I2D I2D And-1 is required for the stability of histone acetyltransferase Gcn5. And-1 is required for the stability of histone acetyltransferase Gcn5. Oncogene (0950-9232) 2012 imex curation Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu,bcmwxz@gwumc.edu 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu wz6812@gwu.edu,bcmwxz@gwumc.edu Accepted 2013-JAN-24 by MGT 04-Feb-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database And-1 is required for the stability of histone acetyltransferase Gcn5. And-1 is required for the stability of histone acetyltransferase Gcn5. Oncogene (0950-9232) 2012 imex curation Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu,bcmwxz@gwumc.edu 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu wz6812@gwu.edu,bcmwxz@gwumc.edu Accepted 2013-JAN-24 by MGT 04-Feb-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database And-1 is required for the stability of histone acetyltransferase Gcn5. And-1 is required for the stability of histone acetyltransferase Gcn5. Oncogene (0950-9232) 2012 imex curation Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu,bcmwxz@gwumc.edu 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu wz6812@gwu.edu,bcmwxz@gwumc.edu Accepted 2013-JAN-24 by MGT 04-Feb-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database And-1 is required for the stability of histone acetyltransferase Gcn5. And-1 is required for the stability of histone acetyltransferase Gcn5. Oncogene (0950-9232) 2012 imex curation Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu,bcmwxz@gwumc.edu 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 confocal microscopy confocal microscopy immunostaining immunostaining Li Y., Jaramillo-Lambert A.N., Yang Y., Williams R., Lee N.H., Zhu W. Cancer - Interactions investigated in the context of cancer Author confirms that Flag-Gcn5 is human. wz6812@gwu.edu wz6812@gwu.edu,bcmwxz@gwumc.edu Accepted 2013-JAN-24 by MGT 04-Feb-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database wdhd1_human WD repeat and HMG-box DNA-binding protein 1 WDHD1 AND1 Acidic nucleoplasmic DNA-binding protein 1 protein protein human Homo sapiens Human MPATRKPMRYGHTEGHTEVCFDDSGSFIVTCGSDGDVRIWEDLDDDDPKFINVGEKAYSCALKSGKLVTAVSNNTIQVHTFPEGVPDGILTRFTTNANHVVFNGDGTKIAAGSSDFLVKIVDVMDSSQQKTFRGHDAPVLSLSFDPKDIFLASASCDGSVRVWQISDQTCAISWPLLQKCNDVINAKSICRLAWQPKSGKLLAIPVEKSVKLYRRESWSHQFDLSDNFISQTLNIVTWSPCGQYLAAGSINGLIIVWNVETKDCMERVKHEKGYAICGLAWHPTCGRISYTDAEGNLGLLENVCDPSGKTSSSKVSSRVEKDYNDLFDGDDMSNAGDFLNDNAVEIPSFSKGIINDDEDDEDLMMASGRPRQRSHILEDDENSVDISMLKTGSSLLKEEEEDGQEGSIHNLPLVTSQRPFYDGPMPTPRQKPFQSGSTPLHLTHRFMVWNSIGIIRCYNDEQDNAIDVEFHDTSIHHATHLSNTLNYTIADLSHEAILLACESTDELASKLHCLHFSSWDSSKEWIIDLPQNEDIEAICLGQGWAAAATSALLLRLFTIGGVQKEVFSLAGPVVSMAGHGEQLFIVYHRGTGFDGDQCLGVQLLELGKKKKQILHGDPLPLTRKSYLAWIGFSAEGTPCYVDSEGIVRMLNRGLGNTWTPICNTREHCKGKSDHYWVVGIHENPQQLRCIPCKGSRFPPTLPRPAVAILSFKLPYCQIATEKGQMEEQFWRSVIFHNHLDYLAKNGYEYEESTKNQATKEQQELLMKMLALSCKLEREFRCVELADLMTQNAVNLAIKYASRSRKLILAQKLSELAVEKAAELTATQVEEEEEEEDFRKKLNAGYSNTATEWSQPRFRNQVEEDAEDSGEADDEEKPEIHKPGQNSFSKSTNSSDVSAKSGAVTFSSQGRVNPFKVSASSKEPAMSMNSARSTNILDNMGKSSKKSTALSRTTNNEKSPIIKPLIPKPKPKQASAASYFQKRNSQTNKTEEVKEENLKNVLSETPAICPPQNTENQRPKTGFQMWLEENRSNILSDNPDFSDEADIIKEGMIRFRVLSTEERKVWANKAKGETASEGTEAKKRKRVVDESDETENQEEKAKENLNLSKKQKPLDFSTNQKLSAFAFKQE kat2a_human Histone acetyltransferase KAT2A Histone acetyltransferase GCN5 STAF97 General control of amino acid synthesis protein 5-like 2 GCN5L2 Lysine acetyltransferase 2A KAT2A GCN5 Histone succinyltransferase KAT2A Histone glutaryltransferase KAT2A protein protein human Homo sapiens Human MAEPSQAPTPAPAAQPRPLQSPAPAPTPTPAPSPASAPIPTPTPAPAPAPAAAPAGSTGTGGPGVGSGGAGSGGDPARPGLSQQQRASQRKAQVRGLPRAKKLEKLGVFSACKANETCKCNGWKNPKPPTAPRMDLQQPAANLSELCRSCEHPLADHVSHLENVSEDEINRLLGMVVDVENLFMSVHKEEDTDTKQVYFYLFKLLRKCILQMTRPVVEGSLGSPPFEKPNIEQGVLNFVQYKFSHLAPRERQTMFELSKMFLLCLNYWKLETPAQFRQRSQAEDVATYKVNYTRWLCYCHVPQSCDSLPRYETTHVFGRSLLRSIFTVTRRQLLEKFRVEKDKLVPEKRTLILTHFPKFLSMLEEEIYGANSPIWESGFTMPPSEGTQLVPRPASVSAAVVPSTPIFSPSMGGGSNSSLSLDSAGAEPMPGEKRTLPENLTLEDAKRLRVMGDIPMELVNEVMLTITDPAAMLGPETSLLSANAARDETARLEERRGIIEFHVIGNSLTPKANRRVLLWLVGLQNVFSHQLPRMPKEYIARLVFDPKHKTLALIKDGRVIGGICFRMFPTQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHNILYFLTYADEYAIGYFKKQGFSKDIKVPKSRYLGYIKDYEGATLMECELNPRIPYTELSHIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKEGVRQIPVESVPGIRETGWKPLGKEKGKELKDPDQLYTTLKNLLAQIKSHPSAWPFMEPVKKSEAPDYYEVIRFPIDLKTMTERLRSRYYVTRKLFVADLQRVIANCREYNPPDSEYCRCASALEKFFYFKLKEGGLIDK DIP protein Q92830 original sequence version: 156 DIP protein Q92830 original sequence version: 158 728CC8ACF08600EA wdhd1-kat2a 1 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 1d kat2a-wdhd1 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 1d wdhd1-kat2a-1 3 5 unspecified role unspecified role bait bait full-length region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association 1e None of the And-1 deletion constructs showed any binding to Gcn5 - only the full length molecule showed binding. wdhd1-kat2a-2 2 5 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role prey prey purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction 1f kat2a-wdhd1-1 4 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 2a Gcn5 and And-1 colocalize during most of the cell cycle. During telophase however, And-1 reassociates with chromatin earlier than Gcn5.