IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. J. Biol. Chem. (0021-9258) 2007 imex curation Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu Accepted 2008-FEB-16 by DAVET. 01-Feb-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 davet Only protein-protein interactions imex curation Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. J. Biol. Chem. (0021-9258) 2007 imex curation Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions human-rao_3 Homo sapiens mammary epithelial cancer cell line RAO-3 rao_3 Human mammary epithelial cancer cell line RAO-3 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu Accepted 2008-FEB-16 by DAVET. 01-Feb-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 davet Only protein-protein interactions imex curation Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. J. Biol. Chem. (0021-9258) 2007 imex curation Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions human-rao_3 Homo sapiens mammary epithelial cancer cell line RAO-3 rao_3 Human mammary epithelial cancer cell line RAO-3 anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu Accepted 2008-FEB-16 by DAVET. 01-Feb-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 davet Only protein-protein interactions imex curation Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. Aldo-keto reductase family 1 B10 affects fatty acid synthesis by regulating the stability of acetyl-coa carboxylase-alpha in breast cancer cells. J. Biol. Chem. (0021-9258) 2007 imex curation Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions human-rao_3 Homo sapiens mammary epithelial cancer cell line RAO-3 rao_3 Human mammary epithelial cancer cell line RAO-3 confocal microscopy confocal microscopy immunostaining immunostaining Ma J., Yan R., Zu X., Cheng JM., Rao K., Liao DF., Cao D. Cancer - Interactions investigated in the context of cancer dcao@siumed.edu Accepted 2008-FEB-16 by DAVET. 01-Feb-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 davet Only protein-protein interactions imex curation ak1ba_human Aldo-keto reductase family 1 member B10 Aldose reductase-like ARL-1 Aldose reductase-related protein Small intestine reductase AKR1B10 AKR1B11 protein protein human Homo sapiens Human MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFNAEY 0C2FC0E798955A33 acaca_human Acetyl-CoA carboxylase 1 ACACA ACAC ACC1 ACCA Acetyl-Coenzyme A carboxylase alpha protein protein human Homo sapiens Human MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST DIP protein Q13085 original sequence version: 123 F1F0A518F8824FFC akr1b10-acaca 3 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 3 A, B and Fig. 4 Co-immunoprecipitation with AKR1B10 antibody and protein mass spectrometry analysis identified that AKR1B10 associates with acetyl-CoA carboxylase-α (ACCA), a rate-limiting enzyme of de novo fatty acid synthesis. akr1b10-acaca-1 2 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 5 In RAO-3 cells, AKR1B10 protein was specifically co-precipitated by ACCA antibody akr1b10-acaca-2 1 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey human-rao_3 Homo sapiens mammary epithelial cancer cell line RAO-3 rao_3 Human mammary epithelial cancer cell line RAO-3 physical association physical association 5 B ACCA was pulled down by histidine-tagged recombinant AKR1B10 protein acaca-akr1b10 4 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 6 intracellular fluorescent studies showed that ectopic AKR1B10-EGFP co-localizes with cellular ACCA protein in the cytoplasm of RAO-3 cells O60218 transfection