IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-md11 MD11-Human glioblastoma multiforme cell line md11 MD11-Human glioblastoma multiforme cell line Primary neurosphere-derived cells isolated from fresh surgical specimens of GBM and having an enriched GIC (GlioBlastoma-Initiating Cells) population. confocal microscopy confocal microscopy immunostaining immunostaining Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer Fig S4Q not entered as apperas to gene activity not any protein interaction. e.g. "The TOPdGFP reporter construct contains four consensus Lef binding sites and a minimal cFos promoter, driving a destabilized GFP transgene. This reporter should only be transcriptionally active in the presence of both stabilized beta-catenin and Lef/Tcf proteins. " (Human) M11 cells were injected into mouse brain, and the resulting tumours were stained. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. Figure 1G:-Cells were treated with 20 ng/ml Wnt3a, and cytoplasmic or nuclear extracts were prepared at various time points. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. Figure 1G:-Cells were treated with 20 ng/ml Wnt3a, and cytoplasmic or nuclear extracts were prepared at various time points. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-hs1783 HUMAN HS1783 - Glioma cell line hs1783 Human HS1783 http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=HTB-13&Template=cellBiology anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-md11 MD11-Human glioblastoma multiforme cell line md11 MD11-Human glioblastoma multiforme cell line Primary neurosphere-derived cells isolated from fresh surgical specimens of GBM and having an enriched GIC (GlioBlastoma-Initiating Cells) population. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. no Figure 1G:-Cells were treated with 20 ng/ml Wnt3a, and cytoplasmic or nuclear extracts were prepared at various time points. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. Fig S3C and D could have been combined in ONE interaction rather than 2 - with a much longer comment, but for sake of clarity I have left as two interactions. ( no mutation entered for S3D.) The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer I have put an InterPro X Ref on the Feature (Arm rpts) - is this at the right level? The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-md20 Human MD20-Human glioblastoma multiforme cell line md20 MD20-Human glioblastoma multiforme cell line Primary neurosphere-derived cells isolated from fresh surgical specimens of GBM and having an enriched GIC (GlioBlastoma-Initiating Cells) population. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-glioblastoma Homo sapiens glioblastoma glioblastoma Glioblastoma Glioblastoma is the same as 'Glioblastoma multiforme' (GBM) -is the same as Astrocytoma Grade 4. confocal microscopy confocal microscopy immunostaining immunostaining Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunostaining immunostaining Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-md11 MD11-Human glioblastoma multiforme cell line md11 MD11-Human glioblastoma multiforme cell line Primary neurosphere-derived cells isolated from fresh surgical specimens of GBM and having an enriched GIC (GlioBlastoma-Initiating Cells) population. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-md11 MD11-Human glioblastoma multiforme cell line md11 MD11-Human glioblastoma multiforme cell line Primary neurosphere-derived cells isolated from fresh surgical specimens of GBM and having an enriched GIC (GlioBlastoma-Initiating Cells) population. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. RE-chIP experiment The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions mouse-glioma Mouse-Glioma glioma Glioma confocal microscopy confocal microscopy immunostaining immunostaining Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. FoxM1 Promotes β-Catenin Nuclear Localization and Controls Wnt Target-Gene Expression and Glioma Tumorigenesis. Cancer Cell (1535-6108) 2011 imex curation Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-hs683 Human HS683 - Glioma cell line hs683 Human HS683 - Glioma cell line http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=HTB-138&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang N., Wei P., Gong A., Chiu W.T., Lee H.T., Colman H., Huang H., Xue J., Liu M., Wang Y., Sawaya R., Xie K., Yung W.K., Medema R.H., He X., Huang S. Cancer - Interactions investigated in the context of cancer The molecule "TCF4" is the protein "Transcription factor 7-like 2" (Q9NQB0 (TF7L2_HUMAN)) whose Gene ID has a synonym of TCF4. The authors confirm that all constructs used in this publication were Human in origin. They also confirm that TCF4 (TCF7L2) corresponds to UniProt entry Q9NQB0. suhuang@mdanderson.org Accepted 2012-JAN-24 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation tf7l2_human Transcription factor 7-like 2 TCF7L2 TCF4 HMG box transcription factor 4 T-cell-specific transcription factor 4 protein protein human Homo sapiens Human MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTLHFQSGSTHYSAYKTIEHQIAVQYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWLVPQQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKRDKQPGETNEHSECFLNPCLSLPPITDLSAPKKCRARFGLDQQNNWCGPCRRKKKCVRYIQGEGSCLSPPSSDGSLLDSPPPSPNLLGSPPRDAKSQTEQTQPLSLSLKPDPLAHLSMMPPPPALLLAEATHKASALCPNGALDLPPAALQPAAPSSSIAQPSTSSLHSHSSLAGTQPQPLSLVTKSLE DIP protein Q9NQB0 original sequence version: 101 DIP protein Q9NQB0 original sequence version: 99 DIP protein Q9NQB0 original sequence version: 116 4DD2D3CC814AE16E q08050-1 Forkhead box protein M1 FOXM1 FKHL16 HFH11 MPP2 WIN Forkhead-related protein FKHL16 Hepatocyte nuclear factor 3 forkhead homolog 11 M-phase phosphoprotein 2 MPM-2 reactive phosphoprotein 2 Transcription factor Trident Winged-helix factor from INS-1 cells FOXM1-c FoxM1C protein protein human Homo sapiens Human MKTSPRRPLILKRRRLPLPVQNAPSETSEEEPKRSPAQQESNQAEASKEVAESNSCKFPAGIKIINHPTMPNTQVVAIPNNANIHSIITALTAKGKESGSSGPNKFILISCGGAPTQPPGLRPQTQTSYDAKRTEVTLETLGPKPAARDVNLPRPPGALCEQKRETCADGEAAGCTINNSLSNIQWLRKMSSDGLGSRSIKQEMEEKENCHLEQRQVKVEEPSRPSASWQNSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWKNSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRYLTLDQVFKPLDPGSPQLPEHLESQQKRPNPELRRNMTIKTELPLGARRKMKPLLPRVSSYLVPIQFPVNQSLVLQPSVKVPLPLAASLMSSELARHSKRVRIAPKVLLAEEGIAPLSSAGPGKEEKLLFGEGFSPLLPVQTIKEEEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTSQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ 963CAC8FE7498E9B q08050-2 Forkhead box protein M1 FOXM1 FKHL16 HFH11 MPP2 WIN Forkhead-related protein FKHL16 Hepatocyte nuclear factor 3 forkhead homolog 11 M-phase phosphoprotein 2 MPM-2 reactive phosphoprotein 2 Transcription factor Trident Winged-helix factor from INS-1 cells FOXM1-b FoxM1B protein protein human Homo sapiens Human MKTSPRRPLILKRRRLPLPVQNAPSETSEEEPKRSPAQQESNQAEASKEVAESNSCKFPAGIKIINHPTMPNTQVVAIPNNANIHSIITALTAKGKESGSSGPNKFILISCGGAPTQPPGLRPQTQTSYDAKRTEVTLETLGPKPAARDVNLPRPPGALCEQKRETCADGEAAGCTINNSLSNIQWLRKMSSDGLGSRSIKQEMEEKENCHLEQRQVKVEEPSRPSASWQNSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWKNSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRYLTLDQVFKQQKRPNPELRRNMTIKTELPLGARRKMKPLLPRVSSYLVPIQFPVNQSLVLQPSVKVPLPLAASLMSSELARHSKRVRIAPKVLLAEEGIAPLSSAGPGKEEKLLFGEGFSPLLPVQTIKEEEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTSQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ 3224A22B1434A531 axin2_human_gene gene gene human Homo sapiens Human foxm1_mouse Forkhead box protein M1 Forkhead homolog 16 Winged-helix transcription factor Trident Foxm1 Fkh16 protein protein mouse Mus musculus Mouse MRTSPRRPLILKRRRLPLPVQNAPSETSEEEAKRSPAQPEPAPAQASQEVAESSSCKFPAGIKIINHPTTPNTQVVAIPSNADIQSIITALTAKGKESGTSGPNRFILISSGGPSSHPSQPQAHSSRDSKRAEVITETLGPKPAAKGVPVPKPPGAPPRQRQESYAGGEAAGCTLDNSLTNIQWLGKMSSDGLGPCSVKQELEEKENCHLEQNRVKVEEPSGVSTSWQDSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWKNSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRHLTLDQVFKPLEPGSPQSPEHLESQQKRPNPELHRNVTIKTEIPLGARRKMKPLLPRVSSYLEPIQFPVNQSLVLQPSVKVPFRLAASLMSSELARHSKRVRIAPKVLLSSEGIAPLPATEPPKEEKPLLGGEGLLPLLPIQSIKEEEMQPEEDIAHLERPIKVESPPLEEWPSPCASLKEELSNSWEDSSCSPTPKPKKSYCGLKSPTRCVSEMLVTKRREKREVSRSRRKQHLQPPCLDEPDLFFPEDSSTFRPAVELLAESSEPAPHLSCPQEEGGPFKTPIKETLPVSSTPSKSVLSRDPESWRLTPPAKVGGLDFSPVRTPQGAFGLLPDSLGLMELNTTPLKSGPLFDSPRELLNSEPFDLASDPFGSPPPPHVEGPKPGSPELQIPSLSANRSLTEGLVLDTMNDSLSKILLDISFPGLEEDPLGPDNINWSQFIPELR AA614CB940EE7492 foxm1_human Forkhead box protein M1 Hepatocyte nuclear factor 3 forkhead homolog 11 Winged-helix factor from INS-1 cells M-phase phosphoprotein 2 MPM-2 reactive phosphoprotein 2 Transcription factor Trident Forkhead-related protein FKHL16 WIN FOXM1 FKHL16 HFH11 MPP2 protein protein human Homo sapiens Human MKTSPRRPLILKRRRLPLPVQNAPSETSEEEPKRSPAQQESNQAEASKEVAESNSCKFPAGIKIINHPTMPNTQVVAIPNNANIHSIITALTAKGKESGSSGPNKFILISCGGAPTQPPGLRPQTQTSYDAKRTEVTLETLGPKPAARDVNLPRPPGALCEQKRETCADGEAAGCTINNSLSNIQWLRKMSSDGLGSRSIKQEMEEKENCHLEQRQVKVEEPSRPSASWQNSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWKNSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRYLTLDQVFKPLDPGSPQLPEHLESQQKRPNPELRRNMTIKTELPLGARRKMKPLLPRVSSYLVPIQFPVNQSLVLQPSVKVPLPLAASLMSSELARHSKRVRIAPKVLLAEEGIAPLSSAGPGKEEKLLFGEGFSPLLPVQTIKEEEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTSQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ DIP protein Q08050 original sequence version: 142 963CAC8FE7498E9B ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E myc_human_gene Human c-myc gene gene gene human Homo sapiens Human ctnb1_mouse Catenin beta-1 Beta-catenin Ctnnb1 Catnb protein protein mouse Mus musculus Mouse MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein Q02248 original sequence version: 128 DIP protein Q02248 original sequence version: 135 DIP protein Q02248 original sequence version: 147 DIP protein Q02248 original sequence version: 187 DIP protein Q02248 original sequence version: 140 D708F170A3FBED6E lef1_human_gene HUMAN LEF1 gene gene human Homo sapiens Human foxm1-ctnnb1 6 26 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 1A, Supp. Figs. 1A, 1B ctnnb1-foxm1 10 25 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 26 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined arm_rpts_11+12 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 1B (LHS), 4A. foxm1-ctnnb1-1 12 26 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 25 unspecified role unspecified role bait bait forkhead_box_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 1B (RHS), 4B. ctnnb1-foxm1-3 16 25 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig 2A, S2A. Wnt3a increased the levels and nuclear translocation of both beta catenin and FoxM1. foxm1-ctnnb1-3 7 25 unspecified role unspecified role bait bait 26 unspecified role unspecified role prey prey physical association physical association Fig 1E ctnnb1-foxm1-1 7 26 unspecified role unspecified role bait bait 25 unspecified role unspecified role prey prey physical association physical association Fig 1E ctnnb1-foxm1-2 11 26 unspecified role unspecified role bait bait 25 unspecified role unspecified role prey prey physical association physical association Fig 1E foxm1-ctnnb1-4 11 25 unspecified role unspecified role bait bait 26 unspecified role unspecified role prey prey physical association physical association Fig 1E foxm1-ctnnb1-8 19 20 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 1F foxm1-ctnnb1-7 19 20 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait physical association physical association Fig 1F In the absence of T7 tagged FoxM1, only a weak interaction with beta catenin and TCF4/TCF7L2 was observed. foxm1-ctnnb1-9 5 25 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey physical association physical association Fig 1F foxm1-ctnnb1-10 3 26 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined forkhead_box_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1G, S1C, 4C. Wnt3a promoted nuclear translocation of both FoxM1 and beta-catenin w.r.t. time, with a decrease in cytoplasmic beta-catenin levels. ctnnb1-foxm1-4 14 26 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined forkhead_box_and_nls_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig 2A Wnt3a increased the levels and nuclear translocation of both beta catenin and FoxM1. foxm1-lef1 15 29 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait association association Fig 6B, 6G, 6H. FoxM1, beta-catenin, and TCF4 bound to the WRE region of the LEF-1 promoter in MD11 cells. tcf7l2-lef1 15 20 unspecified role unspecified role bait bait 29 unspecified role unspecified role prey prey association association Fig 6B FoxM1, beta-catenin, and TCF4 bound to the WRE region of the LEF-1 promoter in MD11 cells. ctnnb1-lef1-6 15 26 unspecified role unspecified role bait bait 29 unspecified role unspecified role prey prey association association Fig 6B,6G,6H. FoxM1, beta-catenin, and TCF4 bound to the WRE region of the LEF-1 promoter in MD11 cells. tcf7l2-lef1-1 9 20 unspecified role unspecified role bait bait 29 unspecified role unspecified role prey prey association association Fig 6C Wnt3a stimulation caused a dramatic increase in FoxM1 and beta-catenin binding to the WRE region of the LEF-1 promoter, which was occupied by TCF4/TF7L2 constitutively in 293T cells. foxm1-lef1-1 9 29 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait association association Fig 6C Wnt3a stimulation caused a dramatic increase in FoxM1 and beta-catenin binding to the WRE region of the LEF-1 promoter, which was occupied by TCF4/TF7L2 constitutively in 293T cells. ctnnb1-lef1-7 9 26 unspecified role unspecified role bait bait 29 unspecified role unspecified role prey prey association association Fig 6C Wnt3a stimulation caused a dramatic increase in FoxM1 and beta-catenin binding to the WRE region of the LEF-1 promoter, which was occupied by TCF4/TF7L2 constitutively in 293T cells. tcf7l2-axin2 15 23 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait association association Fig 6D FoxM1, beta-catenin, and TCF4 bound to the WRE region of the Axin2 promoter in MD11 cells. ctnnb1-axin2 15 26 unspecified role unspecified role bait bait 23 unspecified role unspecified role prey prey association association Fig 6D FoxM1, beta-catenin, and TCF4 bound to the WRE region of the Axin2 promoter in MD11 cells. foxm1-axin2 15 25 unspecified role unspecified role bait bait 23 unspecified role unspecified role prey prey association association Fig 6D FoxM1, beta-catenin, and TCF4 bound to the WRE region of the Axin2 promoter in MD11 cells. foxm1-ctnnb1-11 17 26 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey promoter_wnt_response-element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role bait bait association association Fig 6F FoxM1 associated with the WREs in the LEF-1 promoter that had been immunoprecipitated by the anti-β-catenin or anti-TCF4 antibody. foxm1-lef1-2 17 20 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait 29 unspecified role unspecified role prey prey promoter_wnt_response-element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 6F FoxM1 associated with the WREs in the LEF-1 promoter that had been immunoprecipitated by the anti-β-catenin or anti-TCF4 antibody. tcf7l2-ctnnb1-10 1 20 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey association association 6I binding between β-catenin and TCF4 was decreased upon FoxM1 depletion. tcf7l2-foxm1-3 4 28 unspecified role unspecified role ancillary ancillary 25 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 6K Depletion of beta-catenin by a siRNA or genetic deletion reduced or abolished the association between FoxM1 and TCF4. foxm1-ctnnb1-12 1 22 unspecified role unspecified role bait bait 26 unspecified role unspecified role prey prey physical association physical association S1K foxm1-ctnnb1-14 1 26 unspecified role unspecified role prey prey 21 unspecified role unspecified role bait bait physical association physical association S1K FoxM1B binding ability to beta-catenin is higher than that of FoxM1C. foxm1-myc 9 25 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q08050:p.Arg286_His287delinsAlaAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain RH AA Thu Jul 07 12:18:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg286his287ala Fri Jul 22 11:05:47 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg286ala,his287ala' Wed May 02 19:23:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_his286-287ala_ala' Tue Jun 30 16:24:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg286_His287delinsAlaAla' 27 unspecified role unspecified role prey prey promoter_foxm1_binding_elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S3C FoxM1 binds to the region -202 to +41 (from TSS) of the c-myc-promoter, which contains two FoxM1 binding elements. foxm1-myc-1 9 27 unspecified role unspecified role prey prey promoter_b_catenin/tcf_binding_element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S3D FoxM1 binds to the region -749 to -462 (from TSS) of the c-myc-promoter, which contains a beta-catenin /TCF binding element. The R286A/H287A mutant also bound to this genomic region. ctnnb1-foxm1-5 2 26 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig S4K FoxM1 and beta-catenin levels were highly elevated in the brain tumors. ctnnb1-foxm1-6 13 25 unspecified role unspecified role neutral component neutral component 26 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig S5E ctnnb1-foxm1-7 18 24 unspecified role unspecified role neutral component neutral component 28 unspecified role unspecified role neutral component neutral component colocalization colocalization S5G tcf7l2-foxm1-2 8 20 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 6J Depletion of beta-catenin by a siRNA or genetic deletion reduced or abolished the association between FoxM1 and TCF4. tcf7l2-ctnnb1-11 1 20 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role prey prey physical association physical association 6I Binding between β-catenin and TCF4 was decreased upon FoxM1 depletion.