IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Cancer Cell (1535-6108) 2010 imex curation Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] Cells were grown in "light" or "heavy" SILAC culture medium, then lysed, mixed in 1:1 ratio and immunoprecipitated. Resulting samples were analyzed by multidimensional liquid chromatography coupled with tandem mass spectrometry (MudPIT). Any "light" peptide detected by MudPIT with no similar amount of "heavy" peptide detected, was considered as a contaminant. All peptide sequences given from IPI00181058.2 in Table S6 were mapped to UniProt entry P29590-1. Experimental details were supplied by the authors. jdlee@scripps.edu Accepted 2010-NOV-21 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Cancer Cell (1535-6108) 2010 imex curation Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu Accepted 2010-NOV-21 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Cancer Cell (1535-6108) 2010 imex curation Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] Authors state PML is of human origin. jdlee@scripps.edu Accepted 2010-NOV-21 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Cancer Cell (1535-6108) 2010 imex curation Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] Details of constructs used given by the authors. jdlee@scripps.edu Accepted 2010-NOV-21 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Cancer Cell (1535-6108) 2010 imex curation Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro competition binding competition binding primer specific pcr primer specific pcr Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] In vitro ATP-site competition binding assay against a panel of 402 diverse kinases, using immobilised biotinylated ligands that bind with high affinity to the ATP site of multiple kinases.[Fabian et al., 2005, Karaman et al., 2008]. Kinases that exhibited >90% displacement by XMD8-92 are annotated. The assay was performed by Ambit Biosciences; they have confirmed that the kinases used in this assay were human in origin. jdlee@scripps.edu Accepted 2010-NOV-21 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Cancer Cell (1535-6108) 2010 imex curation Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu Accepted 2010-NOV-21 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Pharmacological inhibition of BMK1 suppresses tumor growth through promyelocytic leukemia protein. Cancer Cell (1535-6108) 2010 imex curation Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] jdlee@scripps.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 competition binding competition binding sequence tag sequence tag identification sequence tag Yang Q., Deng X., Lu B., Cameron M., Fearns C., Patricelli MP., Yates JR., Gray NS., Lee JD. Apoptosis - Interactions involving proteins with a function related to apoptosis This wondrous paper :there is a long section about SILAC in the Expt. Proc. in the main paper - but I can find NO actual expt/results in either main paper or supplement that use it....(Have I missed it??)[reply in author email states that SILAC was used as in Experiment 3 - IP/Mass Spec.] KiNativ profiling (Patricelli et al., 2007);Biotinylated acyl phosphate probes (ATP or ADP) irreversibly react at the ATP binding sites of protein kinases. Resultant biotinylated peptide fragments from labeled proteomes are captured, sequenced and identified using mass spec. Direct competition between the probes and protein kinase inhibitors can be assessed to determine inhibitor potency and selectivity. jdlee@scripps.edu Accepted 2010-NOV-21 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation biotin_acyl-p_probe Biotinylated acyl phosphate probe (ATP or ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pi4kb_human Phosphatidylinositol 4-kinase beta NPIK PI4K92 PI4KIII PI4KB PIK4CB protein protein human Homo sapiens Human MGDTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM CAD8791729BB4308 p29590-1 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-I TRIM19alpha Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS D50968A977E34287 mp2k5_rat Dual specificity mitogen-activated protein kinase kinase 5 Map2k5 Mek5 Mkk5 Prkmk5 MAPK/ERK kinase 5 protein protein rat Rattus norvegicus (Rat) Rat MLWLALGPFRAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHTSPVVSDSLPSNSLKKSSAELRKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKIPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTSGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPLGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNATVVSMWVCRALEERRSQQGPP 1B1CC8B05789B90D ks6a3_human Ribosomal protein S6 kinase alpha-3 Insulin-stimulated protein kinase 1 MAP kinase-activated protein kinase 1b 90 kDa ribosomal protein S6 kinase 3 MAPKAPK1B Ribosomal S6 kinase 2 pp90RSK2 RPS6KA3 ISPK1 RSK2 protein protein human Homo sapiens Human MPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQLYAMKVLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMILKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNKLYRREIHPPFKPATGRPEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFVAITSDDESQAMQTVGVHSIVQQLHRNSIQFTDGYEVKEDIGVGSYSVCKRCIHKATNMEFAVKIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVYVVTELMKGGELLDKILRQKFFSEREASAVLFTITKTVEYLHAQGVVHRDLKPSNILYVDESGNPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPDDTPEEILARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL DIP protein P51812 original sequence version: 155 DIP protein P51812 original sequence version: 84 486AE8357CEAB6C8 ks6a1_human Ribosomal protein S6 kinase alpha-1 RPS6KA1 RSK1 90 kDa ribosomal protein S6 kinase 1 MAPKAPK1A Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a protein protein human Homo sapiens Human MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL DIP protein Q15418 original sequence version: 111 DIP protein Q15418 original sequence version: 85 765731A4442A53DF ack1_human Activated CDC42 kinase 1 ACK1 TNK2 Tyrosine kinase non-receptor protein 2 protein protein human Homo sapiens Human MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNPMDPPDLLSVELSTSRPPQHLGGVKKPTYDPVSEDQDPLSSDFKRLGLRKPGLPRGLWLAKPSARVPGTKASRGSGAEVTLIDFGEEPVVPALRPCAPSLAQLAMDACSLLDETPPQSPTRALPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDFEICSINSTLVGAGVPAGPSQGQTNYAFVPEQARPPPPLEDNLFLPPQGGGKPPSSAQTAEIFQALQQECMRQLQAPAGSPAPSPSPGGDDKPQVPPRVPIPPRPTRPHVQLSPAPPGEEETSQWPGPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPPRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRAGPCILPIVRDGKKVSSTHYYLLPERPSYLERYQRFLREAQSPEEPTPLPVPLLLPPPSTPAPAAPTATVRPMPQAALDPKANFSTNNSNPGARPPPPRATARLPQRGCPGDGPEAGRPADKIQMAMVHGVTTEECQAALQCHGWSVQRAAQYLKVEQLFGLGLRPRGECHKVLEMFDWNLEQAGCHLLGSWGPAHHKR DIP protein Q07912 original sequence version: 150 DIP protein Q07912 original sequence version: 117 74A1980665BC3E6B mk07_human Mitogen-activated protein kinase 7 MAPK7 BMK1 ERK5 PRKM7 Extracellular signal-regulated kinase 5 Big MAP kinase 1 protein protein human Homo sapiens Human MAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP 27729FE31658CE3B dclk1_human Serine/threonine-protein kinase DCLK1 DCAMKL1 DCDC3A KIAA0369 Doublecortin-like and CAM kinase-like 1 Doublecortin-like kinase 1 Doublecortin domain-containing protein 3A DCLK1 protein protein human Homo sapiens Human MSFGRDMELEHFDERDKAQRYSRGSRVNGLPSPTHSAHCSFYRTRTLQTLSSEKKAKKVRFYRNGDRYFKGIVYAISPDRFRSFEALLADLTRTLSDNVNLPQGVRTIYTIDGLKKISSLDQLVEGESYVCGSIEPFKKLEYTKNVNPNWSVNVKTTSASRAVSSLATAKGSPSEVRENKDFIRPKLVTIIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGVVKRLYTLDGKQVMCLQDFFGDDDIFIACGPEKFRYQDDFLLDESECRVVKSTSYTKIASSSRRSTTKSPGPSRRSKSPASTSSVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRSSQHGGSSTSLASTKVCSSMDENDGPGEEVSEEGFQIPATITERYKVGRTIGDGNFAVVKECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAVQVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIATTALDKERQVFRRRRNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF D7B6D855099A315C dclk2_human Serine/threonine-protein kinase DCLK2 DCLK2 DCAMKL2 DCDC3B DCK2 Doublecortin-like and CAM kinase-like 2 Doublecortin-like kinase 2 Doublecortin domain-containing protein 3B CaMK-like CREB regulatory kinase 2 protein protein human Homo sapiens Human MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSSSGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYTKNINPNWSVNIKGGTSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKFRYAQDDFVLDHSECRVLKSSYSRSSAVKYSGSKSPGPSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAHGRSSSNVNGGPELDRCISPEGVNGNRCSESSTLLEKYKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMNTALDKEGQIFCSKHCQDSGRPGMEPISPVPPSVEEIPVPGEAVPAPTPPESPTPHPPPAAPGGERAGTWRRHRD 024F3223874AE83C fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH D8A4C15138AB0243 tnk1_human Non-receptor tyrosine-protein kinase TNK1 TNK1 CD38 negative kinase 1 protein protein human Homo sapiens Human MLPEAGSLWLLKLLRDIQLAQFYWPILEELNVTRPEHFDFVKPEDLDGIGMGRPAQRRLSEALKRLRSGPKSKNWVYKILGGFAPEHKEPTLPSDSPRHLPEPEGGLKCLIPEGAVCRGELLGSGCFGVVHRGLWTLPSGKSVPVAVKSLRVGPEGPMGTELGDFLREVSVMMNLEHPHVLRLHGLVLGQPLQMVMELAPLGSLHARLTAPAPTPPLLVALLCLFLRQLAGAMAYLGARGLVHRDLATRNLLLASPRTIKVADFGLVRPLGGARGRYVMGGPRPIPYAWCAPESLRHGAFSSASDVWMFGVTLWEMFSGGEEPWAGVPPYLILQRLEDRARLPRPPLCSRALYSLALRCWAPHPADRPSFSHLEGLLQEAGPSEACCVRDVTEPGALRMETGDPITVIEGSSSFHSPDSTIWKGQNGRTFKVGSFPASAVTLADAGGLPATRPVHRGTPARGDQHPGSIDGDRKKANLWDAPPARGQRRNMPLERMKGISRSLESVLSLGPRPTGGGSSPPEIRQARAVPQGPPGLPPRPPLSSSSPQPSQPSRERLPWPKRKPPHNHPMGMPGARKAAALSGGLLSDPELQRKIMEVELSVHGVTHQECQTALGATGGDVVSAIRNLKVDQLFHLSSRSRADCWRILEHYQWDLSAASRYVLARP AA7FBEF6CC778181 plk4_human Serine/threonine-protein kinase PLK4 Polo-like kinase 4 Serine/threonine-protein kinase Sak Serine/threonine-protein kinase 18 PLK4 SAK STK18 protein protein human Homo sapiens Human MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLRKTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQECVFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQEYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKSPKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMDHANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMHSAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH DIP protein O00444 original sequence version: 88 DIP protein O00444-1 original sequence version: 93 DIP protein O00444 original sequence version: 145 DIP protein O00444-1 original sequence version: 145 DIP protein O00444 original sequence version: 132 DIP protein O00444-1 original sequence version: 132 DIP protein O00444 original sequence version: 135 4D56F5FD983211A6 pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS DIP protein P29590 original sequence version: 188 DIP protein P29590 original sequence version: 195 DIP protein P29590-1 original sequence version: 187 D50968A977E34287 p42356-2 Phosphatidylinositol 4-kinase alpha PI4KA PIK4 PIK4CA Phosphatidylinositol 4-Kinase III alpha PI4K97 protein protein human Homo sapiens Human MREMAGAWHMTVEQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY F519168FA721644E xmd8-92 XMD8-92 a kinase inhibitor small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) bmk1-pml 6 21 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Fig 2A. pml-bmk 6 15 unspecified role unspecified role prey prey 21 unspecified role unspecified role bait bait physical association physical association Fig 2B. bmk1-pml-2 2 21 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Fig 2B. pml-bmk-1 2 15 unspecified role unspecified role prey prey 21 unspecified role unspecified role bait bait physical association physical association Fig 2B, S3B. PML phosphorylation (S403/T409) by BMK1 in vivo was demonstrated (Figure S3). This phosphorylation was decreased by addition of XMD8-92, a small-molecule inhibitor of the kinase activity of BMK1. I cannot enter the comment above as a PTM - as the manual states the term "resultant-ptm" should only be used for enzymatic reaction or child of. bmk1-pml-3 1 10 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain physical association physical association TABLE S6. IPI00181058.2 for PML, IPI00219601.2 for MAPK7. bmk1-pml-4 3 15 enzyme enzyme neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 21 enzyme target enzyme target substrate substrate neutral component neutral component P29590:p.Ser403Ala Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:45:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser403ala' Tue Jun 30 15:45:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser403Ala' P29590:p.Thr409Ala Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain T A Wed May 02 18:18:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr409ala' Tue Jun 30 19:10:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr409Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation Fig 3B. Phosphorylation of PML pml-bmk-2 4 11 unspecified role unspecified role ancillary ancillary Q62862:p.[Ser311Asp;Thr315Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain T D Wed May 02 20:02:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser311asp,thr315asp' Tue Jun 30 19:00:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser311Asp;Thr315Asp]' c terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 15 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 21 unspecified role unspecified role neutral component neutral component n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig 4A, 4B. Activated BMK1 (activated by a dominant active form of MEK5, MEK5D) is colocalized with PML in the PML-NBs. The kinase region, but not the nonkinase region, of BMK1 is sufficient for the colocalization of BMK1 and PML in PML-NBs. MEK5D: the correct amino acid nos of the mutations eventually found in Kamakura, JBC 1999. xmd8-92_bmk1 5 15 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role bait bait physical association physical association TABLE S1. Kinases that exhibited >90% displacement by XMD8-92 were determined to be BMK1, DCAMKL1, DCAMKL2, TNK1, and PLK4. XMD8-92 exhibited the greatest affinity toward BMK1 with a measured dissociation constant (KD) of 80 nM. This assay was NOT done by the authors of this paper, judging from their response to my query regarding species of molecules, quote- "I think it should be Human according to Ambit Biosciences" (Ambit is stated in Supplement at the top where TABLE S2 is listed). So I did not think there was much point asking the authors... mapk7_probe_xmd8-92 7 15 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 23 competitor competitor competitor prey prey 8 unspecified role unspecified role prey prey physical association physical association XMD8-92 was was found to be about 10-fold more selective for BMK1 with an IC50 of 1.5 uM. TABLE S2. xmd8-92_dclk1 5 16 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role bait bait physical association physical association TABLE S1. Kinases that exhibited >90% displacement by XMD8-92 were determined to be BMK1, DCAMKL1, DCAMKL2, TNK1, and PLK4. This assay was NOT done by the authors of this paper, judging from their response to my query regarding species of molecules, quote- "I think it should be Human according to Ambit Biosciences" (Ambit is stated in Supplement at the top where TABLE S2 is listed). So I did not think there was much point asking the authors... xmd8-92_dclk2 5 17 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role bait bait physical association physical association TABLE S1. Kinases that exhibited >90% displacement by XMD8-92 were determined to be BMK1, DCAMKL1, DCAMKL2, TNK1, and PLK4. I cannot determine from Fabian or Karaman papers what the BAIT used was - possibly several. This assay was NOT done by the authors of this paper, judging from their response to my query regarding species of molecules, quote- "I think it should be Human according to Ambit Biosciences" (Ambit is stated in Supplement at the top where TABLE S2 is listed). So I did not think there was much point asking the authors... xmd8-92_tnk1 5 19 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role bait bait physical association physical association TABLE S1. Kinases that exhibited >90% displacement by XMD8-92 were determined to be BMK1, DCAMKL1, DCAMKL2, TNK1, and PLK4. I cannot determine from Fabian or Karaman papers what the BAIT used was - possibly several. This assay was NOT done by the authors of this paper, judging from their response to my query regarding species of molecules, quote- "I think it should be Human according to Ambit Biosciences" (Ambit is stated in Supplement at the top where TABLE S2 is listed). So I did not think there was much point asking the authors... xmd8-92_plk4 5 20 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role bait bait physical association physical association TABLE S1. Kinases that exhibited >90% displacement by XMD8-92 were determined to be BMK1, DCAMKL1, DCAMKL2, TNK1, and PLK4. I cannot determine from Fabian or Karaman papers what the BAIT used was - possibly several. This assay was NOT done by the authors of this paper, judging from their response to my query regarding species of molecules, quote- "I think it should be Human according to Ambit Biosciences" (Ambit is stated in Supplement at the top where TABLE S2 is listed). So I did not think there was much point asking the authors... tnk1_probe_xmd8-92 7 8 unspecified role unspecified role prey prey 23 competitor competitor competitor prey prey 19 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain physical association physical association For TNK1, IC50 = 10 uM. TABLE S2. tnk2_probe_xmd8-92 7 14 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey 23 competitor competitor competitor prey prey physical association physical association For ACK1 (also known as TNK2) IC50 = 18 uM. TABLE S2. fak1_probe_xmd8-92 7 18 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 23 competitor competitor competitor prey prey 8 unspecified role unspecified role prey prey physical association physical association TABLE S2. ks6a1_probe_xmd8-92 7 13 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 23 competitor competitor competitor prey prey 8 unspecified role unspecified role prey prey physical association physical association TABLE S2. pi4kb_probe_xmb8-92 7 9 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey 23 competitor competitor competitor prey prey physical association physical association TABLE S2. ks6a3_probe_xmd8-92 7 12 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey 23 competitor competitor competitor prey prey physical association physical association TABLE S2. pi4ka_probe_xmd8-92 7 22 unspecified role unspecified role bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 23 competitor competitor competitor prey prey 8 unspecified role unspecified role prey prey physical association physical association TABLE S2.