IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunostaining immunostaining Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-hcc-1954 HCC-1954 Ductal Carcinoma cell line hcc-1954 HCC-1954 Human Breast (Ductal) Carcinoma cell line ATCC Number = CRL-2338 HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. ER-, PR-, ERBB2+. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer The image legend refers to this cell line as HCC-1953, but this cell line is not referred to again in this paper, or elsewhere. Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-hacat Homo sapiens immortalised keratinocytes hacat human immortalised keratinocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI The anti-ErbB2 antibody utilized for precipitation recognizes both wildtype (Human) ErbB2 and mNeuNT, but as Hek293 cells express little endogenous Erbb2 there is not a detection problem. PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-bt474 Homo sapiens breast ductal carcinoma cell line bt474 Human breast ductal carcinoma cell line BT474 Human breast ductal carcinoma cell line established from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. PLoS ONE 2012 imex curation Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Only protein-protein interactions human-mda_mb_468 Homo sapiens MDA-MB-468 mda_mb_468 MDA-MB-468 breast adenocarcinoma Species: human, Black; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Fig 2B (co-loc) not entered as EphB1-Fc is used to bind to cell surface EphB1 ligands, and the anti-IgG used to detect the resulting complex may not be EphB1 specific. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org Accepted 2012-FEB-10 by JYOTI PLoS ONE 2012 Only protein-protein interactions imex curation erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV DIP protein P04626 original sequence version: 198 DIP protein P04626 original sequence version: 122 DIP protein P04626 original sequence version: 206 DIP protein P04626 original sequence version: 195 DIP protein P04626 original sequence version: 150 DIP protein P04626 original sequence version: 179 DIP protein P04626 original sequence version: 146 DIP protein P04626 original sequence version: 147 39E9DFDA04DCF962 efnb1_human Ephrin-B1 EPH-related receptor tyrosine kinase ligand 2 EFL3 EFNB1 EFL-3 EPLG2 LERK2 ELK ligand protein protein human Homo sapiens Human MARPGQRWLGKWLVAMVVWALCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDGFFNSKVALFAAVGAGCVIFLLIIIFLTVLLLKLRKRHRKHTQQRAAALSLSTLASPKGGSGTAGTEPSDIIIPLRTTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV 473DD2F1A5BF89DE erbb2_mouse Receptor tyrosine-protein kinase erbB-2 Erbb2 Kiaa3023 Neu Proto-oncogene Neu Proto-oncogene c-ErbB-2 p185erbB2 protein protein mouse Mus musculus Mouse MELAAWCRWGFLLALLSPGAAGTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPANASLSFLQDIQEVQGYMLIAHNRVKHVPLQRLRIVRGTQLFEDKYALAVLDNRDPLDNVTTAAPGRTPEGLRELQLRSLTEILKGGVLIRGNPQLCYQDMVLWKDVLRKNNQLAPVDMDTNRSRACPPCAPTCKDNHCWGESPEDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALITYNTDTFESMLNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEVTAEDGTQRCEKCSKPCAGVCYGLGMEHLRGARAITSDNIQEFAGCKKIFGSLAFLPESFDGNPSSGVAPLKPEHLQVFETLEEITGYLYISAWPESFQDLSVFQNLRVIRGRILHDGAYSLTLQGLGIHSLGLRSLRELGSGLALIHRNTHLCFVNTVPWDQLFRNPHQALLHSGNRPEEACGLEGLVCNSLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVWKGLPREYVRGKHCLPCHPECQPQNSSETCYGSEADQCEACAHYKDSSSCVARCPSGVKPDLSYMPIWKYPDEEGICQPCPINCTHSCVDLDERGCPAEQRASPVTFIIATVVGVLLFLIIVVVIGILIKRRRQKIRKYTMRRLLQETELVEPLTPSGAVPNQAQMRILKETELRKLKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVREHRGRLGSQDLLNWCVQIAKGMSYLEEVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPSSPMDSTFYRSLLEDDDMGELVDAEEYLVPQQGFFSPDPALGTGSTAHRRHRSSSARSGGGELTLGLEPSEEEPPRSPLAPSEGAGSDVFDGDLAVGVTKGLQSLSPHDLSPLQRYSEDPTLPLPPETDGYVAPLACSPQPEYVNQPEVRPQSPLTPEGPPPPIRPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLAPRAGTASQPHPSPAFSPAFDNLYYWDQNSSEQGPPPSTFEGTPTAENPEYLGLDVPV DIP protein P70424 original sequence version: 159 6040978428B93A28 src_human Proto-oncogene tyrosine-protein kinase Src pp60c-src SRC SRC1 Proto-oncogene c-Src protein protein human Homo sapiens Human MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL DIP protein P12931 original sequence version: 188 DIP protein P12931 original sequence version: 189 DIP protein P12931 original sequence version: 190 DIP protein P12931 original sequence version: 191 DIP protein P12931 original sequence version: 115 DIP protein P12931 original sequence version: 116 DIP protein P12931 original sequence version: 164 DIP protein P12931 original sequence version: 142 DIP protein P12931 original sequence version: 143 DIP protein P12931 original sequence version: 150 DIP protein P12931 original sequence version: 160 DIP protein P12931 original sequence version: 184 DIP protein P12931 original sequence version: 155 DIP protein P12931-1 original sequence version: 155 DIP protein P12931 original sequence version: 202 C1908084683E5DE8 erbb2-efnb1 9 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 1E EphrinB1 associated with ErbB2 in lysates from shPTPN13 cells. erbb2-efnb1-1 3 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 2A knock-down of PTPN13 in HaCaT cells (shPTPN13) enhanced pull-down of EphrinB1 with ErbB2. erbb2-efnb1-2 7 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 2A, 2C. erbb2-efnb1-3 6 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 2A, 2C. erbb2-efnb1-4 2 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 2A erbb2-efnb1-5 8 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 2C EphrinB1 associated with ErbB2 in lysates from shPTPN13 cells. erbb2-efnb1-6 5 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 2E EphrinB1 associated with transfected ErbB2. erbb2-efnb1-7 5 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait P70424:p.Val660Glu mutation mutation certain certain sequence position certain certain certain sequence position certain V E Wed May 02 17:01:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val660glu' Tue Jun 30 19:27:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val660Glu' physical association physical association 2E Expression of mNeuNT with EphrinB1 increases the amount of EphrinB1 precipitated, and it's phosphorylation. phosphatase null mutant PTPN13C/S efnb1-erbb2 1 11 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component P70424:p.Val660Glu mutation mutation certain certain sequence position certain certain certain sequence position certain V E Wed May 02 16:00:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val660glu' Tue Jun 30 15:38:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val660Glu' colocalization colocalization 2F Only expression of mNeuNT results in phosphorylated EphrinB present at the cell surface. erbb2-efnb1-8 5 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait P70424:p.Val660Glu mutation mutation certain certain sequence position certain certain certain sequence position certain V E Wed May 02 15:30:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val660glu' Tue Jun 30 18:21:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val660Glu' physical association physical association 3A, 3B. PP2, a potent Src inhibitor. efnb1-erbb2-1 4 10 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait physical association physical association 4B All constructs localized to the membrane. erbb2-efnb1-9 4 10 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4C Erbb2 associated with all Ephrin mutants. efnb1-erbb2-3 1 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 4D Endogenous EphrinB1 associated with all ErbB2 mutants. efnb1-erbb2-4 1 10 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 4D All constructs localised to the membrane.