IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural determinants of the BRCA1 : estrogen receptor interaction. Structural determinants of the BRCA1 : estrogen receptor interaction. Oncogene (0950-9232) 2005 imex curation Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu 25-OCT-2006: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu Accepted 2006-SEP-21 by ORCHARD. 25-OCT-2006: Contacted by JYOTI. Oncogene (0950-9232) 2005 Only protein-protein interactions imex curation Structural determinants of the BRCA1 : estrogen receptor interaction. Structural determinants of the BRCA1 : estrogen receptor interaction. Oncogene (0950-9232) 2005 imex curation Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu 25-OCT-2006: Contacted by JYOTI. Only protein-protein interactions human-hcc-1937 Homo sapiens hcc-1937 human breast carcinoma established from a primary ductal carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu Accepted 2006-SEP-21 by ORCHARD. 25-OCT-2006: Contacted by JYOTI. Oncogene (0950-9232) 2005 Only protein-protein interactions imex curation Structural determinants of the BRCA1 : estrogen receptor interaction. Structural determinants of the BRCA1 : estrogen receptor interaction. Oncogene (0950-9232) 2005 imex curation Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu 25-OCT-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu Accepted 2006-SEP-21 by ORCHARD. 25-OCT-2006: Contacted by JYOTI. Oncogene (0950-9232) 2005 Only protein-protein interactions imex curation Structural determinants of the BRCA1 : estrogen receptor interaction. Structural determinants of the BRCA1 : estrogen receptor interaction. Oncogene (0950-9232) 2005 imex curation Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu 25-OCT-2006: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma YX., Tomita Y., Fan S., Wu K., Tong Y., Zhao Z., Song LN., Goldberg ID., Rosen EM. Cancer - Interactions investigated in the context of cancer 35S-methionine labeled proteins were prepared by in vitro transcription, then incubated with GST fusion protein, recovered using GSH agarose beads, eluted in boiling sample buffer, and analysed by SDS-PAGE autorediography. emr36@georgetown.edu Accepted 2006-SEP-21 by ORCHARD. 25-OCT-2006: Contacted by JYOTI. Oncogene (0950-9232) 2005 Only protein-protein interactions imex curation esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372 original sequence version: 214 DIP protein P03372 original sequence version: 139 DIP protein P03372 original sequence version: 167 5455C57AB0CCCAA7 brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY DIP protein P38398 original sequence version: 183 DIP protein P38398 original sequence version: 111 DIP protein P38398 original sequence version: 149 DIP protein P38398-1 original sequence version: 149 DIP protein P38398 original sequence version: 142 DIP protein P38398 original sequence version: 212 DIP protein P38398 original sequence version: 163 DIP protein P38398 original sequence version: 185 DIP protein P38398 original sequence version: 148 DIP protein P38398 original sequence version: 210 89C6D83FF56312AF esr1-brca1-1 3 6 unspecified role unspecified role prey prey region 35S-methionine labeled 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 C These studies revealed that most of the BRCA1 : ER-a interaction was mediated by aa 338�379 of ER-a; but a smaller amount of binding could be ascribed to aa 420�595 of ER-a Most of the binding was mediated by ER-alpha 338-379 and some by 420-595. However, much fainter bands are seen for ER-alpha 283-337 and 380-419. None of the ER-alpha proteins captured the BRCA1 construct missing aa 1-302. E2 caused a slight reduction in binding of BRCA-1 1-302 to GST-ER 338-379 and 42-595 but significant binding was retained. esr1-brca1-2 3 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey region 35S-methionine labeled 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction 2 E GST-ER 338�379 captured both BRCA1 1�100 and BRCA1 101�200 The findings suggest the presence of at least two contact points for ER-a on BRCA1, one within aa 1�100 and the other within aa 101�200. The interaction of ER-alpha with BRCA1 1�100 appeared to be stronger than that with BRCA1 101�200, suggesting that the former contains the primary interaction site. esr1-brca1-3 3 6 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35S-methionine labeled 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 E GST-ER 420�595 captured BRCA1 1�100 but not BRCA1 101�200 brca1-esr1-4 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 3 A we performed immunoprecipitation (IP)�Western blotting assays to determine if ER-a binding BRCA1 protein fragments identified above associate with the endogenous ER-a protein in MCF-7 cells. MCF-7 cells were transfected with FLAG-tagged BRCA1 proteins encompassing aa 1�100, 101�200, and 201�300. Each of the FLAG-BRCA1 proteins was expressed well. Consistent with the GST capture assays, we found that IP of FLAG-BRCA1 1�100 and 101�200, but not 201�300, co-precipitated ER-a (Figure 3a) and vice versa The interaction of ER-alpha with BRCA1 1�100 appeared to be stronger than that with BRCA1 101�200, suggesting that the former contains the primary interaction site. esr1-brca1-5 4 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 3 B we performed immunoprecipitation (IP)�Western blotting assays to determine if ER-a binding BRCA1 protein fragments identified above associate with the endogenous ER-a protein in MCF-7 cells. MCF-7 cells were transfected with FLAG-tagged BRCA1 proteins encompassing aa 1�100, 101�200, and 201�300. Each of the FLAG-BRCA1 proteins was expressed well. Consistent with the GST capture assays, we found that IP of FLAG-BRCA1 1�100 and 101�200, but not 201�300, co-precipitated ER-a (Figure 3a) and vice versa The interaction of ER-alpha with BRCA1 1�100 appeared to be stronger than that with BRCA1 101�200, suggesting that the former contains the primary interaction site. esr1-brca1-6 3 6 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P38398:p.[Leu86Ala;Ile89Ala;Ile90Ala] Mutant 1 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 16:46:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala,ile89ala,ile90ala' Tue Jun 30 16:20:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu86Ala;Ile89Ala;Ile90Ala]' P38398:p.[Ile89Ala;Ile90Ala] Mutant 2 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 14:51:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile89ala,ile90ala' Tue Jun 30 19:35:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile89Ala;Ile90Ala]' P38398:p.Leu86Ala Mutant 3 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:56:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala' Tue Jun 30 16:01:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu86Ala' region 35S-methionine labeled 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 5 A, C, D, 10 G the binding of BRCA1 mutant 1 to GST-ER 282�420 was significantly less than that of BRCA1 mutant 2, mutant 3, or wildtype protein (Figure 5c and d). Mutant 1 (which contains three leucine or isoleucine to alanine substitutions) yielded about 28% of the wild-type control binding; whereas mutants 2 and 3 (which contain one or two leucine/isoleucine to alanine substitutions) exhibited 80�90% of control binding. Mutant 1 yielded about 28% of the wild-type control binding; whereas mutants 2 and 3 exhibited 80�90% of control binding esr1-brca1-7 4 6 unspecified role unspecified role prey prey P38398:p.[Leu86Ala;Ile89Ala;Ile90Ala] Mutant 1 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 19:11:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala,ile89ala,ile90ala' Tue Jun 30 18:49:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu86Ala;Ile89Ala;Ile90Ala]' n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait direct interaction direct interaction 8 A anti-ER-a IP co-precipitated smaller smaller quantities of the FLAG-BRCA1 1�100 mutant 1 than any of the other BRCA1 1�100 proteins brca1-esr1-5 1 6 unspecified role unspecified role bait bait P38398:p.[Leu86Ala;Ile89Ala;Ile90Ala] Mutant 1 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 19:15:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala,ile89ala,ile90ala' Tue Jun 30 17:19:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu86Ala;Ile89Ala;Ile90Ala]' n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey direct interaction direct interaction 8 B anti-FLAGIP of cells transfected with FLAG-BRCA1 mutant 1 brought down reduced anti-FLAGIP of cells transfected with FLAG-BRCA1 mutant 1 brought down reduced brca1-esr1 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait P38398:p.[Leu86Ala;Ile90Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 19:57:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala,ile90ala' Tue Jun 30 18:11:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu86Ala;Ile90Ala]' physical association physical association the amount of ER-a precipitated by anti-BRCA1 and the amount of BRCA1 precipitated by anti-ER-a were significantly lower for BRCA1-NES mutant than for wtBRCA1 9 D esr1-brca1-10 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey P38398:p.[Leu86Ala;Ile90Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 20:15:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala,ile90ala' Tue Jun 30 17:31:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu86Ala;Ile90Ala]' physical association physical association the amount of ER-a precipitated by anti-BRCA1 and the amount of BRCA1 precipitated by anti-ER-a were significantly lower for BRCA1-NES mutant than for wtBRCA1 9 E brca1-esr1-2 2 6 unspecified role unspecified role bait bait P38398:p.[Leu86Ala;Ile89Ala;Ile90Ala] Mutant 1 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:15:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala,ile89ala,ile90ala' Tue Jun 30 16:38:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu86Ala;Ile89Ala;Ile90Ala]' 5 unspecified role unspecified role prey prey physical association physical association Western blotting of HCC1937 cells showed a significantly reduced steady-state association of BRCA1 1� 1863 mutant 1 with ER-a, as compared with that of wtBRCA1, mutant 2, or mutant 3 10 E esr1-brca1-11 2 6 unspecified role unspecified role prey prey P38398:p.[Leu86Ala;Ile89Ala;Ile90Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 18:20:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu86ala,ile89ala,ile90ala' Tue Jun 30 18:00:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu86Ala;Ile89Ala;Ile90Ala]' 5 unspecified role unspecified role bait bait physical association physical association Western blotting of HCC1937 cells showed a significantly reduced steady-state association of BRCA1 1� 1863 mutant 1 with ER-a, as compared with that of wtBRCA1, mutant 2, or mutant 3 10 F