IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Expression, purification, and characterization of Tara, a novel telomere repeat-binding factor 1 (TRF1)-binding protein. Expression, purification, and characterization of Tara, a novel telomere repeat-binding factor 1 (TRF1)-binding protein. Protein Expr. Purif. (1046-5928) 2007 imex curation Li X., Lan J., Zhu Y., Yu J., Dou Z., Huang H. Cancer - Interactions investigated in the context of cancer The recombinant TRF1 protein is indicated to be of human orgin by reference 4 and the statement in the introduction that human telomere functions require TRF1. The authors specify that the isoform of Tara corresponds to NP_008963.2. Using Align it was found that this sequence corresponds to Q9H2D6-5. hehuang.zju@gmail.com 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Li X., Lan J., Zhu Y., Yu J., Dou Z., Huang H. Cancer - Interactions investigated in the context of cancer The recombinant TRF1 protein is indicated to be of human orgin by reference 4 and the statement in the introduction that human telomere functions require TRF1. The authors specify that the isoform of Tara corresponds to NP_008963.2. Using Align it was found that this sequence corresponds to Q9H2D6-5. hehuang.zju@gmail.com Accepted 2007-NOV-17 by DAVET. Protein Expr. Purif. (1046-5928) 2007 davet Only protein-protein interactions imex curation Expression, purification, and characterization of Tara, a novel telomere repeat-binding factor 1 (TRF1)-binding protein. Expression, purification, and characterization of Tara, a novel telomere repeat-binding factor 1 (TRF1)-binding protein. Protein Expr. Purif. (1046-5928) 2007 imex curation Li X., Lan J., Zhu Y., Yu J., Dou Z., Huang H. Cancer - Interactions investigated in the context of cancer The recombinant TRF1 protein is indicated to be of human orgin by reference 4 and the statement in the introduction that human telomere functions require TRF1. The authors specify that the isoform of Tara corresponds to NP_008963.2. Using Align it was found that this sequence corresponds to Q9H2D6-5. hehuang.zju@gmail.com 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li X., Lan J., Zhu Y., Yu J., Dou Z., Huang H. Cancer - Interactions investigated in the context of cancer The recombinant TRF1 protein is indicated to be of human orgin by reference 4 and the statement in the introduction that human telomere functions require TRF1. The authors specify that the isoform of Tara corresponds to NP_008963.2. Using Align it was found that this sequence corresponds to Q9H2D6-5. hehuang.zju@gmail.com Accepted 2007-NOV-17 by DAVET. 13-Nov-2007: Contacted by JYOTI. Protein Expr. Purif. (1046-5928) 2007 davet Only protein-protein interactions imex curation q9h2d6-5 TRIO and F-actin-binding protein TRIOBP-1 TRIOBP KIAA1662 TARA Protein Tara Trio-associated repeat on actin HRIHFB2122 protein protein human Homo sapiens Human MTPDLLNFKKGWMSILDEPGEPPSPSLTTTSTSQWKKHWFVLTDSSLKYYRDSTAEEADELDGEIDLRSCTDVTEYAVQRNYGFQIHTKDAVYTLSAMTSGIRRNWIEALRKTVRPTSAPDVTKLSDSNKENALHSYSTQKGPLKAGEQRAGSEVISRGGPRKADGQRQALDYVELSPLTQASPQRARTPARTPDRLAKQEELERDLAQRSEERRKWFEATDSRTPEVPAGEGPRRGLGAPLTEDQQNRLSEEIEKKWQELEKLPLRENKRVPLTALLNQSRGERRGPPSDGHEALEKEVQALRAQLEAWRLQGEAPQSALRSQEDGHIPPGYISQEACERSLAEMESSHQQVMEELQRHHERELQRLQQEKEWLLAEETAATASAIEAMKKAYQEELSRELSKTRSLQQGPDGLRKQHQSDVEALKRELQVLSEQYSQKCLEIGALMRQAEEREHTLRRCQQEGQELLRHNQELHGRLSEEIDQLRGFIASQGMGNGCGRSNERSSCELEVLLRVKENELQYLKKEVQCLRDELQMMQKDKRFTSGKYQDVYVELSHIKTRSEREIEQLKEHLRLAMAALQEKESMRNSLAE Contains a phosphothreonine at position 457. 109AA7D9CC10B21A terf1_human Telomeric repeat-binding factor 1 TTAGGG repeat-binding factor 1 NIMA-interacting protein 2 Telomeric protein Pin2/TRF1 TERF1 PIN2 TRF TRF1 TRBF1 protein protein human Homo sapiens Human MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED DIP protein P54274 original sequence version: 121 DIP protein P54274-1 original sequence version: 81 DIP protein P54274 original sequence version: 168 AB548E7D3124A211 triobp-triobp 1 3 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) physical association physical association 3 Using a mixture of proteins with known molecular weights as calibration markers for the gel-filtration column, we estimated the molecular weight of the protein eluted in fraction 50 be 480 kDa. Since Tara can form a dimer via its coiled-coil domain [1], the result suggested that His-Tara existed as an octamer under the described buffer condition. MW = 480 kDa (estimated) Since the estimated MW is 480 kDa and Tara is known to be able to form a dimer via its coiled-coil domain, the result suggests that His-Tara exists as an octamer under these conditions. Q9H2D6-5 protein NP_008963.2 terf1-triobp 2 3 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 7 B With GST-TRF1 as the bait protein, Tara was recovered in the prey complex PREY protein NP_008963.2