IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Cancer Cell (1535-6108) 2008 imex curation Yokoyama A., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. ayokoyam@ncc.go.jp 16-Oct-2008: Contacted by JYOTI. orchard Only protein-protein interactions 1 human-reh Homo sapiens REH B cell precursor leukemia cells reh Human B cell precursor leukemia REH cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yokoyama A., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. ayokoyam@ncc.go.jp Accepted 2008-SEP-28 by ORCHARD. 16-Oct-2008: Contacted by JYOTI. Cancer Cell (1535-6108) 2008 orchard Only protein-protein interactions 1 imex curation Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Cancer Cell (1535-6108) 2008 imex curation Yokoyama A., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. ayokoyam@ncc.go.jp 16-Oct-2008: Contacted by JYOTI. orchard Only protein-protein interactions 1 human-hb1119 Homo sapiens acute lymphoblastic leukemia cell line HB1119 hb1119 Human acute lymphoblastic leukemia cell line HB1119 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Yokoyama A., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. ayokoyam@ncc.go.jp Accepted 2008-SEP-28 by ORCHARD. 16-Oct-2008: Contacted by JYOTI. Cancer Cell (1535-6108) 2008 orchard Only protein-protein interactions 1 imex curation Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Cancer Cell (1535-6108) 2008 imex curation Yokoyama A., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. ayokoyam@ncc.go.jp 16-Oct-2008: Contacted by JYOTI. orchard Only protein-protein interactions 1 human-ml_2 Homo sapiens ML-2 acute myeloid leukemia-derived cell line ml_2 Human acute myeloid leukemia-derived cell line ML-2 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Yokoyama A., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply Wrote to authors to request info on species of origin of constructs 14-07-08 but no reply The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. The source of the MLL, MLL fusion protein, menin and LEDGF constructs could not be ascertained. Thus interactions involving these constructs could not be curated. ayokoyam@ncc.go.jp Accepted 2008-SEP-28 by ORCHARD. 16-Oct-2008: Contacted by JYOTI. Cancer Cell (1535-6108) 2008 orchard Only protein-protein interactions 1 imex curation hoxa9_human_gene Human HOXA9 gene gene gene human Homo sapiens Human meis1_human_gene gene gene human Homo sapiens Human kmt2a_human Histone-lysine N-methyltransferase 2A KMT2A ALL-1 ALL1 CXXC-type zinc finger protein 7 CXXC7 HRX HTRX MLL MLL1 Myeloid/lymphoid or mixed-lineage leukemia Myeloid/lymphoid or mixed-lineage leukemia protein 1 TRX1 Trithorax-like protein Zinc finger protein HRX Cysteine methyltransferase KMT2A protein protein human Homo sapiens Human MAHSCRWRFPARPGTTGGGGGGGRRGLGGAPRQRVPALLLPPGPPVGGGGPGAPPSPPAVAAAAAAAGSSGAGVPGGAAAASAASSSSASSSSSSSSSASSGPALLRVGPGFDAALQVSAAIGTNLRRFRAVFGESGGGGGSGEDEQFLGFGSDEEVRVRSPTRSPSVKTSPRKPRGRPRSGSDRNSAILSDPSVFSPLNKSETKSGDKIKKKDSKSIEKKRGRPPTFPGVKIKITHGKDISELPKGNKEDSLKKIKRTPSATFQQATKIKKLRAGKLSPLKSKFKTGKLQIGRKGVQIVRRRGRPPSTERIKTPSGLLINSELEKPQKVRKDKEGTPPLTKEDKTVVRQSPRRIKPVRIIPSSKRTDATIAKQLLQRAKKGAQKKIEKEAAQLQGRKVKTQVKNIRQFIMPVVSAISSRIIKTPRRFIEDEDYDPPIKIARLESTPNSRFSAPSCGSSEKSSAASQHSSQMSSDSSRSSSPSVDTSTDSQASEEIQVLPEERSDTPEVHPPLPISQSPENESNDRRSRRYSVSERSFGSRTTKKLSTLQSAPQQQTSSSPPPPLLTPPPPLQPASSISDHTPWLMPPTIPLASPFLPASTAPMQGKRKSILREPTFRWTSLKHSRSEPQYFSSAKYAKEGLIRKPIFDNFRPPPLTPEDVGFASGFSASGTAASARLFSPLHSGTRFDMHKRSPLLRAPRFTPSEAHSRIFESVTLPSNRTSAGTSSSGVSNRKRKRKVFSPIRSEPRSPSHSMRTRSGRLSSSELSPLTPPSSVSSSLSISVSPLATSALNPTFTFPSHSLTQSGESAEKNQRPRKQTSAPAEPFSSSSPTPLFPWFTPGSQTERGRNKDKAPEELSKDRDADKSVEKDKSRERDREREKENKRESRKEKRKKGSEIQSSSALYPVGRVSKEKVVGEDVATSSSAKKATGRKKSSSHDSGTDITSVTLGDTTAVKTKILIKKGRGNLEKTNLDLGPTAPSLEKEKTLCLSTPSSSTVKHSTSSIGSMLAQADKLPMTDKRVASLLKKAKAQLCKIEKSKSLKQTDQPKAQGQESDSSETSVRGPRIKHVCRRAAVALGRKRAVFPDDMPTLSALPWEEREKILSSMGNDDKSSIAGSEDAEPLAPPIKPIKPVTRNKAPQEPPVKKGRRSRRCGQCPGCQVPEDCGVCTNCLDKPKFGGRNIKKQCCKMRKCQNLQWMPSKAYLQKQAKAVKKKEKKSKTSEKKDSKESSVVKNVVDSSQKPTPSAREDPAPKKSSSEPPPRKPVEEKSEEGNVSAPGPESKQATTPASRKSSKQVSQPALVIPPQPPTTGPPRKEVPKTTPSEPKKKQPPPPESGPEQSKQKKVAPRPSIPVKQKPKEKEKPPPVNKQENAGTLNILSTLSNGNSSKQKIPADGVHRIRVDFKEDCEAENVWEMGGLGILTSVPITPRVVCFLCASSGHVEFVYCQVCCEPFHKFCLEENERPLEDQLENWCCRRCKFCHVCGRQHQATKQLLECNKCRNSYHPECLGPNYPTKPTKKKKVWICTKCVRCKSCGSTTPGKGWDAQWSHDFSLCHDCAKLFAKGNFCPLCDKCYDDDDYESKMMQCGKCDRWVHSKCENLSDEMYEILSNLPESVAYTCVNCTERHPAEWRLALEKELQISLKQVLTALLNSRTTSHLLRYRQAAKPPDLNPETEESIPSRSSPEGPDPPVLTEVSKQDDQQPLDLEGVKRKMDQGNYTSVLEFSDDIVKIIQAAINSDGGQPEIKKANSMVKSFFIRQMERVFPWFSVKKSRFWEPNKVSSNSGMLPNAVLPPSLDHNYAQWQEREENSHTEQPPLMKKIIPAPKPKGPGEPDSPTPLHPPTPPILSTDRSREDSPELNPPPGIEDNRQCALCLTYGDDSANDAGRLLYIGQNEWTHVNCALWSAEVFEDDDGSLKNVHMAVIRGKQLRCEFCQKPGATVGCCLTSCTSNYHFMCSRAKNCVFLDDKKVYCQRHRDLIKGEVVPENGFEVFRRVFVDFEGISLRRKFLNGLEPENIHMMIGSMTIDCLGILNDLSDCEDKLFPIGYQCSRVYWSTTDARKRCVYTCKIVECRPPVVEPDINSTVEHDENRTIAHSPTSFTESSSKESQNTAEIISPPSPDRPPHSQTSGSCYYHVISKVPRIRTPSYSPTQRSPGCRPLPSAGSPTPTTHEIVTVGDPLLSSGLRSIGSRRHSTSSLSPQRSKLRIMSPMRTGNTYSRNNVSSVSTTGTATDLESSAKVVDHVLGPLNSSTSLGQNTSTSSNLQRTVVTVGNKNSHLDGSSSSEMKQSSASDLVSKSSSLKGEKTKVLSSKSSEGSAHNVAYPGIPKLAPQVHNTTSRELNVSKIGSFAEPSSVSFSSKEALSFPHLHLRGQRNDRDQHTDSTQSANSSPDEDTEVKTLKLSGMSNRSSIINEHMGSSSRDRRQKGKKSCKETFKEKHSSKSFLEPGQVTTGEEGNLKPEFMDEVLTPEYMGQRPCNNVSSDKIGDKGLSMPGVPKAPPMQVEGSAKELQAPRKRTVKVTLTPLKMENESQSKNALKESSPASPLQIESTSPTEPISASENPGDGPVAQPSPNNTSCQDSQSNNYQNLPVQDRNLMLPDGPKPQEDGSFKRRYPRRSARARSNMFFGLTPLYGVRSYGEEDIPFYSSSTGKKRGKRSAEGQVDGADDLSTSDEDDLYYYNFTRTVISSGGEERLASHNLFREEEQCDLPKISQLDGVDDGTESDTSVTATTRKSSQIPKRNGKENGTENLKIDRPEDAGEKEHVTKSSVGHKNEPKMDNCHSVSRVKTQGQDSLEAQLSSLESSRRVHTSTPSDKNLLDTYNTELLKSDSDNNNSDDCGNILPSDIMDFVLKNTPSMQALGESPESSSSELLNLGEGLGLDSNREKDMGLFEVFSQQLPTTEPVDSSVSSSISAEEQFELPLELPSDLSVLTTRSPTVPSQNPSRLAVISDSGEKRVTITEKSVASSESDPALLSPGVDPTPEGHMTPDHFIQGHMDADHISSPPCGSVEQGHGNNQDLTRNSSTPGLQVPVSPTVPIQNQKYVPNSTDSPGPSQISNAAVQTTPPHLKPATEKLIVVNQNMQPLYVLQTLPNGVTQKIQLTSSVSSTPSVMETNTSVLGPMGGGLTLTTGLNPSLPTSQSLFPSASKGLLPMSHHQHLHSFPAATQSSFPPNISNPPSGLLIGVQPPPDPQLLVSESSQRTDLSTTVATPSSGLKKRPISRLQTRKNKKLAPSSTPSNIAPSDVVSNMTLINFTPSQLPNHPSLLDLGSLNTSSHRTVPNIIKRSKSSIMYFEPAPLLPQSVGGTAATAAGTSTISQDTSHLTSGSVSGLASSSSVLNVVSMQTTTTPTSSASVPGHVTLTNPRLLGTPDIGSISNLLIKASQQSLGIQDQPVALPPSSGMFPQLGTSQTPSTAAITAASSICVLPSTQTTGITAASPSGEADEHYQLQHVNQLLASKTGIHSSQRDLDSASGPQVSNFTQTVDAPNSMGLEQNKALSSAVQASPTSPGGSPSSPSSGQRSASPSVPGPTKPKPKTKRFQLPLDKGNGKKHKVSHLRTSSSEAHIPDQETTSLTSGTGTPGAEAEQQDTASVEQSSQKECGQPAGQVAVLPEVQVTQNPANEQESAEPKTVEEEESNFSSPLMLWLQQEQKRKESITEKKPKKGLVFEISSDDGFQICAESIEDAWKSLTDKVQEARSNARLKQLSFAGVNGLRMLGILHDAVVFLIEQLSGAKHCRNYKFRFHKPEEANEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYNPNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGVYRSPIHGRGLFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEVVDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTYDYKFPIEDASNKLPCNCGAKKCRKFLN DIP protein Q03164 original sequence version: 85 DIP protein Q03164-1 original sequence version: 137 DIP protein Q03164 original sequence version: 140 DIP protein Q03164-1 original sequence version: 141 DIP protein Q03164 original sequence version: 172 DIP protein Q03164-1 original sequence version: 190 DIP protein Q03164-1 original sequence version: 200 DIP protein Q03164 original sequence version: 153 DIP protein Q03164-1 original sequence version: 154 DIP protein Q03164-1 original sequence version: 196 DIP protein Q03164 original sequence version: 159 1150F37EAB1430D3 men1_human Menin MEN1 SCG2 protein protein human Homo sapiens Human MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPDPPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHIQSLFSFITGWSPVGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQTVNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPTASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL DIP protein O00255 original sequence version: 100 DIP protein O00255 original sequence version: 131 DDDF850EA5AB77B4 hoxa7_human_gene gene gene human Homo sapiens Human psip1_human PC4 and SFRS1-interacting protein PSIP1 DFS70 LEDGF PSIP2 Lens epithelium-derived growth factor Transcriptional coactivator p75/p52 Dense fine speckles 70 kDa protein CLL-associated antigen KW-7 protein protein human Homo sapiens Human MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEQQNKDEGKKPEVKKVEKKRETSMDSRLQRIHAEIKNSLKIDNLDVNRCIEALDELASLQVTMQQAQKHTEMITTLKKIRRFKVSQVIMEKSTMLYNKFKNMFLVGEGDSVITQVLNKSLAEQRQHEEANKTKDQGKKGPNKKLEKEQTGSKTLNGGSDAQDGNQPQHNGESNEDSKDNHEASTKKKPSSEERETEISLKDSTLDN DIP protein O75475 original sequence version: 124 DIP protein O75475-1 original sequence version: 108 DIP protein O75475-1 original sequence version: 113 DIP protein O75475 original sequence version: 113 4B653D02B1D0E174 men1-mll 1 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association 1 D LEDGF was detected in the endogenous MLL/menin complex in REH cells as well hoxa7-psip1 3 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association 2 A Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa9-psip1 3 9 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association 2 A Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa9-men1 3 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association 2 A Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa7-men1 3 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association 2 A Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa7-mll 3 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association 2 A Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa9-mll 3 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association 2 A Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa7-psip1-1 2 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa9-psip1-1 2 9 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa9-men1-1 2 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa7-men1-1 2 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa7-mll-1 2 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes hoxa9-mll-1 2 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes meis1-psip1 2 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes meis1-men1-2 2 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes meis1-mll-2 2 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey association association S2 Chromatin immunoprecipitation (ChIP) analyses demonstrated that LEDGF colocalizes with MLL/menin complexes at target sites within HOXA, MEIS1, and CDKI genes