IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of tumor cell mitochondrial homeostasis by an organelle-specific hsp90 chaperone network. Regulation of tumor cell mitochondrial homeostasis by an organelle-specific hsp90 chaperone network. Cell (0092-8674) 2007 imex curation Kang BH., Plescia J., Dohi T., Rosa J., Doxsey SJ., Altieri DC. Cancer - Interactions investigated in the context of cancer Fractionation of Raji mitochondrial extracts over Shepherdin-Sepharose was carried out. A unique UniProtKB entry could not be associated with Hsp90 since the exact gene product of interactor could not be ascertained. rarlingh@mdanderson.org DAVET Only protein-protein interactions 1 human-raji Homo sapiens RAJI Burkitt lymphoma cells raji Human Burkitt lymphoma RAJI cells affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Kang BH., Plescia J., Dohi T., Rosa J., Doxsey SJ., Altieri DC. Cancer - Interactions investigated in the context of cancer Fractionation of Raji mitochondrial extracts over Shepherdin-Sepharose was carried out. A unique UniProtKB entry could not be associated with Hsp90 since the exact gene product of interactor could not be ascertained. rarlingh@mdanderson.org Accepted 2007-DEC-23 by DAVET. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 1 imex curation shepherdin_peptide Shepherdin peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KHSSGCAFL An amino acid sequence which is not represented in UniProt Thie sequence of Sheperdin is based on a sequence from survivin. E78ECDDEB865A5B1 trap1_human Heat shock protein 75 kDa, mitochondrial TRAP1 HSP75 Tumor necrosis factor type 1 receptor-associated protein TNFR-associated protein 1 protein protein human Homo sapiens Human MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLRHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFVSFPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH DIP protein Q12931 original sequence version: 72 4B16DE3D2B9E0285 shepherdin-trap1 1 3 unspecified role unspecified role prey prey human-raji Homo sapiens RAJI Burkitt lymphoma cells raji Human Burkitt lymphoma RAJI cells 2 unspecified role unspecified role bait bait physical association physical association Fractionation of Raji mitochondrial extracts over Shepherdin-Sepharose resulted in the specific elution of both TRAP-1 and Hsp90 3 G Trap-1 was not bound by the scrambled peptidomimetic coupled to Sepharose.