IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer Details for Pull Down method obtained from Shen et al,. Genes + Dev. 1998. GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat. Struct. Mol. Biol. (1545-9993) 2007 imex curation Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer Rather odd protocol for this EMSA- both the bait AND prey are radio-labelled with beta emitters.... (also the Supplement method gives TWO antibodies....??) GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Accepted 2012-JUN-06 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 bax_human_gene Human BAX gene gene gene human Homo sapiens Human ep300_human Histone acetyltransferase p300 Histone butyryltransferase p300 Histone crotonyltransferase p300 Protein propionyltransferase p300 EP300 P300 E1A-associated protein p300 Protein 2-hydroxyisobutyryltransferase p300 Protein lactyltransferas p300 protein protein human Homo sapiens Human MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEPLQQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLSNNLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQIDPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVNGGVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMIRGSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGPRMQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQGSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPPGPQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAPLLPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDDESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLRRRMASMQRTGVVGQQQGLPSPTPATPTTPTGQQPTTPQTPQPTSQPQPTPPNSMPPYLPRTQAAGPVSQGKAAGQVTPPTPPQTAQPPLPGPPPAAVEMAMQIQRAAETQRQMAHVQIFQRPIQHQMPPMTPMAPMGMNPPPMTRGPSGHLEPGMGPTGMQQQPPWSQGGLPQPQQLQSGMPRPAMMSVAQHGQPLNMAPQPGLGQVGISPLKPGTVSQQALQNLLRTLRSPSSPLQQQQVLSILHANPQLLAAFIKQRAAKYANSNPQPIPGQPGMPQGQPGLQPPTMPGQQGVHSNPAMQNMNPMQAGVQRAGLPQQQPQQQLQPPMGGMSPQAQQMNMNHNTMPSQFRDILRRQQMMQQQQQQGAGPGIGPGMANHNQFQQPQGVGYPPQQQQRMQHHMQQMQQGNMGQIGQLPQALGAEAGASLQAYQQRLLQQQMGSPVQPNPMSPQQHMLPNQAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPSPRMQPQPSPHHVSPQTSSPHPGLVAAQANPMEQGHFASPDQNSMLSQLASNPGMANLHGASATDLGLSTDNSDLNSNLSQSTLDIH DIP protein Q09472 original sequence version: 193 DIP protein Q09472 original sequence version: 186 DIP protein Q09472 original sequence version: 107 DIP protein Q09472 original sequence version: 112 DIP protein Q09472 original sequence version: 181 DIP protein Q09472 original sequence version: 183 DIP protein Q09472 original sequence version: 136 DIP protein Q09472 original sequence version: 146 DIP protein Q09472 original sequence version: 165 DIP protein Q09472 original sequence version: 192 DIP protein Q09472 original sequence version: 200 DIP protein Q09472 original sequence version: 206 DIP protein Q09472 original sequence version: 194 DIP protein Q09472 original sequence version: 171 DIP protein Q09472 original sequence version: 142 DIP protein Q09472 original sequence version: 180 8E869E1F174A6FEB iaspp_human RelA-associated inhibitor PPP1R13L IASPP NKIP1 PPP1R13BL RAI Inhibitor of ASPP protein NFkB-interacting protein 1 PPP1R13B-like protein protein protein human Homo sapiens Human MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQPQPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV DIP protein Q8WUF5 original sequence version: 129 DIP protein Q8WUF5 original sequence version: 52 7BEEF239B7CA9C70 probe_p21_(cdkn1a)_p53_dna_binding_site CDKN1A_ p53 binding site dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) AGCTTAGGCATGTCTAGGCATGTCTA Blatting the stated sequence does not give a "hit" to any genomic region. pin1_human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 PIN1 protein protein human Homo sapiens Human MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE DIP protein Q13526 original sequence version: 65 DIP protein Q13526 original sequence version: 64 DIP protein Q13526 original sequence version: 163 DIP protein Q13526 original sequence version: 156 DIP protein Q13526 original sequence version: 117 35391AF40B7D1E13 cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human tp53-cdkn1a-9 7 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role ancillary ancillary 9 unspecified role unspecified role bait bait association association Fig IB, 1C, Fig 2. UV light, etoposide, Nutlin-3a (inhibits Mdm2 binding to p53). Inhibition of Pin1 expression in stressed cells, reduced the binding of p53 to its upstream responsive site (BS1) on the p21 promoter. tp53-bax-1 7 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role ancillary ancillary association association Fig IC, fig 2. UV light, etoposide, Nutlin-3A (inhibits Mdm2 binding to p53). RNAi inhibition of Pin1 expression in stressed cells, and stabilizing p53 with nutlin-3a, show that Pin1 is required for optimal binding of p53 to both p21 and BAX promoters. pin1-cdkn1a 7 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait association association Fig 2, S1B. etoposide. DNA damage–induced recruitment of Pin1 is only slightly evident in HCT116 p53-/- cells. (Fig S1B) pin1-bax-1 7 10 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait association association Fig 2, S1B. etoposide. DNA damage–induced recruitment of Pin1 is only slightly evident in HCT116 p53-/- cells. (Fig S1B). pin1-tp53-14 6 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 11:10:13 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser46ala,thr81ala,ser315ala,ser33ala' Wed May 02 19:04:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser315ala' Tue Jun 30 20:21:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala]' P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 11:10:18 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr81ala,ser315ala,ser46ala,ser127ala,thr150ala,ser33ala' Wed May 02 17:23:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser127ala,thr150ala,ser315ala' Tue Jun 30 20:11:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala]' human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma physical association physical association Fig 3A pin1-cdkn1a-3 5 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role ancillary ancillary P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 11:00:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr81ala,ser315ala,ser46ala,ser127ala,thr150ala,ser33ala' Wed May 02 14:37:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser127ala,thr150ala,ser315ala' Tue Jun 30 20:20:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala]' P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 10:49:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser46ala,thr81ala,ser315ala,ser33ala' Wed May 02 16:45:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser315ala' Tue Jun 30 18:57:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala]' 14 unspecified role unspecified role bait bait association association Fig 3B tp53-cdkn1a-10 3 9 unspecified role unspecified role bait bait P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 11:04:47 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser46ala,thr81ala,ser315ala,ser33ala' Wed May 02 18:17:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser315ala' Tue Jun 30 16:52:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala]' 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 3D etoposide ep300-tp53-2 4 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig 4C RNAi-mediated inhibition of Pin1 expression in HCT116 cells reduced the interaction of endogenous p53 and p300 proteins. tp53-cdkn1a-11 5 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait P04637:p.[Lys319Arg;Lys320Arg;Lys321Arg;Lys370Arg;Lys372Arg;Lys373Arg;Lys381Arg;Lys382Arg;Lys386Arg] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R Wed May 02 20:27:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys319arg,lys320arg,lys321arg,lys370arg,lys372arg,lys373arg,lys381arg,lys382arg,lys386arg' Tue Jun 30 15:50:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys319Arg;Lys320Arg;Lys321Arg;Lys370Arg;Lys372Arg;Lys373Arg;Lys381Arg;Lys382Arg;Lys386Arg]' association association Fig 5A/B p53-9KR showed reduced binding to p21 promoter as compared to WT p53 expressed at similar levels. A similar affect was observed in RNAi-mediated inhibition of Pin1. tp53-cdkn1a-12 7 9 unspecified role unspecified role bait bait ac-lys373/382 n6aclys N6-acetyl-L-lysine (2S)-6-acetamido-2-aminohexanoic acid 6-acetylamino-2-aminohexanoic acid 6-acetylazanyl-2-aminohexanoic acid MOD_RES N6-acetyllysine N(zeta)-acetyllysine N6-acetyl-L-lysine N6-acetylated L-lysine epsilon-acetyllysine Acetyl Acetylation N6AcLys acetylk certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 5C Acetylation of promoter-bound p53 on Lys373 and Lys382 increased upon stress only in the presence of normal levels of Pin1, and could be blocked by inhibition of Pin1's catalytic activity with the small molecule PiB. PiB. ep300-cdkn1a-1 7 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association 5D RNAi for Pin1. tp53-cdkn1a-13 3 9 unspecified role unspecified role bait bait P04637:p.Thr81Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Wed May 02 20:22:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr81ala' Tue Jun 30 17:34:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr81Ala' lys382 n6aclys N6-acetyl-L-lysine (2S)-6-acetamido-2-aminohexanoic acid 6-acetylamino-2-aminohexanoic acid 6-acetylazanyl-2-aminohexanoic acid MOD_RES N6-acetyllysine N(zeta)-acetyllysine N6-acetyl-L-lysine N6-acetylated L-lysine epsilon-acetyllysine Acetyl Acetylation N6AcLys acetylk certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 6B etoposide, UV. ppp1r13l-tp53-2 2 9 unspecified role unspecified role prey prey P04637:p.Ser46Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:26:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser46ala' Tue Jun 30 18:09:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser46Ala' 12 unspecified role unspecified role bait bait physical association physical association Fig 7A/C/D, S8A. cytotoxic UV dosage Overexpression of catalytically active Pin1 enhanced p53 dissociation from iASPP. Disruption of single Pin1 target sites on p53 demonstrated that stress-induced dissociation of p53 from iASPP required Ser46 and, to a lesser extent, Thr81. I have not added Thr81 as a mutation as results looks hardly any different - to me- than WT, and the text does not indicate anything different, imo. ppp1r13l-tp53-1 4 12 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig 7B, S7B, S8A. cytotoxic UV dosage In the absence of Pin1, the dissociation of p53 from iASPP upon cytotoxic stress was impaired. The Pin1 inhibitor PiB partially reversed the Etoposide induced dissociation of p53 from iASPP. Pin1 inhibitor - PiB tp53-cdkn1a-14 1 15 unspecified role unspecified role prey prey bs1_upstream_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait association association Fig S5B etoposide. pin1-cdkn1a-4 1 14 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey bs1+2_promoter_regions binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association S1A. UV light, etoposide. pin1-tp53-15 6 9 unspecified role unspecified role prey prey P04637:p.Pro72Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain P R Wed May 02 16:41:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro72arg' Tue Jun 30 20:06:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro72Arg' P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 10:51:17 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr81ala,ser315ala,ser46ala,ser127ala,thr150ala,ser33ala' Wed May 02 16:16:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser127ala,thr150ala,ser315ala' Tue Jun 30 17:45:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala]' human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig S2B, S5A, S6C. lambda phosphatase Binding was observed using mutant p53 proteins with only one intact Pin1-consensus site. p53-9KR, (a mutant where nine C-terminal lysines, including those acetylated by p300, are substituted by arginines), bound Pin1 to a similar extent as did WT p53. p21-tp53 8 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P04637:p.Arg175His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H Wed May 02 15:05:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 15:53:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 10:55:53 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr81ala,ser315ala,ser46ala,ser127ala,thr150ala,ser33ala' Wed May 02 16:21:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser127ala,thr150ala,ser315ala' Tue Jun 30 16:13:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala]' rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 13 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig S3A lambda phosphatase Binding was observed using mutant p53 proteins with only one intact Pin1-consensus site. tp53-cdkn1a-15 1 9 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey bs1+2_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S1A UV light, etoposide. tp53-cdkn1a-16 1 9 unspecified role unspecified role bait bait ac-lys382 n6aclys N6-acetyl-L-lysine (2S)-6-acetamido-2-aminohexanoic acid 6-acetylamino-2-aminohexanoic acid 6-acetylazanyl-2-aminohexanoic acid MOD_RES N6-acetyllysine N(zeta)-acetyllysine N6-acetyl-L-lysine N6-acetylated L-lysine epsilon-acetyllysine Acetyl Acetylation N6AcLys acetylk certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey bs1_upstream_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S5B etoposide. ep300-cdkn1a 1 11 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey bs1_upstream_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S5B etoposide. Binding was reduced when Pin1 was knocked down. iaspp-tp53-4 2 12 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 11:00:26 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser46ala,thr81ala,ser315ala,ser127ala,thr150ala,ser33ala' Wed May 02 14:53:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser127ala,thr150ala,ser315ala' Tue Jun 30 15:54:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala]' P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 11:14:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser46ala,thr81ala,ser315ala,ser33ala' Wed May 02 14:51:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser33ala,ser46ala,thr81ala,ser315ala' Tue Jun 30 18:36:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala]' physical association physical association Fig S7A