala' Tue Jun 30 17:34:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu724Ala' Q8WUF5:p.[Thr722Ala;Asn813Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain T A Fri Jul 22 10:53:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asn813ala,thr722ala' Wed May 02 15:15:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr722ala,asn813ala' Tue Jun 30 16:23:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr722Ala;Asn813Ala]' Q8WUF5:p.Asn813Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 14:51:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn813ala' Tue Jun 30 18:30:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn813Ala' Q8WUF5:p.[Thr722Ala;Tyr814Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:14:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr722ala,tyr814ala' Tue Jun 30 18:56:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr722Ala;Tyr814Ala]' Q8WUF5:p.[Leu724Ala;Asn813Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain N A Wed May 02 16:31:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu724ala,asn813ala' Tue Jun 30 17:07:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu724Ala;Asn813Ala]' ecoli Escherichia coli (strain K12) 71 74 direct interaction direct interaction 4F, Supp 4B. ppp1r13l-ppp1r13l-1 4 14 unspecified role unspecified role prey prey Q8WUF5:p.[Ser84Asp;Ser113Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 14:44:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84asp,ser113asp' Tue Jun 30 16:01:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser84Asp;Ser113Asp]' region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 5A tp53-ppp1r13l-4 1 12 unspecified role unspecified role bait bait purified purified 14 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WUF5:p.[Ser84Asp;Ser113Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:25:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84asp,ser113asp' Tue Jun 30 19:16:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser84Asp;Ser113Asp]' physical association physical association 5B cdk1-ppp1r13l-1 3 14 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component purified purified colocalization colocalization 7B ppp1r13l-tp53-3 7 11 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component colocalization colocalization 7K JNJ-7706621 (JNJ), a CDK1 inhibitor. cdk1-ppp1r13l-2 2 12 unspecified role unspecified role neutral component neutral component purified purified 14 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp 8I JNJ-7706621 (JNJ), a CDK1 inhibitor.