I2D I2D Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Oncogene (0950-9232) 2011 imex curation Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that all constructs are of human origin. sunyi@umich.edu 26-Jun-2011: Contacted by JYOTI. jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. Author confirms that all constructs are of human origin. sunyi@umich.edu Accepted 2011-JUL-2 by JYOTI. 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Oncogene (0950-9232) 2011 imex curation Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that all constructs are of human origin. sunyi@umich.edu 26-Jun-2011: Contacted by JYOTI. jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Accepted 2011-JUL-2 by JYOTI. 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Oncogene (0950-9232) 2011 imex curation Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that all constructs are of human origin. sunyi@umich.edu 26-Jun-2011: Contacted by JYOTI. jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Accepted 2011-JUL-2 by JYOTI. 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Oncogene (0950-9232) 2011 imex curation Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that all constructs are of human origin. sunyi@umich.edu 26-Jun-2011: Contacted by JYOTI. jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Accepted 2011-JUL-2 by JYOTI. 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Oncogene (0950-9232) 2011 imex curation Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that all constructs are of human origin. sunyi@umich.edu 26-Jun-2011: Contacted by JYOTI. jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Accepted 2011-JUL-2 by JYOTI. 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Oncogene (0950-9232) 2011 imex curation Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that all constructs are of human origin. sunyi@umich.edu 26-Jun-2011: Contacted by JYOTI. jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Accepted 2011-JUL-2 by JYOTI. 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database rs27l_human 40S ribosomal protein S27-like RPS27L Small ribosomal subunit protein eS27-like protein protein human Homo sapiens Human MPLARDLLHPSLEEEKKKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH 271CCACFBB269E38 mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP DIP protein Q00987 original sequence version: 93 DIP protein Q00987-1 original sequence version: 192 DIP protein Q00987 original sequence version: 88 DIP protein Q00987 original sequence version: 180 DIP protein Q00987 original sequence version: 188 DIP protein Q00987 original sequence version: 189 DIP protein Q00987 original sequence version: 115 DIP protein Q00987-1 original sequence version: null DIP protein Q00987 original sequence version: 149 DIP protein Q00987 original sequence version: 146 DIP protein Q00987 original sequence version: 140 DIP protein Q00987 original sequence version: 183 DIP protein Q00987 original sequence version: 145 DIP protein Q00987 original sequence version: 182 DIP protein Q00987 original sequence version: 151 DIP protein Q00987 original sequence version: 156 DIP protein Q00987-1 original sequence version: 160 DIP protein Q00987 original sequence version: 160 DIP protein Q00987 original sequence version: 168 DIP protein Q00987 original sequence version: 186 DIP protein Q00987 original sequence version: 206 F37CE163876BC983 rs27_human 40S ribosomal protein S27 RPS27 MPS1 Metallopan-stimulin 1 Small ribosomal subunit protein eS27 protein protein human Homo sapiens Human MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH DIP protein P42677 original sequence version: 153 242C4466AC8A8900 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 rs27l_mouse 40S ribosomal protein S27-like Rps27l protein protein mouse Mus musculus Mouse MPLARDLLHPSLEEEKKKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH mdm2-rps27-1 3 9 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 3a mdm2-rps27l 3 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 3a mdm2-rs27l 6 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. physical association physical association 3c Cell lysates were from p53/Mdm2 double-null MEF cells. rps27l-mdm2 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 4a rps27-mdm2 1 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 4a mdm2-rps27-1 1 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 4b mdm2-rps27l-1 1 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 4b mdm2-rps27l-2 4 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:54:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys446arg,cys475ser' Tue Jun 30 18:41:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys446Arg;Cys475Ser]' physical association physical association 4a mdm2-rps27-4 4 9 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain K R Fri Jul 22 11:04:31 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'cys475ser,lys446arg' Wed May 02 16:33:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys446arg,cys475ser' Tue Jun 30 17:33:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys446Arg;Cys475Ser]' physical association physical association 4a mdm2-tp53-5 4 8 unspecified role unspecified role bait bait Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:14:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys446arg,cys475ser' Tue Jun 30 17:09:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys446Arg;Cys475Ser]' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey physical association physical association 4c mdm2-tp53-6 2 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:55:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys446arg,cys475ser' Tue Jun 30 19:54:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys446Arg;Cys475Ser]' physical association physical association 4d mdm2-rps27-3 2 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 4d mdm2-rps27l-3 2 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:44:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys446arg,cys475ser' Tue Jun 30 19:40:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys446Arg;Cys475Ser]' 7 unspecified role unspecified role prey prey physical association physical association 4d mdm2-rps27l-4 5 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 8a