IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Cancer Cell (1535-6108) 2010 Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer I cannot annotate Fig 3A: "GST_RBD" - no species of Rhotekin given, nor aa nos of the "limit Rho-binding domain"; nor Fig 8E(S4): Species of DBL not stated - Dr Eva 1994 ref does not state this either. NO reply from author. Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu 21-Dec-2010: Contacted by JYOTI. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells pull down pull down anti tag western anti tag western blot anti tag western Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer I cannot annotate Fig 3A: "GST_RBD" - no species of Rhotekin given, nor aa nos of the "limit Rho-binding domain"; nor Fig 8E(S4): Species of DBL not stated - Dr Eva 1994 ref does not state this either. NO reply from author. Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 jyoti Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Cancer Cell (1535-6108) 2010 Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer I cannot annotate Fig 3A: "GST_RBD" - no species of Rhotekin given, nor aa nos of the "limit Rho-binding domain"; nor Fig 8E(S4): Species of DBL not stated - Dr Eva 1994 ref does not state this either. NO reply from author. Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu 21-Dec-2010: Contacted by JYOTI. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells pull down pull down ms participant Identification by mass spectrometry ms participant Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer I cannot annotate Fig 3A: "GST_RBD" - no species of Rhotekin given, nor aa nos of the "limit Rho-binding domain"; nor Fig 8E(S4): Species of DBL not stated - Dr Eva 1994 ref does not state this either. NO reply from author. Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 jyoti Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Cancer Cell (1535-6108) 2010 Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer I cannot annotate Fig 3A: "GST_RBD" - no species of Rhotekin given, nor aa nos of the "limit Rho-binding domain"; nor Fig 8E(S4): Species of DBL not stated - Dr Eva 1994 ref does not state this either. NO reply from author. Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu 21-Dec-2010: Contacted by JYOTI. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer I cannot annotate Fig 3A: "GST_RBD" - no species of Rhotekin given, nor aa nos of the "limit Rho-binding domain"; nor Fig 8E(S4): Species of DBL not stated - Dr Eva 1994 ref does not state this either. NO reply from author. Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 968 5-[3-bromo-4-(dimethylamino)phenyl]-2,2-dimethyl-2,3,5,6-tetrahydrobenzo[a]phenanthridin-4(1H)-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) (5-[3-bromo-4-(dimethylamino)phenyl]-2,2-dimethyl-2,3,5,6-tetrahydrobenzo[a])is the name given in PMID: 20832749. (5-[3-bromo-4-(dimethylamino)phenyl]-2,2-dimethyl-2,3,5,6-tetrahydrobenzo[a])is the name given in PMID: 20832749. glsk_mouse Glutaminase kidney isoform, mitochondrial Gls Gls1 Kiaa0838 protein protein mouse Mus musculus Mouse MMRLRGSAMLRELLLRPPAAVGAVLRRAQPLGTLCRRPRGGSRPTAGLVAAARLHPWWGGGGRAKGPGAGGLSSSPSEILQELGKGGTPPQQQQQQQQQPGASPPAAPGPKDSPGETDAFGNSEGKEMVAAGDNKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSIGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDDGKGNQTVHKNLDGLL C46824C83E1D9639 active_968_biotin 3-bromo-4-(dimethylamino) benzaldehyde_biotin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) glutamine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gac_glu-968 3 4 inhibitor inhibitor neutral component neutral component 7 enzyme target enzyme target substrate substrate neutral component neutral component 5 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 5A. V max = 620 mol of glutamine hydrolyzed per minute per mol of enzyme. gac_glu-969 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5A. Is there a better way to enter the biotin-conjugate for the drug? gac_glu-970 2 5 unspecified role unspecified role prey prey region VSPESSDDTSTTVVYR identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait physical association physical association Fig S2A. Is there a better way to enter the biotin-conjugate for the drug?