IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A soluble ectodomain of LRIG1 inhibits cancer cell growth by attenuating basal and ligand-dependent EGFR activity. A soluble ectodomain of LRIG1 inhibits cancer cell growth by attenuating basal and ligand-dependent EGFR activity. Oncogene 2007 imex curation Goldoni S., Iozzo RA., Kay P., Campbell S., McQuillan A., Agnew C., Zhu JX., Keene DR., Reed CC., Iozzo RV. Cancer - Interactions investigated in the context of cancer iozzo@mail.jci.tju.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Goldoni S., Iozzo RA., Kay P., Campbell S., McQuillan A., Agnew C., Zhu JX., Keene DR., Reed CC., Iozzo RV. Cancer - Interactions investigated in the context of cancer dear checker:to save time for you as to why I have entered isoform 4 for EGFR: Uniprot confirms that isoform 4 is the one secreted by A431 cells -PMID:11161793 as none of the stated references confirm this - although clues given - e.g. Serine is the C terminal aa. Uniprot confirms that isoform 4 is the one secreted by A431 cells -PMID:11161793. iozzo@mail.jci.tju.edu Accepted 2020-FEB-25 AT 08:24 GMT AT 08:24 GMT by MARTA Oncogene 2007 Only protein-protein interactions imex curation imex curation A soluble ectodomain of LRIG1 inhibits cancer cell growth by attenuating basal and ligand-dependent EGFR activity. A soluble ectodomain of LRIG1 inhibits cancer cell growth by attenuating basal and ligand-dependent EGFR activity. Oncogene 2007 imex curation Goldoni S., Iozzo RA., Kay P., Campbell S., McQuillan A., Agnew C., Zhu JX., Keene DR., Reed CC., Iozzo RV. Cancer - Interactions investigated in the context of cancer iozzo@mail.jci.tju.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Goldoni S., Iozzo RA., Kay P., Campbell S., McQuillan A., Agnew C., Zhu JX., Keene DR., Reed CC., Iozzo RV. Cancer - Interactions investigated in the context of cancer dear checker: this is In vitro as LRIG1 was added to the cells. Uniprot confirms that isoform 4 is the one secreted by A431 cells -PMID:11161793. iozzo@mail.jci.tju.edu Accepted 2020-FEB-25 AT 08:24 GMT AT 08:24 GMT by MARTA Oncogene 2007 Only protein-protein interactions imex curation imex curation p00533-4 Epidermal growth factor receptor Proto-oncogene c-ErbB-1 Receptor tyrosine-protein kinase erbB-1 EGFR ERBB ERBB1 HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGS 3A00A5511A3B6AE2 lrig1_human Leucine-rich repeats and immunoglobulin-like domains protein 1 LRIG1 LIG1 protein protein human Homo sapiens Human MARPVRGGLGAPRRSPCLLLLWLLLLRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATGHPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKS 7B4F13CA343AF943 123qweasd-3 1 3 unspecified role unspecified role bait bait conditioned medium conditioned medium human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma 4 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range lrr_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain signal_peptide_tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 ebna Homo sapiens 293 ebna 293 ebna physical association physical association 5F egfr-lrig1-3 2 3 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma 4 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range lrr_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain signal_peptide_tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 ebna Homo sapiens 293 ebna 293 ebna colocalization colocalization 8i