IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. A HeLa cell cDNA library (Clontech) was used. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression. Mol. Cell. Biochem. (0300-8177) 2007 imex curation Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer oncology@sdu.edu.cn A HeLa cell cDNA library (Clontech) was used. DAVET Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gao H., Yu Z., Bi D., Jiang L., Cui Y., Sun J., Ma R. Chromatin - Epigenetic interactions resulting in chromatin modulation oncology@sdu.edu.cn Accepted 2007-DEC-23 by DAVET. Mol. Cell. Biochem. (0300-8177) 2007 DAVET Only protein-protein interactions imex curation akt2_human RAC-beta serine/threonine-protein kinase AKT2 RAC-PK-beta Protein kinase Akt-2 Protein kinase B beta protein protein human Homo sapiens Human MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWMRAIQMVANSLKQRAPGEDPMDYKCGSPSDSSTTEEMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLSPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIRE DIP protein P31751 original sequence version: 137 DIP protein P31751 original sequence version: 155 B18C87A7246BFB24 setb1_human Histone-lysine N-methyltransferase SETDB1 SETDB1 KIAA0067 KMT1E SET domain bifurcated 1 ERG-associated protein with SET domain Histone H3-K9 methyltransferase 4 Lysine N-methyltransferase 1E ESET protein protein human Homo sapiens Human MSSLPGCIGLDAATATVESEEIAELQQAVVEELGISMEELRHFIDEELEKMDCVQQRKKQLAELETWVIQKESEVAHVDQLFDDASRAVTNCESLVKDFYSKLGLQYRDSSSEDESSRPTEIIEIPDEDDDVLSIDSGDAGSRTPKDQKLREAMAALRKSAQDVQKFMDAVNKKSSSQDLHKGTLSQMSGELSKDGDLIVSMRILGKKRTKTWHKGTLIAIQTVGPGKKYKVKFDNKGKSLLSGNHIAYDYHPPADKLYVGSRVVAKYKDGNQVWLYAGIVAETPNVKNKLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDISCRDFIEEYVTAYPNRPMVLLKSGQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIYRGSTRLEPMFSMKTSSASALEKKQGQLRTRPNMGAVRSKGPVVQYTQDLTGTGTQFKPVEPPQPTAPPAPPFPPAPPLSPQAGDSDLESQLAQSRKQVAKKSTSFRPGSVGSGHSSPTSPALSENVSGGKPGINQTYRSPLGSTASAPAPSALPAPPAPPVFHGMLERAPAEPSYRAPMEKLFYLPHVCSYTCLSRVRPMRNEQYRGKNPLLVPLLYDFRRMTARRRVNRKMGFHVIYKTPCGLCLRTMQEIERYLFETGCDFLFLEMFCLDPYVLVDRKFQPYKPFYYILDITYGKEDVPLSCVNEIDTTPPPQVAYSKERIPGKGVFINTGPEFLVGCDCKDGCRDKSKCACHQLTIQATACTPGGQINPNSGYQYKRLEECLPTGVYECNKRCKCDPNMCTNRLVQHGLQVRLQLFKTQNKGWGIRCLDDIAKGSFVCIYAGKILTDDFADKEGLEMGDEYFANLDHIESVENFKEGYESDAPCSSDSSGVDLKDQEDGNSGTEDPEESNDDSSDDNFCKDEDFSTSSVWRSYATRRQTRGQKENGLSETTSKDSHPPDLGPPHIPVPPSIPVGGCNPPSSEETPKNKVASWLSCNSVSEGGFADSDSHSSFKTNEGGEGRAGGSRMEAEKASTSGLGIKDEGDIKQAKKEDTDDRNKMSVVTESSRNYGYNPSPVKPEGLRRPPSKTSMHQSRRLMASAQSNPDDVLTLSSSTESEGESGTSRKPTAGQTSATAVDSDDIQTISSGSEGDDFEDKKNMTGPMKRQVAVKSTRGFALKSTHGIAIKSTNMASVDKGESAPVRKNTRQFYDGEESCYIIDAKLEGNLGRYLNHSCSPNLFVQNVFVDTHDLRFPWVAFFASKRIRAGTELTWDYNYEVGSVEGKELLCCCGAIECRGRLL DIP protein Q15047 original sequence version: 167 DIP protein Q15047 original sequence version: 124 D8841B4C41B911C5 akt1_human RAC-alpha serine/threonine-protein kinase AKT1 PKB RAC RAC-PK-alpha Protein kinase B Proto-oncogene c-Akt Protein kinase B alpha protein protein human Homo sapiens Human MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA DIP protein P31749 original sequence version: 184 DIP protein P31749 original sequence version: 168 DIP protein P31749 original sequence version: 102 DIP protein P31749 original sequence version: 110 DIP protein P31749 original sequence version: 160 DIP protein P31749 original sequence version: 136 DIP protein P31749 original sequence version: 175 DIP protein P31749 original sequence version: 201 DIP protein P31749 original sequence version: 166 DIP protein P31749 original sequence version: 178 DIP protein P31749-1 original sequence version: 194 DIP protein P31749 original sequence version: 106 DIP protein P31749 original sequence version: 141 6EAFF4F8AD436714 akt1-setdb1 3 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Full-length human Akt1 was used as bait for screening in a HeLa cDNA library. In this library, cDNAs are fused to the GAL4 activation domain in pGADT7. Of 106 transformants, 30 conferred to pGBKT7- Akt1-baits cells the ability to grow on His-plates and to produce b-galactosidase. Of these, 20 were true positives that could not induce His- or b-galactosidase production when transformed into Y190/pGBKT7 cells. One clone encoding the N-terminal amino acids (aa) 221-546 of human SETDB1 was isolated akt1-setdb1-1 4 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 1 B yeast cells expressing Akt1 together with SETDB1 could grow on both plates, suggesting the true interaction between Akt1 and SETDB1 akt1-setdb1-2 7 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 1 C GSTAkt1-bound SETDB1 proteins were detected by autoradiography. setdb1-akt1 1 11 unspecified role unspecified role prey prey P31749:p.Glu40Lys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K Wed May 02 14:42:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu40lys' Tue Jun 30 19:56:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu40Lys' P31749:p.Lys179Met mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:56:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys179met' Tue Jun 30 19:57:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys179Met' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 A wild type (wt) Akt1 proteins could be co-immunoprecipitated by SETDB1 Q15047; P31749 transfection setdb1-akt1-1 5 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 2 B endogenous Akt1 protein could be co-immunoprecipitated with endogenous SETDB1 proteins akt1-setdb1-3 8 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2 C Myc-SETDB1 and GST-Akt1 or GST were co-expressed in HEK293T cells and GST-fused proteins were purified with glutathione sepharose 4B. As shown in Fig. 2C, SETDB1 could be copurified with GST-Akt1 but not GST alone. akt1-setdb1-4 2 10 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3 A In HeLa cells, overexpressed Akt1 was localized both in the cytoplasm and the nucleus (Fig. 3A, left panel), whereas SETDB1 was absolutely in the nucleus (middle panel). Akt1 and SETDB1 were co-resided in the nucleus P31749; Q15047 transfection P31749 overexpressed akt1-setdb1-5 6 10 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3 B Akt1 and SETDB1 were co-resided in the nucleus (Fig. 3A, right panel, yellow). The result also implied that a proportion of Akt1 proteins were recruited or retained in the nucleus, since Akt1 alone is mainly in the cytoplasm. Similar results were also observed in mouse fibroblast NIH3T3 cells P31749; Q15047 transfection akt2-setdb1 1 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4 Co-IP assays showed that SETDB1 interaction with both Akt1 and Akt2 (Fig. 4). Interestingly, it seems that the affinity of SETDB1 with Akt2 was higher than SETDB1 with Akt1, due to the fact that more SETDB1 proteins were co-immunoprecipitated by Akt2 (Fig. 4, top panel, lane 8) when equal amounts of Akt1 and Akt2 proteins were immunoprecipitated by Flag antibodies More SETDB1 was coimmunoprecipitated by Akt2 than Akt1 when equal amounts of Akt1 and Akt2 proteins were immunoprecipitated by Flag antibodies, indicating that the affinity of SETDB1 for Akt2 was higher than for Akt1. akt1-setdb1-6 1 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4 Co-IP assays showed that SETDB1 interaction with both Akt1 and Akt2