IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A yeast two-hybrid system using Sp17 identified Ropporin as a novel cancer-testis antigen in hematologic malignancies. A yeast two-hybrid system using Sp17 identified Ropporin as a novel cancer-testis antigen in hematologic malignancies. Int. J. Cancer (0020-7136) 2007 imex curation Li Z., Li W., Meklat F., Wang Z., Zhang J., Zhang Y., Lim SH. Cancer - Interactions investigated in the context of cancer The authors confirmed by email that the SP17 construct used was of human origin and that the form of ropporin was ropporin-1. seah.lim@usoncology.com 07-Sept-2007: Contacted by JYOTI. A human testis cDNA library was used. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li Z., Li W., Meklat F., Wang Z., Zhang J., Zhang Y., Lim SH. Cancer - Interactions investigated in the context of cancer The authors confirmed by email that the SP17 construct used was of human origin and that the form of ropporin was ropporin-1. seah.lim@usoncology.com Accepted 2007-SEP-16 by JYOTI. 07-Sept-2007: Contacted by JYOTI. A human testis cDNA library was used. Int. J. Cancer (0020-7136) 2007 Only protein-protein interactions imex curation rop1a_human Ropporin-1A ROPN1A Rhophilin-associated protein 1A Cancer/testis antigen 91 ROPN1 protein protein human Homo sapiens Human MAQTDKPTCIPPELPKMLKEFAKAAIRVQPQDLIQWAADYFEALSRGETPPVRERSERVALCNRAELTPELLKILHSQVAGRLIIRAEELAQMWKVVNLPTDLFNSVMNVGRFTEEIEWLKFLALACSALGVTITKTLKIVCEVLSCDHNGGSPRIPFSTFQFLYTYIAKVDGEISASHVSRMLNYMEQEVIGPDGIITVNDFTQNPRVQLE B50D2859B464F6CC sp17_human Sperm surface protein Sp17 SPA17 SP17 Sperm autoantigenic protein 17 Sp17-1 Cancer/testis antigen 22 protein protein human Homo sapiens Human MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAKKMKTNSLQNEEKEENK BC99EC310FA0E54A spa17-ropn1 1 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Applying Sperm protein 17 (Sp17) as the bait in a yeast 2-hybrid system of a testicular cDNA library, 17 interacting clones were isolated and all encoded Ropporin, a spermatogenic cell-specific protein that serves as an anchoring protein for the A-kinase anchoring protein, AKAP110.