IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-tov21g Homo sapiens TOV21G ovarian epithelial carcinoma cell line tov21g Human TOV21G ovarian epithelial carcinoma cell line with wt p53 gene. A highly tumourogenic cell line. TNF-alpha-producing. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-df09 Homo sapiens DF09 advanced ovarian cancer cell line df09 Human DF09 advanced ovarian cancer cell line Fresh primary tumor cells were derived from malignant ascites fluid obtained from patients with advanced ovarian cancer at the Dana-Farber Cancer Institute (DFCI). Greater than 80% epithelial tumor cells Fresh primary tumor cells were derived from malignant ascites fluid obtained from patients with advanced ovarian cancer at the Dana-Farber Cancer Institute (DFCI). Greater than 80% epithelial tumor cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-ovcar5 Homo sapiens OVCAR5 ovarian carcinoma cell line ovcar5 Human ovarian carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Conditioned medium from a variety of cell lines was added to OVCAR5 cells and the interaction of ErbB3 and p85 was observed. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-ov90 Homo sapiens OV90 epithelial ovarian cancer cell line ov90 Human OV-90 epithelial ovarian cancer cell line A spontaneously transformed epithelial ovarian epithelial cancer which harbors a deletion of an entire chromosome 3p arm. Tumor Stage: grade 3, stage IIIC. Disease: malignant papillary serous adenocarcinoma. Derived from metastatic site: ascites. ATCC number: CRL-11732. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-11732&Template=cellBiology A spontaneously transformed epithelial ovarian epithelial cancer which harbors a deletion of an entire chromosome 3p arm. Tumor Stage: grade 3, stage IIIC. Disease: malignant papillary serous adenocarcinoma. Derived from metastatic site: ascites. ATCC number: CRL-11732. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-fu_ov_1 Homo sapiens FU-OV-1 ovarian carcinoma cell line fu_ov_1 Human FU-OV-1 ovarian carcinoma (stage IIIc;, poorly differentiated serous papillary adenocarcinoma) Established post-hysterectomy from the tumor tissue of a 65-year-old Japanese woman with malignant ovarian carcinoma (stage: FIGO IIIc; histology: poorly differentiated serous papillary adenocarcinoma) in 1989. Morphology: epithelial-like adherent cells growing in monolayers. Human ovarian carcinoma established post-hysterectomy from the tumor tissue of a 65-year-old Japanese woman with malignant ovarian carcinoma (stage: FIGO IIIc; histology: poorly differentiated serous papillary adenocarcinoma) in 1989. Morphology: epithelial-like adherent cells growing in monolayers. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-df25 Homo sapiens DF25 advanced ovarian cancer cell line. df25 Human DF25 advanced ovarian cancer cell line Fresh primary tumor cells were derived from malignant ascites fluid obtained from patients with advanced ovarian cancer at the Dana-Farber Cancer Institute (DFCI). Greater than 80% epithelial tumor cells Fresh primary tumor cells were derived from malignant ascites fluid obtained from patients with advanced ovarian cancer at the Dana-Farber Cancer Institute (DFCI). Greater than 80% epithelial tumor cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-df14 Homo sapiens DF14 Ovarain cancer cell line df14 Human DF14 Ovarian cancer cells Primary ovarian cancer cells that demonstrated evidence of an NRG1-producing/activated-ErbB3 phenotype. Obtained from patients with advanced ovarian cancer at Dana-Farber Cancer Institute anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-ovcar432 Homo sapiens OVCAR432 ovarian cancer cell line ovcar432 Human OVCAR432 ovarian cancer cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-ovca420 Homo sapiens OVCA420 Ovarian cancer cell line ovca420 Human OVCA420 Ovarian carcinoma cell line This may also be known as OVCAR420. This cell line may also be known as OVCAR420. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-skov_3 Homo sapiens ovarian adenocarcinoma cell line skov_3 Human ovarian adenocarcinoma cell line Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-df26 Homo sapiens DF26 advanced ovarian cancer cell line df26 Human DF26 advanced ovarian cancer cell line Fresh primary tumor cells were derived from malignant ascites fluid obtained from patients with advanced ovarian cancer at the Dana-Farber Cancer Institute (DFCI). Greater than 80% epithelial tumor cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-ovcar8 Homo sapiens OVCAR8 ovarian adenocarcinoma cell line. ovcar8 Human ovarian adenocarcinoma cell line OVCAR 8 cells express the MIS type II receptor. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-ovcar433 Homo sapiens OVCAR433 Ovarian cancer cell line ovcar433 Human OVCAR433 ovarian cancer cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell (1535-6108) 2010 imex curation Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Cancer - Interactions investigated in the context of cancer Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 18 human-df20 Homo sapiens DF20 advanced ovarian cancer cell line df20 Human DF020 advanced ovarian cancer cell line Fresh primary tumor cells were derived from malignant ascites fluid obtained from patients with advanced ovarian cancer at the Dana-Farber Cancer Institute (DFCI). Greater than 80% epithelial tumor cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sheng Q., Liu X., Fleming E., Yuan K., Piao H., Chen J., Moustafa Z., Thomas RK., Greulich H., Schinzel A., Zaghlul S., Batt D., Ettenberg S., Meyerson M., Schoeberl B., Kung AL., Hahn WC., Drapkin R., Livingston DM., Liu JF. Synapse - Interactions of proteins with an established role in the presynapse. Experimental details for Figs 4B and 4D supplied by author. David_Livingston@dfci.harvard.edu Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 18 imex curation erbb3_human Receptor tyrosine-protein kinase erbB-3 Tyrosine kinase-type cell surface receptor HER3 ERBB3 HER3 Proto-oncogene-like protein c-ErbB-3 protein protein human Homo sapiens Human MRANDALQVLGLLFSLARGSEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGQTLVLIGKTHLTMALTVIAGLVVIFMMLGGTFLYWRGRRIQNKRAMRRYLERGESIEPLDPSEKANKVLARIFKETELRKLKVLGSGVFGTVHKGVWIPEGESIKIPVCIKVIEDKSGRQSFQAVTDHMLAIGSLDHAHIVRLLGLCPGSSLQLVTQYLPLGSLLDHVRQHRGALGPQLLLNWGVQIAKGMYYLEEHGMVHRNLAARNVLLKSPSQVQVADFGVADLLPPDDKQLLYSEAKTPIKWMALESIHFGKYTHQSDVWSYGVTVWELMTFGAEPYAGLRLAEVPDLLEKGERLAQPQICTIDVYMVMVKCWMIDENIRPTFKELANEFTRMARDPPRYLVIKRESGPGIAPGPEPHGLTNKKLEEVELEPELDLDLDLEAEEDNLATTTLGSALSLPVGTLNRPRGSQSLLSPSSGYMPMNQGNLGESCQESAVSGSSERCPRPVSLHPMPRGCLASESSEGHVTGSEAELQEKVSMCRSRSRSRSPRPRGDSAYHSQRHSLLTPVTPLSPPGLEEEDVNGYVMPDTHLKGTPSSREGTLSSVGLSSVLGTEEEDEDEEYEYMNRRRRHSPPHPPRPSSLEELGYEYMDVGSDLSASLGSTQSCPLHPVPIMPTAGTTPDEDYEYMNRQRDGGGPGGDYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT DIP protein P21860 original sequence version: 175 DIP protein P21860 original sequence version: 125 DIP protein P21860 original sequence version: 172 DIP protein P21860 original sequence version: 130 DIP protein P21860 original sequence version: 157 DIP protein P21860 original sequence version: 137 7201E7F66CA374BD erbb4_human Receptor tyrosine-protein kinase erbB-4 ERBB4 HER4 p180erbB4 Proto-oncogene-like protein c-ErbB-4 Tyrosine kinase-type cell surface receptor HER4 protein protein human Homo sapiens Human MKPATGLWVWVSLLVAAGTVQPSDSQSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLSFLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNLTLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHGDPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNLVTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTINQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRRFSRGRICIESCNLYDGEFREFENGSICVECDPQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADPDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHARTPLIAAGVIGGLFILVIVGLTFAVYVRRKSIKKKRALRRFLETELVEPLTPSGTAPNQAQLRILKETELKRVKVLGSGAFGTVYKGIWVPEGETVKIPVAIKILNETTGPKANVEFMDEALIMASMDHPHLVRLLGVCLSPTIQLVTQLMPHGCLLEYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLVHRDLAARNVLVKSPNHVKITDFGLARLLEGDEKEYNADGGKMPIKWMALECIHYRKFTHQSDVWSYGVTIWELMTFGGKPYDGIPTREIPDLLEKGERLPQPPICTIDVYMVMVKCWMIDADSRPKFKELAAEFSRMARDPQRYLVIQGDDRMKLPSPNDSKFFQNLLDEEDLEDMMDAEEYLVPQAFNIPPPIYTSRARIDSNRSEIGHSPPPAYTPMSGNQFVYRDGGFAAEQGVSVPYRAPTSTIPEAPVAQGATAEIFDDSCCNGTLRKPVAPHVQEDSSTQRYSADPTVFAPERSPRGELDEEGYMTPMRDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHNASNGPPKAEDEYVNEPLYLNTFANTLGKAEYLKNNILSMPEKAKKAFDNPDYWNHSLPPRSTLQHPDYLQEYSTKYFYKQNGRIRPIVAENPEYLSEFSLKPGTVLPPPPYRHRNTVV DIP protein Q15303 original sequence version: 163 DIP protein Q15303-1 original sequence version: 92 DIP protein Q15303-1 original sequence version: 127 DIP protein Q15303-1 original sequence version: 96 DIP protein Q15303 original sequence version: 96 5E4AE80985D88761 p85a_human Phosphatidylinositol 3-kinase regulatory subunit alpha PIK3R1 GRB1 Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha protein protein human Homo sapiens Human MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGSSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR DIP protein P27986 original sequence version: 181 DIP protein P27986 original sequence version: 184 DIP protein P27986-1 original sequence version: 135 DIP protein P27986 original sequence version: 135 DIP protein P27986-1 original sequence version: 145 DIP protein P27986-1 original sequence version: 147 DIP protein P27986 original sequence version: 191 B9DAD8416C33140F erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV DIP protein P04626 original sequence version: 198 DIP protein P04626 original sequence version: 122 DIP protein P04626 original sequence version: 206 DIP protein P04626 original sequence version: 195 DIP protein P04626 original sequence version: 150 DIP protein P04626 original sequence version: 179 DIP protein P04626 original sequence version: 146 DIP protein P04626 original sequence version: 147 39E9DFDA04DCF962 erbb4-erbb3 7 16 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association ErbB3 coimmunoprecipitated with both ErbB2 and ErbB4. Fig 4C. erbb2-erbb3 7 15 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Fig 4C ErbB3 coimmunoprecipitated with both ErbB2 and ErbB4. erbb3-erbb4 7 15 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association ErbB3 coimmunoprecipitated with both ErbB2 and ErbB4. Fig 4C. erbb3-p85-3 9 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A. erbb3-p85-5 12 17 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A. erbb3-p85-7 8 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A. erbb3-p85-8 13 17 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A. erbb3-p85-9 10 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A. erbb3-p85-10 1 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A. erbb3-p85-11 2 17 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association ErbB3 is expressed and activated in a subset of primary human ovarian cancer cells. The p85 subunit of PI3K was coimmunoprecipitated in cells that phosphorylate ErbB3 on Y1289. Fig 4B. erbb3-p85-12 7 17 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association ErbB3 is expressed and activated in a subset of primary human ovarian cancer cells. The p85 subunit of PI3K was coimmunoprecipitated in cells that phosphorylate ErbB3 on Y1289. Fig 4B. erbb3-p85-13 14 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association ErbB3 is expressed and activated in a subset of primary human ovarian cancer cells. The p85 subunit of PI3K was coimmunoprecipitated in cells that phosphorylate ErbB3 on Y1289. Fig 4B. erbb3-p85-14 12 17 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association ErbB3 is expressed and activated in a subset of primary human ovarian cancer cells. The p85 subunit of PI3K was coimmunoprecipitated in cells that phosphorylate ErbB3 on Y1289. Fig 4B. erbb3-p85-15 6 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association ErbB3 is expressed and activated in a subset of primary human ovarian cancer cells. The p85 subunit of PI3K was coimmunoprecipitated in cells that phosphorylate ErbB3 on Y1289. Fig 4B. erbb3-p85-16 11 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association ErbB3 is expressed and activated in a subset of primary human ovarian cancer cells. The p85 subunit of PI3K was coimmunoprecipitated in cells that phosphorylate ErbB3 on Y1289. Fig 4B. erbb3-p85-18 3 15 unspecified role unspecified role bait bait tyr1289 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association Culture medium from some of the primary ovarian tumor cell samples that express NRG1 mRNA (DF05, DF14) activated ErbB3 (and PI3K/AKT pathway) in OVCAR5 cells (which express but do not activate ErbB3). Fig 4D. Recombinant human NRG1-beta-1 ECD (extra cellular domain). erbb3-p85-20 5 15 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A. Weak interaction. erbb3-p85-21 4 15 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey physical association physical association Of 16 cell lines examined, ErbB3 was expressed and constitutively activated (indicated by phosphorylation of Y1289) in 9 and present but not activated in 6. Fig 2A.