IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Stabilization of N-Myc is a critical function of Aurora A in human neuroblastoma. Stabilization of N-Myc is a critical function of Aurora A in human neuroblastoma. Cancer Cell (1535-6108) 2009 imex curation Otto T., Horn S., Brockmann M., Eilers U., Schuttrumpf L., Popov N., Kenney AM., Schulte JH., Beijersbergen R., Christiansen H., Berwanger B., Eilers M. Cancer - Interactions investigated in the context of cancer martin.eilers@biozentrum.uni-wuerzburg.de Only protein-protein interactions 11 human-imr_5 Homo sapiens IMR-5:neuroblastoma cell line imr_5 Human IMR-5 Neuroblastoma cell line Morphology: neuroblastic-like, grown as monolayer. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Otto T., Horn S., Brockmann M., Eilers U., Schuttrumpf L., Popov N., Kenney AM., Schulte JH., Beijersbergen R., Christiansen H., Berwanger B., Eilers M. Cancer - Interactions investigated in the context of cancer The body of the paper states this experiment was carried out in IMR-32 cells, whereas the figure legend states IMR-5 cells were used. martin.eilers@biozentrum.uni-wuerzburg.de Accepted 2010-FEB-27 by ORCHARD. Cancer Cell (1535-6108) 2009 Only protein-protein interactions 11 imex curation Stabilization of N-Myc is a critical function of Aurora A in human neuroblastoma. Stabilization of N-Myc is a critical function of Aurora A in human neuroblastoma. Cancer Cell (1535-6108) 2009 imex curation Otto T., Horn S., Brockmann M., Eilers U., Schuttrumpf L., Popov N., Kenney AM., Schulte JH., Beijersbergen R., Christiansen H., Berwanger B., Eilers M. Cancer - Interactions investigated in the context of cancer martin.eilers@biozentrum.uni-wuerzburg.de Only protein-protein interactions 11 human-imr_32 Homo sapiens:Neuroblastoma cell line imr_32 Human IMR-32 Neuroblastoma Human neuroblastoma cell line established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old Caucasian boy in 1967. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Otto T., Horn S., Brockmann M., Eilers U., Schuttrumpf L., Popov N., Kenney AM., Schulte JH., Beijersbergen R., Christiansen H., Berwanger B., Eilers M. Cancer - Interactions investigated in the context of cancer martin.eilers@biozentrum.uni-wuerzburg.de Accepted 2010-FEB-27 by ORCHARD. Cancer Cell (1535-6108) 2009 Only protein-protein interactions 11 imex curation fbxw7_human F-box/WD repeat-containing protein 7 FBXW7 FBW7 FBX30 SEL10 F-box and WD-40 domain-containing protein 7 F-box protein FBX30 Archipelago homolog SEL-10 hCdc4 protein protein human Homo sapiens Human MNQELLSVGSKRRRTGGSLRGNPSSSQVDEEQMNRVVEEEQQQQLRQQEEEHTARNGEVVGVEPRPGGQNDSQQGQLEENNNRFISVDEDSSGNQEEQEEDEEHAGEQDEEDEEEEEMDQESDDFDQSDDSSREDEHTHTNSVTNSSSIVDLPVHQLSSPFYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK DIP protein Q969H0 original sequence version: 61 DIP protein Q969H0 original sequence version: 70 DIP protein Q969H0 original sequence version: 94 DIP protein Q969H0-1 original sequence version: 85 DIP protein Q969H0 original sequence version: 104 DIP protein Q969H0 original sequence version: 130 DIP protein Q969H0-1 original sequence version: 135 E4A357F76DFD8203 aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS DIP protein O14965 original sequence version: 181 DIP protein O14965 original sequence version: 146 DIP protein O14965 original sequence version: 124 DIP protein O14965 original sequence version: 160 DIP protein O14965 original sequence version: 138 DIP protein O14965 original sequence version: 98 125F3594834CD157 mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC DIP protein P04198 original sequence version: 157 560E885602E30DAD aura-mycn 2 5 unspecified role unspecified role bait bait thr-58 (possible) opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey physical association physical association Fig 6E. aurka-mycn-1 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig 6F. No MG-132 was added to the cells. a very weak interaction. fbxw7-mycn 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Supplementary Fig 6C. EndogenousN-Myc was present in Fbxw7 immunoprecipitates from IMR-32 cells (Figure S6C).