IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Cancer Cell (1535-6108) 2006 imex curation Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Only protein-protein interactions 14 human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Accepted 2007-JAN-29 by JYOTI. Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Cancer Cell (1535-6108) 2006 imex curation Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Only protein-protein interactions 14 human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Accepted 2007-JAN-29 by JYOTI. Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Cancer Cell (1535-6108) 2006 imex curation Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Only protein-protein interactions 14 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Accepted 2007-JAN-29 by JYOTI. Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Cancer Cell (1535-6108) 2006 imex curation Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Only protein-protein interactions 14 human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Accepted 2007-JAN-29 by JYOTI. Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Cancer Cell (1535-6108) 2006 imex curation Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Only protein-protein interactions 14 human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bruno T., De Nicola F., Iezzi S., Lecis D., D'Angelo C., Di Padova M., Corbi N., Dimiziani L., Zannini L., Jekimovs C., Scarsella M., Porrello A., Chersi A., Crescenzi M., Leonetti C., Khanna KK., Soddu S., Floridi A., Passananti C., Delia D., Fanciulli M. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirmed by email that where not specified otherwise the recombinant proteins used were human. fanciulli@ifo.it Accepted 2007-JAN-29 by JYOTI. Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation atr_human Serine/threonine-protein kinase ATR ATR FRP1 Ataxia telangiectasia and Rad3-related protein FRAP-related protein 1 protein protein human Homo sapiens Human MGEHGLELASMIPALRELGSATPEEYNTVVQKPRQILCQFIDRILTDVNVVAVELVKKTDSQPTSVMLLDFIQHIMKSSPLMFVNVSGSHEAKGSCIEFSNWIITRLLRIAATPSCHLLHKKICEVICSLLFLFKSKSPAIFGVLTKELLQLFEDLVYLHRRNVMGHAVEWPVVMSRFLSQLDEHMGYLQSAPLQLMSMQNLEFIEVTLLMVLTRIIAIVFFRRQELLLWQIGCVLLEYGSPKIKSLAISFLTELFQLGGLPAQPASTFFSSFLELLKHLVEMDTDQLKLYEEPLSKLIKTLFPFEAEAYRNIEPVYLNMLLEKLCVMFEDGVLMRLKSDLLKAALCHLLQYFLKFVPAGYESALQVRKVYVRNICKALLDVLGIEVDAEYLLGPLYAALKMESMEIIEEIQCQTQQENLSSNSDGISPKRRRLSSSLNPSKRAPKQTEEIKHVDMNQKSILWSALKQKAESLQISLEYSGLKNPVIEMLEGIAVVLQLTALCTVHCSHQNMNCRTFKDCQHKSKKKPSVVITWMSLDFYTKVLKSCRSLLESVQKLDLEATIDKVVKIYDALIYMQVNSSFEDHILEDLCGMLSLPWIYSHSDDGCLKLTTFAANLLTLSCRISDSYSPQAQSRCVFLLTLFPRRIFLEWRTAVYNWALQSSHEVIRASCVSGFFILLQQQNSCNRVPKILIDKVKDDSDIVKKEFASILGQLVCTLHGMFYLTSSLTEPFSEHGHVDLFCRNLKATSQHECSSSQLKASVCKPFLFLLKKKIPSPVKLAFIDNLHHLCKHLDFREDETDVKAVLGTLLNLMEDPDKDVRVAFSGNIKHILESLDSEDGFIKELFVLRMKEAYTHAQISRNNELKDTLILTTGDIGRAAKGDLVPFALLHLLHCLLSKSASVSGAAYTEIRALVAAKSVKLQSFFSQYKKPICQFLVESLHSSQMTALPNTPCQNADVRKQDVAHQREMALNTLSEIANVFDFPDLNRFLTRTLQVLLPDLAAKASPAASALIRTLGKQLNVNRREILINNFKYIFSHLVCSCSKDELERALHYLKNETEIELGSLLRQDFQGLHNELLLRIGEHYQQVFNGLSILASFASSDDPYQGPRDIISPELMADYLQPKLLGILAFFNMQLLSSSVGIEDKKMALNSLMSLMKLMGPKHVSSVRVKMMTTLRTGLRFKDDFPELCCRAWDCFVRCLDHACLGSLLSHVIVALLPLIHIQPKETAAIFHYLIIENRDAVQDFLHEIYFLPDHPELKKIKAVLQEYRKETSESTDLQTTLQLSMKAIQHENVDVRIHALTSLKETLYKNQEKLIKYATDSETVEPIISQLVTVLLKGCQDANSQARLLCGECLGELGAIDPGRLDFSTTETQGKDFTFVTGVEDSSFAYGLLMELTRAYLAYADNSRAQDSAAYAIQELLSIYDCREMETNGPGHQLWRRFPEHVREILEPHLNTRYKSSQKSTDWSGVKKPIYLSKLGSNFAEWSASWAGYLITKVRHDLASKIFTCCSIMMKHDFKVTIYLLPHILVYVLLGCNQEDQQEVYAEIMAVLKHDDQHTINTQDIASDLCQLSTQTVFSMLDHLTQWARHKFQALKAEKCPHSKSNRNKVDSMVSTVDYEDYQSVTRFLDLIPQDTLAVASFRSKAYTRAVMHFESFITEKKQNIQEHLGFLQKLYAAMHEPDGVAGVSAIRKAEPSLKEQILEHESLGLLRDATACYDRAIQLEPDQIIHYHGVVKSMLGLGQLSTVITQVNGVHANRSEWTDELNTYRVEAAWKLSQWDLVENYLAADGKSTTWSVRLGQLLLSAKKRDITAFYDSLKLVRAEQIVPLSAASFERGSYQRGYEYIVRLHMLCELEHSIKPLFQHSPGDSSQEDSLNWVARLEMTQNSYRAKEPILALRRALLSLNKRPDYNEMVGECWLQSARVARKAGHHQTAYNALLNAGESRLAELYVERAKWLWSKGDVHQALIVLQKGVELCFPENETPPEGKNMLIHGRAMLLVGRFMEETANFESNAIMKKYKDVTACLPEWEDGHFYLAKYYDKLMPMVTDNKMEKQGDLIRYIVLHFGRSLQYGNQFIYQSMPRMLTLWLDYGTKAYEWEKAGRSDRVQMRNDLGKINKVITEHTNYLAPYQFLTAFSQLISRICHSHDEVFVVLMEIIAKVFLAYPQQAMWMMTAVSKSSYPMRVNRCKEILNKAIHMKKSLEKFVGDATRLTDKLLELCNKPVDGSSSTLSMSTHFKMLKKLVEEATFSEILIPLQSVMIPTLPSILGTHANHASHEPFPGHWAYIAGFDDMVEILASLQKPKKISLKGSDGKFYIMMCKPKDDLRKDCRLMEFNSLINKCLRKDAESRRRELHIRTYAVIPLNDECGIIEWVNNTAGLRPILTKLYKEKGVYMTGKELRQCMLPKSAALSEKLKVFREFLLPRHPPIFHEWFLRTFPDPTSWYSSRSAYCRSTAVMSMVGYILGLGDRHGENILFDSLTGECVHVDFNCLFNKGETFEVPEIVPFRLTHNMVNGMGPMGTEGLFRRACEVTMRLMRDQREPLMSVLKTFLHDPLVEWSKPVKGHSKAPLNETGEVVNEKAKTHVLDIEQRLQGVIKTRNRVTGLPLSIEGHVHYLIQEATDENLLCQMYLGWTPYM DIP protein Q13535 original sequence version: 70 DIP protein Q13535 original sequence version: 92 DIP protein Q13535 original sequence version: 108 DIP protein Q13535 original sequence version: 127 11BC22297FB9A802 tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS DIP protein Q04206 original sequence version: 170 DIP protein Q04206 original sequence version: 173 DIP protein Q04206 original sequence version: 99 DIP protein Q04206-1 original sequence version: 99 DIP protein Q04206 original sequence version: 168 DIP protein Q04206 original sequence version: 128 DIP protein Q04206 original sequence version: 131 DIP protein Q04206 original sequence version: 153 DIP protein Q04206 original sequence version: 185 DIP protein Q04206 original sequence version: 181 DIP protein Q04206 original sequence version: 183 8147A6AF9F2445C6 atm_human Serine-protein kinase ATM ATM Ataxia telangiectasia mutated protein protein human Homo sapiens Human MSLVLNDLLICCRQLEHDRATERKKEVEKFKRLIRDPETIKHLDRHSDSKQGKYLNWDAVFRFLQKYIQKETECLRIAKPNVSASTQASRQKKMQEISSLVKYFIKCANRRAPRLKCQELLNYIMDTVKDSSNGAIYGADCSNILLKDILSVRKYWCEISQQQWLELFSVYFRLYLKPSQDVHRVLVARIIHAVTKGCCSQTDGLNSKFLDFFSKAIQCARQEKSSSGLNHILAALTIFLKTLAVNFRIRVCELGDEILPTLLYIWTQHRLNDSLKEVIIELFQLQIYIHHPKGAKTQEKGAYESTKWRSILYNLYDLLVNEISHIGSRGKYSSGFRNIAVKENLIELMADICHQVFNEDTRSLEISQSYTTTQRESSDYSVPCKRKKIELGWEVIKDHLQKSQNDFDLVPWLQIATQLISKYPASLPNCELSPLLMILSQLLPQQRHGERTPYVLRCLTEVALCQDKRSNLESSQKSDLLKLWNKIWCITFRGISSEQIQAENFGLLGAIIQGSLVEVDREFWKLFTGSACRPSCPAVCCLTLALTTSIVPGTVKMGIEQNMCEVNRSFSLKESIMKWLLFYQLEGDLENSTEVPPILHSNFPHLVLEKILVSLTMKNCKAAMNFFQSVPECEHHQKDKEELSFSEVEELFLQTTFDKMDFLTIVRECGIEKHQSSIGFSVHQNLKESLDRCLLGLSEQLLNNYSSEITNSETLVRCSRLLVGVLGCYCYMGVIAEEEAYKSELFQKAKSLMQCAGESITLFKNKTNEEFRIGSLRNMMQLCTRCLSNCTKKSPNKIASGFFLRLLTSKLMNDIADICKSLASFIKKPFDRGEVESMEDDTNGNLMEVEDQSSMNLFNDYPDSSVSDANEPGESQSTIGAINPLAEEYLSKQDLLFLDMLKFLCLCVTTAQTNTVSFRAADIRRKLLMLIDSSTLEPTKSLHLHMYLMLLKELPGEEYPLPMEDVLELLKPLSNVCSLYRRDQDVCKTILNHVLHVVKNLGQSNMDSENTRDAQGQFLTVIGAFWHLTKERKYIFSVRMALVNCLKTLLEADPYSKWAILNVMGKDFPVNEVFTQFLADNHHQVRMLAAESINRLFQDTKGDSSRLLKALPLKLQQTAFENAYLKAQEGMREMSHSAENPETLDEIYNRKSVLLTLIAVVLSCSPICEKQALFALCKSVKENGLEPHLVKKVLEKVSETFGYRRLEDFMASHLDYLVLEWLNLQDTEYNLSSFPFILLNYTNIEDFYRSCYKVLIPHLVIRSHFDEVKSIANQIQEDWKSLLTDCFPKILVNILPYFAYEGTRDSGMAQQRETATKVYDMLKSENLLGKQIDHLFISNLPEIVVELLMTLHEPANSSASQSTDLCDFSGDLDPAPNPPHFPSHVIKATFAYISNCHKTKLKSILEILSKSPDSYQKILLAICEQAAETNNVYKKHRILKIYHLFVSLLLKDIKSGLGGAWAFVLRDVIYTLIHYINQRPSCIMDVSLRSFSLCCDLLSQVCQTAVTYCKDALENHLHVIVGTLIPLVYEQVEVQKQVLDLLKYLVIDNKDNENLYITIKLLDPFPDHVVFKDLRITQQKIKYSRGPFSLLEEINHFLSVSVYDALPLTRLEGLKDLRRQLELHKDQMVDIMRASQDNPQDGIMVKLVVNLLQLSKMAINHTGEKEVLEAVGSCLGEVGPIDFSTIAIQHSKDASYTKALKLFEDKELQWTFIMLTYLNNTLVEDCVKVRSAAVTCLKNILATKTGHSFWEIYKMTTDPMLAYLQPFRTSRKKFLEVPRFDKENPFEGLDDINLWIPLSENHDIWIKTLTCAFLDSGGTKCEILQLLKPMCEVKTDFCQTVLPYLIHDILLQDTNESWRNLLSTHVQGFFTSCLRHFSQTSRSTTPANLDSESEHFFRCCLDKKSQRTMLAVVDYMRRQKRPSSGTIFNDAFWLDLNYLEVAKVAQSCAAHFTALLYAEIYADKKSMDDQEKRSLAFEEGSQSTTISSLSEKSKEETGISLQDLLLEIYRSIGEPDSLYGCGGGKMLQPITRLRTYEHEAMWGKALVTYDLETAIPSSTRQAGIIQALQNLGLCHILSVYLKGLDYENKDWCPELEELHYQAAWRNMQWDHCTSVSKEVEGTSYHESLYNALQSLRDREFSTFYESLKYARVKEVEEMCKRSLESVYSLYPTLSRLQAIGELESIGELFSRSVTHRQLSEVYIKWQKHSQLLKDSDFSFQEPIMALRTVILEILMEKEMDNSQRECIKDILTKHLVELSILARTFKNTQLPERAIFQIKQYNSVSCGVSEWQLEEAQVFWAKKEQSLALSILKQMIKKLDASCAANNPSLKLTYTECLRVCGNWLAETCLENPAVIMQTYLEKAVEVAGNYDGESSDELRNGKMKAFLSLARFSDTQYQRIENYMKSSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRRPQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDGKERRQLVKGRDDLRQDAVMQQVFQMCNTLLQRNTETRKRKLTICTYKVVPLSQRSGVLEWCTGTVPIGEFLVNNEDGAHKRYRPNDFSAFQCQKKMMEVQKKSFEEKYEVFMDVCQNFQPVFRYFCMEKFLDPAIWFEKRLAYTRSVATSSIVGYILGLGDRHVQNILINEQSAELVHIDLGVAFEQGKILPTPETVPFRLTRDIVDGMGITGVEGVFRRCCEKTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKALYLQQRPEDETELHPTLNADDQECKRNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVLSVGGQVNLLIQQAIDPKNLSRLFPGWKAWV DIP protein Q13315 original sequence version: 122 DIP protein Q13315 original sequence version: 189 DIP protein Q13315 original sequence version: 195 DIP protein Q13315 original sequence version: 166 DIP protein Q13315 original sequence version: 196 C0B4866E1E3199E2 chk2_human Serine/threonine-protein kinase Chk2 CHEK2 CHK2 RAD53 Cds1 homolog Checkpoint kinase 2 CDS1 CHK2 checkpoint homolog protein protein human Homo sapiens Human MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAAVL DIP protein O96017 original sequence version: 175 DIP protein O96017-1 original sequence version: 127 DIP protein O96017 original sequence version: 144 28890ACF3C1F3408 p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 chk1_human Serine/threonine-protein kinase Chk1 CHEK1 CHK1 CHK1 checkpoint homolog Checkpoint kinase-1 Cell cycle checkpoint kinase protein protein human Homo sapiens Human MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT DIP protein O14757 original sequence version: 102 0ABD0FAB67E60F67 aatf_human Protein AATF Apoptosis-antagonizing transcription factor Rb-binding protein Che-1 AATF CHE1 DED HSPC277 protein protein human Homo sapiens Human MAGPQPLALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLRSLVGLQEELLFQYPDTRYLVDGTKPNAGSEEISSEDDELVEEKKQQRRRVPAKRKLEMEDYPSFMAKRFADFTVYRNRTLQKWHDKTKLASGKLGKGFGAFERSILTQIDHILMDKERLLRRTQTKRSVYRVLGKPEPAAQPVPESLPGEPEILPQAPANAHLKDLDEEIFDDDDFYHQLLRELIERKTSSLDPNDQVAMGRQWLAIQKLRSKIHKKVDRKASKGRKLRFHVLSKLLSFMAPIDHTTMNDDARTELYRSLFGQLHPPDEGHGD EC493EF3B4C3A199 atm-aatf 2 8 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association 2 D low levels of Che-1 coprecipitated with ATM in control cells, increasing amounts of Che-1 were coprecipitated after Dox treatment The interaction was stimulated by doxorubicin, as it causes DNA damage. atm-aatf-1 3 8 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:04:35 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'asp2870ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation 2 E, S2B, S2C The presence of a specific phosphorylation signal on GST-Che-1 and His-p53 positive control, but not on GST alone (Figure 2E), indicated that Che-1 can be phosphorylated by ATM in vitro. Che-1 is phosphorylated. atr-aatf-2 3 6 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q13535:p.Lys2327Arg mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K R Wed May 02 20:32:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys2327arg' Tue Jun 30 18:21:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys2327Arg' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation S2B The presence of a specific phosphorylation signal on GST-Che-1 and His-p53 positive control, but not on GST alone (Figure 2E), indicated that Che-1 can be phosphorylated by ATM in vitro. Similar results were obtained by performing the kinase assay with ATR Che-1 is phosphorylated. chek2-aatf 5 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 3 C A strong interaction between Che-1 and Chk2 was detected after DNA damage Doxorubicin (Dox) stimulated this interaction, as it causes DNA damage. chek2-aatf-1 1 12 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2E Doxorubicin (Dox) and ionizing radiation (IR) stimulated this interaction, as they cause DNA damage. A strong interaction between Che-1 and Chk2 was detected after DNA damage (Figure 3C) and confirmed in U2OS cells transfected with Myc-Che-1 and Flag-Chk2 and treated with Dox or IR atm-tp53 3 8 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:26:42 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'asp2870ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 10 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 2 E The presence of a specific phosphorylation signal on GST-Che-1 and His-p53 positive control, but not on GST alone (Figure 2E), indicated that Che-1 can be phosphorylated by ATM in vitro. p53 is phosphorylated. chek2-aatf-2 3 9 enzyme enzyme unspecified role unspecified role region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O96017:p.Asp347Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:24:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp347ala' Tue Jun 30 17:31:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp347Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation 3 D, S3F Che-1 was phosphorylated by Chk2 as efficiently as p53. Che-1 is phosphorylated. chek1-aatf 3 11 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation 3 G A kinase assay was performed with immunoprecipitated Flag-Chk1 from transfected 293 cells. GST-Che-1 was strongly phosphorylated by Chk1 Che-1 is phosphorylated. chek2-tp53 3 9 enzyme enzyme unspecified role unspecified role region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 10 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation 3 D The presence of a specific phosphorylation signal on GST-Che-1 and His-p53 positive control, but not on GST alone Che-1 is phosphorylated. p53 is phosphorylated. rela-aatf 5 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 6 F Doxorubicin (Dox) stimulated this interaction, as it causes DNA damage. coimmunoprecipitation of Che-1 with antip65 Ab demonstrated increased amounts of Che-1 associated to p65 after DNA damage. rela-aatf-1 5 7 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey Q9NY61:p.[Ser143Ala;Ser189Ala;Ser477Ala;Ser510Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:45:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser143ala,ser189ala,ser477ala,ser510ala' Tue Jun 30 20:04:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser143Ala;Ser189Ala;Ser477Ala;Ser510Ala]' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6 G The interaction occurred in the presence of doxorubicin (Dox), which causes DNA damage. Myc-Che-1 strongly interacted with p65 whereas Myc-Che-1S4A did not bind this protein, As the S4A mutant of Che-1 did not bind the p65 subunit of NF-kB, this suggests that phosphorylation of Che-1 regulates the interaction. rela-aatf-2 4 12 unspecified role unspecified role bait bait Q9NY61:p.[Ser143Ala;Ser189Ala;Ser477Ala;Ser510Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:16:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser143ala,ser189ala,ser477ala,ser510ala' Tue Jun 30 16:21:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser143Ala;Ser189Ala;Ser477Ala;Ser510Ala]' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association 6 G The interaction occurred in the presence of doxorubicin (Dox), which causes DNA damage. Myc-Che-1 strongly interacted with p65 whereas Myc-Che-1S4A did not bind this protein, As the S4A mutant of Che-1 did not bind the p65 subunit of NF-kB, this suggests that phosphorylation of Che-1 regulates the interaction. atm-aatf-2 3 8 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 enzyme target enzyme target substrate substrate unspecified role unspecified role Q9NY61:p.Ser189Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:26:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser189ala' Tue Jun 30 17:54:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser189Ala' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation 2 F substitution of Che-1 Ser187 with an alanine (Che-1S187A) prevented Che-1 phosphorylation by ATM Che-1 is phosphorylated on Ser187. chek2-aatf-3 3 9 enzyme enzyme unspecified role unspecified role region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 enzyme target enzyme target substrate substrate unspecified role unspecified role Q9NY61:p.[Ser141Ala;Arg474Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain R A Fri Jul 22 11:03:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser141ala,ser474ala' Wed May 02 16:38:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser141ala,arg474ala' Tue Jun 30 17:19:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser141Ala;Arg474Ala]' Q9NY61:p.[Ser141Ala;Lys508Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain K A Fri Jul 22 11:01:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser141ala,ser508ala' Wed May 02 16:31:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser141ala,lys508ala' Tue Jun 30 19:00:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser141Ala;Lys508Ala]' Q9NY61:p.[Ser143Ala;Ser477Ala;Ser510Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:36:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser143ala,ser477ala,ser510ala' Tue Jun 30 19:34:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser143Ala;Ser477Ala;Ser510Ala]' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation 3 E the phosphorylation of Che-1 with double Ser-to-Ala mutations at residues 141 and 474 (Myc-Che-1S141A/S474A) or 141 and 508 (Myc-Che-1S141A/S508A) was markedly attenuated, whereas the phosphorylation of Che-1 with a triple mutation (Myc-Che-1S3A) was abrogated Che-1 is phosphorylated on Ser141, Ser474 and Ser508.