I2D I2D An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. Oncogene (0950-9232) 2011 imex curation Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer m.seckl@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer m.seckl@imperial.ac.uk Accepted 2011-DEC-14 by ORCHARD Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. Oncogene (0950-9232) 2011 imex curation Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer m.seckl@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer Author confirms that all proteins used are human. The recombinant ribosomal S6 protein (P62753) used in the kinase assay is a C-term GST fusion recombinant S6 protein (amino acid 218 to 249). m.seckl@imperial.ac.uk Accepted 2011-DEC-14 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. Oncogene (0950-9232) 2011 imex curation Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer m.seckl@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro protein kinase assay protein kinase assay de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer Author confirms that all proteins used are human. m.seckl@imperial.ac.uk Accepted 2011-DEC-14 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database ks6a1_human Ribosomal protein S6 kinase alpha-1 RPS6KA1 RSK1 90 kDa ribosomal protein S6 kinase 1 MAPKAPK1A Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a protein protein human Homo sapiens Human MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL DIP protein Q15418 original sequence version: 111 DIP protein Q15418 original sequence version: 85 765731A4442A53DF vasp_human Vasodilator-stimulated phosphoprotein VASP protein protein human Homo sapiens Human MSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSP DIP protein P50552 original sequence version: 88 17634B8134DEBF59 rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK DIP protein P62753 original sequence version: 72 DIP protein P62753 original sequence version: 68 DIP protein P62753 original sequence version: 123 A61E435884E636AE enah_human Protein enabled homolog ENAH MENA protein protein human Homo sapiens Human MSEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVGRKIQDHQVVINCAIPKGLKYNQATQTFHQWRDARQVYGLNFGSKEDANVFASAMMHALEVLNSQETGPTLPRQNSQLPAQVQNGPSQEELEIQRRQLQEQQRQKELERERLERERMERERLERERLERERLERERLEQEQLERERQERERQERLERQERLERQERLERQERLDRERQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPPGPPPPPPLPSTGPPPPPPPPPLPNQVPPPPPPPPAPPLPASGFFLASMSEDNRPLTGLAAAIAGAKLRKVSRMEDTSFPSGGNAIGVNSASSKTDTGRGNGPLPLGGSGLMEEMSALLARRRRIAEKGSTIETEQKEDKGEDSEPVTSKASSTSTPEPTRKPWERTNTMNGSKSPVISRRDSPRKNQIVFDNRSYDSLHRPKSTPLSQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQELSKSNTA BF3252F6FCD1988B rps6ka1-vasp 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 3c vasp-rps6ka1 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 3c rps6ka1-enah 1 4 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 3d rps6ka1-vasp-1 2 5 enzyme target enzyme target substrate substrate unspecified role unspecified role c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 4 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation 3e rps6-rps6ka1 2 6 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation 3e Author confirms protein identity. rps6ka1-vasp-2 3 5 enzyme target enzyme target substrate substrate unspecified role unspecified role 4 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation S5 VASP is phosphorylated on Thr278.