I2D I2D Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Oncogene (0950-9232) 2010 imex curation Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Accepted 2011-MAR-25 by JYOTI Oncogene (0950-9232) 2010 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Oncogene (0950-9232) 2010 imex curation Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Extracts of endothelial progenitor cells isolated from peripheral blood mononuclear cells were used as the source of intergrin beta 1. Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Accepted 2011-MAR-25 by JYOTI Oncogene (0950-9232) 2010 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Oncogene (0950-9232) 2010 imex curation Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database mouse-h_end Mus musculus immortalized heart endothelial cells h_end H-End Murine endothelioma The h.end cell line was established from heart cells taken from a DBAI2xC57BV6 mouse fetus at day 13 gestation and immortalized by infection with the retrovirus vector N-TKmT containing PmT. This cell line was characterized as EC on the basis of expression od CD31, factor VIII, uptake of acetylated low-density lipoproteins and synthesis of platelet-activating factor and chemokines after stimulation with IL-1 or TNF. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Accepted 2011-MAR-25 by JYOTI Oncogene (0950-9232) 2010 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Oncogene (0950-9232) 2010 imex curation Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Accepted 2011-MAR-25 by JYOTI Oncogene (0950-9232) 2010 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. Oncogene (0950-9232) 2010 imex curation Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it jyoti Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Extracts of endothelial cells isolated and cultured from human umbilical veins within 4hr of delivery were used as the source of intergrin beta 1. Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Accepted 2011-MAR-25 by JYOTI Oncogene (0950-9232) 2010 jyoti Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database il3rb_human Cytokine receptor common subunit beta CSF2RB IL3RB IL5RB GM-CSF/IL-3/IL-5 receptor common beta subunit CDw131 protein protein human Homo sapiens Human MVLAQGLLSMALLALCWERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMWVLALIVIFLTIAVLLALRFCGIYGYRLRRKWEEKIPNPSKSHLFQNGSAELWPPGSMSAFTSGSPPHQGPWGSRFPELEGVFPVGFGDSEVSPLTIEDPKHVCDPPSGPDTTPAASDLPTEQPPSPQPGPPAASHTPEKQASSFDFNGPYLGPPHSRSLPDILGQPEPPQEGGSQKSPPPGSLEYLCLPAGGQVQLVPLAQAMGPGQAVEVERRPSQGAAGSPSLESGGGPAPPALGPRVGGQDQKDSPVAIPMSSGDTEDPGVASGYVSSADLVFTPNSGASSVSLVPSLGLPSDQTPSLCPGLASGPPGAPGPVKSGFEGYVELPPIEGRSPRSPRNNPVPPEAKSPVLNPGERPADVSPTSPQPEGLLVLQQVGDYCFLPGLGPGPLSLRSKPSSPGPGPEIKNLDQAFQVKKPPGQAVPQVPVIQLFKALKQQDYLSLPPWEVNKPGEVC 3398E37FDB8F393A itb1_mouse Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta Itgb1 protein protein mouse Mus musculus Mouse MNLQLVSWIGLISLICSVFGQTDKNRCLKANAKSCGECIQAGPNCGWCTNTTFLQEGMPTSARCDDLEALKKKGCQPSDIENPRGSQTIKKNKNVTNRSKGMAEKLRPEDITQIQPQQLLLKLRSGEPQKFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTDRGEFFNELVGQQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNVYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSGNSSNVIQLIIDAYNSLSSEVILENSKLPDGVTINYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITANKCPNKESETIKIKPLGFTEEVEVVLQFICKCNCQSHGIPASPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCRCRVCECYPNYTGSACDCSLDTGPCLASNGQICNGRGICECGACKCTDPKFQGPTCETCQTCLGVCAEHKECVQCRAFNKGEKKDTCAQECSHFNLTKVESREKLPQPVQVDPVTHCKEKDIDDCWFYFTYSVNGNNEAIVHVVETPDCPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P09055 original sequence version: 100 26788F7F0A168B56 itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C il3rb_mouse Cytokine receptor common subunit beta Csf2rb Aic2b Csf2rb1 Il3rb1 GM-CSF/IL-3/IL-5 receptor common beta subunit protein protein mouse Mus musculus Mouse MDQQMALTWGLCYMALVALCWGHGVTEAEETVPLKTLQCYNDYTNHIICSWADTEDAQGLINMTLYHQLEKKQPVSCELSEELMWSECPSSHRCVPRRCVIPYTRFSITNEDYYSFRPDSDLGIQLMVPLAQNVQPPLPKNVSISSSEDRFLLEWSVSLGDAQVSWLSSKDIEFEVAYKRLQDSWEDAYSLHTSKFQVNFEPKLFLPNSIYAARVRTRLSPGSSLSGRPSRWSPEVHWDSQPGDKAQPQNLQCFFDGIQSLHCSWEVWTQTTGSVSFGLFYRPSPVAPEEKCSPVVKEPPGASVYTRYHCSLPVPEPSAHSQYTVSVKHLEQGKFIMSYNHIQMEPPTLNLTKNRDSYSLHWETQKMAYSFIEHTFQVQYKKKSDSWEDSKTENLDRAHSMDLSQLEPDTSYCARVRVKPISNYDGIWSKWSEEYTWKTDWVMPTLWIVLILVFLILTLLLILRFGCVSVYRTYRKWKEKIPNPSKSLLFQDGGKGLWPPGSMAAFATKNPALQGPQSRLLAEQQGESYAHLEDNNVSPLTIEDPNIIRVPPSGPDTTPAASSESTEQLPNVQVEGPTPNRPRKQLPSFDFNGPYLGPPQSHSLPDLPDQLGSPQVGGSLKPALPGSLEYMCLPPGGQAQLVPLSQVMGQGQAMDVQCGSSLETSGSPSVEPKENPPVELSMEEQEARDNPVTLPISSGGPEGSMMASDYVTPGDPVLTLPTGPLSTSLGPSLGLPSAQSPSLCLKLPRVPSGSPALGPPGFEDYVELPPSVSQAAKSPPGHPAPPVASSPTVIPGEPREEVGPASPHPEGLLVLQQVGDYCFLPGLGPGSLSPHSKPPSPSLCSETEDLVQDLSVKKFPYQPMPQAPAIQFFKSLKHQDYLSLPPWDNSQSGKVC DIP protein P26955 original sequence version: 113 8E30ECB42C67F89A il-3rbc-integrinb1 4 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig. 1a, 4a il-3rbc-integrinb1-1 4 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig. 1a, 4a il-3rbc-integrinb1-2 1 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. S2b il-3rbc-integrinb1-3 2 6 unspecified role unspecified role bait bait chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell direct interaction direct interaction Fig. 2b il-3rbc-integrinb1-4 5 6 unspecified role unspecified role bait bait chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell direct interaction direct interaction Fig. S3a il-3rbc-integrinb1-5 3 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig. 4a il-3rbc-integrinb1-6 3 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 4a