IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. Cancer Cell (1535-6108) 2009 imex curation Xiao W., Hong H., Kawakami Y., Kato Y., Wu D., Yasudo H., Kimura A., Kubagawa H., Bertoli LF., Davis RS., Chau LA., Madrenas J., Hsia CC., Xenocostas A., Kipps TJ., Hennighausen L., Iwama A., Nakauchi H., Kawakami T. Cancer - Interactions investigated in the context of cancer no Fig 4F could be entered as a "Phosphatase assay" but the species of constructs is not stated. (The STAT5 is 'Human' but the species of the GST tagged proteins is not stated.)I cannot add Figs S9B and C, nor Fig S10 as the species of cDNA in construct is not stated. 10 ng/ml of IL-3 was added for 0-10 minutes. STAT5A has been entered throuhout, as no specific info is given in this paper as to whether STAT5A or STAT5B was used. toshi@liai.org 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 4 mouse-ba_f3 Mouse pro B cells IL-3 dependent mouse-baf3 EBI-6922762 ba_f3 Mouse pro B cells IL-3 dependent BA/F3 baf3 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiao W., Hong H., Kawakami Y., Kato Y., Wu D., Yasudo H., Kimura A., Kubagawa H., Bertoli LF., Davis RS., Chau LA., Madrenas J., Hsia CC., Xenocostas A., Kipps TJ., Hennighausen L., Iwama A., Nakauchi H., Kawakami T. Cancer - Interactions investigated in the context of cancer no Fig 4F could be entered as a "Phosphatase assay" but the species of constructs is not stated. (The STAT5 is 'Human' but the species of the GST tagged proteins is not stated.)I cannot add Figs S9B and C, nor Fig S10 as the species of cDNA in construct is not stated. 10 ng/ml of IL-3 was added for 0-10 minutes. STAT5A has been entered throuhout, as no specific info is given in this paper as to whether STAT5A or STAT5B was used. toshi@liai.org Accepted 2010-MAY-16 by ORCHARD. Cancer Cell (1535-6108) 2009 orchard Only protein-protein interactions 4 imex curation Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. Cancer Cell (1535-6108) 2009 imex curation Xiao W., Hong H., Kawakami Y., Kato Y., Wu D., Yasudo H., Kimura A., Kubagawa H., Bertoli LF., Davis RS., Chau LA., Madrenas J., Hsia CC., Xenocostas A., Kipps TJ., Hennighausen L., Iwama A., Nakauchi H., Kawakami T. Cancer - Interactions investigated in the context of cancer no Fig 4F could be entered as a "Phosphatase assay" but the species of constructs is not stated. (The STAT5 is 'Human' but the species of the GST tagged proteins is not stated.)I cannot add Figs S9B and C, nor Fig S10 as the species of cDNA in construct is not stated. 10 ng/ml of IL-3 was added for 0-10 minutes. STAT5A has been entered throuhout, as no specific info is given in this paper as to whether STAT5A or STAT5B was used. toshi@liai.org 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 4 mouse-spleen Mus musculus spleen spleen spleen anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiao W., Hong H., Kawakami Y., Kato Y., Wu D., Yasudo H., Kimura A., Kubagawa H., Bertoli LF., Davis RS., Chau LA., Madrenas J., Hsia CC., Xenocostas A., Kipps TJ., Hennighausen L., Iwama A., Nakauchi H., Kawakami T. Cancer - Interactions investigated in the context of cancer no Fig 4F could be entered as a "Phosphatase assay" but the species of constructs is not stated. (The STAT5 is 'Human' but the species of the GST tagged proteins is not stated.)I cannot add Figs S9B and C, nor Fig S10 as the species of cDNA in construct is not stated. Fig 4F could be entered as a "Phosphatase assay" but the species of constructs is not stated. (The STAT5 is 'Human' but the species of the GST tagged proteins is not stated.)I cannot add Figs S9B and C, nor Fig S10 as the species of cDNA in construct is not stated. 10 ng/ml of IL-3 was added for 0-10 minutes. STAT5A has been entered throuhout, as no specific info is given in this paper as to whether STAT5A or STAT5B was used. toshi@liai.org Accepted 2010-MAY-16 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. IL3 (ng/ml) Time (mins) Cancer Cell (1535-6108) 2009 orchard Only protein-protein interactions 4 imex curation ptn6_mouse Tyrosine-protein phosphatase non-receptor type 6 Ptpn6 Hcp Hcph Ptp1C Protein-tyrosine phosphatase 1C Hematopoietic cell protein-tyrosine phosphatase 70Z-SHP SH-PTP1 PTPTY-42 protein protein mouse Mus musculus Mouse MVRWFHRDLSGPDAETLLKGRGVPGSFLARPSRKNQGDFSLSVRVDDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGILQDRDGTIIHLKYPLNCSDPTSERWYHGHISGGQAESLLQAKGEPWTFLVRESLSQPGDFVLSVLNDQPKAGPGSPLRVTHIKVMCEGGRYTVGGSETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKSKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYVKNQLLGPDENSKTYIASQGCLDATVNDFWQMAWQENTRVIVMTTREVEKGRNKCVPYWPEVGTQRVYGLYSVTNSREHDTAEYKLRTLQISPLDNGDLVREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMESISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEIIQSQKGQESEYGNITYPPAVRSAHAKASRTSSKHKEEVYENVHSKSKKEEKVKKQRSADKEKNKGSLKRK DIP protein P29351 original sequence version: 154 DIP protein P29351 original sequence version: 102 DIP protein P29351 original sequence version: 117 DIP protein P29351 original sequence version: 164 DIP protein P29351 original sequence version: 134 CF17300D032638D2 plcb3_mouse 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Plcb3 Phosphoinositide phospholipase C-beta-3 Phospholipase C-beta-3 protein protein mouse Mus musculus Mouse MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL E055744A33C5F6B0 pic3b-stat5-ptn6 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey association association Pairwise interactions among PLC-b3, Ptpn6/SHP-1, and Stat5 were modestly induced upon IL-3 stimulation in nontransduced Ba/F cells and splenocytes from normal mice. STAT5A/B was also present, but it is not known which one it was. Fig 4D. Immunodepletion of STAT5 showed this molecule to be required for the interaction between Ptpn6 and Plcb3 to occur. ptn6-stat5-picb3 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait association association Pairwise interactions among PLC-B3, Ptpn6/SHP-1, and Stat5 were modestly induced upon IL-3 stimulation in nontransduced Ba/F cells and splenocytes from normal mice. STAT5A/B was also present, but it is not known which one it was. Fig 4D. Sup. Fig. 11B Immunodepletion of STAT5 showed this molecule to be required for the interaction between Ptpn6 and Plcb3 to occur. pic3b-ptn6-1 2 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association In WT mice, Ptpn6/SHP-1 was detected using PLC-B3 for IP. An 80% reduction in Stat5 expression did not affect the PLC-B3/SHP-1 interaction. Fig 4E. "polyI:C-treated Stat5fl/fl;Mx1-cre" - I can find no info in the paper or elsewhere asto what this might mean; presume the phrase entered in the comment above, is what is refered to by the expression in question... 0 0 picb3-stat5-ptn6 2 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association IL-3-inducible interactions between PLC-B3 and Stat5 were also shown in mouse splenocytes. STAT5A/B was present, but it is not known which one it was. Fig S9A. 10 0-3