IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Relief of Profound Feedback Inhibition of Mitogenic Signaling by RAF Inhibitors Attenuates Their Activity in BRAFV600E Melanomas. Relief of Profound Feedback Inhibition of Mitogenic Signaling by RAF Inhibitors Attenuates Their Activity in BRAFV600E Melanomas. Cancer Cell (1535-6108) 2012 imex curation Lito P., Pratilas C.A., Joseph E.W., Tadi M., Halilovic E., Zubrowski M., Huang A., Wong W.L., Callahan M.K., Merghoub T., Wolchok J.D., de Stanchina E., Chandarlapaty S., Poulikakos P.I., Fagin J.A., Rosen N. Cancer - Interactions investigated in the context of cancer This cell line Malme-3M has the BRAF(V600E) mutation. RBD pull-down assays have not been included due to lack of antibody target specificity and/or origin of GST-Raf1. rosenn@mskcc.org 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions human-malme-3m MALME-3M Melanoma cell line malme-3m Human Malme-3M - Melanoma cell line Contains BRAF .V600E mutation. Derived from lung metastic tissue. https://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/AllCollectionSearch/tabid/1069/Default.aspx anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lito P., Pratilas C.A., Joseph E.W., Tadi M., Halilovic E., Zubrowski M., Huang A., Wong W.L., Callahan M.K., Merghoub T., Wolchok J.D., de Stanchina E., Chandarlapaty S., Poulikakos P.I., Fagin J.A., Rosen N. Cancer - Interactions investigated in the context of cancer This cell line Malme-3M has the BRAF(V600E) mutation. RBD pull-down assays have not been included due to lack of antibody target specificity and/or origin of GST-Raf1. rosenn@mskcc.org Accepted 2012-NOV-29 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Relief of Profound Feedback Inhibition of Mitogenic Signaling by RAF Inhibitors Attenuates Their Activity in BRAFV600E Melanomas. Relief of Profound Feedback Inhibition of Mitogenic Signaling by RAF Inhibitors Attenuates Their Activity in BRAFV600E Melanomas. Cancer Cell (1535-6108) 2012 imex curation Lito P., Pratilas C.A., Joseph E.W., Tadi M., Halilovic E., Zubrowski M., Huang A., Wong W.L., Callahan M.K., Merghoub T., Wolchok J.D., de Stanchina E., Chandarlapaty S., Poulikakos P.I., Fagin J.A., Rosen N. Cancer - Interactions investigated in the context of cancer This cell line Malme-3M has the BRAF(V600E) mutation. RBD pull-down assays have not been included due to lack of antibody target specificity and/or origin of GST-Raf1. rosenn@mskcc.org 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lito P., Pratilas C.A., Joseph E.W., Tadi M., Halilovic E., Zubrowski M., Huang A., Wong W.L., Callahan M.K., Merghoub T., Wolchok J.D., de Stanchina E., Chandarlapaty S., Poulikakos P.I., Fagin J.A., Rosen N. Cancer - Interactions investigated in the context of cancer This cell line Malme-3M has the BRAF(V600E) mutation. RBD pull-down assays have not been included due to lack of antibody target specificity and/or origin of GST-Raf1. rosenn@mskcc.org Accepted 2012-NOV-29 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation traf3_human TNF receptor-associated factor 3 TRAF3 CAP1 CRAF1 CD40 receptor-associated factor 1 CD40-binding protein LMP1-associated protein 1 CAP-1 RING-type E3 ubiquitin transferase TRAF3 protein protein human Homo sapiens Human MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP DIP protein Q13114 original sequence version: 158 DIP protein Q13114 original sequence version: 127 DIP protein Q13114 original sequence version: 146 DIP protein Q13114 original sequence version: 151 9456E440C0A90FBF braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH DIP protein P15056 original sequence version: 182 DIP protein P15056 original sequence version: 133 DIP protein P15056 original sequence version: 147 DIP protein P15056 original sequence version: 173 DIP protein P15056 original sequence version: 178 DIP protein P15056 original sequence version: 188 DIP protein P15056 original sequence version: 193 DIP protein P15056 original sequence version: 195 DIP protein P15056 original sequence version: 146 DIP protein P15056 original sequence version: 153 0798C2AAB487E813 braf-traf3 1 4 unspecified role unspecified role bait bait P15056:p.Val600Glu mutation mutation certain certain sequence position certain certain certain sequence position certain V E Wed May 02 15:53:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val600glu' Tue Jun 30 19:41:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val600Glu' 3 unspecified role unspecified role prey prey physical association physical association 2D, 6D PD0325901 - A MEK inhibitor Neratinib -an irreversible inhibitor of EGFR/HER kinases. braf-traf3-1 2 4 unspecified role unspecified role bait bait P15056:p.Val600Glu mutation mutation certain certain sequence position certain certain certain sequence position certain V E Wed May 02 19:50:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val600glu' Tue Jun 30 17:41:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val600Glu' 3 unspecified role unspecified role prey prey physical association physical association 6D PD0325901 - MEK inhibitor Neratinib -an irreversible inhibitor of EGFR/HER kinases.