IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Farnesol, a mevalonate pathway intermediate, stimulates MCF-7 breast cancer cell growth through farnesoid-X-receptor-mediated estrogen receptor activation. Farnesol, a mevalonate pathway intermediate, stimulates MCF-7 breast cancer cell growth through farnesoid-X-receptor-mediated estrogen receptor activation. Breast Cancer Res. Treat. (0167-6806) 2007 imex curation Journe F., Laurent G., Chaboteaux C., Nonclercq D., Durbecq V., Larsimont D., Body JJ. Cancer - Interactions investigated in the context of cancer IBD - Inflammatory bowel disease MCF-7 cells were incubated in steroid-free medium with 5.0 E-5 M farnesol or vehicle for 10, 20, 30 or 60 min. Solubilized protein preparations were subjected to FXR immunoprecipitation, followed by Western blotting for ER. fabrice.journe@bordet.be. 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Journe F., Laurent G., Chaboteaux C., Nonclercq D., Durbecq V., Larsimont D., Body JJ. Cancer - Interactions investigated in the context of cancer IBD - Inflammatory bowel disease MCF-7 cells were incubated in steroid-free medium with 5.0 E-5 M farnesol or vehicle for 10, 20, 30 or 60 min. Solubilized protein preparations were subjected to FXR immunoprecipitation, followed by Western blotting for ER. fabrice.journe@bordet.be. Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Breast Cancer Res. Treat. (0167-6806) 2007 Only protein-protein interactions imex curation nr1h4_human Bile acid receptor NR1H4 BAR FXR HRR1 RIP14 Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 protein protein human Homo sapiens Human MVMQFQGLENPIQISPHCSCTPSGFFMEMMSMKPAKGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDEYITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ C23283576A8CF76B esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372 original sequence version: 214 DIP protein P03372 original sequence version: 139 DIP protein P03372 original sequence version: 167 5455C57AB0CCCAA7 nr1h4-esr1 1 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait physical association physical association 7 These coimmunoprecipitation experiments revealed a time-dependent increase in the amount of FXR/ER complexes in MCF-7 cells incubated with 50 lM farnesol, There was a time-dependent increase increase in the amount of FXR/ER complexes upon incubation with 5.0 E-5 M. The amount of FXR/ER complexes increased upon incubation with farnesol with time, up to 30 min and then decreased until measurements stopped at 60 min. These data suggest involvement of FXR in the activation of ER by farnesol, indicating crosstalk between the two nuclear receptors.