I2D I2D K-Ras4B phosphorylation at Ser181 is inhibited by calmodulin and modulates K-Ras activity and function. K-Ras4B phosphorylation at Ser181 is inhibited by calmodulin and modulates K-Ras activity and function. Oncogene (0950-9232) 2010 imex curation Alvarez-Moya B., Lopez-Alcala C., Drosten M., Bachs O., Agell N. Cancer - Interactions investigated in the context of cancer Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. neusagell@ub.edu 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Alvarez-Moya B., Lopez-Alcala C., Drosten M., Bachs O., Agell N. Cancer - Interactions investigated in the context of cancer Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. neusagell@ub.edu Accepted 2011-FEB-14 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database K-Ras4B phosphorylation at Ser181 is inhibited by calmodulin and modulates K-Ras activity and function. K-Ras4B phosphorylation at Ser181 is inhibited by calmodulin and modulates K-Ras activity and function. Oncogene (0950-9232) 2010 imex curation Alvarez-Moya B., Lopez-Alcala C., Drosten M., Bachs O., Agell N. Cancer - Interactions investigated in the context of cancer Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. neusagell@ub.edu 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Alvarez-Moya B., Lopez-Alcala C., Drosten M., Bachs O., Agell N. Cancer - Interactions investigated in the context of cancer Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. neusagell@ub.edu Accepted 2011-FEB-14 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database K-Ras4B phosphorylation at Ser181 is inhibited by calmodulin and modulates K-Ras activity and function. K-Ras4B phosphorylation at Ser181 is inhibited by calmodulin and modulates K-Ras activity and function. Oncogene (0950-9232) 2010 imex curation Alvarez-Moya B., Lopez-Alcala C., Drosten M., Bachs O., Agell N. Cancer - Interactions investigated in the context of cancer Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. neusagell@ub.edu 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Alvarez-Moya B., Lopez-Alcala C., Drosten M., Bachs O., Agell N. Cancer - Interactions investigated in the context of cancer Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. Ras-binding domain of Raf-1 (RBD)-sepharose was used for the pull downs. neusagell@ub.edu Accepted 2011-FEB-14 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 p01116-2 GTPase KRas KRAS KRAS2 RASK2 K-Ras 2 Ki-Ras c-K-ras c-Ki-ras K-Ras4B protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM DIP protein P01116-2 original sequence version: 184 DIP protein P01116-2 original sequence version: 140 DIP protein P01116-2 original sequence version: 190 DIP protein P01116-2 original sequence version: 165 Variant in position: 152:V->G (in NS3). Variant in position: 153:D->V (in CFC2 and NS3, exhibits only minor alterations in its in vitro biochemical behavior compared to wild-type protein). Variant in position: 156:F->I (in NS3/CFC2). Variant in position: 156:F->L (found in a patient with Costello syndrome, exhibits an increase in intrinsic and guanine nucleotide exchange factor catalyzed nucleotide exchange in combination with an impaired GTPase-activating protein-stimulated GTP hydrolysis but functional in interaction with effectors). B1B6D189BB259861 calm_mouse Calmodulin Calm1 Calm Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc Cam protein protein mouse Mus musculus Mouse MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62204 original sequence version: 133 6B4BC3FCDE10727B k-ras-raf1 3 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01116-2:p.Gly12Val mutation increasing mutation increasing interaction mutation increasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G V Wed May 02 19:32:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val' Tue Jun 30 19:53:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val' P01116-2:p.Ser181Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:47:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser181ala' Tue Jun 30 18:21:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser181Ala' mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2a, 3a Binding occurs upon stimulation with 10ng/ml PDGF for 10 minutes Binding occurs upon activation by PMA and inhibition of CaM by W13. Binding also seen in NIH3T3WT8 cells upon stimulation with 100ng/ml EGF. Fig. 4b. k-ras-calm 1 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01116-2:p.Ser181Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:44:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser181asp' Tue Jun 30 16:28:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser181Asp' P01116-2:p.Gly12Val mutation increasing mutation increasing interaction mutation increasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G V Wed May 02 14:46:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val' Tue Jun 30 16:19:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val' mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells 6 unspecified role unspecified role prey prey physical association physical association Fig. 2c k-ras-raf1-3 2 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01116-2:p.Gly12Val mutation increasing mutation increasing interaction mutation increasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G V Wed May 02 19:57:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val' Tue Jun 30 19:29:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val' 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells physical association physical association Fig. 3b