MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. FEBS Lett. (0014-5793) 2006 imex curation Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer jeholee@unitel.co.kr 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis jeholee@unitel.co.kr Accepted 2007-FEB-15 by JYOTI. 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. FEBS Lett. (0014-5793) 2006 imex curation Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer jeholee@unitel.co.kr 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis jeholee@unitel.co.kr Accepted 2007-FEB-15 by JYOTI. 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. FEBS Lett. (0014-5793) 2006 imex curation Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer jeholee@unitel.co.kr 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. Only protein-protein interactions human-siha Homo sapiens SiHa cervical carcinoma cell line siha SiHa cells huamn cervical carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis jeholee@unitel.co.kr Accepted 2007-FEB-15 by JYOTI. 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. FEBS Lett. (0014-5793) 2006 imex curation Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer jeholee@unitel.co.kr 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis The approximate range for the BTB-PLZF construct was obtained by aligning the primers against the PLZF DNA sequence AF060568 using Align and consulting the information regarding the cds. jeholee@unitel.co.kr Accepted 2007-FEB-15 by JYOTI. 02-MAR-2006: Contacted by JYOTI. A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation ef1a1_human Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A LENG7 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Leukocyte receptor cluster member 7 protein protein human Homo sapiens Human MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK DIP protein P68104 original sequence version: null DIP protein P68104 original sequence version: 134 D465615545AF686A zbt16_human Zinc finger and BTB domain-containing protein 16 Zinc finger protein PLZF Promyelocytic leukemia zinc finger protein Zinc finger protein 145 ZBTB16 PLZF ZNF145 protein protein human Homo sapiens Human MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV 51F8E361FA31239E zbtb16-q8iub0 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association A homology search in GenBank using the BLAST program revealed that all six plasmids encoded CCS-3 (GenBank Accession No. AF322220). zbtb16-q8iub0-1 4 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region BTB domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range physical association physical association 1 A Cells containing only full-length PLZF cDNA construct and BTB-PLZF grew on the Ura, His, Trp and Leu deficient plates, whereas yeast cells transformed with both deletion mutant colonies (RD2, and ZF) failed to grow The PLZF construct containing the BTB domain bound to CCS-3 but those containing the RD2 or ZF domains did not. Bernd: remapped EEF1A1 from Q8IUB0 to P68104 zbtb16-q8iub0-2 1 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait region BTB domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 B As shown in Fig. 1B, GST-PLZF was co-purified with His-CCS-3 at an approximate molar ratio of 1:1. Bernd: remapped EEF1A1 from Q8IUB0 to P68104 zbtb16-q8iub0-3 3 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 C As shown in Fig. 1C, pcDNA4/HisMax- CCS-3 was co-immunoprecipitated with pEGFPC1-PLZF Q05516; P68104 transfection Bernd: remapped EEF1A1 from Q8IUB0 to P68104