IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell (0092-8674) 2009 imex curation Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it jyoti Only protein-protein interactions 10 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Accepted 2009-JUL-5 by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 10 imex curation A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell (0092-8674) 2009 imex curation Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it jyoti Only protein-protein interactions 10 human-hacat Homo sapiens immortalised keratinocytes hacat human immortalised keratinocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Accepted 2009-JUL-5 by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 10 imex curation A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell (0092-8674) 2009 imex curation Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it jyoti Only protein-protein interactions 10 human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Accepted 2009-JUL-5 by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 10 imex curation A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell (0092-8674) 2009 imex curation Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it jyoti Only protein-protein interactions 10 mouse-d3 Mouse D3 spindle cells d3 Mouse D3 spindle cell carcinoma of the skin cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Accepted 2009-JUL-5 by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 10 imex curation A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell (0092-8674) 2009 imex curation Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it jyoti Only protein-protein interactions 10 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Accepted 2009-JUL-5 by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 10 imex curation A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell (0092-8674) 2009 imex curation Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it jyoti Only protein-protein interactions 10 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Accepted 2009-JUL-5 by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 10 imex curation A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell (0092-8674) 2009 imex curation Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it jyoti Only protein-protein interactions 10 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Accepted 2009-JUL-5 by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 10 imex curation p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 o88898-2 Tumor protein 63 Tp63 P63 P73l Tp73l Trp63 Transformation-related protein 63 Tumor protein p73-like DeltaN-alpha protein protein mouse Mus musculus Mouse MLYLENNAQTQFSEPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSAKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSMQSQSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTMPEGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE Produced by alternative promoter usage. 622E24085B8BDCB7 smad3_human Mothers against decapentaplegic homolog 3 SMAD3 MADH3 SMAD family member 3 JV15-2 protein protein human Homo sapiens Human MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS DIP protein P84022 original sequence version: 57 DIP protein P84022 original sequence version: 112 DIP protein P84022-1 original sequence version: 146 DIP protein P84022-1 original sequence version: 111 46DF5E8B371321AC o88898-5 Tumor protein 63 Tp63 P63 P73l Tp73l Trp63 Transformation-related protein 63 Tumor protein p73-like TA*-gamma TA*p63gamma protein protein mouse Mus musculus Mouse MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSAKNGDAFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKHLLSACFRNELVEPRGEAPTQSDVFFRHSNPPNHSVYP Produced by alternative splicing of isoform 1. A90ED0C110C50EAD cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human smad2_mouse Mothers against decapentaplegic homolog 2 Smad2 Madh2 Madr2 Mad-related protein 2 SMAD family member 2 protein protein mouse Mus musculus Mouse MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTANTVDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS 31A2A36D463DB3E9 smad2_human Mothers against decapentaplegic homolog 2 SMAD2 MADH2 MADR2 Mad-related protein 2 SMAD family member 2 JV18-1 protein protein human Homo sapiens Human MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS DIP protein Q15796 original sequence version: 174 DIP protein Q15796 original sequence version: 100 DIP protein Q15796-1 original sequence version: 141 95406DB5FC0AA4C9 p63_human Tumor protein 63 TP63 KET P63 P73H P73L TP73L Transformation-related protein 63 Tumor protein p73-like p51 p40 Keratinocyte transcription factor KET Chronic ulcerative stomatitis protein protein protein human Homo sapiens Human MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE DIP protein Q9H3D4 original sequence version: 99 DIP protein Q9H3D4 original sequence version: 100 DIP protein Q9H3D4 original sequence version: 119 DIP protein Q9H3D4-1 original sequence version: 146 DIP protein Q9H3D4 original sequence version: 146 DIP protein Q9H3D4 original sequence version: 142 DIP protein Q9H3D4 original sequence version: 68 F66ECD45E87D9799 smad3_mouse Mothers against decapentaplegic homolog 3 Smad3 Madh3 SMAD family member 3 protein protein mouse Mus musculus Mouse MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS DIP protein Q8BUN5 original sequence version: 57 46DF5E8B371321AC p63_mouse Tumor protein 63 Tp63 P63 P73l Tp73l Trp63 Transformation-related protein 63 Tumor protein p73-like protein protein mouse Mus musculus Mouse MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSAKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSMQSQSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTMPEGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE 8DFF0284F247C68A tp53-tp63-1 2 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait P04637:p.[His179Tyr;Arg282Trp] mutation mutation certain certain sequence position certain certain certain sequence position certain H Y certain certain sequence position certain certain certain sequence position certain R W Wed May 02 20:32:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his179tyr,arg282trp' Tue Jun 30 17:01:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His179Tyr;Arg282Trp]' physical association physical association P04637 transfection Co-immunoprecipitation/Western blot analysis of HACAT cell lysates showing endogenous p63 bound to mutant-p53 Fig. 4A TGF-beta Pre-treatment with anti-SMAD2/3 or anti-TP53 siRNA inhibited this interaction. tp53-tp63-2 2 8 unspecified role unspecified role prey prey P04637:p.[His179Tyr;Arg282Trp] mutation mutation certain certain sequence position certain certain certain sequence position certain H Y certain certain sequence position certain certain certain sequence position certain R W Wed May 02 18:05:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his179tyr,arg282trp' Tue Jun 30 18:49:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His179Tyr;Arg282Trp]' 15 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association P04637 transfection Western blot analysis of endogenous proteins: p63 and mutant-p53 coimmunoprecipitated with Smad2 from HACAT cell lysates. Fig. 4B TGF-beta Pre-treatment with anti-SMAD2/3 or anti-TP63 siRNA inhibited this interaction. smad2-tp63-1 2 15 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association while the interaction of Smad2 with p63 is to a large extent independent from mutantp53 (Figure 4B, lane 3) Fig. 4B TGF-beta Cells were pre-treated with anti-TP53 siRNA. tp53-tp63-3 5 16 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 16:13:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 17:01:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' physical association physical association P04637 transfection Mutant-p53 immunopurification from null or p53R172H knockin MEFs reveals the formation of a TGFb- and Ras- dependent muant-p53/ Smad/p63 endogenous ternary complex. Fig. 4C TGF-beta TGF-beta induced the formation of a complex between p63, mutant-p53 and Smads only in presence of oncogenic Ras. TGF-beta receptor inhibitor SB431542 tp53-tp63-4 3 8 unspecified role unspecified role bait bait P04637:p.Arg280Lys mutation mutation certain certain sequence position certain certain certain sequence position certain R K Wed May 02 18:52:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg280lys' Tue Jun 30 16:36:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg280Lys' 15 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey physical association physical association Western blot analysis of endogenous proteins: p63 and mutant-p53 coimmunoprecipitated with Smad2 from HACAT cell lysates. Fig. 4E, Supp. Fig. 5B TGF-beta Pre-treatment with anti-SMAD2/3, anti CK1 epsilon/delta or anti-TP53 siRNA inhibited this interaction, as did inhibiting the RAS pathway with PD98059. smad3-tp63-1 6 10 unspecified role unspecified role bait bait m1 region required to bind necessary binding region required to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ta region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 47 43 direct interaction direct interaction Using different p63 isoforms, we dissected two independent Smad interaction surfaces in p63: the p63 N-terminal TA-domain contacts the MH1 domain of Smad3 whereas the p63 C-terminal alpha domain binds the Smad3-MH2 domain Exact coordinates of the SMAD3 M1 are not given in the text so have been inferred from the UniProtKB entry. Fig. 5A, Supp Fi. 7 smad3-tp63-2 6 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role bait bait m2 region required to bind necessary binding region required to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Using different p63 isoforms, we dissected two independent Smad interaction surfaces in p63: the p63 N-terminal TA-domain contacts the MH1 domain of Smad3 whereas the p63 C-terminal alpha domain binds the Smad3-MH2 domain Exact coordinates of the SMAD3 M2 are not given in the text so have been inferred from the UniProtKB entry. Fig. 5A, Supp Fi. 7 cdkn1a-1 1 12 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait association association ChIP analysis of p63 bound to its cognate responsive elements of the endogenous CDKN1A promoter Fig. 5D Interaction is inhibited by p53 (arg273his) but only when cells are treated with TGF-beta. tp53-tp63-5 4 17 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait P04637:p.Cys238Phe mutation mutation certain certain sequence position certain certain certain sequence position certain C F Wed May 02 17:21:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys238phe' Tue Jun 30 19:53:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys238Phe' physical association physical association Coimmunoprecipitation of p63 with mutant-p53 from D3 and D3S2 cell lysates Interaction also occurs in a metastatic sub-popultation of cells, D3S2. The interaction was not observed following treatment with anti SMAD2/3 siRNA. TGF-beta Fig. 6B tp53-tp63-6 7 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 20:31:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:00:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' 14 unspecified role unspecified role prey prey physical association physical association The formation of the mutant-p53/p63/RSmad complex requires N-terminal p53 phosphorylation by Ras/CK1 signaling Supp. Fig. 5C TGF-beta Mutation of the Ras/CK1-targeted phosphorylation sites of mutant-p53 abolished ternary complex formation.