IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. Cancer Cell (1535-6108) 2007 imex curation Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions 20 human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Rejected 2007-MAY-25 by DAVET. I think the same comments made in gordan-2007-1 are relevant here also. Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. Cancer Cell (1535-6108) 2007 imex curation Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions 20 human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. Cancer Cell (1535-6108) 2007 imex curation Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions 20 human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. Cancer Cell (1535-6108) 2007 imex curation Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions 20 human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. Cancer Cell (1535-6108) 2007 imex curation Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions 20 human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. Cancer Cell (1535-6108) 2007 imex curation Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions 20 human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation cdn1a_human Cyclin-dependent kinase inhibitor 1 CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 protein protein human Homo sapiens Human MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP DIP protein P38936 original sequence version: 120 DIP protein P38936 original sequence version: 165 DIP protein P38936 original sequence version: 160 98D1E7C519ADFCA9 max_human Protein max MAX BHLHD4 Class D basic helix-loop-helix protein 4 Myc-associated factor X protein protein human Homo sapiens Human MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS DIP protein P61244 original sequence version: 132 DIP protein P61244 original sequence version: 128 DIP protein P61244 original sequence version: 150 DIP protein P61244 original sequence version: 120 EB10F3137727A56F myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA DIP protein P01106 original sequence version: 195 DIP protein P01106 original sequence version: 196 DIP protein P01106 original sequence version: 148 DIP protein P01106-1 original sequence version: 161 DIP protein P01106 original sequence version: 182 DIP protein P01106 original sequence version: 197 DIP protein P01106-1 original sequence version: 208 DIP protein P01106 original sequence version: 121 DIP protein P01106-1 original sequence version: 144 DIP protein P01106 original sequence version: 144 ED5C028029A4C5D1 pias2_human E3 SUMO-protein ligase PIAS2 Protein inhibitor of activated STAT x Msx-interacting zinc finger protein DAB2-interacting protein Androgen receptor-interacting protein 3 PIAS-NY protein PIAS2 Protein inhibitor of activated STAT2 PIASX E3 SUMO-protein transferase PIAS2 protein protein human Homo sapiens Human MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIHSLPSTSVTPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPISSMSSDLPGLDFLSLIPVDPQYCPPMFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD 6422B383345AD883 cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT DIP protein P46527 original sequence version: 120 DIP protein P46527 original sequence version: 115 DIP protein P46527 original sequence version: 154 DIP protein P46527 original sequence version: 168 DIP protein P46527 original sequence version: 164 DIP protein P46527 original sequence version: 118 1118D58901CDF3FC cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P24941 original sequence version: 142 DIP protein P24941 original sequence version: 118 DIP protein P24941 original sequence version: 143 DIP protein P24941 original sequence version: 144 DIP protein P24941 original sequence version: 152 DIP protein P24941 original sequence version: 182 DIP protein P24941-1 original sequence version: 204 DIP protein P24941 original sequence version: 90 DIP protein P24941 original sequence version: 190 DIP protein P24941 original sequence version: 168 F90A0F4E70910B51 hif1a_human Hypoxia-inducible factor 1-alpha ARNT-interacting protein Member of PAS protein 1 Basic-helix-loop-helix-PAS protein MOP1 HIF1A MOP1 BHLHE78 PASD8 PAS domain-containing protein 8 Class E basic helix-loop-helix protein 78 protein protein human Homo sapiens Human MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN DIP protein Q16665 original sequence version: 94 DIP protein Q16665 original sequence version: 98 DIP protein Q16665-1 original sequence version: 102 DIP protein Q16665 original sequence version: 102 DIP protein Q16665 original sequence version: 127 DIP protein Q16665 original sequence version: 120 DIP protein Q16665 original sequence version: 169 DIP protein Q16665 original sequence version: 174 DIP protein Q16665-1 original sequence version: 174 DIP protein Q16665-1 original sequence version: 191 DIP protein Q16665 original sequence version: 191 DIP protein Q16665 original sequence version: 179 DIP protein Q16665 original sequence version: 150 DIP protein Q16665 original sequence version: 156 DIP protein Q16665 original sequence version: 177 ABD4F7DAA135BE2D mad1_human Max dimerization protein 1 MAD Protein MAD MXD1 protein protein human Homo sapiens Human MAAAVRMNIQMLLEAADYLERREREAEHGYASMLPYNNKDRDALKRRNKSKKNNSSSRSTHNEMEKNRRAHLRLCLEKLKGLVPLGPESSRHTTLSLLTKAKLHIKKLEDCDRKAVHQIDQLQREQRHLKRQLEKLGIERIRMDSIGSTVSSERSDSDREEIDVDVESTDYLTGDLDWSSSSVSDSDERGSMQSLGSDEGYSSTSIKRIKLQDSHKACLGL DIP protein Q05195 original sequence version: 72 B39FAEBFD708B6AB sp1_human Transcription factor Sp1 SP1 TSFP1 protein protein human Homo sapiens Human MSDQDHSMDEMTAVVKIEKGVGGNNGGNGNGGGAFSQARSSSTGSSSSTGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTTISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSSGTNSQGQTPQRVSGLQGSDALNIQQNQTSGGSLQAGQQKEGEQNQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCTWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF DIP protein P08047 original sequence version: 185 DIP protein P08047 original sequence version: 187 DIP protein P08047 original sequence version: 181 DIP protein P08047 original sequence version: 162 DIP protein P08047 original sequence version: 161 43893DBF6518B9EA epas1_human Endothelial PAS domain-containing protein 1 EPAS1 HIF2A MOP2 Member of PAS protein 2 Basic-helix-loop-helix-PAS protein MOP2 BHLHE73 PASD2 Hypoxia-inducible factor 2-alpha HIF-1-alpha-like factor PAS domain-containing protein 2 Class E basic helix-loop-helix protein 73 protein protein human Homo sapiens Human MTADKEKKRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSGKGAVSEKSNFLFTKLKEEPEELAQLAPTPGDAIISLDFGNQNFEESSAYGKAILPPSQPWATELRSHSTQSEAGSLPAFTVPQAAAPGSTTPSATSSSSSCSTPNSPEDYYTSLDNDLKIEVIEKLFAMDTEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEERLLAENPQSTPQHCFSAMTNIFQPLAPVAPHSPFLLDKFQQQLESKKTEPEHRPMSSIFFDAGSKASLPPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWAVGDQRTEFLGAAPLGPPVSPPHVSTFKTRSAKGFGARGPDVLSPAMVALSNKLKLKRQLEYEEQAFQDLSGGDPPGGSTSHLMWKRMKNLRGGSCPLMPDKPLSANVPNDKFTQNPMRGLGHPLRHLPLPQPPSAISPGENSKSRFPPQCYATQYQDYSLSSAHKVSGMASRLLGPSFESYLLPELTRYDCEVNVPVLGSSTLLQGGDLLRALDQAT DIP protein Q99814 original sequence version: 115 DIP protein Q99814 original sequence version: 155 DIP protein Q99814 original sequence version: 157 DIP protein Q99814 original sequence version: 137 DIP protein Q99814 original sequence version: 135 4838989598234FC1 cdk2-cdkn1a 6 12 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 3 E cyclin-dependent kinase 2 (CDK2) immunoprecipitations (IPs) were performed on hypoxic HCT116 and WT8 cells at 48 hr and tested for coprecipitated p21 and p27. We observed substantially increased levels of p21 associated with CDK2 in hypoxic HCT116 cells Hypoxia (0.5% O2 for 48 h) substantially increased levels of p21 interacting with Cdk2. cdk2-cdkn1b 6 12 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 3 E Hypoxia (0.5% O2 for 48 h) increased levels of p27 interacting with Cdk2 but not to the same extent as p21. However, it should be noted that HCT116 cells express higher levels of p21 than p27. cyclin-dependent kinase 2 (CDK2) immunoprecipitations (IPs) were performed on hypoxic HCT116 and WT8 cells at 48 hr and tested for coprecipitated p21 and p27. We observed substantially increased levels of p21 associated with CDK2 in hypoxic HCT116 cells, while less change was observed for p27. cdk2-cdkn1b-1 1 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association 3 E Hypoxia (0.5% O2 for 48 h) almost totally abrogated the interaction between p27 and Cdk2, in contrast to the effect on the interaction in HCT116 cells. cyclin-dependent kinase 2 (CDK2) immunoprecipitations (IPs) were performed on hypoxic HCT116 and WT8 cells at 48 hr and tested for coprecipitated p21 and p27. We observed substantially increased levels of p21 associated with CDK2 in hypoxic HCT116 cells, while less change was observed for p27. However, it should be noted that HCT116 cells express higher levels of p21 than p27. In direct contrast, p27 association with CDK2 was almost completely abrogated in hypoxic WT8 cells myc-max-2 6 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 6 A hypoxic culture for 4 or 20 hr correlated with a substantial decrease in c-Myc coprecipitation with Sp1, Miz1, and Max in HCT116 cells and an increase in c-Myc coprecipitation with these proteins in WT8 cells, albeit less dramatically than the decrease seen in HCT116 cells Hypoxic conditions (0.5% O2 for 4 h and 20 h) correlated with a substantial decrease in the amount of Sp1, Miz1 and Max coimmunoprecipitated with c-Myc. mad1l1-max 6 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association 6 A This interaction increased under hypoxic conditions (0.5% O2 for 4 h and 20 h). When Mad1 IP was performed, the opposite result was observed: increased Mad1/Max association in hypoxic HCT116 cells and decreased association in hypoxic WT8 cells. mad1l1-max-1 1 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association 6 A This interaction decreased slightly under hypoxic conditions (0.5% O2 for 4 h and 20 h), in contrast to the situation in HCT116 cells. When Mad1 IP was performed, the opposite result was observed: increased Mad1/Max association in hypoxic HCT116 cells and decreased association in hypoxic WT8 cells. myc-max-3 1 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 6 A hypoxic culture for 4 or 20 hr correlated with a substantial decrease in c-Myc coprecipitation with Sp1, Miz1, and Max in HCT116 cells and an increase in c-Myc coprecipitation with these proteins in WT8 cells, albeit less dramatically than the decrease seen in HCT116 cells Hypoxic conditions (0.5% O2 for 4 h and 20 h) correlated with an increase in the amount of Sp1, Miz1 and Max coimmunoprecipitated with c-Myc, although not as marked as the decrease seen in HCT116 cells. sp1-myc 6 15 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with a substantial decrease in the amount of Myc coimmunoprecipitated with Sp1. IP was also performed for Sp1, Miz1, and Max. As before, we observed decreased coprecipitation of these proteins with c-Myc in hypoxic HCT116 cells and increased coprecipitation in hypoxic WT8 cells sp1-myc-1 1 15 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with an increase in the amount of Myc coimmunoprecipitated with Sp1, in contrast to the situation in HCT116 cells. IP was also performed for Sp1, Miz1, and Max. As before, we observed decreased coprecipitation of these proteins with c-Myc in hypoxic HCT116 cells and increased coprecipitation in hypoxic WT8 cells pias2-myc 6 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with a substantial decrease in the amount of c-Myc coimmunoprecipitated with Miz1. IP was also performed for Sp1, Miz1, and Max. As before, we observed decreased coprecipitation of these proteins with c-Myc in hypoxic HCT116 cells and increased coprecipitation in hypoxic WT8 cells pias2-myc-1 1 10 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with an increase in the amount of c-Myc coimmunoprecipitated with Miz1, in contrast to the situation in HCT116 cells. IP was also performed for Sp1, Miz1, and Max. As before, we observed decreased coprecipitation of these proteins with c-Myc in hypoxic HCT116 cells and increased coprecipitation in hypoxic WT8 cells max-myc-5 6 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with a substantial decrease in the amount of c-Myc coimmunoprecipitated with Max. IP was also performed for Sp1, Miz1, and Max. As before, we observed decreased coprecipitation of these proteins with c-Myc in hypoxic HCT116 cells and increased coprecipitation in hypoxic WT8 cells max-myc-6 1 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with an increase in the amount of c-Myc coimmunoprecipitated with Max, in contrast to the situation in HCT116. IP was also performed for Sp1, Miz1, and Max. As before, we observed decreased coprecipitation of these proteins with c-Myc in hypoxic HCT116 cells and increased coprecipitation in hypoxic WT8 cells max-hif1a 5 13 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 6 D When the HIF-1a overexpressing cell lines and a vector control (V1) were incubated at 0.5% O2 for 20 hr, increasing levels of HIF-1a/Max binding correlated with decreasing c-Myc/ Max binding and HIF-2a/Max binding, despite similar levels of c-Myc and Max in whole-cell lysates. Q16665 over-expressed Increasing levels of expression of HIF-1 alpha and HIF-1 alpha/Max binding correlated with decreasing levels of c-Myc/Max and HIF-2 alpha/Max binding. max-epas1 5 8 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association 6 D When the HIF-1a overexpressing cell lines and a vector control (V1) were incubated at 0.5% O2 for 20 hr, increasing levels of HIF-1a/Max binding correlated with decreasing c-Myc/ Max binding and HIF-2a/Max binding, despite similar levels of c-Myc and Max in whole-cell lysates. Increasing levels of expression of HIF-1 alpha and HIF-1 alpha/Max binding correlated with decreasing levels of c-Myc/Max and HIF-2 alpha/Max binding. HIF-2 alpha expression decreases with increasing HIF-1 alpha expression but the decrease in HIF-2 alpha/Max binding is greater than the decrease in expression. Increasing levels of HIF-1 alpha expression correlate with decreasing levels of the HIF-2 alpha/Max onteraction. max-myc-7 5 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 6 D When the HIF-1a overexpressing cell lines and a vector control (V1) were incubated at 0.5% O2 for 20 hr, increasing levels of HIF-1a/Max binding correlated with decreasing c-Myc/ Max binding and HIF-2a/Max binding, despite similar levels of c-Myc and Max in whole-cell lysates. Increasing levels of expression of HIF-1 alpha and HIF-1 alpha/Max binding correlated with decreasing levels of c-Myc/Max and HIF-2 alpha/Max binding. Increasing levels of HIF-1 alpha expression correlate with decreasing levels of the c-Myc/Max interaction. max-epas1-1 3 8 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association 6 E Q99814 over-expressed when HIF-2a overexpressing cell lines were incubated under hypoxia, a dose-dependent increase in c-Myc/Max binding was observed, correlating with the increase in HIF-2a/Max binding This interaction occurred under hypoxic conditions. max-myc-8 3 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 6 E when HIF-2a overexpressing cell lines were incubated under hypoxia, a dose-dependent increase in c-Myc/Max binding was observed, correlating with the increase in HIF-2a/Max binding This interaction occurred under hypoxic conditions. Increasing c-Myc/Max binding correlated with increasing levels of expression of HIF-2 alpha. max-myc-9 2 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association S6 B There was no difference in c-Myc/Max binding in any of these lines under normoxia There was no difference in c-Myc/Max binding under normoxia correlating with HIF-1 alpha overexpression, in contrast to the findings under hypoxic conditions. max-myc-10 4 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association S6 C There was no difference in c-Myc/Max binding in any of these lines under normoxia There was no difference in c-Myc/Max binding under normoxia correlating with HIF-2 alpha overexpression, in contrast to the findings under hypoxic conditions.