IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ An ARF-independent c-MYC-activated tumor suppression pathway mediated by ribosomal protein-Mdm2 Interaction. An ARF-independent c-MYC-activated tumor suppression pathway mediated by ribosomal protein-Mdm2 Interaction. Cancer Cell (1535-6108) 2010 imex curation Macias E., Jin A., Deisenroth C., Bhat K., Mao H., Lindstrom MS., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The Mdm2 C305F mutation has been remapped to cys306phe I have amalgamated the 4 interactions above. I am not sure what you meant by "add entry for arf -/- cells" - as i thought we were not meant to add a separate Experiment for knockout cell lines - so I have added as a comment - as for the Act D - though it does not look very clear, to me. MEFs were isolated at embryonic day 13.5 (E13.5). Amino acid sequence numbering for Mouse MDM2 exons 7-12 was obtained from Iwakuma and Lozano, Molecular Cancer Research, 2003. ypzhang@med.unc.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions mouse-spleen Mus musculus spleen spleen spleen anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Macias E., Jin A., Deisenroth C., Bhat K., Mao H., Lindstrom MS., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis The Mdm2 C305F mutation has been remapped to cys306phe I have amalgamated the 4 interactions above. I am not sure what you meant by "add entry for arf -/- cells" - as i thought we were not meant to add a separate Experiment for knockout cell lines - so I have added as a comment - as for the Act D - though it does not look very clear, to me. MEFs were isolated at embryonic day 13.5 (E13.5). Amino acid sequence numbering for Mouse MDM2 exons 7-12 was obtained from Iwakuma and Lozano, Molecular Cancer Research, 2003. ypzhang@med.unc.edu Accepted 2010-DEC-1 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation An ARF-independent c-MYC-activated tumor suppression pathway mediated by ribosomal protein-Mdm2 Interaction. An ARF-independent c-MYC-activated tumor suppression pathway mediated by ribosomal protein-Mdm2 Interaction. Cancer Cell (1535-6108) 2010 imex curation Macias E., Jin A., Deisenroth C., Bhat K., Mao H., Lindstrom MS., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The Mdm2 C305F mutation has been remapped to cys306phe I have amalgamated the 4 interactions above. I am not sure what you meant by "add entry for arf -/- cells" - as i thought we were not meant to add a separate Experiment for knockout cell lines - so I have added as a comment - as for the Act D - though it does not look very clear, to me. MEFs were isolated at embryonic day 13.5 (E13.5). Amino acid sequence numbering for Mouse MDM2 exons 7-12 was obtained from Iwakuma and Lozano, Molecular Cancer Research, 2003. ypzhang@med.unc.edu 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Macias E., Jin A., Deisenroth C., Bhat K., Mao H., Lindstrom MS., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis The Mdm2 C305F mutation has been remapped to cys306phe The Mdm2 C305F mutation has been remapped to cys306phe I have amalgamated the 4 interactions above. I am not sure what you meant by "add entry for arf -/- cells" - as i thought we were not meant to add a separate Experiment for knockout cell lines - so I have added as a comment - as for the Act D - though it does not look very clear, to me. MEFs were isolated at embryonic day 13.5 (E13.5). Amino acid sequence numbering for Mouse MDM2 exons 7-12 was obtained from Iwakuma and Lozano, Molecular Cancer Research, 2003. ypzhang@med.unc.edu Accepted 2010-DEC-1 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation rl23_mouse 60S ribosomal protein L23 Rpl23 protein protein mouse Mus musculus Mouse MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA 807E14139B2EB0B5 rl5_mouse 60S ribosomal protein L5 Rpl5 protein protein mouse Mus musculus Mouse MGFVKVVKNKAYFKRYQVRFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVNGGEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNNVTPDMMEEMYKKAHAAIRENPVYEKKPKREVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES 30D74B9E66E17C65 rl11_mouse 60S ribosomal protein L11 Rpl11 protein protein mouse Mus musculus Mouse MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK 26EC965C9239774E mdm2_mouse E3 ubiquitin-protein ligase Mdm2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein Mdm2 RING-type E3 ubiquitin transferase Mdm2 protein protein mouse Mus musculus Mouse MCNTNMSVSTEGAASTSQIPASEQETLVRPKPLLLKLLKSVGAQNDTYTMKEIIFYIGQYIMTKRLYDEKQQHIVYCSNDLLGDVFGVPSFSVKEHRKIYAMIYRNLVAVSQQDSGTSLSESRRQPEGGSDLKDPLQAPPEEKPSSSDLISRLSTSSRRRSISETEENTDELPGERHRKRRRSLSFDPSLGLCELREMCSGGSSSSSSSSSESTETPSHQDLDDGVSEHSGDCLDQDSVSDQFSVEFEVESLDSEDYSLSDEGHELSDEDDEVYRVTVYQTGESDTDSFEGDPEISLADYWKCTSCNEMNPPLPSHCKRCWTLRENWLPDDKGKDKVEISEKAKLENSAQAEEGLDVPDGKKLTENDAKEPCAEEDSEEKAEQTPLSQESDDYSQPSTSSSIVYSSQESVKELKEETQDKDESVESSFSLNAIEPCVICQGRPKNGCIVHGKTGHLMSCFTCAKKLKKRNKPCPVCRQPIQMIVLTYFN DIP protein P23804 original sequence version: 108 DIP protein P23804 original sequence version: 117 DIP protein P23804 original sequence version: 90 DIP protein P23804 original sequence version: 109 DIP protein P23804 original sequence version: 145 DIP protein P23804 original sequence version: 129 DIP protein P23804 original sequence version: 120 DIP protein P23804 original sequence version: 166 4ABF489A82038DF4 p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 arf_mouse Tumor suppressor ARF Cdkn2a p19ARF Alternative reading frame Cyclin-dependent kinase inhibitor 2A protein protein mouse Mus musculus Mouse MGRRFLVTVRIQRAGRPLQERVFLVKFVRSRRPRTASCALAFVNMLLRLERILRRGPHRNPGPGDDDGQRSRSSSSAQLRCRFELRGPHYLLPPGARRSAGRLPGHAGGAARVRGSAGCARCLGSPAARLGPRAGTSRHRAIFAFRWVLFVFRWVVFVYRWERRPDRRA DIP protein Q64364 original sequence version: 97 DIP protein Q64364 original sequence version: 110 644505EFE1CBF478 mdm2-wt 2 8 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association Fig 2A, 6E. Mutant Mdm2 (cys306phe) did not interact with the L5 protein and had a weak interaction with the L11 protein. Actinomycin D Mutant Mdm2 (minus N terminal amino acids) does bind to p53 in Fig 2A, yet it does NOT bind p53 as shown in Fig 6E. In both Mdm2 mutant and p19 Arf knockout cell lines the addition of Actinomycin D increased the interaction of Mdm2 with L11 and p19Arf. In Fig 6E no interaction between Mdm2(wt) and p53 was observed in the absence of Act D, and no interaction between p53 and the mutant Mdm2 was noted at all. rl23_mdm2 2 3 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain P23804:p.Cys306Phe mutation mutation range range range range C F Wed May 02 17:45:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys306phe' Tue Jun 30 15:41:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys306Phe' physical association physical association Fig 2A, 2B. Mdm2C306F failed to bind L5 and L11, but was able to bind L23 and p53. mdm2-wt-1 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain P23804:p.Cys306Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain C F Wed May 02 14:27:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys306phe' Tue Jun 30 16:44:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys306Phe' physical association physical association The L11 in Eu-Myc;Mdm2C306F/C306F splenocytes, even though expressed to a higher level than in Eu-Myc;Mdm2+/+ splenocytes, showed very little interactions with Mdm2. Fig 5E.