IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Leukemia-associated mutations within the NOTCH1 heterodimerization domain fall into at least two distinct mechanistic classes. Leukemia-associated mutations within the NOTCH1 heterodimerization domain fall into at least two distinct mechanistic classes. Molecular and cellular biology 2006 imex curation Malecki MJ., Sanchez-Irizarry C., Mitchell JL., Histen G., Xu ML., Aster JC., Blacklow SC. Cancer - Interactions investigated in the context of cancer sblacklow@rics .bwh.harvard.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Malecki MJ., Sanchez-Irizarry C., Mitchell JL., Histen G., Xu ML., Aster JC., Blacklow SC. Cancer - Interactions investigated in the context of cancer The normal notch subunits disassociated completely only after treatment of 4M urea; the mutants conferred increased sensitivity to urea, as complete disassociation occurred by 2 to 3.5 M urea concentration The interaction was studied at increasing concentrations of denaturant urea sblacklow@rics .bwh.harvard.edu. Accepted 2020-APR-14 AT 18:24 BST AT 18:24 BST by PPORRAS Molecular and cellular biology 2006 Only protein-protein interactions imex curation imex curation Leukemia-associated mutations within the NOTCH1 heterodimerization domain fall into at least two distinct mechanistic classes. Leukemia-associated mutations within the NOTCH1 heterodimerization domain fall into at least two distinct mechanistic classes. Molecular and cellular biology 2006 imex curation Malecki MJ., Sanchez-Irizarry C., Mitchell JL., Histen G., Xu ML., Aster JC., Blacklow SC. Cancer - Interactions investigated in the context of cancer sblacklow@rics .bwh.harvard.edu. 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Malecki MJ., Sanchez-Irizarry C., Mitchell JL., Histen G., Xu ML., Aster JC., Blacklow SC. Cancer - Interactions investigated in the context of cancer sblacklow@rics .bwh.harvard.edu. Accepted 2020-APR-14 AT 18:24 BST AT 18:24 BST by PPORRAS Molecular and cellular biology 2006 Only protein-protein interactions imex curation imex curation notc1_human Neurogenic locus notch homolog protein 1 Translocation-associated notch protein TAN-1 NOTCH1 TAN1 protein protein human Homo sapiens Human MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK DIP protein P46531 original sequence version: 173 DIP protein P46531 original sequence version: 103 DIP protein P46531 original sequence version: 109 DIP protein P46531 original sequence version: 127 DIP protein P46531 original sequence version: 137 DIP protein P46531 original sequence version: 186 DIP protein P46531 original sequence version: 150 DIP protein P46531 original sequence version: 142 DIP protein P46531 original sequence version: 181 E173C872D195F028 notch1-notch1-1 1 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell n-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal region of hd domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell c-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P46531:p.Val1676Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V D Tue Jun 30 17:01:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1676Asp' P46531:p.Leu1678Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 18:25:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1678Pro' P46531:p.Ile1680Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 17:29:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1680Asn' P46531:p.Ala1701Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A P Tue Jun 30 16:56:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1701Pro' P46531:p.Ile1718Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 19:50:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1718Thr' P46531:p.Ala1720_Val1721insAlaArgLeuGlySerLeuAsnIleProTyrLysIleGluAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain AV AARLGSLNIPYKIEAV Tue Jun 30 19:09:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1720_Val1721insAlaArgLeuGlySerLeuAsnIleProTyrLysIleGluAla' c-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figures 6, S4 notch1-notch1-2 2 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P46531:p.Leu1574Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 17:02:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1574Pro' P46531:p.Val1576Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V E Tue Jun 30 19:27:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1576Glu' P46531:p.Phe1592Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F S Tue Jun 30 18:58:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe1592Ser' P46531:p.Leu1593Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 19:43:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1593Pro' P46531:p.Leu1596His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L H Tue Jun 30 18:18:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1596His' P46531:p.Arg1598Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 17:18:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1598Pro' P46531:p.Leu1600Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 17:52:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1600Pro' P46531:p.Ile1616Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 18:44:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1616Thr' P46531:p.Ile1616Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 17:09:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1616Asn' n-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 5-1 notch1-notch1-4 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P46531:p.Val1676Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V D Tue Jun 30 20:12:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1676Asp' P46531:p.Leu1678Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 16:37:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1678Pro' P46531:p.Ile1680Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 20:18:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1680Asn' P46531:p.Ala1701Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A P Tue Jun 30 17:50:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1701Pro' P46531:p.Ile1718Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 19:32:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1718Thr' P46531:p.Ala1720_Val1721insAlaArgLeuGlySerLeuAsnIleProTyrLysIleGluAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain AV AARLGSLNIPYKIEAV Tue Jun 30 17:33:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1720_Val1721insAlaArgLeuGlySerLeuAsnIleProTyrLysIleGluAla' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 5-2 notch1-notch1-8 2 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P46531:p.Val1676Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V D Tue Jun 30 16:49:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1676Asp' P46531:p.Leu1678Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 15:44:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1678Pro' P46531:p.Ile1680Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 17:27:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1680Asn' P46531:p.Ala1701Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A P Tue Jun 30 19:28:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1701Pro' P46531:p.Ile1718Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 16:37:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1718Thr' P46531:p.Ala1720_Val1721insAlaArgLeuGlySerLeuAsnIleProTyrLysIleGluAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain AV AARLGSLNIPYKIEAV Tue Jun 30 16:53:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1720_Val1721insAlaArgLeuGlySerLeuAsnIleProTyrLysIleGluAla' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 5-4 notch1-notch1-7 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P46531:p.Leu1574Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L P In higher urea concentrations of 2 to 3.5M, mutation resulted in disruption of subunits Tue Jun 30 20:01:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1574Pro' P46531:p.Val1576Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V E Tue Jun 30 18:40:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1576Glu' P46531:p.Phe1592Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F S In higher urea concentrations of 2 to 3.5M, mutation resulted in disruption of subunits Tue Jun 30 16:00:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe1592Ser' P46531:p.Leu1593Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 16:18:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1593Pro' P46531:p.Leu1596His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L H Tue Jun 30 18:23:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1596His' P46531:p.Arg1598Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 20:03:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1598Pro' P46531:p.Leu1600Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 16:55:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1600Pro' P46531:p.Ile1616Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 15:41:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1616Thr' P46531:p.Ile1616Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 15:38:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1616Asn' n-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 5-3 notch1-notch1-3 1 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell c-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal region of hd domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P46531:p.Leu1574Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P In higher urea concentrations of 2 to 3.5M, mutation resulted in disruption of subunits Tue Jun 30 17:37:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1574Pro' P46531:p.Val1576Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V E Tue Jun 30 19:28:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1576Glu' P46531:p.Phe1592Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F S In higher urea concentrations of 2 to 3.5M, mutation resulted in disruption of subunits Tue Jun 30 18:02:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe1592Ser' P46531:p.Leu1593Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 19:50:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1593Pro' P46531:p.Leu1596His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L H Tue Jun 30 16:24:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1596His' P46531:p.Arg1598Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 16:57:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1598Pro' P46531:p.Leu1600Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 16:06:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1600Pro' P46531:p.Ile1616Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 17:35:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1616Thr' P46531:p.Ile1616Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 19:42:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1616Asn' n-terminal region of hd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 6