IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. Cancer Res. (0008-5472) 2007 imex curation Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer rauscher@wistar.org. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. davet Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells confocal microscopy confocal microscopy immunostaining immunostaining Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer add lex a tag instead of tag to gfi-1 when lex a tag is available rauscher@wistar.org. Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. Cancer Res. (0008-5472) 2007 davet Only protein-protein interactions imex curation The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. Cancer Res. (0008-5472) 2007 imex curation Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer rauscher@wistar.org. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer rauscher@wistar.org. Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. Cancer Res. (0008-5472) 2007 davet Only protein-protein interactions imex curation The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. Cancer Res. (0008-5472) 2007 imex curation Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer rauscher@wistar.org. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer rauscher@wistar.org. Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. Cancer Res. (0008-5472) 2007 davet Only protein-protein interactions imex curation The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. The Ajuba LIM domain protein is a corepressor for SNAG domain mediated repression and participates in nucleocytoplasmic Shuttling. Cancer Res. (0008-5472) 2007 imex curation Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer rauscher@wistar.org. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. davet Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ayyanathan K., Peng H., Hou Z., Fredericks WJ., Goyal RK., Langer EM., Longmore GD., Rauscher FJ. Cancer - Interactions investigated in the context of cancer add lex a tag instead of tag to gfi-1 when lex a tag is available rauscher@wistar.org. Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. A murine embryonic (E9-E11) library was used. Cancer Res. (0008-5472) 2007 davet Only protein-protein interactions imex curation gfi1_human Zinc finger protein Gfi-1 Growth factor independent protein 1 Zinc finger protein 163 GFI1 ZNF163 protein protein human Homo sapiens Human MPRSFLVKSKKAHSYHQPRSPGPDYSLRLENVPAPSRADSTSNAGGAKAEPRDRLSPESQLTEAPDRASASPDSCEGSVCERSSEFEDFWRPPSPSASPASEKSMCPSLDEAQPFPLPFKPYSWSGLAGSDLRHLVQSYRPCGALERGAGLGLFCEPAPEPGHPAALYGPKRAAGGAGAGAPGSCSAGAGATAGPGLGLYGDFGSAAAGLYERPTAAAGLLYPERGHGLHADKGAGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK DIP protein Q99684 original sequence version: 93 DIP protein Q99684 original sequence version: 94 740A466F72C5FC72 limd1_mouse LIM domain-containing protein 1 Limd1 protein protein mouse Mus musculus Mouse MDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQQQQQLLQEEALPRAGRSPVNGGNRQGASGKLAADGAAKPPLAVPTVAPGLATTTAAAQPSYPSQEQRIRPSAHGARPGSQNCGSREGPVSSQRPALHGLSPSCEDPSCLTHGDYYDNFSLASPQWGDKPEGCPSVSLGVGSGWPGCPGNDSTLPKSCGDHHPYQPQLSTVCSGRSFESGISGQDGGIGGHSSEKPTGLWSTASSQRVNLGFSSMGLENGTSAQPKGTTVSAPMVPSSASQGACPKRDSGLGYEASGRVFKPLVDTQPWLQDGPKSYLSVSAPLSSTAGKDSTQPGMTTGLDPKFGCVESGTSPKPSPTSNVHPVMSTPSELSCKESSPSWSTDSSLEPVLPGSPTPSRVRLPCQTLAPGPELGPSTAELKLEALTQRLEREMDAHPKADYFGSCVKCSKGVFGAGQACQAMGDLYHDACFTCAACSRKLRGKAFYFVNGKVFCEEDFLYSGFQQSADRCFLCGHLIMDMILQALGKSYHPGCFRCVICNECLDGVPFTVDSENKIYCVRDYHKVLAPKCAACGLPILPPEGSDETIRVVSMDRDYHVECYHCEDCGLELNDEDGHRCYPLEDHLFCHSCHVKRLEKGPSPAPLHQHHF DIP protein Q9QXD8 original sequence version: 85 68C05A7BADEBDC92 ajuba_mouse LIM domain-containing protein ajuba Ajuba Jub protein protein mouse Mus musculus Mouse MERLGEKASRLLEKLRLSDSGSAKFGRRKGEASRSGSDGTPGAGKGRLSGLGGPRKSGHRGANGGPGDEPLEPAREQGPLDAERNARGSFEAQRFEGSFPGGPPPTRALPLPLSSPPDFRLETTAPALSPRSSFASSSASDASKPSSPRGSLLLDGAGASGAGGSRPCSNRTSGISMGYDQRHGSPLPAGPCLFGLPLTTAPAGYPGGAPSAYPELHAALDRLCAHRSVGFGCQESRHSYPPALGSPGALTGAVVGTAGPLERRGAQPGRHSVTGYGDCAAGARYQDELTALLRLTVATGGREAGARGEPSGIEPSGLEESPGPFVPEASRSRIREPEAREDYFGTCIKCNKGIYGQSNACQALDSLYHTQCFVCCSCGRTLRCKAFYSVNGSVYCEEDYLFSGFQEAAEKCCVCGHLILEKILQAMGKSYHPGCFRCIVCNKCLDGVPFTVDFSNQVYCVTDYHKNYAPKCAACGQPILPSEGCEDIVRVISMDRDYHFECYHCEDCRMQLSDEEGCCCFPLDGHLLCHGCHMQRLSARQPSTNYI DIP protein Q91XC0 original sequence version: 32 DIP protein Q91XC0 original sequence version: 65 C326772AC1C155B2 gfi1-jub 2 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association gfi1-limd1 2 5 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association limd1-gfi1 3 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99684:p.[Phe5Ala;Leu6Ala;Val7Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain V A Wed May 02 17:58:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala,leu6ala,val7ala' Tue Jun 30 18:58:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe5Ala;Leu6Ala;Val7Ala]' Q99684:p.[Lys10Ala;Lys11Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:56:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys10ala,lys11ala' Tue Jun 30 20:16:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys10Ala;Lys11Ala]' Q99684:p.Phe5Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:15:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala' Tue Jun 30 17:52:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe5Ala' physical association physical association 2 B, C jub-gfi1 3 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99684:p.[Phe5Ala;Leu6Ala;Val7Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain V A Wed May 02 15:13:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala,leu6ala,val7ala' Tue Jun 30 17:32:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe5Ala;Leu6Ala;Val7Ala]' Q99684:p.[Lys10Ala;Lys11Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:00:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys10ala,lys11ala' Tue Jun 30 18:56:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys10Ala;Lys11Ala]' Q99684:p.Phe5Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:58:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala' Tue Jun 30 17:44:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe5Ala' physical association physical association 2 B, C gfi1-jub-2 4 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait Q99684:p.[Lys10Ala;Lys11Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:33:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys10ala,lys11ala' Tue Jun 30 18:08:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys10Ala;Lys11Ala]' region LEXA tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q99684:p.[Phe5Ala;Leu6Ala;Val7Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain V A Wed May 02 17:17:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala,leu6ala,val7ala' Tue Jun 30 18:53:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe5Ala;Leu6Ala;Val7Ala]' Q99684:p.Phe5Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:09:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala' Tue Jun 30 16:50:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe5Ala' n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3 A gfi1-jub-1 1 7 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99684:p.[Phe5Ala;Leu6Ala;Val7Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain V A Wed May 02 18:31:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala,leu6ala,val7ala' Tue Jun 30 17:12:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe5Ala;Leu6Ala;Val7Ala]' Q99684:p.Phe5Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:53:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe5ala' Tue Jun 30 17:43:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe5Ala' region LEXA tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 4 B